##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937712_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 559822 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92192875592599 33.0 31.0 34.0 30.0 34.0 2 32.04861902533305 33.0 31.0 34.0 30.0 34.0 3 32.089149765461165 34.0 31.0 34.0 30.0 34.0 4 35.66967536109692 37.0 35.0 37.0 35.0 37.0 5 35.67564868833308 37.0 35.0 37.0 35.0 37.0 6 35.73409405132345 37.0 35.0 37.0 35.0 37.0 7 35.66025808203322 37.0 35.0 37.0 35.0 37.0 8 35.669996891869204 37.0 35.0 37.0 35.0 37.0 9 37.25571342319523 39.0 37.0 39.0 34.0 39.0 10-11 37.19601855589812 39.0 37.0 39.0 34.0 39.0 12-13 37.10281660956518 39.0 37.0 39.0 34.0 39.0 14-15 38.28130905895088 40.0 38.0 41.0 33.5 41.0 16-17 38.195562160829695 40.0 38.0 41.0 33.5 41.0 18-19 38.12737798800333 40.0 38.0 41.0 33.5 41.0 20-21 38.13609504449629 40.0 38.0 41.0 34.0 41.0 22-23 38.14545069682863 40.0 38.0 41.0 34.0 41.0 24-25 38.07922875485423 40.0 38.0 41.0 34.0 41.0 26-27 37.97517246553369 40.0 38.0 41.0 33.0 41.0 28-29 37.8296967607561 40.0 38.0 41.0 33.0 41.0 30-31 37.592380256581556 40.0 37.0 41.0 33.0 41.0 32-33 37.47702484003844 40.0 37.0 41.0 33.0 41.0 34-35 37.46052405943318 40.0 37.0 41.0 33.0 41.0 36-37 37.310429029227144 40.0 37.0 41.0 32.0 41.0 38-39 37.14211838763035 40.0 37.0 41.0 31.5 41.0 40-41 36.92056671584896 40.0 36.5 41.0 31.0 41.0 42-43 36.66951816827492 39.5 36.0 41.0 30.5 41.0 44-45 36.40547263237244 39.0 36.0 41.0 30.0 41.0 46-47 36.036646469770744 39.0 35.5 41.0 29.5 41.0 48-49 35.68379502770523 39.0 35.0 40.0 27.5 41.0 50-51 34.85140276730818 38.5 34.0 39.5 26.5 40.5 52-53 34.96435831389263 38.5 35.0 40.0 27.0 40.5 54-55 35.224483853796386 39.0 35.0 40.0 26.5 41.0 56-57 35.00804898699944 38.5 35.0 40.0 26.0 41.0 58-59 34.73636441583217 38.0 34.0 40.0 25.5 41.0 60-61 34.38569759673611 38.0 34.0 40.0 24.0 41.0 62-63 34.03386969429569 38.0 34.0 40.0 23.0 41.0 64-65 33.597719453683496 37.0 33.0 40.0 22.0 41.0 66-67 33.04989889643494 36.5 32.5 39.0 19.0 41.0 68-69 32.61311899139369 36.0 32.0 39.0 18.5 40.0 70-71 32.134542765378995 35.5 31.0 39.0 15.5 40.0 72-73 31.64892948115651 35.0 31.0 38.0 10.5 40.0 74-75 31.069077313860475 35.0 30.5 37.5 7.5 39.0 76-77 30.50153888200178 34.5 30.0 37.0 4.5 39.0 78-79 29.953343205518898 34.0 29.0 36.0 2.0 39.0 80-81 29.378290778140197 34.0 29.0 36.0 2.0 37.5 82-83 28.798100110392234 34.0 28.5 35.0 2.0 37.0 84-85 28.2216383064617 34.0 27.5 35.0 2.0 36.5 86-87 27.595229376480383 33.0 26.0 35.0 2.0 36.0 88-89 27.025726034346633 33.0 25.5 35.0 2.0 36.0 90-91 26.433874517257273 32.0 25.0 35.0 2.0 35.0 92-93 25.742854871727083 32.0 23.0 34.0 2.0 35.0 94-95 24.94569613198481 31.5 19.0 34.0 2.0 35.0 96-97 24.052633694281397 31.0 7.0 34.0 2.0 35.0 98-99 22.83120795538582 31.0 2.0 34.0 2.0 35.0 100-101 19.560136078967957 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12287673948268818 1101 2 -0.029985553693940403 1101 3 0.0977895592562561 1101 4 0.05946427469555715 1101 5 0.016005827704219655 1101 6 -0.0049724360743397256 1101 7 0.13335505053744612 1101 8 0.08524142409537916 1101 9 0.38047511532516154 1101 10-11 0.39444650810050774 1101 12-13 0.29720846727411754 1101 14-15 0.41534393811646453 1101 16-17 0.3889194701540646 1101 18-19 0.48008049874377434 1101 20-21 0.4163172350511175 1101 22-23 0.26861622856672085 1101 24-25 