##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937711_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 836651 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30385070955512 33.0 31.0 34.0 31.0 34.0 2 32.59745341845046 34.0 31.0 34.0 31.0 34.0 3 32.69261496131601 34.0 31.0 34.0 31.0 34.0 4 36.28146144569241 37.0 35.0 37.0 35.0 37.0 5 36.21832759418204 37.0 35.0 37.0 35.0 37.0 6 36.26108616376482 37.0 37.0 37.0 35.0 37.0 7 36.214484892745006 37.0 36.0 37.0 35.0 37.0 8 36.19587498251959 37.0 36.0 37.0 35.0 37.0 9 37.92815164268016 39.0 38.0 39.0 35.0 39.0 10-11 37.8512223137246 39.0 38.0 39.0 35.0 39.0 12-13 37.76957715941295 39.0 37.0 39.0 35.0 39.0 14-15 39.04809054193445 40.0 38.0 41.0 36.0 41.0 16-17 39.01479111361846 40.0 38.0 41.0 36.0 41.0 18-19 39.010465534613594 40.0 38.0 41.0 36.0 41.0 20-21 38.9380595971319 40.0 38.0 41.0 35.5 41.0 22-23 38.88644129989685 40.0 38.0 41.0 35.0 41.0 24-25 38.91756120532934 40.0 38.0 41.0 35.0 41.0 26-27 38.80242359119872 40.0 38.0 41.0 35.0 41.0 28-29 38.66341999232655 40.0 38.0 41.0 35.0 41.0 30-31 38.68880273853733 40.0 38.0 41.0 35.0 41.0 32-33 38.587580126002365 40.0 38.0 41.0 35.0 41.0 34-35 38.45062756155195 40.0 38.0 41.0 35.0 41.0 36-37 38.317815911293955 40.0 38.0 41.0 34.0 41.0 38-39 38.19400861291028 40.0 38.0 41.0 34.0 41.0 40-41 38.00592780024168 40.0 38.0 41.0 34.0 41.0 42-43 37.769261018034996 40.0 38.0 41.0 33.0 41.0 44-45 37.43476132820017 40.0 37.0 41.0 33.0 41.0 46-47 37.00969759194694 40.0 37.0 41.0 32.0 41.0 48-49 36.63622884571942 40.0 37.0 41.0 31.0 41.0 50-51 36.36900272634587 40.0 37.0 41.0 30.0 41.0 52-53 36.14943447148214 40.0 36.0 41.0 30.0 41.0 54-55 35.939964214469356 39.0 36.0 41.0 29.0 41.0 56-57 35.680287838059115 39.0 35.5 40.5 28.0 41.0 58-59 35.46701790830346 39.0 35.0 40.0 28.0 41.0 60-61 35.18466062910342 39.0 35.0 40.0 27.0 41.0 62-63 34.85243369098943 38.0 34.5 40.0 26.0 41.0 64-65 34.503892303959475 38.0 34.0 40.0 26.0 41.0 66-67 34.12933588796284 37.5 34.0 40.0 25.5 41.0 68-69 33.717939738313824 37.0 34.0 39.5 24.0 41.0 70-71 33.34807942618846 36.5 33.0 39.0 23.0 40.0 72-73 32.870962324792536 36.0 32.5 39.0 22.0 40.0 74-75 32.406053420123804 35.5 32.0 38.0 20.5 40.0 76-77 31.138664150284885 34.0 30.5 36.5 20.0 39.0 78-79 31.68576204414983 35.0 32.0 37.0 20.0 39.0 80-81 31.471741502729333 35.0 32.0 36.5 19.0 39.0 82-83 31.078251266059564 35.0 31.0 36.0 18.0 37.5 84-85 30.667485606304183 34.0 31.0 36.0 14.5 37.0 86-87 30.285930453677818 34.0 31.0 35.0 7.5 37.0 88-89 29.86164960061005 34.0 30.5 35.0 4.5 36.0 90-91 29.349972688731622 34.0 30.0 35.0 2.0 36.0 92-93 28.945645794961102 34.0 29.0 35.0 2.0 35.5 94-95 28.51494649501405 34.0 29.0 35.0 2.0 35.0 96-97 28.011143834167413 34.0 29.0 35.0 2.0 35.0 98-99 27.22518588993499 33.0 27.0 35.0 2.0 35.0 100-101 24.53455502951649 31.0 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17001474304397846 1101 2 0.15826104113942563 1101 3 0.09343361465259648 1101 4 0.0360902774587899 1101 5 -0.015516344370794855 1101 6 0.07085077329048772 1101 7 0.12260238405973922 1101 8 0.0957064441130413 1101 9 0.1201434228500986 1101 10-11 0.1504037250353818 1101 12-13 0.1407930304932492 1101 14-15 0.1815359870867823 1101 16-17 0.1597117047116967 