FastQCFastQC Report
Thu 26 May 2016
SRR937713_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937713_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686993
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37640.5478949567171718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29900.4352300532902082No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19220.27976995398788634No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8250.12008855985432165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.044.9044271
GTATCAA34250.038.71441
GGTATCG300.003952003531.66538226-27
TATCAAC43500.030.0275152
ATCAACG43550.029.993043
TCAACGC43750.029.855934
CAACGCA44600.029.2869265
AACGCAG46000.028.3935176
GTAATAC1501.0594467E-625.3323043
GTCGAAG1551.4026209E-624.5294191
ACGCAGA52850.024.4455557
CGCAGAG52950.024.3993858
GTACATG28100.023.5091761
AGTACTT35500.022.81245212-13
TAGACTG3550.022.7455545
GAGTACT34000.022.70128412-13
GCAGAGT56600.022.3224149
TACATGG28650.021.8839262
GTACTTT36650.021.25270514-15
GTGTAGC3151.4551915E-1121.1225531
Produced by FastQC (version 0.11.5)