0.32112106257358874 1101 26-27 0.5310132666694614 1101 28-29 0.5638615088029795 1101 30-31 0.3646276111096469 1101 32-33 0.37531989649585284 1101 34-35 0.595479655167189 1101 36-37 0.42139496911406127 1101 38-39 0.2887578196809528 1101 40-41 0.4372917793202191 1101 42-43 0.3225106594086853 1101 44-45 0.7417967584210103 1101 46-47 0.7681315128061357 1101 48-49 1.0439812913762552 1101 50-51 0.6771517207375695 1101 52-53 1.0224978277424412 1101 54-55 0.8529382583217284 1101 56-57 1.053738555441754 1101 58-59 1.170967507706834 1101 60-61 1.1078568183749553 1101 62-63 0.7284007562972263 1101 64-65 0.971402407155324 1101 66-67 1.160302246962253 1101 68-69 1.0431107819568908 1101 70-71 0.9491130899752456 1101 72-73 1.0379206069526 1101 74-75 1.3718316013464396 1101 76-77 1.004105827780574 1101 78-79 1.4331835600249008 1101 80-81 1.4333292215380595 1101 82-83 1.256301884729627 1101 84-85 1.6720615598276005 1101 86-87 1.6956423961620075 1101 88-89 1.8759013908976847 1101 90-91 1.7918699736214272 1101 92-93 1.7713203717417443 1101 94-95 2.1431742043038717 1101 96-97 2.0708021997230865 1101 98-99 2.619215168026212 1101 100-101 3.4635948411801465 1102 1 0.18081263375048096 1102 2 0.19596682536049315 1102 3 0.12202650016450889 1102 4 0.1870938293352964 1102 5 0.1638268545757029 1102 6 0.09080353663170371 1102 7 0.3013157357561411 1102 8 0.14248779513062004 1102 9 0.2987407053258764 1102 10-11 0.2595410883681808 1102 12-13 0.16276538681911035 1102 14-15 0.2412931286241573 1102 16-17 0.2866787189324569 1102 18-19 0.37291942133382605 1102 20-21 0.4850782455015761 1102 22-23 0.32642856664833175 1102 24-25 0.2545681207406574 1102 26-27 0.39498189136953954 1102 28-29 0.33358267039054823 1102 30-31 0.47628653226485085 1102 32-33 0.4643680355802573 1102 34-35 0.5088189404544394 1102 36-37 0.5682654104886424 1102 38-39 0.7302021516405546 1102 40-41 0.7790003620909047 1102 42-43 0.7096998757436097 1102 44-45 1.0464205497625088 1102 46-47 0.891896947365197 1102 48-49 0.9794909407158343 1102 50-51 0.7746694371003926 1102 52-53 0.8015813596956605 1102 54-55 0.9246885313410473 1102 56-57 0.916551331948547 1102 58-59 0.8495042038941278 1102 60-61 1.1713108913448451 1102 62-63 1.1231787138736138 1102 64-65 0.9580623247313937 1102 66-67 1.2995520616279777 1102 68-69 1.2384650693565433 1102 70-71 1.1551430304291337 1102 72-73 1.2247953528652893 1102 74-75 1.1885499490415192 1102 76-77 0.8080640099900798 1102 78-79 1.329607604855866 1102 80-81 1.6296540228517387 1102 82-83 1.6283773895164302 1102 84-85 1.747564353791681 1102 86-87 1.9295661420667187 1102 88-89 2.08627373344326 1102 90-91 1.9076281701793896 1102 92-93 2.2571393101151074 1102 94-95 2.1916620360123744 1102 96-97 2.5480798185163813 1102 98-99 3.181718655499207 1102 100-101 3.181932572050858 1103 1 0.019714635261053104 1103 2 -0.11311550092349165 1103 3 -0.07715684575693871 1103 4 -0.040960362507604486 1103 5 -0.004385003430343204 1103 6 0.05957241880692976 1103 7 0.17356365871686563 1103 8 0.0300633238315271 1103 9 0.14514365094520798 1103 10-11 0.26926304833796877 1103 12-13 0.22795137624508754 1103 14-15 0.37468389524047296 1103 16-17 0.09812131711976946 1103 18-19 0.17321209504983415 1103 20-21 0.1379861004864651 1103 22-23 0.009014480545616266 1103 24-25 -0.024628442703452436 1103 26-27 0.04510462550850747 1103 28-29 -0.10077816797802797 