##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937712_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 559822 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.464644119023546 33.0 31.0 34.0 31.0 34.0 2 32.68675221766919 34.0 31.0 34.0 31.0 34.0 3 32.78729667644358 34.0 31.0 34.0 31.0 34.0 4 36.33523334202657 37.0 37.0 37.0 35.0 37.0 5 36.21814612501831 37.0 35.0 37.0 35.0 37.0 6 36.30999496268457 37.0 37.0 37.0 35.0 37.0 7 36.28171633126244 37.0 37.0 37.0 35.0 37.0 8 36.274937390813506 37.0 37.0 37.0 35.0 37.0 9 37.99604874406508 39.0 38.0 39.0 35.0 39.0 10-11 37.94939820157121 39.0 38.0 39.0 35.0 39.0 12-13 37.89127615563518 39.0 38.0 39.0 35.0 39.0 14-15 39.190159550714334 40.0 38.5 41.0 36.0 41.0 16-17 39.164846147525466 40.0 38.0 41.0 36.0 41.0 18-19 39.15036833136247 40.0 38.5 41.0 36.0 41.0 20-21 39.07600451572107 40.0 38.5 41.0 36.0 41.0 22-23 38.93681027183641 40.0 38.0 41.0 35.5 41.0 24-25 38.891026076145636 40.0 38.0 41.0 35.0 41.0 26-27 38.844565772692036 40.0 38.0 41.0 35.0 41.0 28-29 38.73281596650364 40.0 38.0 41.0 35.0 41.0 30-31 38.79228933482428 40.0 38.0 41.0 35.0 41.0 32-33 38.71082415482064 40.0 38.0 41.0 35.0 41.0 34-35 38.589545426939274 40.0 38.0 41.0 35.0 41.0 36-37 38.44763246174677 40.0 38.0 41.0 35.0 41.0 38-39 38.31997760002287 40.0 38.0 41.0 34.5 41.0 40-41 38.142606935776016 40.0 38.0 41.0 34.0 41.0 42-43 37.93226150454966 40.0 38.0 41.0 33.5 41.0 44-45 37.62110813794385 40.0 38.0 41.0 33.0 41.0 46-47 37.250081275834106 40.0 37.0 41.0 32.5 41.0 48-49 36.90748041341712 40.0 37.0 41.0 31.5 41.0 50-51 36.66528825233735 40.0 37.0 41.0 31.0 41.0 52-53 36.45245006448478 40.0 36.0 41.0 31.0 41.0 54-55 36.25665390070415 39.0 36.0 41.0 30.5 41.0 56-57 35.99301653025426 39.0 35.5 40.5 30.0 41.0 58-59 35.76370167660435 39.0 35.0 40.0 29.0 41.0 60-61 35.44461096562836 38.5 35.0 40.0 28.5 41.0 62-63 35.09226146882402 38.0 35.0 40.0 28.0 41.0 64-65 34.70876099903184 38.0 34.0 40.0 27.0 41.0 66-67 34.30945461235893 37.0 34.0 39.5 26.0 41.0 68-69 33.86866629035657 36.5 34.0 39.0 26.0 40.5 70-71 33.47178120902716 36.0 33.0 39.0 25.5 40.0 72-73 33.02332348496486 35.5 33.0 38.5 25.0 40.0 74-75 32.53109738452579 35.0 32.0 37.5 23.5 39.5 76-77 31.313878339900896 34.0 30.5 36.0 22.5 39.0 78-79 31.827738995609316 35.0 32.0 36.0 23.0 39.0 80-81 31.60998763892809 35.0 32.0 36.0 22.0 38.5 82-83 31.24210463325843 35.0 31.5 36.0 20.5 37.0 84-85 30.839692795209906 34.0 31.0 35.0 19.5 37.0 86-87 30.425989868208106 34.0 31.0 35.0 18.0 36.0 88-89 30.01879883248604 34.0 30.5 35.0 12.5 36.0 90-91 29.53442433487787 34.0 30.0 35.0 7.0 35.5 92-93 29.090180450214532 34.0 29.5 35.0 2.0 35.0 94-95 28.664387966174964 34.0 29.0 35.0 2.0 35.0 96-97 28.16249718660574 34.0 29.0 35.0 2.0 35.0 98-99 27.409975849466438 33.0 28.0 35.0 2.0 35.0 100-101 24.95171143684957 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1697332676133101 1101 2 0.16436529130740496 1101 3 0.15315430029765054 1101 4 0.10188147664110403 1101 5 0.017031788411230764 1101 6 0.05197528370209881 1101 7 3.283090937742372E-4 1101 8 0.0703501236337587 1101 9 0.07679718507786504 1101 10-11 0.16255680859407562 1101 12-13 0.19554571198981563 1101 14-15 0.1243156826978975 1101 16-17 0.07661978335482189 1101 18-19 0.187328409541486 1101 20-21 0.17282658890042768 1101 22-23 0.12962205838892515 1101 