##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937710_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 833859 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.949544227501292 33.0 31.0 34.0 30.0 34.0 2 32.09394034243199 33.0 31.0 34.0 30.0 34.0 3 32.14647200545895 34.0 31.0 34.0 30.0 34.0 4 35.715266010200764 37.0 35.0 37.0 35.0 37.0 5 35.70466589675233 37.0 35.0 37.0 35.0 37.0 6 35.79094187386597 37.0 35.0 37.0 35.0 37.0 7 35.741110907239715 37.0 35.0 37.0 35.0 37.0 8 35.73552603018016 37.0 35.0 37.0 35.0 37.0 9 37.402430147063235 39.0 37.0 39.0 35.0 39.0 10-11 37.301581562350464 39.0 37.0 39.0 34.5 39.0 12-13 37.20382102969447 39.0 37.0 39.0 34.0 39.0 14-15 38.40756950515615 40.0 38.0 41.0 34.0 41.0 16-17 38.35557810133368 40.0 38.0 41.0 34.0 41.0 18-19 38.33882167128975 40.0 38.0 41.0 34.0 41.0 20-21 38.15486730970104 40.0 38.0 41.0 34.0 41.0 22-23 38.05191105450682 40.0 38.0 41.0 33.5 41.0 24-25 37.9311826100096 40.0 38.0 41.0 33.0 41.0 26-27 38.03893583927258 40.0 38.0 41.0 34.0 41.0 28-29 37.990540367136404 40.0 38.0 41.0 33.5 41.0 30-31 37.80713525907858 40.0 37.5 41.0 33.0 41.0 32-33 37.57806295788616 40.0 37.0 41.0 33.0 41.0 34-35 37.344844272233075 40.0 37.0 41.0 32.5 41.0 36-37 37.37483735259798 40.0 37.0 41.0 33.0 41.0 38-39 37.34353949528638 40.0 37.0 41.0 33.0 41.0 40-41 37.16594472206932 40.0 37.0 41.0 31.5 41.0 42-43 36.93946698422635 40.0 37.0 41.0 31.0 41.0 44-45 36.67949077721774 40.0 36.0 41.0 30.5 41.0 46-47 36.30837587649711 39.5 36.0 41.0 30.0 41.0 48-49 35.97859770057048 39.0 36.0 41.0 29.0 41.0 50-51 35.10182536855751 38.5 35.0 40.0 27.0 40.5 52-53 35.208239642433554 38.5 35.0 40.0 27.5 40.5 54-55 35.53210494819868 39.0 35.5 40.0 27.5 41.0 56-57 35.30265248681132 39.0 35.0 40.0 26.5 41.0 58-59 35.12473751557518 39.0 35.0 40.0 26.0 41.0 60-61 34.87808550366429 38.5 35.0 40.0 26.0 41.0 62-63 34.56190315149204 38.0 34.0 40.0 24.5 41.0 64-65 34.25413049448408 38.0 34.0 40.0 23.0 41.0 66-67 33.892493814901556 37.5 34.0 40.0 22.5 41.0 68-69 33.46112472252503 37.0 33.0 39.5 21.5 41.0 70-71 33.044473945834966 36.5 33.0 39.0 20.0 41.0 72-73 32.59761542419042 36.0 32.0 39.0 18.5 40.0 74-75 32.123309216546204 35.5 32.0 38.5 16.5 40.0 76-77 31.651509427852908 35.0 31.0 37.5 12.0 39.5 78-79 31.116016017096413 35.0 31.0 37.0 7.5 39.0 80-81 30.575792190286368 34.5 30.5 36.5 6.0 39.0 82-83 30.050842528533003 34.0 30.0 36.0 2.0 37.5 84-85 29.553485061623128 34.0 29.0 35.5 2.0 37.0 86-87 29.0305927021235 34.0 29.0 35.0 2.0 37.0 88-89 28.545278638235 34.0 29.0 35.0 2.0 36.0 90-91 28.040951767624982 34.0 27.5 35.0 2.0 36.0 92-93 27.51376911444261 33.0 26.5 35.0 2.0 35.5 94-95 26.915179304894473 33.0 25.5 35.0 2.0 35.0 96-97 26.19384452287497 32.5 25.0 35.0 2.0 35.0 98-99 25.314442849450565 32.0 21.0 34.0 2.0 35.0 100-101 22.754319974959795 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1805313516960254 1101 2 0.28417414286909093 1101 3 0.24038744913408294 1101 4 0.29733113627116836 1101 5 0.19601335918663665 1101 