##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937711_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 836651 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.936760967237234 33.0 31.0 34.0 30.0 34.0 2 32.092135191376094 33.0 31.0 34.0 30.0 34.0 3 32.116895814383774 34.0 31.0 34.0 30.0 34.0 4 35.70015454472654 37.0 35.0 37.0 35.0 37.0 5 35.69886726962616 37.0 35.0 37.0 35.0 37.0 6 35.76679882053568 37.0 35.0 37.0 35.0 37.0 7 35.722011926119734 37.0 35.0 37.0 35.0 37.0 8 35.71572973677196 37.0 35.0 37.0 35.0 37.0 9 37.39580541946403 39.0 37.0 39.0 35.0 39.0 10-11 37.27886478352383 39.0 37.0 39.0 34.0 39.0 12-13 37.18691963554696 39.0 37.0 39.0 34.0 39.0 14-15 38.39040890407111 40.0 38.0 41.0 34.0 41.0 16-17 38.310027717650485 40.0 38.0 41.0 34.0 41.0 18-19 38.27066662204432 40.0 38.0 41.0 34.0 41.0 20-21 38.19806346971437 40.0 38.0 41.0 34.0 41.0 22-23 38.11861218118427 40.0 38.0 41.0 34.0 41.0 24-25 38.17219844355651 40.0 38.0 41.0 34.0 41.0 26-27 38.09725680122297 40.0 38.0 41.0 34.0 41.0 28-29 37.9828596392044 40.0 38.0 41.0 33.0 41.0 30-31 37.76596932293154 40.0 37.5 41.0 33.0 41.0 32-33 37.618024719984795 40.0 37.0 41.0 33.0 41.0 34-35 37.62729023212785 40.0 37.0 41.0 33.0 41.0 36-37 37.504832361402784 40.0 37.0 41.0 33.0 41.0 38-39 37.36661821954435 40.0 37.0 41.0 33.0 41.0 40-41 37.1509584044004 40.0 37.0 41.0 31.5 41.0 42-43 36.89425459361191 40.0 36.0 41.0 31.0 41.0 44-45 36.63263475451532 40.0 36.0 41.0 30.5 41.0 46-47 36.259908850882866 39.0 36.0 41.0 30.0 41.0 48-49 35.931231780037315 39.0 36.0 41.0 29.0 41.0 50-51 35.06627136045974 38.5 34.5 40.0 27.0 40.5 52-53 35.16242435615328 38.5 35.0 40.0 27.5 40.5 54-55 35.4663151062988 39.0 35.0 40.0 27.0 41.0 56-57 35.27075387467415 39.0 35.0 40.0 26.5 41.0 58-59 35.06311114192178 39.0 35.0 40.0 26.0 41.0 60-61 34.79173394880303 38.5 34.5 40.0 25.5 41.0 62-63 34.467301180540034 38.0 34.0 40.0 24.0 41.0 64-65 34.138625304935985 38.0 34.0 40.0 23.0 41.0 66-67 33.74365834738738 37.5 33.5 40.0 22.0 41.0 68-69 33.28174412030823 37.0 33.0 39.5 20.5 41.0 70-71 32.86053145218257 36.0 32.5 39.0 18.5 40.5 72-73 32.416804617456975 36.0 32.0 39.0 17.5 40.0 74-75 31.896131720394763 35.0 31.0 38.0 13.5 40.0 76-77 31.410238558251887 35.0 31.0 37.5 9.0 39.0 78-79 30.838728454277827 35.0 30.5 37.0 7.0 39.0 80-81 30.265671110176164 34.0 30.0 36.0 2.0 39.0 82-83 29.750692343641497 34.0 29.0 36.0 2.0 37.5 84-85 29.21132467420705 34.0 29.0 35.5 2.0 37.0 86-87 28.66952767641466 34.0 29.0 35.0 2.0 36.5 88-89 28.206353067168987 34.0 27.5 35.0 2.0 36.0 90-91 27.65918644691753 33.5 26.5 35.0 2.0 36.0 92-93 27.062625276250195 33.0 26.0 35.0 2.0 35.0 94-95 26.345789941086547 32.0 25.0 34.5 2.0 35.0 96-97 25.56006865467202 32.0 22.0 34.0 2.0 35.0 98-99 24.542429878169035 32.0 10.0 34.0 2.0 35.0 100-101 21.707566237296078 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34265261980644013 1101 2 0.4041097297245031 1101 3 0.2711453278520537 1101 4 0.21257907396107356 1101 5 0.16288152135830813 1101 6 0.21801741899677296 1101 7 0.2631765018143142 1101 8 0.2851528071466305 1101 9 0.16516618520468285 1101 10-11 0.35723925840149917 1101 12-13 0.17339716155368023 1101 14-15 0.37873212145074575 1101 