1103 30-31 -0.12384328268982614 1103 32-33 0.010737937392953256 1103 34-35 0.08865041704054022 1103 36-37 0.005215467135172958 1103 38-39 0.174058363876199 1103 40-41 0.35748884396401337 1103 42-43 0.11007043843243025 1103 44-45 -0.12133813603809784 1103 46-47 -0.28819887967407 1103 48-49 -0.08005399886120301 1103 50-51 -0.05494961501440088 1103 52-53 0.09251555531499633 1103 54-55 0.04149366729270554 1103 56-57 0.07840461744703475 1103 58-59 -0.03909804308472786 1103 60-61 -0.16148272647992457 1103 62-63 0.3930569238434032 1103 64-65 0.14802880887036451 1103 66-67 0.11906914999655527 1103 68-69 0.2623864250967287 1103 70-71 0.046594125595294855 1103 72-73 0.10733642159640411 1103 74-75 0.4713884120324572 1103 76-77 0.5747275296275411 1103 78-79 0.6002906675446908 1103 80-81 0.8534644458124667 1103 82-83 0.7926145496372747 1103 84-85 1.0396881264502902 1103 86-87 0.7869703150274461 1103 88-89 1.1150525287604829 1103 90-91 1.148047826523804 1103 92-93 1.6855168592087608 1103 94-95 1.7351122814806494 1103 96-97 1.449106536794325 1103 98-99 1.6431494523270018 1103 100-101 2.497557572050855 1104 1 0.32262099191634164 1104 2 0.3162147038546621 1104 3 0.23323671226354037 1104 4 0.23374633544461432 1104 5 0.10814699315668719 1104 6 0.15432497853389293 1104 7 0.2926389573516772 1104 8 0.27200018389978453 1104 9 0.41036336084282254 1104 10-11 0.37789946045401024 1104 12-13 0.3194242858014036 1104 14-15 0.5588642493360325 1104 16-17 0.44705476421874124 1104 18-19 0.4607440916368688 1104 20-21 0.47727790936018977 1104 22-23 0.4882326972691615 1104 24-25 0.391620490743648 1104 26-27 0.5698166562252354 1104 28-29 0.52584832519603 1104 30-31 0.7010527284023169 1104 32-33 0.9174732189629182 1104 34-35 0.9276835868357622 1104 36-37 0.8220856676471158 1104 38-39 0.8906245968113637 1104 40-41 1.0883922142711882 1104 42-43 1.0546443505484717 1104 44-45 1.3787365226649726 1104 46-47 1.3857798959231928 1104 48-49 1.4444873833913476 1104 50-51 1.4368885889105059 1104 52-53 1.241702308727966 1104 54-55 1.35979571507427 1104 56-57 1.540003380245679 1104 58-59 1.5179332069152736 1104 60-61 1.5872214117453325 1104 62-63 1.7603084170174625 1104 64-65 1.595354427471051 1104 66-67 1.7293000586996214 1104 68-69 1.7742651580318878 1104 70-71 2.026782905458777 1104 72-73 1.8374852057261002 1104 74-75 1.5572485869471357 1104 76-77 1.9126648163169655 1104 78-79 2.193323410718751 1104 80-81 2.2191001113414757 1104 82-83 2.2001657441765268 1104 84-85 2.3755429686004597 1104 86-87 2.646998578679323 1104 88-89 2.5205586081120153 1104 90-91 2.5216533086057105 1104 92-93 2.6577198242258255 1104 94-95 2.90275573284584 1104 96-97 3.2416060035178766 1104 98-99 3.709177076047137 1104 100-101 4.1290315481600715 1105 1 0.179776089235947 1105 2 0.3508227835953335 1105 3 0.3907759471300878 1105 4 0.3040906312162406 1105 5 0.001538113724471657 1105 6 0.1632858079282684 1105 7 0.17635523821477506 1105 8 0.10814909789010585 1105 9 0.22726969697031763 1105 10-11 0.41776265607855834 1105 12-13 0.3593517423538799 1105 14-15 0.6545773314329395 1105 16-17 0.46198114975575066 1105 18-19 0.4880264255937732 1105 20-21 0.5260091130387679 1105 22-23 0.5737961228050281 1105 24-25 0.37864692340533423 1105 26-27 0.3828530564708572 1105 28-29 0.4663962567081654 1105 30-31 0.49366129366999445 1105 32-33 0.3539191612423238 