FastQCFastQC Report
Thu 26 May 2016
SRR937710_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937710_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833859
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39390.47238202142088775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31160.3736842799562036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19780.2372103677000548No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10850.13011792161504523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44300.031.8757341
GACTGCG650.005843411729.2305097
GGTATCA29800.027.4422451
TCAACGC51450.027.3502664
CAACGCA51650.027.1506865
GATCGGT350.00838561927.14750930-31
ATCAACG51850.027.136013
GTACATG29850.027.0777171
TATCAAC52400.026.9418962
AACGCAG52250.026.8179456
TACATGG30600.026.0970152
ACATGGG31450.024.7871283
CGCAGAG57900.024.1204078
ACGCAGA57900.024.1189587
GTATAGC6600.023.052261
CATTCCG1050.00219506222.6202079
GCAGAGT61350.022.4542879
AGTACTT33800.022.2742112-13
GAGTACT33700.022.26983312-13
AGAGTAC49900.021.3700510-11
Produced by FastQC (version 0.11.5)