24-25 0.10843548698428407 1101 26-27 -0.005273651279729563 1101 28-29 0.11940862331558577 1101 30-31 0.20377682728874902 1101 32-33 0.22630772808494726 1101 34-35 -0.04814393061594302 1101 36-37 0.039877290012036326 1101 38-39 0.17965490646912485 1101 40-41 0.2675771507839997 1101 42-43 0.11272441856815618 1101 44-45 0.13159213124816915 1101 46-47 0.2236106100137789 1101 48-49 0.2197921462272987 1101 50-51 0.5150742592236099 1101 52-53 0.3399187974691671 1101 54-55 0.37742547167061247 1101 56-57 0.33904976287547584 1101 58-59 0.0452573366043012 1101 60-61 0.12246059648257557 1101 62-63 0.33542907374651065 1101 64-65 0.29813508548233614 1101 66-67 0.313539494756526 1101 68-69 0.18397306472337505 1101 70-71 0.15956165762546703 1101 72-73 0.0030233901290870335 1101 74-75 0.12639408779364913 1101 76-77 0.023090159139997013 1101 78-79 0.2904607631249583 1101 80-81 0.1662328820205481 1101 82-83 0.09207825854996798 1101 84-85 0.3749522524437303 1101 86-87 0.5583769736819697 1101 88-89 0.5127527615382519 1101 90-91 0.5815546001609171 1101 92-93 0.9000133183019727 1101 94-95 0.6179458089575469 1101 96-97 0.907477882784729 1101 98-99 1.3290920846045005 1101 100-101 1.890910737796812 1102 1 0.07174524060631882 1102 2 0.1740066595128127 1102 3 0.11578453250356802 1102 4 -0.019657953725015886 1102 5 0.030240343282130766 1102 6 0.017124467832701384 1102 7 -0.04034160457632652 1102 8 0.05247831915544765 1102 9 0.13429863985231805 1102 10-11 0.0692818522373102 1102 12-13 0.08794620940310693 1102 14-15 0.03608261587392292 1102 16-17 0.10960131369783 1102 18-19 0.12571572231401262 1102 20-21 0.16657305160638458 1102 22-23 0.2032715095603166 1102 24-25 0.15401974831758025 1102 26-27 -0.06062283700460824 1102 28-29 0.03622940307469236 1102 30-31 0.26529784379744115 1102 32-33 0.08269956859383853 1102 34-35 0.23993728166953332 1102 36-37 0.25468171252636296 1102 38-39 0.25780220138578613 1102 40-41 0.39250441206149134 1102 42-43 0.5721003402574496 1102 44-45 0.55971033067582 1102 46-47 0.6215890713805479 1102 48-49 0.4225717618479976 1102 50-51 0.3485225130957019 1102 52-53 0.2502528774538675 1102 54-55 0.28196237511344435 1102 56-57 0.34260100680727135 1102 58-59 0.4752388430332175 1102 60-61 0.43793541094901656 1102 62-63 0.4134846979443978 1102 64-65 0.4318188932448521 1102 66-67 0.5769034912810156 1102 68-69 0.3293329320798293 1102 70-71 0.49019203331599215 1102 72-73 0.16863052928523814 1102 74-75 0.5392291841276133 1102 76-77 0.6370249055309607 1102 78-79 0.6340665312060132 1102 80-81 0.6929170325518363 1102 82-83 0.5696627749669965 1102 84-85 0.769895615882259 1102 86-87 0.838612368145057 1102 88-89 0.6853986176231963 1102 90-91 0.8481310575260643 1102 92-93 1.2049599500009087 1102 94-95 1.1354349878270433 1102 96-97 1.3386371586657653 1102 98-99 1.5266484620965493 1102 100-101 2.0198037540823144 1103 1 0.092317370515687 1103 2 0.09267168972429118 1103 3 0.0926208240443529 1103 4 0.0441637985408434 1103 5 -0.11103137121937579 1103 6 -0.050407631865184044 1103 7 -0.10619791122284994 1103 8 -0.014742224651200786 1103 9 0.07246423652905065 1103 10-11 -0.06012005486238792 1103 12-13 -0.022882718089341836 1103 14-15 -0.019327127328494953 1103 16-17 -0.18414868630217285 1103 18-19 -0.05290493478568692 1103 20-21 -0.02389381017609793 1103 22-23 0.12409146801952176 1103 24-25 0.11310443487347044 