1105 34-35 0.19852554779367892 1105 36-37 0.5283489660891405 1105 38-39 0.6797559318678381 1105 40-41 0.6052464276460228 1105 42-43 0.41668327839059316 1105 44-45 0.538978286556052 1105 46-47 0.7313715962673442 1105 48-49 0.4903304153647312 1105 50-51 0.3616854529772269 1105 52-53 0.726972276026288 1105 54-55 0.6284706547403971 1105 56-57 0.629640234601851 1105 58-59 0.7961758847395402 1105 60-61 0.8870986019719567 1105 62-63 0.9320278966467512 1105 64-65 0.8643435316737111 1105 66-67 1.3225962158961337 1105 68-69 1.1596308027117956 1105 70-71 0.9413051611601482 1105 72-73 1.303155590006444 1105 74-75 1.219175415170529 1105 76-77 1.2904048308827711 1105 78-79 1.7688085807246061 1105 80-81 1.5904675838877793 1105 82-83 1.9254074366665677 1105 84-85 2.1441500772871116 1105 86-87 2.4636014604249326 1105 88-89 2.4976460172542048 1105 90-91 2.4928144321723344 1105 92-93 2.996464165694114 1105 94-95 2.817156606794459 1105 96-97 3.0604330273800997 1105 98-99 3.429920503582231 1105 100-101 3.6264932415069246 1106 1 0.2296629896027902 1106 2 0.03924794445465807 1106 3 -0.03529322530875589 1106 4 0.16797258567166296 1106 5 -0.13779711827628205 1106 6 0.263669057462387 1106 7 0.12087195703619358 1106 8 0.2555185059043552 1106 9 0.3076348974438119 1106 10-11 0.04483859105506127 1106 12-13 -0.07134396689215805 1106 14-15 -0.30258544999762194 1106 16-17 0.07329638714777076 1106 18-19 0.1160604844055797 1106 20-21 0.2996457643520145 1106 22-23 0.3885969385288064 1106 24-25 0.2840856679408077 1106 26-27 0.34073662830963514 1106 28-29 0.18452032641972949 1106 30-31 0.09911058635778858 1106 32-33 0.5538007875330067 1106 34-35 0.4253078925307321 1106 36-37 -0.07813756157631246 1106 38-39 0.039055300150714345 1106 40-41 0.23341233836177366 1106 42-43 0.571723537171934 1106 44-45 0.6603757995361335 1106 46-47 0.9390442050598153 1106 48-49 0.8627768834917333 1106 50-51 1.0522173142572981 1106 52-53 1.2656576246427278 1106 54-55 1.3499320426428483 1106 56-57 1.231901641256563 1106 58-59 0.8344598175595337 1106 60-61 1.2923556838842245 1106 62-63 1.0373277571767332 1106 64-65 1.1153016555090218 1106 66-67 1.498334293553981 1106 68-69 1.0704549650126935 1106 70-71 1.0674493426821279 1106 72-73 0.9005677766384217 1106 74-75 1.4553016648335806 1106 76-77 1.810065414781608 1106 78-79 1.8015752438752202 1106 80-81 1.8406668267648456 1106 82-83 1.7175795356316748 1106 84-85 1.6623925207079928 1106 86-87 1.7050047162879522 1106 88-89 2.1246135406652407 1106 90-91 1.9965531661596536 1106 92-93 2.0737674719538575 1106 94-95 2.0065955622929756 1106 96-97 2.027824586514214 1106 98-99 2.6771721238956268 1106 100-101 3.4935572219108018 1107 1 0.039756132551026724 1107 2 0.20888653159683201 1107 3 0.44158045099102594 1107 4 0.30181148708589234 1107 5 0.2026187228569185 1107 6 0.22600195810232293 1107 7 0.24045474950277423 1107 8 0.329622944427733 1107 9 0.22501323089100822 1107 10-11 0.30648145958458173 1107 12-13 0.29379233152964446 1107 14-15 0.37124343114833636 1107 16-17 0.37342619516854114 1107 18-19 0.5586033552124405 1107 20-21 0.721624311868581 1107 22-23 0.6193591621174264 1107 24-25 0.8279926616325852 1107 26-27 0.5556144493567459 1107 28-29 0.5271224079027306 1107 30-31 0.6006591732722271 1107 32-33 0.7956422395609692 1107 34-35 0.9350321074199428 1107 36-37 0.6749880789237892 1107 38-39 