FastQCFastQC Report
Thu 26 May 2016
SRR937712_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937712_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559822
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30750.5492817359803652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25530.45603781201882027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16070.2870555283643729No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6940.1239679755350808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACG250.00501263257.0209661
GGTATCA18100.042.266921
GTATCAA29300.035.3542981
CGTACAC650.005841250529.231073
ATCAACG34850.029.0318643
TCAACGC35400.028.5808034
TATCAAC36050.028.3290042
CAACGCA36100.028.0266065
AACGCAG37100.027.3967596
GCGTTAC350.00839105527.14313912-13
GTCGATT458.9328724E-426.39623540-41
GTACATG23750.025.0091951
TACATGG24500.024.0410652
ACGCAGA41850.023.9488747
CGCAGAG41900.023.9202948
TGGTATC10000.023.7502462
GTGGTAT10350.023.4144081
ACATGGG24800.023.3671763
GAGTACT26550.023.34769812-13
GCAGAGT43950.022.5884029
Produced by FastQC (version 0.11.5)