16-17 0.30713075951997126 1101 18-19 0.46860079064703797 1101 20-21 0.3801247858144734 1101 22-23 0.4208323755464676 1101 24-25 0.2559424162968824 1101 26-27 0.3008916636495087 1101 28-29 0.2509183611628103 1101 30-31 0.4237847163684094 1101 32-33 0.3430398099578369 1101 34-35 0.497287980528931 1101 36-37 0.5345591012409514 1101 38-39 0.4283933566099307 1101 40-41 0.48986649888568223 1101 42-43 0.4622316874926824 1101 44-45 0.45822313828873007 1101 46-47 0.24832009862267057 1101 48-49 0.2009659476852761 1101 50-51 0.07956645700422627 1101 52-53 0.042562393830877454 1101 54-55 0.24329144448893203 1101 56-57 0.024200182142756432 1101 58-59 -0.0683748327044853 1101 60-61 0.3823637082727416 1101 62-63 0.32146947040893536 1101 64-65 0.2897572955699701 1101 66-67 0.13719261291139162 1101 68-69 0.0977037827959748 1101 70-71 0.3139839991713771 1101 72-73 0.18536610513691443 1101 74-75 0.22064762350676048 1101 76-77 0.3162365301283714 1101 78-79 0.4892442795799745 1101 80-81 0.4408736678052101 1101 82-83 0.2470996813095354 1101 84-85 0.4423476256260379 1101 86-87 0.5164473015076432 1101 88-89 0.4253731098215816 1101 90-91 0.6788117115169179 1101 92-93 0.6813984411594696 1101 94-95 0.6646747838727656 1101 96-97 0.9925641652199744 1101 98-99 1.128708587792179 1101 100-101 1.1581279714360626 1102 1 0.02951747908509006 1102 2 0.12972893810233188 1102 3 0.21169902702644094 1102 4 -0.024184162802157516 1102 5 -0.03784878282026938 1102 6 -0.05397495644388073 1102 7 0.027699335302663997 1102 8 -0.028476092689167842 1102 9 0.029393911501379932 1102 10-11 -0.07453012164374684 1102 12-13 0.17085946936080632 1102 14-15 0.17082508595750312 1102 16-17 0.19552397549939826 1102 18-19 0.13623775138744065 1102 20-21 0.29098978288636346 1102 22-23 0.3938363830332321 1102 24-25 0.40387494353975484 1102 26-27 0.21536799191894573 1102 28-29 0.17285274861443867 1102 30-31 0.23893508324292156 1102 32-33 0.3633528302708626 1102 34-35 0.48106856775779505 1102 36-37 0.5624048187418325 1102 38-39 0.5274954039878423 1102 40-41 0.3698772926895799 1102 42-43 0.31939751017574025 1102 44-45 0.4214093773370351 1102 46-47 0.7369177079099316 1102 48-49 0.5901112851064738 1102 50-51 0.6821305595683285 1102 52-53 0.4685501991290266 1102 54-55 0.3965806839850714 1102 56-57 0.3269663814606787 1102 58-59 0.3757901976674418 1102 60-61 0.4746564729792908 1102 62-63 0.456231260343138 1102 64-65 0.27726978308245975 1102 66-67 0.10026976564371637 1102 68-69 0.04481988508448609 1102 70-71 0.20882479056388803 1102 72-73 0.012515966769534259 1102 74-75 0.001613783783266598 1102 76-77 0.033892437439455136 1102 78-79 0.5535076024639842 1102 80-81 0.40411157931898245 1102 82-83 0.33865754872947207 1102 84-85 0.4460795259096635 1102 86-87 0.7115338241803748 1102 88-89 0.5397644655922456 1102 90-91 0.6164085973896682 1102 92-93 0.6910037783510141 1102 94-95 0.8609210663259468 1102 96-97 0.9297361885988948 1102 98-99 0.9940673669440621 1102 100-101 1.0667366041826263 1103 1 0.23485607819455012 1103 2 0.26901012410209546 1103 3 0.20524719187605456 1103 4 0.14664723049864392 1103 5 0.1918025447175964 1103 6 0.19691653248987961 1103 7 0.25016541205127396 1103 8 0.30362450971176713 1103 9 0.2680751412838305 