0.9320889363694249 1107 40-41 1.0177488371889467 1107 42-43 0.8111396288118371 1107 44-45 0.9824135577288402 1107 46-47 1.0674858256104756 1107 48-49 1.3351983724125063 1107 50-51 1.1390862081563213 1107 52-53 1.0191817985399325 1107 54-55 1.0866494938509703 1107 56-57 1.2026720632464958 1107 58-59 1.241510442687627 1107 60-61 1.1832030797531417 1107 62-63 1.282585209751268 1107 64-65 1.3124618203879805 1107 66-67 1.4671234352269096 1107 68-69 1.534564185936837 1107 70-71 1.4731905009340487 1107 72-73 1.5147191282359707 1107 74-75 1.4547323314091507 1107 76-77 1.5366601177034234 1107 78-79 1.7081476289268025 1107 80-81 1.69413178998591 1107 82-83 2.065954658038361 1107 84-85 2.2035325539570643 1107 86-87 2.6783257520193153 1107 88-89 2.243467586348558 1107 90-91 2.7249469627026635 1107 92-93 2.7263260563632343 1107 94-95 2.2740456317516546 1107 96-97 2.2961742027834866 1107 98-99 2.7147936765817455 1107 100-101 3.376377016495301 1108 1 0.30951866068478395 1108 2 0.268346363483289 1108 3 0.3489113039605769 1108 4 0.36402727873533536 1108 5 0.2621933864001633 1108 6 0.36529275778998027 1108 7 0.4265738988298651 1108 8 0.2856424198767229 1108 9 0.6173011368209202 1108 10-11 0.5423341994679163 1108 12-13 0.5146480570360552 1108 14-15 0.59491253224612 1108 16-17 0.6426834640124213 1108 18-19 0.6472763605865595 1108 20-21 0.6914465524638658 1108 22-23 0.6003545229185008 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-1.7194617215491625 2316 84-85 -1.9811672760920835 2316 86-87 -2.1115598756505207 2316 88-89 -2.9423759670022314 2316 90-91 -2.665365838730434 2316 92-93 -2.494660577231919 2316 94-95 -2.8342865532651125 2316 96-97 -2.8598314928666895 2316 98-99 -3.549580950215372 2316 100-101 -2.803660648288126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2251.0 3 402.0 4 392.0 5 418.0 6 477.0 7 579.0 8 706.0 9 879.0 10 1127.0 11 1419.0 12 1872.0 13 2430.0 14 2825.0 15 3572.0 16 4533.0 17 6068.0 18 5284.0 19 3706.0 20 3692.0 21 3918.0 22 4236.0 23 4715.0 24 5411.0 25 6031.0 26 6982.0 27 8117.0 28 9738.0 29 11517.0 30 14229.0 31 17772.0 32 22774.0 33 29631.0 34 40379.0 35 56906.0 36 82893.0 37 109790.0 38 75641.0 39 6509.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.839685355408626 19.51835725420631 15.223351072407675 22.41860631797739 2 14.384040061037204 19.81649437268514 42.66067040626992 23.138795160007735 3 18.76221335009842 24.795138123921955 30.343379849293306 26.099268676686314 4 12.090059336286119 17.00352650610825 38.66046498299433 32.245949174611304 5 13.201643466611262 37.51191047620412 35.01303883710767 14.273407220076944 6 30.524052953011005 36.72702278926907 18.506526050504398 14.242398207215528 7 27.696539923682156 32.950908869622495 21.340179984106417 18.012371222588936 8 25.267813514089184 35.827138794074 20.34469859009721 18.5603491017396 9 25.710347790287646 17.403118119820288 21.271233465194122 35.615300624697944 10-11 24.160990933743836 27.286030089588575 28.058407095486558 20.494571881181027 12-13 24.88573093270588 24.63557855903283 28.82131244523721 21.657378063024076 14-15 21.94576453215197 25.951850360227326 27.30818454378664 24.79420056383407 16-17 20.92330436541282 29.431747301652077 27.919902849630805 21.725045483304296 18-19 20.860446553847034 27.754542135555365 30.56903759699911 20.81597371359849 20-21 21.738749154374524 