FastQCFastQC Report
Thu 26 May 2016
SRR937712_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937712_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559822
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21960.39226754218305104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18020.3218880286948351No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11650.20810186094865868No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7250.12950544994659016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14400.047.545341
GTATCAA24750.039.7651941
GGGCCGT702.049124E-433.936636
TGCACCG902.4009601E-531.6855375
CTACCGT600.0039446431.6741856
ATCAACG30550.031.4234313
TCAACGC31200.030.771534
TATCAAC31700.030.58332
CAACGCA31450.030.5269225
AACGCAG32500.029.6762776
TACGCAT650.005824551529.2481885
TATAAGC2002.3646862E-1128.514433
ACGCAGA36600.026.4793267
CGCAGAG36650.026.3135788
GTACATG24000.024.7631971
TACATGG24350.023.4204752
GAGTACT21400.023.3053512-13
GCAGAGT41000.023.1720859
ACATGGG24450.021.3809643
AGAGTAC36900.020.65992710-11
Produced by FastQC (version 0.11.5)