1103 10-11 0.23767880402457564 1103 12-13 0.2935137894567319 1103 14-15 0.36742325975256307 1103 16-17 0.4219721657963049 1103 18-19 0.5160103703146817 1103 20-21 0.33704661815343684 1103 22-23 0.40640557135324684 1103 24-25 0.35138516413117316 1103 26-27 0.3971939523878376 1103 28-29 0.3998548899658587 1103 30-31 0.40907139854051877 1103 32-33 0.35539360373544326 1103 34-35 0.4050811513921744 1103 36-37 0.07888444589999466 1103 38-39 0.32725404356850873 1103 40-41 0.41062019828231655 1103 42-43 0.2339645893701814 1103 44-45 0.14563676285474259 1103 46-47 0.16337723281216654 1103 48-49 0.18625262985738544 1103 50-51 0.15104318198483924 1103 52-53 0.11344923779892468 1103 54-55 0.15003968327276596 1103 56-57 7.903328152210065E-4 1103 58-59 0.17479097442127767 1103 60-61 0.10682186042833308 1103 62-63 0.23177384901142517 1103 64-65 0.2973414800173302 1103 66-67 0.20814687185230696 1103 68-69 0.18197249887781197 1103 70-71 0.10838518567489785 1103 72-73 0.2453148697575358 1103 74-75 -0.004939729444327412 1103 76-77 0.16509216096398305 1103 78-79 0.3974250865371971 1103 80-81 0.3569251728444769 1103 82-83 0.5015069351606165 1103 84-85 0.7528778443876227 1103 86-87 0.4114484812696695 1103 88-89 0.6341572807859528 1103 90-91 0.9637748018192731 1103 92-93 0.5245406530447276 1103 94-95 0.7767016150319677 1103 96-97 0.8946439171328748 1103 98-99 0.7173929845967066 1103 100-101 1.0419382657024236 1104 1 0.1244225739901843 1104 2 0.08677189514529005 1104 3 0.20137601670342775 1104 4 0.1554695168515181 1104 5 0.24370299873151424 1104 6 0.25221884974993003 1104 7 0.327399635002962 1104 8 0.30918624497316927 1104 9 0.25350313561060034 1104 10-11 0.24515019803657623 1104 12-13 0.3668301653315069 1104 14-15 0.38486104999346793 1104 16-17 0.47640975638517347 1104 18-19 0.42628104143074097 1104 20-21 0.5891915810881656 1104 22-23 0.6143658535627026 1104 24-25 0.4110344506992547 1104 26-27 0.3814519080028589 1104 28-29 0.5043545801162637 1104 30-31 0.5197376140454466 1104 32-33 0.5691470360650683 1104 34-35 0.46600030268952963 1104 36-37 0.42853868487570423 1104 38-39 0.4682213447137755 1104 40-41 0.4509628737751612 1104 42-43 0.30657699735522925 1104 44-45 0.4779361838638394 1104 46-47 0.30826302925525084 1104 48-49 0.41328810828329665 1104 50-51 0.3645314919692595 1104 52-53 0.3095425401213632 1104 54-55 0.45235823976262424 1104 56-57 0.5403363448306422 1104 58-59 0.3009483728256157 1104 60-61 0.1647164130392369 1104 62-63 0.283570587682469 1104 64-65 0.4220416778543523 1104 66-67 0.4344355998095537 1104 68-69 0.32262541289001234 1104 70-71 0.3819849637240651 1104 72-73 0.3835615878151515 1104 74-75 0.8751569073263887 1104 76-77 0.5983280018750179 1104 78-79 0.40490620386258414 1104 80-81 0.3717272969346972 1104 82-83 0.5238057338776549 1104 84-85 0.41011548994562474 1104 86-87 0.29178690443345445 1104 88-89 0.4024850783128606 1104 90-91 0.2483599793410498 1104 92-93 0.0651296524768874 1104 94-95 0.39122409662897795 1104 96-97 0.5641018229645312 1104 98-99 0.6865415594182522 1104 100-101 0.9565967440427663 1105 1 0.18426865574348383 1105 2 0.352668369804654 1105 3 0.15890130928026736 1105 4 0.23518919579800013 1105 5 0.21833832903694628 1105 6 