26.85115917803414 30.730745689547174 20.67934597804416 22-23 21.33231315802426 26.726982294589043 30.601590220635284 21.339114326751414 24-25 20.94131859773149 26.924987136177542 30.88614957829034 21.24754468780062 26-27 21.015001830146314 27.136371406044585 30.567023179765112 21.28160358404399 28-29 20.786846688492194 27.20625482169355 30.833689832871826 21.173208656942432 30-31 21.00294092903286 27.026654518702554 30.758126039304273 21.212278512960314 32-33 20.828948027865486 27.078097815580897 30.611938054427906 21.48101610212571 34-35 21.194292199028414 27.18678513766526 30.475107128665375 21.143815534640954 36-37 21.120323595344736 27.04911651132718 30.46771876915642 21.36284112417166 38-39 20.959970364988454 27.22510887198158 30.531488541934166 21.283432221095797 40-41 21.067352645117996 27.176378481154234 30.459501174365283 21.296767699362487 42-43 21.000758828540544 27.148343820916466 30.322985346018655 21.527912004524335 44-45 21.123505013490114 27.296921211470398 30.088994483147424 21.49057929189206 46-47 21.196167238967657 27.48798794364513 29.670249029236125 21.645595788151088 48-49 21.244147947407377 27.526730824822813 29.608276062050777 21.620845165719032 50-51 21.19662831353928 27.74759424139292 29.451335796739464 21.60444164832834 52-53 21.285538475308226 27.988006533754 29.155937437067863 21.570517553869905 54-55 21.270599816542497 27.804192675123613 29.270868290936754 21.65433921739714 56-57 21.188517439909816 28.034750088351473 29.117782579326203 21.65894989241251 58-59 21.35890368803881 27.66462973349901 29.323607581291704 21.65285899717048 60-61 21.390219788577078 27.603798991647764 29.542036298890988 21.463944920884163 62-63 21.294723177276946 27.63628653252062 29.544606647180867 21.52438364302157 64-65 21.160446505901636 27.628447393473483 29.419427802472313 21.79167829815256 66-67 21.18493559432432 27.83808618022495 29.225601938299032 21.7513762871517 68-69 21.162397444729756 27.724771627195377 29.362971843713375 21.749859084361496 70-71 21.218763896257975 27.701343077527362 29.19858239155662 21.88131063465804 72-73 21.228751338877053 27.664667336589886 29.128148318495693 21.978433006037363 74-75 21.104895843307244 27.81201593916415 29.087184865489814 21.99590335203879 76-77 21.11042107015211 27.663970734308975 29.1625129979257 22.063095197613215 78-79 21.15837709804399 27.64706777184306 29.12586608490697 22.068689045205986 80-81 21.08873486202904 27.903170674526688 29.01712132031204 21.990973143132234 82-83 21.224180846361833 27.836550419709432 29.01569146033115 21.923577273597584 84-85 21.19103152172649 27.652927298640016 28.96370076323858 22.192340416394913 86-87 21.130868766183454 27.58528131246343 29.188639775816128 22.095210145536992 88-89 21.20904912270969 27.474679868896345 29.009859617444338 22.306411390949624 90-91 21.031001814583234 27.379627276729313 29.269460242016017 22.31991066667144 92-93 21.151136167406754 27.179439919617842 29.466358761735446 22.203065151239958 94-95 20.896080598043206 27.235750171118738 29.40119626536154 22.466972965476515 96-97 20.92853244092093 27.296187263437638 29.252664262641265 22.52261603300017 98-99 20.81474767196416 27.443307953932315 29.005264085657046 22.736680288446482 100-101 21.166777004419473 26.823346275653527 29.74207804755855 22.267798672368443 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 