1101 18-19 0.28184887023641636 1101 20-21 0.20356870014790474 1101 22-23 0.07407065175761574 1101 24-25 0.11825716778070472 1101 26-27 -0.012812963295381508 1101 28-29 -0.09558762048694547 1101 30-31 0.05401466514737763 1101 32-33 0.10567957531802818 1101 34-35 -0.08108850825617253 1101 36-37 -0.05136413144942509 1101 38-39 -0.1299836146203006 1101 40-41 -0.03614682490068333 1101 42-43 -0.1491863363064354 1101 44-45 -0.1057655606750032 1101 46-47 -0.10842928784837369 1101 48-49 -0.19244034144499977 1101 50-51 -0.3419258633813058 1101 52-53 -0.25410184600369945 1101 54-55 -0.2751708528463723 1101 56-57 -0.4271623932805184 1101 58-59 -0.3878269608121272 1101 60-61 -0.30966544468697776 1101 62-63 -0.42218270401956204 1101 64-65 -0.4006514203080158 1101 66-67 -0.2985949161247632 1101 68-69 -0.1927347637913499 1101 70-71 -0.3599208076641034 1101 72-73 -0.3618675618235727 1101 74-75 -0.3921864291763413 1101 76-77 -0.442844912712264 1101 78-79 -0.43133278105071327 1101 80-81 -0.6299267581403001 1101 82-83 -0.5372268007171321 1101 84-85 -0.4823372683525946 1101 86-87 -0.7497469761304068 1101 88-89 -0.5541896342147332 1101 90-91 -0.49125320632861147 1101 92-93 -0.7135849654388657 1101 94-95 -0.8114044362704433 1101 96-97 -0.9468372829034806 1101 98-99 -0.7413289657935813 1101 100-101 -0.5598235857733478 1102 1 0.08163186155765345 1102 2 0.1246969041270134 1102 3 0.019760161668799014 1102 4 0.008508663670284022 1102 5 -0.03918233355403089 1102 6 -0.04556155001838391 1102 7 -0.006440986362939327 1102 8 -0.0100154202295073 1102 9 -0.03858290829002442 1102 10-11 0.07590925398918813 1102 12-13 0.14551819039082403 1102 14-15 0.1324356621243865 1102 16-17 0.08159441969785775 1102 18-19 0.09074112740454154 1102 20-21 0.02604507434840997 1102 22-23 -0.036278768936632844 1102 24-25 -0.07311467876355948 1102 26-27 -0.16283535469708 1102 28-29 -0.08284748705715828 1102 30-31 -0.009720450311874629 1102 32-33 -0.030839078441999845 1102 34-35 -0.010038868241025511 1102 36-37 0.015385211161991208 1102 38-39 0.0988852176968038 1102 40-41 0.15896266331225206 1102 42-43 0.1387900551182284 1102 44-45 -0.04159409995181562 1102 46-47 -0.006611565340996606 1102 48-49 -0.00796389834786737 1102 50-51 0.3271234780818304 1102 52-53 0.2985925860010781 1102 54-55 0.19669061901510076 1102 56-57 0.26961209659741314 1102 58-59 0.22127657932588818 1102 60-61 0.11384208226883175 1102 62-63 -0.0018731285375679363 1102 64-65 0.21730533557977338 1102 66-67 0.14219992122179548 1102 68-69 0.006537228584093668 1102 70-71 -0.02891847666176517 1102 72-73 -0.09240713719073312 1102 74-75 -0.16402952860564568 1102 76-77 -0.25857515947699383 1102 78-79 -0.2387667264156974 1102 80-81 -0.08672857701108327 1102 82-83 -0.03290927915823616 1102 84-85 0.09437911870861626 1102 86-87 -0.0938568892058349 1102 88-89 -0.026389847794256127 1102 90-91 -0.10760811578697016 1102 92-93 -0.22278840567679126 1102 94-95 -0.5907515259623608 1102 96-97 -0.3433028862656329 1102 98-99 -0.2574220680935788 1102 100-101 -0.538620650777311 1103 1 0.26712601145669623 1103 2 0.27502377414242574 1103 3 0.1796236574611001 1103 4 0.11277480909326698 1103 5 0.12180795535617506 1103 6 0.15444533964367935 1103 7 0.04251339629555417 1103 8 0.1378745096403975 