0.29014870340142807 1105 7 0.2215583113781321 1105 8 0.2705264511587373 1105 9 0.3099458338058767 1105 10-11 0.3352707733375624 1105 12-13 0.41428228365990805 1105 14-15 0.3750068577939132 1105 16-17 0.39442365829083315 1105 18-19 0.404588601568193 1105 20-21 0.485269729743635 1105 22-23 0.5581965074449045 1105 24-25 0.370763081175312 1105 26-27 0.28376608866755504 1105 28-29 0.3122030889957088 1105 30-31 0.29805959275402216 1105 32-33 0.2512716125195382 1105 34-35 0.2398825630408581 1105 36-37 0.36884580044055326 1105 38-39 0.3521893955632791 1105 40-41 0.37755582549697664 1105 42-43 0.3891248189494405 1105 44-45 0.32455262584111466 1105 46-47 0.41200615039528543 1105 48-49 0.34139350803818047 1105 50-51 0.3044180658764546 1105 52-53 0.09572264009012343 1105 54-55 0.16443138998010198 1105 56-57 0.021784449990093435 1105 58-59 0.13127182943772908 1105 60-61 0.22347209035161342 1105 62-63 0.01183158785067917 1105 64-65 -0.05320193592019251 1105 66-67 0.06441901845276732 1105 68-69 0.21681863375849275 1105 70-71 0.09452671750293007 1105 72-73 0.22321055568576753 1105 74-75 0.32929834149360104 1105 76-77 0.2773976435735257 1105 78-79 0.26631219477888735 1105 80-81 0.1186528370303428 1105 82-83 0.34029186801842926 1105 84-85 0.10240065323594294 1105 86-87 0.15499371480521162 1105 88-89 0.2387652071187638 1105 90-91 0.4212776973618624 1105 92-93 0.5560819990993373 1105 94-95 0.795104794968438 1105 96-97 0.535990115084779 1105 98-99 0.7877389015949738 1105 100-101 1.0378544039086783 1106 1 0.29895937739297196 1106 2 0.26761682341290083 1106 3 0.15546488316343243 1106 4 0.13682149820350276 1106 5 0.19084692010223847 1106 6 0.21239956250796155 1106 7 0.11889508113758751 1106 8 0.3624508497841674 1106 9 0.23520356576464252 1106 10-11 0.36684293828601966 1106 12-13 0.04014139018912033 1106 14-15 0.1771975794433942 1106 16-17 0.18415733526677514 1106 18-19 0.07490647098400416 1106 20-21 0.213792066513399 1106 22-23 0.3936776071012531 1106 24-25 0.07243833890726847 1106 26-27 0.22620595121050968 1106 28-29 0.3457082436348813 1106 30-31 0.20720907729010918 1106 32-33 0.1464606159559665 1106 34-35 0.3251488860649161 1106 36-37 0.014893910406186706 1106 38-39 -0.052235003062158114 1106 40-41 -0.1591709786267259 1106 42-43 0.15738083269514647 1106 44-45 -0.0431449686606058 1106 46-47 -0.09465192520609378 1106 48-49 -0.15259274625734776 1106 50-51 0.044967894055147895 1106 52-53 0.021195148681194098 1106 54-55 0.06992967176704923 1106 56-57 0.0836401200931789 1106 58-59 0.12010344455800492 1106 60-61 0.07570576732756251 1106 62-63 0.22016492118401487 1106 64-65 -0.0018703207999166693 1106 66-67 -0.134034589794652 1106 68-69 -0.22884287826899907 1106 70-71 -0.03777568799534947 1106 72-73 0.28012636324590545 1106 74-75 0.3840663827307118 1106 76-77 0.496899361786582 1106 78-79 0.25406992673764606 1106 80-81 0.3336442459650506 1106 82-83 0.15622829413527128 1106 84-85 -0.04171446703897175 1106 86-87 0.07982189349936775 1106 88-89 0.2666861693180742 1106 90-91 0.48957666643402575 1106 92-93 0.39285175854950793 1106 94-95 0.43664259565572294 1106 96-97 1.0297615927480095 1106 98-99 1.1481340905984112 1106 100-101 1.4566688368983662 1107 1 0.18200511396169716 1107 2 