677.0 1 395.5 2 111.0 3 118.5 4 111.0 5 86.5 6 135.5 7 283.0 8 460.5 9 574.5 10 569.0 11 548.5 12 583.0 13 649.5 14 744.5 15 807.0 16 813.5 17 853.5 18 914.5 19 965.0 20 1085.0 21 1259.0 22 1500.5 23 1835.5 24 2274.5 25 2972.0 26 3798.5 27 4677.5 28 5488.0 29 6507.5 30 7852.5 31 9102.5 32 10314.0 33 11827.0 34 13611.5 35 15006.0 36 16467.5 37 18006.5 38 18833.0 39 19179.5 40 19632.0 41 20348.5 42 20921.0 43 21374.0 44 21899.5 45 22356.5 46 22105.0 47 21790.5 48 21895.5 49 21140.5 50 19670.5 51 18263.5 52 17202.5 53 16273.5 54 15024.5 55 13553.0 56 12001.0 57 10484.5 58 9113.5 59 7732.5 60 6379.5 61 5107.5 62 3841.5 63 2780.5 64 2033.5 65 1464.5 66 1031.5 67 744.0 68 546.0 69 394.5 70 279.5 71 199.0 72 148.0 73 122.5 74 90.0 75 70.5 76 54.0 77 30.5 78 18.0 79 12.0 80 8.5 81 6.0 82 3.5 83 2.0 84 1.0 85 0.5 86 0.0 87 1.0 88 1.5 89 1.5 90 2.0 91 2.0 92 1.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30938405421723336 2 0.2632979768569295 3 0.27133624616395924 4 0.26419111789104394 5 0.2661560281660957 6 0.20327889936444082 7 0.19756279674610858 8 0.20202850191668068 9 0.20577969425996118 10-11 0.20667283529407562 12-13 0.20765529043160147 14-15 0.20488655322584678 16-17 0.1965803416085827 18-19 0.18827412999131868 20-21 0.1900604120595475 22-23 0.19550857236764543 24-25 0.1937222902994166 26-27 0.20068879036550902 28-29 0.20524380963949257 30-31 0.20631557888042984 32-33 0.20327889936444082 34-35 0.2050651814326697 36-37 0.19640171340175985 38-39 0.18282596968322073 40-41 0.18032517478770035 42-43 0.19041766847319327 44-45 0.19282914926530217 46-47 0.19872388009045733 48-49 0.2055117519497269 50-51 0.19836662367681157 52-53 0.21158511098170488 54-55 0.19818799546998866 56-57 0.17407318754889947 58-59 0.15960430279624596 60-61 0.1767526106512427 62-63 0.17032199520561891 64-65 0.17755643758194567 66-67 0.17532358499665965 68-69 0.17451975806595668 70-71 0.17746712347853424 72-73 0.18782755947426144 74-75 0.191578751817542 76-77 0.19273983516189075 78-79 0.18889932871519877 80-81 0.18666647612991272 82-83 0.17559152730689398 84-85 0.17032199520561891 86-87 0.17773506578876858 88-89 0.18354048251051228 90-91 0.1814862581320491 92-93 0.1771098670648885 94-95 0.17853889271947154 96-97 0.18523745047532966 98-99 0.18496950816509533 100-101 0.16648148875892693 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 559822.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.35054331360419 #Duplication Level Percentage of deduplicated Percentage of total 1 86.94136531606924 65.5107911299236 2 8.538708980342498 12.86792721731117 3 2.088379954131227 4.7208169256708326 4 0.8024993202813744 2.418750391679985 5 0.42944872587312966 1.6179597409937696 6 0.2713078142128433 1.2265914723698474 7 0.16973493976057177 0.8952733951182673 8 0.12771020367427444 0.7698426586838104 9 0.09484269747749688 0.6431803905831469 >10 0.5093658458134985 6.663790270222117 >50 0.02140409898351043 1.0698305929601504 >100 0.0042808118566938285 0.5401113103663109 >500 2.378228809240242E-4 0.1299206038527307 >1k 7.134686427720725E-4 0.9252139002643427 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2196 0.39226754218305104 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1802 0.3218880286948351 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1165 0.20810186094865868 