FastQCFastQC Report
Thu 26 May 2016
SRR937711_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937711_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836651
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39810.47582564295028634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30290.36203865171977323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18890.22578112020424285No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10670.12753226853251834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29900.033.401021
GTATCAA44200.028.835281
CGATATA350.00837448527.1549146-47
TCAACGC49450.025.479284
ATCAACG49300.025.4588383
GTATAGC6000.025.363571
CAACGCA50100.025.1487085
TATCAAC50650.024.9679952
GTACATG32050.024.9283121
AACGCAG51550.024.5115286
TACATGG31950.024.1060282
ACATGGG32250.023.1446933
ACGCAGA56800.022.2459377
CGCAGAG56400.022.1497128
GAGTACT33900.022.06979412-13
ATAGCCC6300.021.8846193
TAGCCCT9650.021.6786844
CATGGGG15300.020.8204884
GCAGAGT60450.020.6657359
GTACTTT36250.020.50679214-15
Produced by FastQC (version 0.11.5)