0.2182254339495202 1107 3 0.3125537618781138 1107 4 0.2713288999390784 1107 5 0.3008125558410697 1107 6 0.3036490428340244 1107 7 0.27252648365201537 1107 8 0.2693700122013496 1107 9 0.32471115168844733 1107 10-11 0.318785597858799 1107 12-13 0.36462053218771473 1107 14-15 0.36094507316784075 1107 16-17 0.3756394190145329 1107 18-19 0.44644439007800685 1107 20-21 0.7000516245668891 1107 22-23 0.5872318084347796 1107 24-25 0.5230153743187742 1107 26-27 0.545677804617732 1107 28-29 0.4288968576447658 1107 30-31 0.5181214405303507 1107 32-33 0.5834221942912237 1107 34-35 0.6403212599354475 1107 36-37 0.6034174049305321 1107 38-39 0.6912657836847131 1107 40-41 0.743980218859889 1107 42-43 0.6877297904528916 1107 44-45 0.549430666920351 1107 46-47 0.4288781211567141 1107 48-49 0.2019932775397706 1107 50-51 0.20163708673182867 1107 52-53 -0.03281673409089336 1107 54-55 0.40399975801975785 1107 56-57 0.2888192299904091 1107 58-59 0.4052018273853477 1107 60-61 0.3963182721296121 1107 62-63 0.6140856570949254 1107 64-65 0.47797948685494873 1107 66-67 0.364248512777138 1107 68-69 0.3643326846891668 1107 70-71 0.06549338995836962 1107 72-73 0.21728133922250237 1107 74-75 0.04442854941579455 1107 76-77 0.32251652391958885 1107 78-79 0.4724829609492964 1107 80-81 0.5111385492862262 1107 82-83 0.6711764846174724 1107 84-85 0.42203465806693785 1107 86-87 0.3325544574521544 1107 88-89 0.4846189985019116 1107 90-91 0.7149914846402403 1107 92-93 0.8100723055359254 1107 94-95 1.1481590962898558 1107 96-97 1.1731573922038656 1107 98-99 1.6755505086109714 1107 100-101 2.049843084226321 1108 1 0.268770264516224 1108 2 0.06322060567043764 1108 3 0.09665165401728615 1108 4 0.24934803748163148 1108 5 0.26418835201941704 1108 6 0.15511849787250753 1108 7 0.13160657456022307 1108 8 0.14517350611966862 1108 9 0.11065500709368337 1108 10-11 0.238017999025395 1108 12-13 0.34807636074967263 1108 14-15 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>>Per sequence quality scores pass #Quality Count 2 2674.0 3 366.0 4 344.0 5 370.0 6 473.0 7 527.0 8 690.0 9 830.0 10 1166.0 11 1668.0 12 2493.0 13 3381.0 14 4033.0 15 5397.0 16 7396.0 17 9702.0 18 8201.0 19 5473.0 20 4908.0 21 4848.0 22 5314.0 23 5837.0 24 6653.0 25 7585.0 26 8807.0 27 10397.0 28 12451.0 29 15094.0 30 18964.0 31 23672.0 32 30464.0 33 40085.0 34 55270.0 35 77688.0 36 116133.0 37 171190.0 38 148852.0 39 17253.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.87346251780242 20.349311160022825 15.28023535166084 22.49699097051391 2 14.203586700159153 20.72155855113037 42.688898582961016 22.38595616574946 3 18.17118505540229 25.351312613698735 31.19461867930905 25.282883651589927 4 11.48885426653883 17.568552253116014 40.25587248322148 30.686720997123686 5 12.351677749385138 37.89402779708797 36.28124056131132 13.47305389221557 6 29.508061271410508 37.62872648197111 19.321425834387092 13.541786412231291 7 26.255399099262 33.430408349728715 22.84080934698291 17.473383204026376 8 24.118015464442202 35.701857433506134 21.814395558732908 18.36573154331876 9 25.12118193095983 16.782859222998702 22.751668047725563 35.34429079831591 10-11 23.08506644328295 27.350214451960426 29.69286934507575 19.871849759680877 