1103 9 0.20299642450176236 1103 10-11 0.23337470288956297 1103 12-13 0.31034168724992384 1103 14-15 0.2995121896013657 1103 16-17 0.28316537377159534 1103 18-19 0.23814711407637645 1103 20-21 0.24196152719478903 1103 22-23 0.2069624170186799 1103 24-25 0.12499866506725255 1103 26-27 0.1694602695896421 1103 28-29 0.10679212805521132 1103 30-31 0.21983864465322966 1103 32-33 0.1430443240287218 1103 34-35 0.3502493418803425 1103 36-37 0.4329787411025663 1103 38-39 0.2858656455003725 1103 40-41 0.03482428778344371 1103 42-43 -0.034344930030108856 1103 44-45 -0.07129965579752451 1103 46-47 -0.1636252968819818 1103 48-49 -0.3321747264519104 1103 50-51 -0.345380880740656 1103 52-53 -0.41173490630639265 1103 54-55 -0.5614316813763907 1103 56-57 -0.4574822773267613 1103 58-59 -0.4610227676008165 1103 60-61 -0.4330854062604388 1103 62-63 -0.405177277114241 1103 64-65 -0.4018985973060367 1103 66-67 -0.4856377583400189 1103 68-69 -0.31502552456932165 1103 70-71 -0.2947305289031874 1103 72-73 -0.45746199334681137 1103 74-75 -0.31764232293034667 1103 76-77 -0.4145137703655486 1103 78-79 -0.29876123691076373 1103 80-81 -0.26790835385270384 1103 82-83 -0.3011732732286809 1103 84-85 -0.25617688813215267 1103 86-87 -0.22630341931643727 1103 88-89 -0.3730287483819872 1103 90-91 -0.14988063748107194 1103 92-93 -0.09887838910826474 1103 94-95 -0.21515270876176373 1103 96-97 -0.046291221623267376 1103 98-99 -0.08061167048229123 1103 100-101 -0.04955965615458169 1104 1 0.22748600741179814 1104 2 0.11787039730050708 1104 3 0.10267724458587679 1104 4 0.12189527705689329 1104 5 0.08186328749159344 1104 6 0.05883405437722189 1104 7 0.1390801591582047 1104 8 0.07107016085607398 1104 9 0.10061123090411428 1104 10-11 0.20070112878105562 1104 12-13 0.10289414776678285 1104 14-15 -0.017081487392758277 1104 16-17 0.035224123327566303 1104 18-19 0.07983536649878431 1104 20-21 0.06642136472470384 1104 22-23 0.22329499063712177 1104 24-25 0.15965255400367795 1104 26-27 0.14643870457697972 1104 28-29 0.1962900420803706 1104 30-31 0.14046269987127147 1104 32-33 0.2160806684777441 1104 34-35 0.3053956971935463 1104 36-37 0.30168224745901995 1104 38-39 0.1907100595216491 1104 40-41 0.1499716543212486 1104 42-43 0.27232318865136307 1104 44-45 0.09859904024132504 1104 46-47 0.05524324651381107 1104 48-49 0.22089058050661237 1104 50-51 0.14730329826164734 1104 52-53 0.046095083503573164 1104 54-55 -0.08111490879042549 1104 56-57 0.15172997871530214 1104 58-59 0.2210268290761448 1104 60-61 0.20974617817292796 1104 62-63 0.30856643190199406 1104 64-65 0.08152444979888429 1104 66-67 -0.18047740145551927 1104 68-69 0.09769606974293055 1104 70-71 -0.10001404775734102 1104 72-73 0.2843827395991454 1104 74-75 0.23340636883025212 1104 76-77 -0.0901602410620761 1104 78-79 0.1913864537374863 1104 80-81 0.0416430513605448 1104 82-83 0.18453993829098536 1104 84-85 0.33463885896835777 1104 86-87 -0.11350390885285577 1104 88-89 0.018814947410536575 1104 90-91 0.05980780162894561 1104 92-93 0.16998548709710448 1104 94-95 0.010896825685989597 1104 96-97 -0.28169782466057214 1104 98-99 -0.09957991025142121 1104 100-101 -0.1960465582032178 1105 1 0.1730108434005473 1105 2 0.1699815164219345 1105 3 0.17825759207344305 1105 4 