1103 26-27 -0.22750114138527522 1103 28-29 -0.12340758710657695 1103 30-31 0.10193398428083356 1103 32-33 0.012438994575717288 1103 34-35 0.014514792998838288 1103 36-37 0.042736376423640365 1103 38-39 -0.13449623139366906 1103 40-41 -0.21655667177536486 1103 42-43 -0.09229495808092025 1103 44-45 -0.26699378563836973 1103 46-47 -0.2129700298279147 1103 48-49 -0.3386985364903765 1103 50-51 -0.4244334914360195 1103 52-53 -0.3191037124252887 1103 54-55 -0.41453734165392575 1103 56-57 -0.2916890234042171 1103 58-59 -0.6213859681450344 1103 60-61 -0.661594894942219 1103 62-63 -0.5895482514513759 1103 64-65 -0.5226845055466853 1103 66-67 -0.29215806461022 1103 68-69 -0.4165814372555161 1103 70-71 -0.11259780807373687 1103 72-73 -0.14079875696853605 1103 74-75 -0.1266979306760092 1103 76-77 -0.06831402951435805 1103 78-79 -0.009716512600636662 1103 80-81 0.1733692606485171 1103 82-83 -0.046056635909135224 1103 84-85 0.05200947539887579 1103 86-87 0.18789956603025715 1103 88-89 0.04747612895249986 1103 90-91 0.39094402958044583 1103 92-93 0.34012092054471665 1103 94-95 0.30723917966994563 1103 96-97 0.5069316261884254 1103 98-99 0.674328314815579 1103 100-101 1.6026068129947006 1104 1 0.09320260942353542 1104 2 0.16375744057753394 1104 3 0.09401267558018844 1104 4 0.10090441287531604 1104 5 -0.11678542412040116 1104 6 0.09633938178669155 1104 7 0.1554968413368769 1104 8 0.09020658080955002 1104 9 0.1281222996689806 1104 10-11 0.11728768831167002 1104 12-13 0.15839927849768998 1104 14-15 0.20920316277389617 1104 16-17 0.07973783246916355 1104 18-19 0.017578213678476118 1104 20-21 0.10790813095018592 1104 22-23 0.3026884176782332 1104 24-25 0.03765914903729595 1104 26-27 0.09707490383657102 1104 28-29 0.0501339018012672 1104 30-31 -0.059323481589473204 1104 32-33 0.1537145918453362 1104 34-35 0.1814863160363771 1104 36-37 0.2731411332495881 1104 38-39 0.09301816707049682 1104 40-41 -0.02564103610983892 1104 42-43 0.1664854386767658 1104 44-45 0.030398492518621367 1104 46-47 0.13152122443830194 1104 48-49 0.3377759728952938 1104 50-51 0.3505718413284882 1104 52-53 0.35190471334262696 1104 54-55 0.35311144247577175 1104 56-57 0.2393689970735977 1104 58-59 0.21920170250343318 1104 60-61 0.3727667731465161 1104 62-63 0.37822851902644317 1104 64-65 0.27598230332703366 1104 66-67 0.3871614746389227 1104 68-69 0.4029231489219569 1104 70-71 0.45515496496380337 1104 72-73 0.48707293671746754 1104 74-75 0.43073594304412666 1104 76-77 0.31754881178256866 1104 78-79 0.6231599805734227 1104 80-81 0.6537606855631957 1104 82-83 0.9312364528280703 1104 84-85 0.7958821289824947 1104 86-87 0.92062674179817 1104 88-89 0.9673913379968688 1104 90-91 0.9712634621743028 1104 92-93 1.2084682968245808 1104 94-95 1.305506031205784 1104 96-97 1.7658666731167543 1104 98-99 1.9238830289794038 1104 100-101 2.805449176475932 1105 1 0.2273722868336776 1105 2 0.2390498277996045 1105 3 0.2002502696510433 1105 4 0.08338973996343668 1105 5 -0.012946904692185512 1105 6 0.12110125934820104 1105 7 0.24748278961396863 1105 8 0.22479752430277244 1105 9 0.15791794991817198 1105 10-11 0.23356783739702536 1105 12-13 0.2013719890236345 1105 14-15 0.37137632455435465 1105 16-17 0.31880633461833696 1105 18-19 0.46887200036075427 1105 20-21 0.34369575572348765 1105 22-23 0.43888945442120075 1105 24-25 0.371194785291884 