12-13 24.275506141351364 25.124967444700896 29.64283469092673 20.956691723021013 14-15 21.232577758325768 26.78500329599864 28.60244269123528 23.37997625444032 16-17 19.995329676909442 29.64152276483127 29.534464589370824 20.82868296888846 18-19 19.526615527939896 28.359002483493274 32.24877082649794 19.86561116206889 20-21 20.999328863480297 27.27352734626873 32.10678793822191 19.620355852029064 22-23 20.08369528450709 27.79341992223163 31.768287389730865 20.35459740353041 24-25 19.819863508650297 27.50763490629466 32.39601051075592 20.27649107429912 26-27 20.029945658388407 27.716676474937422 32.15632088670759 20.09705697996658 28-29 19.610053772679457 28.114471562628918 32.10302608283303 20.17244858185859 30-31 20.118401655491542 27.73726736116517 32.135026028724404 20.009304954618887 32-33 19.68041216124779 27.742142936696794 32.126745332108605 20.450699569946813 34-35 20.155776315844623 27.915252319190557 31.984681037574692 19.944290327390128 36-37 19.975874547647766 27.839155669032316 31.83583916764196 20.34913061567795 38-39 19.85611863926879 28.054470911553906 31.947277023099673 20.14213342607763 40-41 20.422045754548964 27.867711105191162 31.660016702236245 20.05022643802363 42-43 19.962952408093475 27.973685912096386 31.545892794279297 20.517468885530842 44-45 20.39213479244325 28.054856721361336 31.21553643124769 20.337472054947725 46-47 20.31871555809949 28.12925846184059 30.72385169715795 20.82817428290197 48-49 20.321935564988834 28.190227576173054 30.85959250633154 20.628244352506574 50-51 20.462367316830164 28.637410905885957 30.33959945968731 20.560622317596568 52-53 20.603455658609533 28.6450395051333 30.16457827154786 20.58692656470931 54-55 20.595535578515317 28.35397356980104 30.214169144173535 20.83632170751011 56-57 20.720761705912846 28.61820484793777 29.822287775520394 20.838745670628995 58-59 20.690065935776218 28.624626997452957 29.95926527851356 20.726041788257266 60-61 20.772408723821474 28.378816791608507 30.169922496953273 20.67885198761675 62-63 20.57263435147633 28.37257748312656 30.29945675760614 20.755331407790965 64-65 20.52016633734012 28.479493062593193 30.100007123270288 20.900333476796394 66-67 20.431444355123034 28.413386399428713 30.112694620742825 21.042474624705424 68-69 20.60899392981574 28.377991690911486 30.098775187672857 20.91423919159992 70-71 20.509374711922604 28.138981106911583 30.193683787093896 21.15796039407192 72-73 20.469210914454276 27.969725702026587 30.375938589434163 21.185124794084974 74-75 20.522952266134467 28.18352474688038 29.955332183215866 21.33819080376929 76-77 20.33863909823946 28.104291503792254 30.092367001694093 21.46470239627419 78-79 20.28511975173246 28.0922694162552 30.041737106514628 21.58087372549771 80-81 20.229815977214745 28.269005371870648 29.94054666894934 21.560631981965265 82-83 20.347338989229534 28.32950334223839 30.051652502982485 21.271505165549595 84-85 20.452849071658203 28.15977091784299 29.941574233524694 21.445805776974105 86-87 20.184004611188357 28.16110907938438 29.8649264179075 21.789959891519757 88-89 20.485938815907218 27.79441455278533 29.819059858271835 21.900586773035617 90-91 20.35361731344463 27.666900320430898 30.155163259332333 