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 725 0.12950544994659016 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.78628206822883E-4 0.0 0.0 0.0 0.0 3 1.78628206822883E-4 0.0 0.0 0.0 0.0 4 1.78628206822883E-4 0.0 0.0 0.0 0.0 5 1.78628206822883E-4 0.0 0.0 0.0 0.0 6 1.78628206822883E-4 0.0 0.0 0.0 0.0 7 1.78628206822883E-4 0.0 0.0 0.0 0.0 8 1.78628206822883E-4 0.0 0.0 0.0 0.0 9 1.78628206822883E-4 0.0 0.0 0.0 0.0 10-11 1.78628206822883E-4 0.0 0.0 0.0 0.0 12-13 1.78628206822883E-4 0.0 0.0 0.0 0.0 14-15 1.78628206822883E-4 0.0 0.0 0.0 0.0 16-17 1.78628206822883E-4 0.0 0.0 0.0 0.0 18-19 1.78628206822883E-4 0.0 0.0 8.93141034114415E-5 0.0 20-21 1.78628206822883E-4 0.0 0.0 1.78628206822883E-4 0.0 22-23 1.78628206822883E-4 0.0 0.0 1.78628206822883E-4 0.0 24-25 1.78628206822883E-4 0.0 0.0 3.57256413645766E-4 0.0 26-27 1.78628206822883E-4 0.0 0.0 3.57256413645766E-4 0.0 28-29 1.78628206822883E-4 0.0 0.0 8.038269307029735E-4 0.0 30-31 1.78628206822883E-4 0.0 0.0 0.0038405064466919842 0.0 32-33 1.78628206822883E-4 0.0 0.0 0.00660924365244667 0.0 34-35 1.78628206822883E-4 0.0 0.0 0.010003179582081446 0.0 36-37 1.78628206822883E-4 0.0 0.0 0.01554065399359082 0.0 38-39 1.78628206822883E-4 0.0 0.0 0.024204122024500646 0.0 40-41 1.78628206822883E-4 0.0 0.0 0.03152787850423885 0.0 42-43 1.78628206822883E-4 0.0 0.0 0.04028066063856012 0.0 44-45 1.78628206822883E-4 0.0 0.0 0.046532647877361014 0.0 46-47 1.78628206822883E-4 0.0 0.0 0.05537474411509373 0.0 48-49 1.78628206822883E-4 0.0 0.0 0.06135878904366031 0.0 50-51 1.78628206822883E-4 0.0 0.0 0.06725351986881545 0.0 52-53 1.78628206822883E-4 0.0 0.0 0.08377662899993213 0.0 54-55 1.78628206822883E-4 0.0 0.0 0.110660174126776 0.0 56-57 1.78628206822883E-4 0.0 0.0 0.15656762328025695 0.0 58-59 1.78628206822883E-4 0.0 0.0 0.2137286494635795 0.0 60-61 1.78628206822883E-4 0.0 0.0 0.2865196437439043 0.0 62-63 1.78628206822883E-4 0.0 0.0 0.3264430479688187 0.0 64-65 1.78628206822883E-4 0.0 0.0 0.3794956253952149 0.0 66-67 1.78628206822883E-4 0.0 0.0 0.4735433762874628 0.0 68-69 1.78628206822883E-4 0.0 0.0 0.6034060826476988 0.0 70-71 1.78628206822883E-4 0.0 0.0 0.7429147121763704 0.0 72-73 1.78628206822883E-4 0.0 0.0 0.9125221945546977 0.0 74-75 1.78628206822883E-4 0.0 0.0 1.0827548756569052 0.0 76-77 1.78628206822883E-4 0.0 0.0 1.2422698643497396 0.0 78-79 1.78628206822883E-4 0.0 0.0 1.3811532951545313 0.0 80-81 5.358846204686489E-4 0.0 0.0 1.4831500012503973 0.0 82-83 5.358846204686489E-4 0.0 0.0 1.6415932207022945 0.0 84-85 5.358846204686489E-4 0.0 0.0 1.9516917877468196 0.0 86-87 5.358846204686489E-4 0.0 0.0 2.3422623619650533 0.0 88-89 5.358846204686489E-4 0.0 0.0 2.7735601673389043 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1440 0.0 47.54534 1 GTATCAA 2475 0.0 39.765194 1 GGGCCGT 70 2.049124E-4 33.93663 6 TGCACCG 90 2.4009601E-5 31.685537 5 CTACCGT 60 0.00394464 31.674185 6 ATCAACG 3055 0.0 31.423431 3 TCAACGC 3120 0.0 30.77153 4 TATCAAC 3170 0.0 30.5833 2 CAACGCA 3145 0.0 30.526922 5 AACGCAG 3250 0.0 29.676277 6 TACGCAT 65 0.0058245515 29.248188 5 TATAAGC 200 2.3646862E-11 28.51443 3 ACGCAGA 3660 0.0 26.479326 7 CGCAGAG 3665 0.0 26.313578 8 GTACATG 2400 0.0 24.763197 1 TACATGG 2435 0.0 23.420475 2 GAGTACT 2140 0.0 23.30535 12-13 GCAGAGT 4100 0.0 23.172085 9 ACATGGG 2445 0.0 21.380964 3 AGAGTAC 3690 0.0 20.659927 10-11 >>END_MODULE