6 0.1750383334475174 1101 7 0.18904422522903985 1101 8 0.2708624930427135 1101 9 0.2363749568742648 1101 10-11 0.20013860308503695 1101 12-13 0.27227801483551417 1101 14-15 0.5654641940336944 1101 16-17 0.5240966400654727 1101 18-19 0.5439904773717004 1101 20-21 0.5377019640882281 1101 22-23 0.5502946345303599 1101 24-25 0.6102661759938215 1101 26-27 0.687915178080658 1101 28-29 0.46483076763445297 1101 30-31 0.3161398238327493 1101 32-33 0.43379920343546274 1101 34-35 0.5686806179781954 1101 36-37 0.5304399876181591 1101 38-39 0.5936172839569664 1101 40-41 0.7240979710700088 1101 42-43 0.7036253381260735 1101 44-45 0.7930111757821621 1101 46-47 0.9072507384473525 1101 48-49 0.987129917965909 1101 50-51 0.9132042814518471 1101 52-53 0.8548014757243863 1101 54-55 0.9070944262255765 1101 56-57 0.9420615123458234 1101 58-59 0.944493539591214 1101 60-61 1.1833619364170502 1101 62-63 1.253023404819082 1101 64-65 1.1644773727043187 1101 66-67 1.2513930331747432 1101 68-69 1.5130356082085186 1101 70-71 1.3778083694009737 1101 72-73 1.4130573009352645 1101 74-75 1.3939415281654313 1101 76-77 1.3184130975982669 1101 78-79 1.488420185776711 1101 80-81 1.6706480723394677 1101 82-83 1.8248734737467487 1101 84-85 1.9666562453607632 1101 86-87 1.9576946529269215 1101 88-89 2.110528008589469 1101 90-91 2.175062889097088 1101 92-93 2.6039412535423416 1101 94-95 2.7421007685040344 1101 96-97 2.6628004485508114 1101 98-99 2.6424750619303587 1101 100-101 3.089149712957891 1102 1 0.03271618106272811 1102 2 0.05780412593924211 1102 3 -0.011647681151430334 1102 4 0.005424686369671861 1102 5 0.01965398677383945 1102 6 -0.012420060007620748 1102 7 0.06600712486307003 1102 8 0.015427875721549356 1102 9 -0.05892084242758955 1102 10-11 -0.0057736658191203105 1102 12-13 0.16771590999208996 1102 14-15 0.2390450095726635 1102 16-17 0.039205095867067996 1102 18-19 0.1517357996978177 1102 20-21 0.10624589553078323 1102 22-23 0.23748775986194914 1102 24-25 0.1727123198063154 1102 26-27 0.22431858162274665 1102 28-29 0.18925942740527546 1102 30-31 0.15549716533927693 1102 32-33 0.10454232589471246 1102 34-35 0.31634135863382795 1102 36-37 0.3750781923105322 1102 38-39 0.36668378346529806 1102 40-41 0.5510383989458063 1102 42-43 0.5050983365344734 1102 44-45 0.3456888631847974 1102 46-47 0.5460898778297008 1102 48-49 0.5653147405741876 1102 50-51 0.2481137354726073 1102 52-53 0.23850843723853643 1102 54-55 0.3987308771070346 1102 56-57 0.4316526953064894 1102 58-59 0.31218388214190895 1102 60-61 0.39077373054194453 1102 62-63 0.4474860594602532 1102 64-65 0.47307966313405103 1102 66-67 0.33868652109574526 1102 68-69 0.2964560125650024 1102 70-71 0.43027063205359184 1102 72-73 0.3810815096083502 1102 74-75 0.3509207831844101 1102 76-77 0.40860763881958206 1102 78-79 0.5264692654483873 1102 80-81 0.6195983202046484 1102 82-83 0.5639028627514691 1102 84-85 0.6326541096989295 1102 86-87 0.6550237239478172 1102 88-89 0.7890864030583771 1102 90-91 0.9059889282891866 1102 92-93 