21.824319106792142 92-93 20.211398192119884 27.49128202427027 30.532846986208064 21.764472797401783 94-95 19.978055762068433 27.778735518658586 30.291189143049372 21.952019576223602 96-97 19.981180493078266 27.682153851863422 30.3762644356213 21.960401219437006 98-99 20.029426253893025 27.506903138181883 30.429116907106774 22.03455370081832 100-101 20.084877226665647 27.152214037599258 30.75791018407023 22.00499855166487 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 935.0 1 520.5 2 93.5 3 75.0 4 69.5 5 71.0 6 89.0 7 156.5 8 281.5 9 354.5 10 383.0 11 395.0 12 435.0 13 539.0 14 617.0 15 706.0 16 846.0 17 972.5 18 1128.5 19 1358.0 20 1608.0 21 1943.0 22 2458.5 23 3169.0 24 4090.5 25 5259.0 26 6751.5 27 8604.5 28 10578.0 29 12667.5 30 15054.5 31 17798.5 32 20978.5 33 23881.0 34 26561.5 35 29145.5 36 31200.0 37 32999.5 38 33771.5 39 33654.5 40 33478.5 41 33247.5 42 33030.0 43 32635.0 44 32122.5 45 31703.0 46 31356.5 47 31281.0 48 31808.5 49 30530.0 50 27447.5 51 24764.0 52 22083.0 53 19219.0 54 16686.5 55 14590.0 56 12524.5 57 10537.5 58 8576.5 59 6779.5 60 5320.5 61 4136.5 62 3080.0 63 2145.5 64 1450.5 65 1074.0 66 862.0 67 641.5 68 459.0 69 333.5 70 229.0 71 175.0 72 152.0 73 127.0 74 96.0 75 67.5 76 52.5 77 34.0 78 20.5 79 16.5 80 11.5 81 7.0 82 7.5 83 5.5 84 4.0 85 2.5 86 1.5 87 1.5 88 2.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29821275537828795 2 0.26713647626071085 3 0.2640288483489532 4 0.26904886266794636 5 0.27717650489869733 6 0.18490386074958376 7 0.18621862640455816 8 0.18813101281179367 9 0.18442576414777487 10-11 0.1930912650555608 12-13 0.1832902847184788 14-15 0.18496362282480985 16-17 0.1901629233694814 18-19 0.18370861924506157 20-21 0.17934598775355554 22-23 0.17671645644360673 24-25 0.18006313265626886 26-27 0.1758797873904412 28-29 0.18663696093114093 30-31 0.19267293052897802 32-33 0.19578055844073575 34-35 0.19213507185194303 36-37 0.17133786967325684 38-39 0.16488356554883699 40-41 0.17133786967325684 42-43 0.1803619430323994 44-45 0.18287195019189603 46-47 0.1851429090504882 48-49 0.18478433659913154 50-51 0.18872863356405478 52-53 0.21030274272068042 54-55 0.17570050116476285 56-57 0.16350903781863643 58-59 0.16356879989386255 60-61 0.15884759595099987 62-63 0.16595928290290696 64-65 0.1626126066902448 66-67 0.1602221236812004 68-69 0.1698438177926041 70-71 0.16398713442044532 72-73 0.16195522386275757 74-75 0.16422618272134976 76-77 0.1668557140312986 78-79 0.17008286609350853 80-81 0.16422618272134976 82-83 0.16225403423888812 84-85 0.15747306822079934 86-87 0.15436544030904165 88-89 0.15771211652170378 90-91 0.16368832404431477 92-93 0.15633758879150325 94-95 0.16159665141140092 96-97 0.16105879273436594 98-99 0.16046117198210483 100-101 0.14450469789673354 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 836651.