0.06313058813756811 1105 5 0.07049664725897031 1105 6 0.0769924423809698 1105 7 0.11926964321366995 1105 8 0.08380956921403993 1105 9 0.085033166356979 1105 10-11 0.20553735784409355 1105 12-13 0.10806080241797389 1105 14-15 0.09795017338632306 1105 16-17 0.18045818479874498 1105 18-19 0.20915943590016894 1105 20-21 0.008919802712831881 1105 22-23 0.010493402191208645 1105 24-25 0.12589958017337466 1105 26-27 0.16526244956051528 1105 28-29 0.18651845627785946 1105 30-31 -0.002215069095150568 1105 32-33 0.1829870438892769 1105 34-35 0.2388404740661585 1105 36-37 0.2532658871869913 1105 38-39 0.2930428641740406 1105 40-41 0.052078568026097116 1105 42-43 0.26895885539011744 1105 44-45 0.36339009111485154 1105 46-47 0.3478020622685065 1105 48-49 0.4752867427636005 1105 50-51 0.6123112284757681 1105 52-53 0.42647951865605194 1105 54-55 0.2991809122270368 1105 56-57 0.25206971629730646 1105 58-59 0.29940978632507864 1105 60-61 0.2629822462285816 1105 62-63 0.04608085047311761 1105 64-65 0.4155381594569221 1105 66-67 0.40480394091343896 1105 68-69 0.28786801120353545 1105 70-71 0.2151924381965742 1105 72-73 0.3234765924816543 1105 74-75 0.26276918814151884 1105 76-77 0.11820012709210914 1105 78-79 0.10090391222401607 1105 80-81 0.15385107529537478 1105 82-83 0.13597338456979102 1105 84-85 0.4870701905234007 1105 86-87 0.24128155482435787 1105 88-89 0.1380610363731236 1105 90-91 -0.0020223355877888594 1105 92-93 0.0300592621193303 1105 94-95 0.03336726761519415 1105 96-97 -0.22468889716195406 1105 98-99 0.01497563729381568 1105 100-101 0.4026861094727501 1106 1 0.21639572312563615 1106 2 0.09781656796832294 1106 3 0.10995862300127612 1106 4 0.14775258126471158 1106 5 0.0756512864342298 1106 6 -0.09611752325476886 1106 7 0.08361672225147032 1106 8 0.043002009076580805 1106 9 0.08417405982776671 1106 10-11 0.08526244375475045 1106 12-13 -0.2570594724961168 1106 14-15 -0.10973883005010521 1106 16-17 0.09497708514238212 1106 18-19 0.13806837060807453 1106 20-21 0.18396275803929285 1106 22-23 0.1812408248716153 1106 24-25 0.005409889451733818 1106 26-27 -0.09998669133295834 1106 28-29 0.0829961195081026 1106 30-31 -0.017678986620929038 1106 32-33 -0.2951057396158774 1106 34-35 -0.5053605568616746 1106 36-37 -0.08608119185080909 1106 38-39 0.055499908847579604 1106 40-41 -0.3272169646199501 1106 42-43 -0.4286356463280896 1106 44-45 -0.1391872284727782 1106 46-47 -0.3042597934015987 1106 48-49 -0.4536221919443051 1106 50-51 -0.24035200176478355 1106 52-53 -0.4629105157082094 1106 54-55 -0.3898225241990758 1106 56-57 -0.19402134899478796 1106 58-59 -0.2547139250151318 1106 60-61 -0.48852119019753104 1106 62-63 -0.4386700086334159 1106 64-65 -0.7182676824674772 1106 66-67 -0.67917372231679 1106 68-69 -0.27183989257653707 1106 70-71 -0.34601890200961805 1106 72-73 -0.4769959161094022 1106 74-75 -0.44212771907496773 1106 76-77 -0.4522466083030814 1106 78-79 -0.35528165478629603 1106 80-81 -0.266896347385039 1106 82-83 -0.5221022168047789 1106 84-85 -0.7728095345625086 1106 86-87 -0.25034215651584546 1106 88-89 -0.43775511482962415 1106 90-91 -0.8755188991616727 1106 92-93 -0.7766417688085063 1106 94-95 -0.7464866842748421 1106 96-97 -0.37138133702449494 1106 98-99 -0.4575329537714765 1106 