1105 26-27 0.20995636384484584 1105 28-29 0.12049604783066314 1105 30-31 0.22224648428083071 1105 32-33 0.5184274882995581 1105 34-35 0.20601257019549024 1105 36-37 0.03656809713493914 1105 38-39 0.05791028011260835 1105 40-41 -0.10749874813519966 1105 42-43 -0.24514864531941072 1105 44-45 -0.287253984383149 1105 46-47 -0.0014310653927651629 1105 48-49 -0.011832035444349742 1105 50-51 -0.3063176441556834 1105 52-53 -0.14623171974746185 1105 54-55 -0.22096712721877765 1105 56-57 0.10182562094725256 1105 58-59 -0.23696520454671344 1105 60-61 -0.2130429859464087 1105 62-63 -0.09822699099112242 1105 64-65 -0.0884664093123817 1105 66-67 0.08387488518057751 1105 68-69 -0.032294695623718894 1105 70-71 0.12926280908107657 1105 72-73 0.29392600767581456 1105 74-75 0.10754631618591048 1105 76-77 -0.07072642491184666 1105 78-79 0.07103008781777476 1105 80-81 0.20690090290792895 1105 82-83 0.10578698333772607 1105 84-85 0.2763360967377899 1105 86-87 0.24885014134406092 1105 88-89 0.40042788104454985 1105 90-91 0.46458746577291166 1105 92-93 0.64776671029367 1105 94-95 0.6470992738122057 1105 96-97 0.6433496439708506 1105 98-99 0.9310588325980085 1105 100-101 1.6258220326599755 1106 1 -0.11928101884005571 1106 2 0.024963356390955482 1106 3 0.0649116153608773 1106 4 0.05851954083776434 1106 5 -0.15580990763394453 1106 6 -0.002517225702717951 1106 7 -0.08161370253937861 1106 8 0.02937542240762525 1106 9 -0.1384382494653451 1106 10-11 0.02595968953537664 1106 12-13 -0.21709665366356745 1106 14-15 -0.06902075477947278 1106 16-17 -0.07877403644222625 1106 18-19 -0.18721428352518643 1106 20-21 -0.007877090988415603 1106 22-23 -7.575340813161802E-4 1106 24-25 -0.042653180672751034 1106 26-27 0.07082037472117264 1106 28-29 -0.08882031469761387 1106 30-31 -0.07187116277799532 1106 32-33 -0.016149940998516854 1106 34-35 -0.16119205223925803 1106 36-37 0.1648893000931153 1106 38-39 -0.14748861584745043 1106 40-41 -0.15832619208348575 1106 42-43 0.275695675672587 1106 44-45 0.24711248186862633 1106 46-47 0.35561803039617246 1106 48-49 0.3467553325572439 1106 50-51 0.13754567523065475 1106 52-53 0.2638247714682649 1106 54-55 0.3734769265533586 1106 56-57 0.16111209704396856 1106 58-59 0.5003008315748545 1106 60-61 0.29503807074404875 1106 62-63 0.20543380387075416 1106 64-65 -0.03540334308170401 1106 66-67 0.1721013891712886 1106 68-69 -0.36107767324991613 1106 70-71 0.14468948184222796 1106 72-73 0.4528046218830042 1106 74-75 0.11371698529037388 1106 76-77 0.2501952491971302 1106 78-79 0.608162514010008 1106 80-81 0.425063063169528 1106 82-83 0.24245001675193123 1106 84-85 0.01882932834005402 1106 86-87 0.14487172989580088 1106 88-89 0.1351096198488584 1106 90-91 0.49902132299781243 1106 92-93 0.9568598035979399 1106 94-95 0.8940520298100019 1106 96-97 1.1181864406141955 1106 98-99 1.2882485704178244 1106 100-101 1.9130628704176722 1107 1 0.22272895588153574 1107 2 0.0737438441958389 1107 3 0.008410966320774094 1107 4 0.10276813458420264 1107 5 -0.08607794981016781 1107 6 0.007070349164621348 1107 7 0.12664846411590247 1107 8 -0.004579623025186663 1107 9 0.31399963625804617 1107 10-11 0.12736935909425284 1107 12-13 0.011001089498741123 1107 14-15 0.09509529814576467 1107 16-17 0.07369345461923871 1107 18-19 0.13434523459100944 1107 20-21 0.14066199944450375 1107 22-23 -0.03488337954144782 1107 