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.56272266404764 #Duplication Level Percentage of deduplicated Percentage of total 1 85.16470666116142 66.90771230185796 2 10.744114695386322 16.881738061687088 3 2.4924615031770543 5.874436854747427 4 0.8203785133959717 2.5780467850988567 5 0.29130110157320965 1.1442703827313823 6 0.15331412147215456 0.7226864883419396 7 0.0908697356729479 0.4997281689560387 8 0.04906294099871277 0.30836145814115234 9 0.03560689815381446 0.2517637378126604 >10 0.1407794718667752 1.945801007656135 >50 0.009466350819185456 0.5260894829797438 >100 0.007022082610952365 0.9912140869657912 >500 3.0530790387455457E-4 0.1729377634923085 >1k 6.106158077491091E-4 1.195213419531447 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3981 0.47582564295028634 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3029 0.36203865171977323 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1889 0.22578112020424285 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1067 0.12753226853251834 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.1952415045221963E-4 0.0 6 0.0 0.0 0.0 1.1952415045221963E-4 0.0 7 0.0 0.0 0.0 1.1952415045221963E-4 0.0 8 0.0 0.0 0.0 1.1952415045221963E-4 0.0 9 0.0 0.0 0.0 2.3904830090443926E-4 0.0 10-11 0.0 0.0 0.0 2.9881037613054906E-4 0.0 12-13 0.0 0.0 0.0 4.183345265827687E-4 0.0 14-15 0.0 0.0 0.0 5.378586770349883E-4 0.0 16-17 0.0 0.0 0.0 5.976207522610981E-4 0.0 18-19 0.0 0.0 0.0 5.976207522610981E-4 0.0 20-21 0.0 0.0 0.0 5.976207522610981E-4 0.0 22-23 0.0 0.0 0.0 5.976207522610981E-4 0.0 24-25 0.0 0.0 0.0 7.171449027133177E-4 0.0 26-27 0.0 0.0 0.0 8.366690531655374E-4 0.0 28-29 0.0 0.0 0.0 0.0020916726329138434 0.0 30-31 0.0 0.0 0.0 0.006812876575776518 0.0 32-33 0.0 0.0 0.0 0.014103849753361916 0.0 34-35 0.0 0.0 0.0 0.019482436523711802 0.0 36-37 0.0 0.0 0.0 0.023307209338182824 0.0 38-39 0.0 0.0 0.0 0.027908889130593282 0.0 40-41 0.0 0.0 0.0 0.033885096653204265 0.0 42-43 0.0 0.0 0.0 0.03992106625104136 0.0 44-45 0.0 0.0 0.0 0.047988946406566174 0.0 46-47 0.0 0.0 0.0 0.05856683372158762 0.0 48-49 0.0 0.0 0.0 0.07440378365650671 0.0 50-51 0.0 0.0 0.0 0.09042001981710415 0.0 52-53 0.0 0.0 0.0 0.12878727211226665 0.0 54-55 0.0 0.0 0.0 0.18424647792209653 0.0 56-57 0.0 0.0 0.0 0.2545266783880017 0.0 58-59 0.0 0.0 0.0 0.34638098801053246 0.0 60-61 0.0 0.0 0.0 0.4684749076974748 0.0 62-63 0.0 0.0 0.0 0.5561458720541779 0.0 64-65 0.0 0.0 0.0 0.6521835269425363 0.0 66-67 0.0 0.0 0.0 0.814138750805294 0.0 68-69 0.0 0.0 0.0 1.016015040919093 0.0 70-71 0.0 0.0 0.0 1.1931498318892824 0.0 72-73 0.0 0.0 0.0 1.3703443849346981 0.0 74-75 0.0 0.0 0.0 1.6799119346059468 0.0 76-77 0.0 0.0 0.0 1.9451957865346483 0.0 78-79 0.0 0.0 0.0 2.1448608798650812 0.0 80-81 0.0 0.0 0.0 2.316378035764016 0.0 82-83 0.0 0.0 0.0 2.5974390755524106 0.0 84-85 0.0 0.0 0.0 2.930791931163651 0.0 86-87 0.0 0.0 0.0 3.3683698459692275 0.0 88-89 0.0 0.0 0.0 4.029936018722263 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2990 0.0 33.40102 1 GTATCAA 4420 0.0 28.83528 1 CGATATA 35 0.008374485 27.15491 46-47 TCAACGC 4945 0.0 25.47928 4 ATCAACG 4930 0.0 25.458838 3 GTATAGC 600 0.0 25.36357 1 CAACGCA 5010 0.0 25.148708 5 TATCAAC 5065 0.0 24.967995 2 GTACATG 3205 0.0 24.928312 1 AACGCAG 5155 0.0 24.511528 6 TACATGG 3195 0.0 24.106028 2 ACATGGG 3225 0.0 23.144693 3 ACGCAGA 5680 0.0 22.245937 7 CGCAGAG 5640 0.0 22.149712 8 GAGTACT 3390 0.0 22.069794 12-13 ATAGCCC 630 0.0 21.884619 3 TAGCCCT 965 0.0 21.678684 4 CATGGGG 1530 0.0 20.820488 4 GCAGAGT 6045 0.0 20.665735 9 GTACTTT 3625 0.0 20.506792 14-15 >>END_MODULE