100-101 -0.896068186059896 1107 1 0.1858599458776169 1107 2 0.11878245104563945 1107 3 0.10593279454893434 1107 4 0.13414107107910667 1107 5 0.1836224267698725 1107 6 0.08521578902848148 1107 7 -0.004767315470282085 1107 8 0.053787545564269124 1107 9 -0.013051012926581507 1107 10-11 0.08758058419038406 1107 12-13 0.15499722590354992 1107 14-15 0.12137398260941978 1107 16-17 0.07657799661099318 1107 18-19 0.18609676717059642 1107 20-21 0.20493127237703135 1107 22-23 0.15501768346242528 1107 24-25 0.04367894109284265 1107 26-27 0.24524125475777936 1107 28-29 0.09719834765943602 1107 30-31 0.15379367721450166 1107 32-33 0.20122365208933246 1107 34-35 0.09005552434954467 1107 36-37 0.10386028891731058 1107 38-39 0.10307704424698727 1107 40-41 0.012280096262145435 1107 42-43 0.08698351556284223 1107 44-45 0.04682972736940627 1107 46-47 -0.08133546318894247 1107 48-49 -0.03404271998564923 1107 50-51 0.05789347378291865 1107 52-53 0.07893705011276353 1107 54-55 -0.02901093823317069 1107 56-57 -0.004611382832798938 1107 58-59 0.10971432840682382 1107 60-61 -0.025279898353915087 1107 62-63 -0.12822074722821952 1107 64-65 -0.08541797480050661 1107 66-67 0.0594855452455505 1107 68-69 0.15770153135635212 1107 70-71 0.30389189826193075 1107 72-73 0.44072588598822904 1107 74-75 0.18128365744260577 1107 76-77 0.3087204851540264 1107 78-79 0.3333678771124795 1107 80-81 0.110454025990812 1107 82-83 0.25924267302434245 1107 84-85 0.4023521563906911 1107 86-87 0.2249858300962977 1107 88-89 0.33633012748314783 1107 90-91 0.4159326619651402 1107 92-93 0.5712766410681986 1107 94-95 -0.05481120288574459 1107 96-97 -0.01929764189285521 1107 98-99 0.12819614218006592 1107 100-101 0.12407805939460204 1108 1 0.3253435638680813 1108 2 0.16103477008271483 1108 3 0.13696302250222914 1108 4 0.05800057303480344 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-0.6008584783984432 2316 54-55 -0.6279177622943166 2316 56-57 -0.5819693116850146 2316 58-59 -0.6055975774451738 2316 60-61 -0.45194779795404116 2316 62-63 -0.6649832143280605 2316 64-65 -0.8321606623398381 2316 66-67 -0.624559092959899 2316 68-69 -0.5834501036712822 2316 70-71 -0.36590108168561386 2316 72-73 -0.45036361370390665 2316 74-75 -0.47600106222666483 2316 76-77 -0.5257615813025893 2316 78-79 -0.4476969714284138 2316 80-81 -0.6700627685926968 2316 82-83 -0.37801777890591026 2316 84-85 -0.5635557299478791 2316 86-87 -0.48708737006518277 2316 88-89 -0.48095590029845425 2316 90-91 -0.3576003915524417 2316 92-93 -0.3230335213858204 2316 94-95 0.02388641341784492 2316 96-97 0.18188326778649966 2316 98-99 0.05694912772091243 2316 100-101 0.00439818605595832 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 3.0 5 17.0 6 23.0 7 57.0 8 152.0 9 291.0 10 544.0 11 958.0 12 1668.0 13 2576.0 14 3293.0 15 4853.0 16 7133.0 17 10739.0 18 9963.0 19 5460.0 20 3653.0 21 3332.0 22 3359.0 23 3638.0 24 4233.0 25 4803.0 26 5840.0 27 6873.0 28 8799.0 29 11138.0 30 14301.0 31 18384.0 32 24500.0 33 33294.0 34 47502.0 35 69615.0 36 113705.0 37 195838.0 38 195865.0 39 20242.