FastQCFastQC Report
Thu 26 May 2016
SRR937711_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937711_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836651
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48610.5810068953482396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39500.4721203942862675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26040.3112408877775799No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10390.1241855923198562No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9860.11785081234588854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTTA500.001615030538.002568
GGTATCA35750.034.6868741
GTATCAA51650.029.4361081
ACATCGT650.00584466529.2292466
CGTGTAC700.00838508827.1463053
GTGTAGC6300.024.887091
ATCAACG60550.024.7925823
TATCAAC61050.024.6673452
TCAACGC60950.024.6298734
CAACGCA61250.024.5092375
AACGCAG63400.023.6738436
GTATAGC5700.023.3390881
ACACCGT1452.3343036E-522.9298366
GAGTACT44350.022.76083412-13
TAGCCCT9200.022.7202784
GTAGCCC5450.022.663433
AGCGAGT1050.00219486722.620578
GTACATG32850.022.4180091
GTACTTT46700.021.61289814-15
CGCAGAG70650.021.179778
Produced by FastQC (version 0.11.5)