1.0535267932193584 1102 94-95 1.0535796129487771 1102 96-97 1.0252276687774184 1102 98-99 1.1728254523982748 1102 100-101 1.5326560506695586 1103 1 0.26934587920861475 1103 2 0.2968901217746094 1103 3 0.2893350194689077 1103 4 0.2257426071763149 1103 5 0.2040642120887739 1103 6 0.18719996726036925 1103 7 0.16746147821047686 1103 8 0.26742078096074096 1103 9 0.3278198702073638 1103 10-11 0.22638005157774188 1103 12-13 0.35576201867554147 1103 14-15 0.39542067243718293 1103 16-17 0.5009545680263088 1103 18-19 0.36838606684062114 1103 20-21 0.41663033425859197 1103 22-23 0.3847703103449476 1103 24-25 0.5015516692232893 1103 26-27 0.3080410254301711 1103 28-29 0.3064087605013839 1103 30-31 0.3500466534744291 1103 32-33 0.3534058570450398 1103 34-35 0.25226521159000725 1103 36-37 0.4168960704542073 1103 38-39 0.3612764792020826 1103 40-41 0.19013056729569655 1103 42-43 0.25891667217038616 1103 44-45 0.02060930128808991 1103 46-47 0.40209918380305965 1103 48-49 0.2509444913679957 1103 50-51 0.05886625576474813 1103 52-53 0.22027183386435922 1103 54-55 0.4628896269186882 1103 56-57 0.7638542496908798 1103 58-59 0.7917301660158529 1103 60-61 0.6458215766066999 1103 62-63 0.37465855764026657 1103 64-65 0.5250104801389668 1103 66-67 0.6023173393714742 1103 68-69 0.7662510850829705 1103 70-71 0.7753151013898076 1103 72-73 0.6254549903074391 1103 74-75 0.6371434584611286 1103 76-77 0.6479015031586073 1103 78-79 0.6433004417299557 1103 80-81 0.8000449742479177 1103 82-83 0.6247856412544159 1103 84-85 0.5683092767289857 1103 86-87 0.5654528226336168 1103 88-89 0.6157236171484115 1103 90-91 0.7745347927952295 1103 92-93 0.8025101437691653 1103 94-95 0.8722034508367607 1103 96-97 0.9653365773497704 1103 98-99 1.5155924454215182 1103 100-101 2.0290955082870745 1104 1 0.08959956312607176 1104 2 0.1685298468770995 1104 3 0.23859147287621596 1104 4 0.15662709679325815 1104 5 0.30321803543883163 1104 6 0.26196379413850934 1104 7 0.3020900205255117 1104 8 0.39312315717626944 1104 9 0.5362022829887252 1104 10-11 0.38712994421351965 1104 12-13 0.38399571086652884 1104 14-15 0.46511611872858083 1104 16-17 0.50439755001279 1104 18-19 0.5607280948917079 1104 20-21 0.7473690790495979 1104 22-23 0.5174110645659766 1104 24-25 0.5866630405885402 1104 26-27 0.44279693948017496 1104 28-29 0.6214221683490706 1104 30-31 0.61403584603908 1104 32-33 0.6275763345312413 1104 34-35 0.6424499231493925 1104 36-37 0.6235753089741252 1104 38-39 0.723092800142787 1104 40-41 0.7487632711721943 1104 42-43 0.6981293570425748 1104 44-45 0.5533207299142688 1104 46-47 0.42565844603891634 1104 48-49 0.37708703273845146 1104 50-51 0.18542529143672226 1104 52-53 0.12567183183542596 1104 54-55 0.1809398925394774 1104 56-57 0.43509371193417934 1104 58-59 0.5597129697044565 1104 60-61 0.5476157145762173 1104 62-63 0.49075789406735026 1104 64-65 0.22564914230742517 1104 66-67 0.5869667909403447 1104 68-69 0.5131352681060051 1104 70-71 0.5034102627292185 1104 72-73 0.5602654600386217 1104 74-75 