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.62127979036762 21.20471672851077 15.42452469301098 21.749478788110633 2 14.89665344331149 21.816265085441838 41.26774485418651 22.01933661706016 3 18.656046547485154 25.757334898302876 31.23333385127132 24.353284702940652 4 12.152498473078978 18.539271452493335 39.17965794578623 30.12857212864145 5 12.469092404742433 38.82197225131641 35.457767192275135 13.25116815166602 6 29.639479304990967 38.549287576301225 18.41795443978433 13.39327867892347 7 26.43037538949933 34.31036358051326 21.961486928241285 17.29777410174613 8 24.44794783009881 36.640725941880184 20.801624572252948 18.109701655768056 9 25.428763008709726 17.153388927999845 22.00630848466087 35.41153957862956 10-11 23.43127540635223 28.15445149769737 28.63338476855941 19.78088832739099 12-13 24.7997313016808 25.695531814693783 29.006270424299508 20.49846645932591 14-15 21.44203497037594 27.53842402626663 28.06271671222529 22.956824291132143 16-17 20.058303880590593 30.390927638884076 29.01221656341772 20.53855191710761 18-19 19.708755502593075 28.823129357402312 31.99034005816045 19.47777508184416 20-21 21.098223751600127 27.740300316380427 31.998109127939845 19.163366804079597 22-23 20.31582055014905 28.190955314434063 31.59711514786421 19.89610898755268 24-25 20.103998149762834 27.958302730115776 32.19585979552967 19.74183932459172 26-27 20.199332093459105 28.241641606226782 31.974949918961947 19.584076381352165 28-29 19.884215917532376 28.640036048806927 31.86793419180332 19.607813841857375 30-31 20.34211397583939 28.118116155959893 32.047412840001385 19.492357028199333 32-33 20.011569937763777 28.206085930692726 31.889581199329232 19.892762932214268 34-35 20.428649460766795 28.46885977546193 31.74328363917571 19.35920712459556 36-37 20.2041233441423 28.37622855886146 31.647664318813938 19.7719837781823 38-39 20.133305284999363 28.548522621738336 31.762108692871937 19.556063400390368 40-41 20.70274938220327 28.299960855443036 31.47353857830555 19.523751184048145 42-43 20.22113362811442 28.51600556091485 31.257553317004422 20.005307493966313 44-45 20.648275087222746 28.621372591438966 30.883546424972895 19.846805896365392 46-47 20.73648391025649 28.721892401969278 30.391286211335434 20.1503374764388 48-49 20.700148568519012 28.823009833251856 30.295905939274558 20.18093565895457 50-51 20.925033257594862 29.32148530271284 29.666192952617042 20.087288487075256 52-53 21.005293724623527 29.403299583697383 29.35238229560474 20.23902439607435 54-55 21.020592815881415 29.205487114698958 29.33762106302389 20.436299006395735 56-57 21.150814377799108 29.353338488808355 29.06588290697077 20.42996422642177 58-59 21.155236771365836 29.3571034995476 29.037376397087915 20.450283331998648 60-61 21.172806821482315 29.14387241514084 29.461029748365807 20.222291015011038 62-63 21.117347615672486 29.10287563153573 29.439216590908273 20.34056016188351 64-65 21.03433809318342 29.237459824944928 29.295189989613352 20.433012092258302 66-67 20.907044872951804 29.13592405913577 29.184988722896403 20.772042345016022 68-69 21.138622914452977 29.076401032210562 29.2534162990303 20.531559754306155 70-71 21.05394005385758 29.037495921238367 29.20847521846027 20.700088806443787 72-73 21.053043622729188 28.979646232419494 29.251145340171707 20.71616480467961 74-75 20.98174746698444 29.253595585255983 28.896158613328616 20.868498334430964 76-77 20.765588040891604 28.9691281071797 