24-25 0.28096732443986383 1107 26-27 0.25928112487488875 1107 28-29 0.12071715882838419 1107 30-31 0.1341706035220227 1107 32-33 0.023600918694953066 1107 34-35 0.041092113459541224 1107 36-37 0.12417057357811956 1107 38-39 -0.03614977136656705 1107 40-41 0.13374185464739696 1107 42-43 -0.08685584900232612 1107 44-45 -0.22053799295544962 1107 46-47 -0.27315380354065155 1107 48-49 -0.17744133798088768 1107 50-51 -0.3760044602707069 1107 52-53 -0.48561158844155017 1107 54-55 -0.41613159978400205 1107 56-57 -0.29155352204919893 1107 58-59 -0.18527909145127097 1107 60-61 -0.21357025063327484 1107 62-63 -0.10323600551641476 1107 64-65 -0.22425123996402618 1107 66-67 -0.34018482612783885 1107 68-69 -0.10647176584630813 1107 70-71 -0.11206850590571804 1107 72-73 0.1461274794813221 1107 74-75 -0.16730599300662874 1107 76-77 -0.0408707707067677 1107 78-79 0.08920371097660151 1107 80-81 0.05192193680027657 1107 82-83 0.0393346242534669 1107 84-85 -0.04613161538702926 1107 86-87 0.3337879467890623 1107 88-89 0.4682607664863774 1107 90-91 0.5850476034286807 1107 92-93 0.5138273060460712 1107 94-95 0.8459205821089704 1107 96-97 1.0386961078957384 1107 98-99 0.9104161366313015 1107 100-101 1.5883834051356231 1108 1 0.2336838959394143 1108 2 0.282025503283613 1108 3 0.20463530144547804 1108 4 0.16633893978378467 1108 5 0.02517095928909896 1108 6 0.18919335683538208 1108 7 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-0.15016359967539472 2316 58-59 -0.09074507831451939 2316 60-61 -0.1555435178235811 2316 62-63 -0.05960916246831971 2316 64-65 -0.015216285207706903 2316 66-67 -0.20979577647463543 2316 68-69 -0.43251099234026924 2316 70-71 -0.39737005383644686 2316 72-73 -0.03556835442616091 2316 74-75 -0.2610729306760078 2316 76-77 -0.23200576680249085 2316 78-79 -0.24078113124470235 2316 80-81 -0.496201713927757 2316 82-83 -0.162952822349812 2316 84-85 -0.24129031273671941 2316 86-87 0.09316969314889789 2316 88-89 0.002388734884700483 2316 90-91 0.1687644744956991 2316 92-93 0.09519242478200596 2316 94-95 0.020083459330962228 2316 96-97 -0.15820343737089715 2316 98-99 -0.06371458772678906 2316 100-101 -0.2598137378527561 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 3.0 6 23.0 7 21.0 8 82.0 9 172.0 10 297.0 11 531.0 12 981.0 13 1412.0 14 1875.0 15 2715.0 16 4009.0 17 6064.0 18 5622.0 19 3033.0 20 2282.0 21 2149.0 22 2168.0 23 2356.0 24 2630.0 25 3153.0 26 3787.0 27 4626.0 28 5809.0 29 7496.0 30 9432.0 31 12004.0 32 16155.0 33 22504.0 34 32576.0 35 49896.0 36 82623.0 37 138511.0 38 121605.0 39 11218.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.682543256738256 20.264633662517024 15.692161425104455 21.360661655640264 2 15.094083476533612 20.850913326021487 41.31974091764882 22.73526227979608 3 19.301670888246623 25.31715438121403 30.412702609043592 24.96847212149576 4 12.757090646669834 17.762431630053836 38.15552086198827 31.32495686128805 5 13.351377425233144 37.957444527816484 34.386858184156935 14.304319862793442 6 30.739592227529467 37.63374786985864 17.578801833439915 14.047858069171987 7 27.70702116029738 33.74947751249504 20.806970787143054 17.73653054006452 8 25.613498576333193 36.43354494821568 19.55568019834876 18.397276277102364 9 26.04041999063988 17.607203718324755 20.829120684789093 35.52325560624627 10-11 24.435981436956748 27.962906066571158 