0.5251374888973395 1104 76-77 0.6569262978139534 1104 78-79 0.5476081988940535 1104 80-81 0.6725903912889066 1104 82-83 0.8521503876888517 1104 84-85 0.7252228003384111 1104 86-87 0.5987768754895022 1104 88-89 0.75284548935511 1104 90-91 0.9557334848115033 1104 92-93 1.133532375209242 1104 94-95 0.7938100931036622 1104 96-97 0.6695568650746324 1104 98-99 1.0529112365848512 1104 100-101 1.1010317953671844 1105 1 0.1546738607729239 1105 2 0.31919514567022844 1105 3 0.3293581865227111 1105 4 0.3765206993718806 1105 5 0.30503191413009745 1105 6 0.29120559004636704 1105 7 0.3572831580177933 1105 8 0.38242463193242315 1105 9 0.26139826166445346 1105 10-11 0.4282144365304319 1105 12-13 0.40107430247930154 1105 14-15 0.44147131424539054 1105 16-17 0.5381467504925723 1105 18-19 0.49824585228822826 1105 20-21 0.6350618561454553 1105 22-23 0.6529565088052962 1105 24-25 0.5416617739525691 1105 26-27 0.4675943127151072 1105 28-29 0.4433282525404678 1105 30-31 0.25730007190056625 1105 32-33 0.2261444060674762 1105 34-35 0.5732048241908316 1105 36-37 0.5609817983490757 1105 38-39 0.47775031157400605 1105 40-41 0.4523823510486338 1105 42-43 0.4160271332217036 1105 44-45 0.29968352524688413 1105 46-47 0.41313904411503444 1105 48-49 0.45108726507167063 1105 50-51 0.4160356140300081 1105 52-53 0.46478527063311503 1105 54-55 0.45781111974877575 1105 56-57 0.6480257152764182 1105 58-59 0.6500395098568106 1105 60-61 0.5855707063115929 1105 62-63 0.6885329535152636 1105 64-65 0.864533326992742 1105 66-67 0.5704179470780701 1105 68-69 0.7548891880294235 1105 70-71 0.8646870317857918 1105 72-73 0.7070349784790224 1105 74-75 0.8721096690927475 1105 76-77 0.8962659116089498 1105 78-79 0.8146570490545173 1105 80-81 1.0512403672858035 1105 82-83 0.9638480857704224 1105 84-85 1.0739773682755853 1105 86-87 0.9644826716436903 1105 88-89 0.8910025830063937 1105 90-91 0.7712336100755017 1105 92-93 0.9686737555765248 1105 94-95 1.0365542284391651 1105 96-97 0.6953479527160411 1105 98-99 1.349102511212525 1105 100-101 1.267520246586308 1106 1 0.04899556997911603 1106 2 0.07126041444441711 1106 3 0.18052164605865073 1106 4 0.06767748128809359 1106 5 0.11322555187914674 1106 6 0.0560376743562756 1106 7 0.1030823732321906 1106 8 0.15219385736159552 1106 9 0.039995498716649536 1106 10-11 -0.1436889826092127 1106 12-13 -0.22109142810849391 1106 14-15 -0.23430462847252898 1106 16-17 -0.19480713756804136 1106 18-19 -0.2381203986249787 1106 20-21 0.02679350839353134 1106 22-23 -0.13147455468828895 1106 24-25 -0.19918459781894882 1106 26-27 -0.017995086398293836 1106 28-29 0.27086467366687117 1106 30-31 0.2883997634738691 1106 32-33 0.2585936710334522 1106 34-35 -0.015560411195266965 1106 36-37 0.07808410807823662 1106 38-39 -0.13637335000951367 1106 40-41 -0.05852828454837322 1106 42-43 0.002256856563292331 1106 44-45 -0.29495520560867305 1106 46-47 -0.23406362825515714 1106 48-49 -0.18171389723084985 1106 50-51 -0.26701876890172827 1106 52-53 0.08776173698220902 1106 54-55 0.22035479700156202 