29.292321410002497 20.972962441926203 78-79 20.689929253655347 29.057277168138206 29.03068304466259 21.222110533543855 80-81 20.67283730014068 29.182956812338716 29.01394966359928 21.13025622392132 82-83 20.694605138600703 29.325534752966597 29.075011235310427 20.90484887312227 84-85 20.857574339583852 29.133849797496087 29.02585966302536 20.9827161998947 86-87 20.40815106896424 29.320349823283543 28.83496224829708 21.43653685945514 88-89 20.759073974691955 29.070066252236597 28.75840702993243 21.412452743139017 90-91 20.719855404922402 28.98589791558832 28.93772346430488 21.356523215184396 92-93 20.491443720514393 28.88026706923074 29.229701770301812 21.39858743995305 94-95 20.344564220923658 29.093134413273873 28.95675735760789 21.605544008194578 96-97 20.288148821910212 29.070544348838407 29.1657453346736 21.475561494577786 98-99 20.382948231647884 28.94684156456686 28.952698321441527 21.717511882343732 100-101 20.056903595625837 29.485566391014974 28.67833628710016 21.779193726259034 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 61.0 1 45.5 2 35.5 3 41.5 4 40.0 5 41.5 6 77.5 7 158.0 8 268.0 9 356.0 10 391.5 11 445.0 12 510.0 13 596.5 14 709.5 15 795.5 16 944.0 17 1116.5 18 1303.0 19 1586.5 20 1871.0 21 2182.5 22 2679.5 23 3386.0 24 4355.5 25 5541.5 26 6941.5 27 8718.0 28 10656.5 29 12884.0 30 15217.0 31 17689.0 32 20730.0 33 23594.5 34 26315.0 35 28985.5 36 31184.0 37 32735.5 38 33433.5 39 33557.5 40 33381.0 41 33108.5 42 32845.0 43 32613.0 44 32149.5 45 31523.5 46 31137.0 47 31198.0 48 32048.5 49 30657.0 50 27620.0 51 24882.0 52 22054.5 53 19432.5 54 16753.5 55 14594.5 56 12490.5 57 10520.5 58 8603.5 59 6739.5 60 5251.0 61 3985.5 62 2901.0 63 2085.0 64 1483.5 65 1075.5 66 865.5 67 688.5 68 476.5 69 308.0 70 222.5 71 185.0 72 152.0 73 126.0 74 89.0 75 77.0 76 59.5 77 33.5 78 21.5 79 15.5 80 14.5 81 11.5 82 8.5 83 5.5 84 1.5 85 0.5 86 1.5 87 2.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016613856912858528 2 0.0 3 0.0 4 0.0 5 0.034303431179787035 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0029881037613054906 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.976207522610981E-4 24-25 1.7928622567832942E-4 26-27 8.366690531655375E-4 28-29 8.964311283916472E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0010159552788438669 42-43 0.011713366744317524 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.5857245135665884E-4 84-85 5.9762075226109814E-5 86-87 0.0 88-89 0.0 90-91 0.01290860824883972 92-93 0.001673338106331075 94-95 0.0 96-97 0.0 98-99 0.001255003579748306 100-101 0.017749336342154615 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 836651.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.275664036243 #Duplication Level Percentage of deduplicated Percentage of total 1 83.95971040853841 64.04082663701931 2 11.209730844163738 17.10059327612288 3 2.871094481614244 6.56983914287758 4 0.942978435978524 2.8770522550447915 5 0.40450430082249295 1.5426917075375923 6 0.20773099417144658 0.9506891712801607 7 0.11240098063580532 0.6001421605424659 8 0.06427412267753024 0.39220411100604524 9 0.044410980378545434 0.30487293169867 >10 0.1624877733357052 2.155403911828357 >50 0.011680872692713836 0.626783947862838 >100 