27.210613373536592 20.390499122935505 12-13 25.422230082235387 25.184286047315492 28.34544043315818 21.04804343729094 14-15 22.33058007723884 26.510658745101125 26.926505210584793 24.23225596707525 16-17 21.154491963516975 29.861098706374527 27.597432755411543 21.386976574696956 18-19 21.028112507189785 27.89234792487612 30.723515688915406 20.35602387901869 20-21 21.781317795047173 27.050408031845862 31.045431445558986 20.122842727547976 22-23 21.59081064232081 26.945642494973843 30.806337377181837 20.657209485523513 24-25 21.2612659281535 27.088333093672322 31.038184697361732 20.612216280812444 26-27 21.197492370199612 27.27194778956845 30.849580617761514 20.680979222470423 28-29 20.97594175145764 27.368847890705382 31.173098633817308 20.482111724019664 30-31 21.232825937645973 27.206537107049684 30.973733498029276 20.586903457275067 32-33 21.123321341426383 27.212578283811638 30.862399119720195 20.80170125504178 34-35 21.426632036611636 27.301356502602616 30.78755390106141 20.48445755972434 36-37 21.464699015668387 27.176787601047835 30.69139002342711 20.667123359856667 38-39 21.153777450689685 27.508386594310334 30.728427964603032 20.609407990396946 40-41 21.497279492410087 27.351104458202784 30.649474297187318 20.502141752199808 42-43 21.395260345161777 27.37712957099076 30.393565888633923 20.83404419521354 44-45 21.483525120484725 27.4932925108338 30.195580023650376 20.8276023450311 46-47 21.60347396136629 27.91449782252216 29.58556469735023 20.896463518761323 48-49 21.690912468606093 28.03775128522995 29.442126247271457 20.829209998892505 50-51 21.66385029527243 28.24308440897285 29.011900211138542 21.08116508461618 52-53 21.67019159661464 28.488430251044083 28.825233735008627 21.01614441733265 54-55 21.669209141477115 28.398223006598528 28.887842921500052 21.044724930424312 56-57 21.665190006823597 28.54916384136386 28.79567076677944 20.989975385033098 58-59 21.73967796906874 28.246210402592254 28.952774274680166 21.06133735365884 60-61 21.769866136021808 28.308462332670025 29.12872305840071 20.79294847290746 62-63 21.753432340994102 28.229240722944077 29.208033982230063 20.809292953831754 64-65 21.665190006823597 28.158950523559273 29.072991057871967 21.102868411745163 66-67 21.593202839473978 28.45181146864539 28.836219369728234 21.1187663221524 68-69 21.581949262444134 28.341151294518614 28.932946543722828 21.143952899314424 70-71 21.748877321720116 28.42465998120831 28.791115747505458 21.03534694956611 72-73 21.78035479197815 28.21866275112938 28.713463767882946 21.28751868900952 74-75 21.80431048880847 28.454031247515942 28.42920186023913 21.312456403436457 76-77 21.640717942488862 28.30819439035979 28.802190696328477 21.24889697082287 78-79 21.752449885856574 28.35633469209856 28.520404700065377 21.370810721979485 80-81 21.706383195357805 28.673514682805145 28.41432492321213 21.20577719862492 82-83 21.70533081109855 28.763301126610113 28.21776960849986 21.313598453791478 84-85 21.598204429265017 28.53174759119863 28.293278935090083 21.57676904444627 86-87 21.551046582663773 28.632315271639914 28.376698307676367 21.439939838019942 88-89 21.58543251247718 28.459581795642187 28.37116083326486 21.583824858615774 90-91 21.472077001610455 28.390235101079796 28.52359094323579 21.614096954073965 92-93 21.707225142861226 28.07269979582322 28.665669898125966 