0.008048878195955558 1.1552784199596993 >500 3.1564226492507286E-4 0.18442080689978768 >1k 6.312845298501457E-4 1.4992015203198146 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4861 0.5810068953482396 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3950 0.4721203942862675 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2604 0.3112408877775799 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1039 0.1241855923198562 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 986 0.11785081234588854 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.1952415045221963E-4 0.0 3 0.0 0.0 0.0 1.1952415045221963E-4 0.0 4 0.0 0.0 0.0 1.1952415045221963E-4 0.0 5 0.0 0.0 0.0 1.1952415045221963E-4 0.0 6 0.0 0.0 0.0 1.1952415045221963E-4 0.0 7 0.0 0.0 0.0 1.1952415045221963E-4 0.0 8 0.0 0.0 0.0 1.1952415045221963E-4 0.0 9 0.0 0.0 0.0 1.1952415045221963E-4 0.0 10-11 0.0 0.0 0.0 1.1952415045221963E-4 0.0 12-13 0.0 0.0 0.0 1.1952415045221963E-4 0.0 14-15 0.0 0.0 0.0 1.1952415045221963E-4 0.0 16-17 0.0 0.0 0.0 1.1952415045221963E-4 0.0 18-19 0.0 0.0 0.0 1.1952415045221963E-4 0.0 20-21 0.0 0.0 0.0 1.1952415045221963E-4 0.0 22-23 0.0 0.0 0.0 1.1952415045221963E-4 0.0 24-25 0.0 0.0 0.0 2.3904830090443926E-4 0.0 26-27 0.0 0.0 0.0 3.5857245135665884E-4 0.0 28-29 0.0 0.0 0.0 0.001553813955878855 0.0 30-31 0.0 0.0 0.0 0.006275017898741531 0.0 32-33 0.0 0.0 0.0 0.013565991076326927 0.0 34-35 0.0 0.0 0.0 0.018645767470546262 0.0 36-37 0.0 0.0 0.0 0.022590064435469508 0.0 38-39 0.0 0.0 0.0 0.027012458002201638 0.0 40-41 0.0 0.0 0.0 0.033227713825717056 0.0 42-43 0.0 0.0 0.0 0.03932344549878025 0.0 44-45 0.0 0.0 0.0 0.047690136030435634 0.0 46-47 0.0 0.0 0.0 0.05814849919500485 0.0 48-49 0.0 0.0 0.0 0.07404521120515006 0.0 50-51 0.0 0.0 0.0 0.0907785922684608 0.0 52-53 0.0 0.0 0.0 0.12932513078930163 0.0 54-55 0.0 0.0 0.0 0.1844257641477749 0.0 56-57 0.0 0.0 0.0 0.2551242991402628 0.0 58-59 0.0 0.0 0.0 0.34709813291324576 0.0 60-61 0.0 0.0 0.0 0.4698494354276753 0.0 62-63 0.0 0.0 0.0 0.5583570688375439 0.0 64-65 0.0 0.0 0.0 0.6551716307038419 0.0 66-67 0.0 0.0 0.0 0.8185611443720261 0.0 68-69 0.0 0.0 0.0 1.0218119622160255 0.0 70-71 0.0 0.0 0.0 1.199902946389833 0.0 72-73 0.0 0.0 0.0 1.3796075065947448 0.0 74-75 0.0 0.0 0.0 1.6920436358768471 0.0 76-77 0.0 0.0 0.0 1.9604948777925324 0.0 78-79 0.0 0.0 0.0 2.1615344988531655 0.0 80-81 0.0 0.0 0.0 2.3347847549336582 0.0 82-83 0.0 0.0 0.0 2.6202084262135585 0.0 84-85 0.0 0.0 0.0 2.956728671811783 0.0 86-87 0.0 0.0 0.0 3.4016573218701707 0.0 88-89 0.0 0.0 0.0 4.075414957969333 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGTTA 50 0.0016150305 38.00256 8 GGTATCA 3575 0.0 34.686874 1 GTATCAA 5165 0.0 29.436108 1 ACATCGT 65 0.005844665 29.229246 6 CGTGTAC 70 0.008385088 27.146305 3 GTGTAGC 630 0.0 24.88709 1 ATCAACG 6055 0.0 24.792582 3 TATCAAC 6105 0.0 24.667345 2 TCAACGC 6095 0.0 24.629873 4 CAACGCA 6125 0.0 24.509237 5 AACGCAG 6340 0.0 23.673843 6 GTATAGC 570 0.0 23.339088 1 ACACCGT 145 2.3343036E-5 22.929836 6 GAGTACT 4435 0.0 22.760834 12-13 TAGCCCT 920 0.0 22.720278 4 GTAGCCC 545 0.0 22.66343 3 AGCGAGT 105 0.002194867 22.62057 8 GTACATG 3285 0.0 22.418009 1 GTACTTT 4670 0.0 21.612898 14-15 CGCAGAG 7065 0.0 21.17977 8 >>END_MODULE