21.55440516318959 94-95 21.334281253684207 28.29256442226279 28.672774560485298 21.700379763567707 96-97 21.46208973566598 28.30917684549732 28.48771573821679 21.741017680619912 98-99 21.442070665571077 28.446464935157728 28.24925869029331 21.862205708977886 100-101 21.119695154794158 28.923850332151574 28.322431329883635 21.63402318317063 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 155.0 1 114.0 2 83.5 3 98.0 4 94.0 5 91.5 6 153.0 7 331.5 8 579.5 9 746.0 10 725.5 11 680.5 12 761.0 13 845.5 14 932.0 15 1014.0 16 1045.0 17 1082.5 18 1158.0 19 1206.0 20 1282.0 21 1401.0 22 1625.0 23 1945.5 24 2403.5 25 3066.5 26 3788.0 27 4658.0 28 5552.0 29 6560.0 30 7880.0 31 9117.5 32 10383.5 33 11881.5 34 13466.0 35 14944.0 36 16580.0 37 18012.0 38 18789.5 39 19264.0 40 19768.0 41 20456.5 42 20847.5 43 21114.5 44 21629.0 45 22096.0 46 21797.0 47 21480.5 48 21724.0 49 21007.0 50 19703.0 51 18346.5 52 17056.5 53 16163.0 54 15095.5 55 13748.0 56 11854.5 57 10133.5 58 9043.0 59 7824.5 60 6284.5 61 4879.0 62 3722.5 63 2706.0 64 1917.0 65 1353.5 66 974.5 67 736.0 68 557.5 69 390.0 70 277.0 71 185.5 72 139.0 73 124.0 74 82.5 75 51.0 76 42.0 77 35.0 78 23.0 79 11.5 80 10.0 81 8.0 82 3.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04572882094665805 2 0.0 3 0.0 4 0.0 5 0.014647512959476406 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004733647480806399 14-15 0.0 16-17 0.0 18-19 0.0 20-21 8.93141034114415E-5 22-23 8.93141034114415E-5 24-25 6.251987238800904E-4 26-27 0.0018755961716402714 28-29 0.0025007948955203616 30-31 4.4657051705720746E-4 32-33 0.0 34-35 0.0 36-37 8.93141034114415E-5 38-39 0.0 40-41 0.0 42-43 0.009913865478670006 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.78628206822883E-4 74-75 2.6794231023432447E-4 76-77 0.0 78-79 0.0 80-81 8.93141034114415E-5 82-83 1.78628206822883E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.007413070583149644 92-93 0.002322166688697479 94-95 0.0 96-97 0.0 98-99 3.57256413645766E-4 100-101 0.011164262926430187 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 559822.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.31844170270915 #Duplication Level Percentage of deduplicated Percentage of total 1 85.42347306247623 61.77692456711633 2 9.350609576588177 13.524430270985718 3 2.3773315422425516 5.157747376370388 4 0.9493065053520048 2.7460946866120604 5 0.5489913195419873 1.985109836879529 6 0.31741236539257905 1.3772860585417346 7 0.201109307739652 1.018073822334955 8 0.1449720872812039 0.8387324354052643 9 0.10525854036381092 0.6850920253511267 >10 0.5440930404213254 7.088586775580925 >50 0.027457951926657034 1.3452322911143175 >100 0.008986230606416387 1.025350750906174 >500 2.496175168438581E-4 0.12528053188854213 >1k 7.488525505315744E-4 1.3060585709129717 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3075 0.5492817359803652 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2553 0.45603781201882027 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1607 0.2870555283643729 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 694 0.1239679755350808 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 1.78628206822883E-4 0.0 0.0 0.0 12-13 0.0 1.78628206822883E-4 0.0 0.0 0.0 14-15 0.0 1.78628206822883E-4 0.0 0.0 0.0 16-17 0.0 1.78628206822883E-4 0.0 0.0 0.0 18-19 8.93141034114415E-5 1.78628206822883E-4 0.0 8.93141034114415E-5 0.0 20-21 1.78628206822883E-4 1.78628206822883E-4 0.0 1.78628206822883E-4 0.0 22-23 1.78628206822883E-4 1.78628206822883E-4 0.0 1.78628206822883E-4 0.0 24-25 1.78628206822883E-4 1.78628206822883E-4 0.0 3.57256413645766E-4 0.0 26-27 1.78628206822883E-4 1.78628206822883E-4 0.0 3.57256413645766E-4 0.0 28-29 1.78628206822883E-4 1.78628206822883E-4 0.0 8.038269307029735E-4 0.0 30-31 1.78628206822883E-4 1.78628206822883E-4 0.0 0.0038405064466919842 0.0 32-33 1.78628206822883E-4 1.78628206822883E-4 0.0 0.0065199295490352294 0.0 34-35 2.679423102343245E-4 1.78628206822883E-4 0.0 0.010003179582081446 0.0 36-37 3.57256413645766E-4 1.78628206822883E-4 0.0 0.01554065399359082 0.0 38-39 3.57256413645766E-4 1.78628206822883E-4 0.0 0.024829320748380734 0.0 40-41 3.57256413645766E-4 1.78628206822883E-4 0.0 0.03170650671106173 0.0 42-43 4.4657051705720746E-4 1.78628206822883E-4 0.0 0.04019134653514868 0.0 44-45 5.358846204686489E-4 1.78628206822883E-4 0.0 0.046443333773949574 0.0 46-47 5.358846204686489E-4 1.78628206822883E-4 0.0 0.05528543001168229 0.0 48-49 7.14512827291532E-4 1.78628206822883E-4 0.0 0.061716045457306065 0.0 50-51 7.14512827291532E-4 1.78628206822883E-4 0.0 0.06770009038587266 0.0 52-53 7.14512827291532E-4 1.78628206822883E-4 0.0 0.08395525720675501 0.0 54-55 7.14512827291532E-4 1.78628206822883E-4 0.0 0.1117319433677133 0.0 56-57 0.0010717692409372979 1.78628206822883E-4 0.0 0.15790733483142855 0.0 58-59 0.0010717692409372979 1.78628206822883E-4 0.0 0.2161401302556884 0.0 60-61 0.0010717692409372979 1.78628206822883E-4 0.0 0.2899135796735391 0.0 62-63 0.0010717692409372979 1.78628206822883E-4 0.0 0.33055149672574496 0.0 64-65 0.0010717692409372979 1.78628206822883E-4 0.0 0.3843185869794328 0.0 66-67 0.0010717692409372979 1.78628206822883E-4 0.0 0.47997399173308664 0.0 68-69 0.0010717692409372979 1.78628206822883E-4 0.0 0.610551210920614 0.0 70-71 0.0010717692409372979 1.78628206822883E-4 0.0 0.7510422955868115 0.0 72-73 0.0010717692409372979 1.78628206822883E-4 0.0 0.9235971433777165 0.0 74-75 0.0011610833443487394 1.78628206822883E-4 0.0 1.0944550232038042 0.0 76-77 0.0012503974477601808 1.78628206822883E-4 0.0 1.2563814926887473 0.0 78-79 0.0012503974477601808 1.78628206822883E-4 0.0 1.398122974802705 0.0 80-81 0.0012503974477601808 1.78628206822883E-4 0.0 1.5008341937258627 0.0 82-83 0.0012503974477601808 1.78628206822883E-4 0.0 1.6602598683152858 0.0 84-85 0.0012503974477601808 1.78628206822883E-4 0.0 1.9746455123235598 0.0 86-87 0.0012503974477601808 1.78628206822883E-4 0.0 2.374951323813641 0.0 88-89 0.0012503974477601808 1.78628206822883E-4 0.0 2.815001911321813 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTACG 25 0.005012632 57.020966 1 GGTATCA 1810 0.0 42.26692 1 GTATCAA 2930 0.0 35.354298 1 CGTACAC 65 0.0058412505 29.23107 3 ATCAACG 3485 0.0 29.031864 3 TCAACGC 3540 0.0 28.580803 4 TATCAAC 3605 0.0 28.329004 2 CAACGCA 3610 0.0 28.026606 5 AACGCAG 3710 0.0 27.396759 6 GCGTTAC 35 0.008391055 27.143139 12-13 GTCGATT 45 8.9328724E-4 26.396235 40-41 GTACATG 2375 0.0 25.009195 1 TACATGG 2450 0.0 24.041065 2 ACGCAGA 4185 0.0 23.948874 7 CGCAGAG 4190 0.0 23.920294 8 TGGTATC 1000 0.0 23.750246 2 GTGGTAT 1035 0.0 23.414408 1 ACATGGG 2480 0.0 23.367176 3 GAGTACT 2655 0.0 23.347698 12-13 GCAGAGT 4395 0.0 22.588402 9 >>END_MODULE