1106 56-57 0.12147556536054793 1106 58-59 0.13405041222802794 1106 60-61 -0.23746658588056135 1106 62-63 -0.022150907471235826 1106 64-65 0.15428251956273442 1106 66-67 0.29164219939953995 1106 68-69 0.42894200734030363 1106 70-71 0.3161139597832019 1106 72-73 0.24078175123829482 1106 74-75 0.28108825773484725 1106 76-77 0.3936956891648755 1106 78-79 0.2417453754512806 1106 80-81 0.0014202002130794256 1106 82-83 -0.06379709460795269 1106 84-85 0.15251873811324046 1106 86-87 -0.20729744293368668 1106 88-89 0.05543318369464956 1106 90-91 0.4864215586247731 1106 92-93 0.3684127664293122 1106 94-95 0.20561288240292797 1106 96-97 0.3332151804790726 1106 98-99 0.6743877149956248 1106 100-101 0.4752272775909532 1107 1 0.4433577788751677 1107 2 0.441777105393534 1107 3 0.4136674184004079 1107 4 0.29053099645815195 1107 5 0.38836017104051024 1107 6 0.4169951488596979 1107 7 0.5337746285769924 1107 8 0.34092329910883734 1107 9 0.5173991187046809 1107 10-11 0.6044076234807321 1107 12-13 0.6066074634243179 1107 14-15 0.8224603554736447 1107 16-17 0.8702519395770736 1107 18-19 0.771584969242717 1107 20-21 0.8622909484600356 1107 22-23 0.8478964004569249 1107 24-25 0.6749367809370312 1107 26-27 0.8541509645446155 1107 28-29 0.8363736231578542 1107 30-31 0.611968735526979 1107 32-33 0.6293524252752647 1107 34-35 0.9774368804361941 1107 36-37 0.9741613125669133 1107 38-39 0.9485006628974801 1107 40-41 0.9102067292218337 1107 42-43 0.854012500520156 1107 44-45 0.8997618772089382 1107 46-47 0.7874240562039176 1107 48-49 0.5892839516029085 1107 50-51 0.3833893132719055 1107 52-53 0.4747147538672607 1107 54-55 0.5986209387410426 1107 56-57 0.6653986349057917 1107 58-59 0.4126435677742464 1107 60-61 0.6874492628709916 1107 62-63 0.5034689800835039 1107 64-65 0.7303634691815972 1107 66-67 0.8381274860618433 1107 68-69 0.8985904674980603 1107 70-71 0.8991188330196778 1107 72-73 0.7797311052033464 1107 74-75 0.6548762285593703 1107 76-77 0.6978779034502622 1107 78-79 0.7500303341863379 1107 80-81 0.9057378329254249 1107 82-83 0.8675928054153417 1107 84-85 0.8113008400447193 1107 86-87 0.8082608326622243 1107 88-89 0.7616882361949813 1107 90-91 0.8437343820418839 1107 92-93 0.8941091551190148 1107 94-95 0.8766637111271613 1107 96-97 1.1105272274667541 1107 98-99 1.0625990042795301 1107 100-101 1.4045973192897847 1108 1 0.2676502856767655 1108 2 0.24577924122205985 1108 3 0.26268722566731384 1108 4 0.2623075481121475 1108 5 0.3216485841707666 1108 6 0.25473320474721106 1108 7 0.16221165977534469 1108 8 0.39617769154190086 1108 9 0.38738055733774956 1108 10-11 0.4088979486860538 1108 12-13 0.29828154616224367 1108 14-15 0.5983331211173208 1108 16-17 0.5952652885053453 1108 18-19 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#Quality Count 2 2637.0 3 366.0 4 381.0 5 384.0 6 483.0 7 575.0 8 671.0 9 873.0 10 1150.0 11 1672.0 12 2409.0 13 3393.0 14 4010.0 15 5265.0 16 7087.0 17 9436.0 18 8250.0 19 5274.0 20 4688.0 21 4741.0 22 4994.0 23 5684.0 24 6244.0 25 7388.0 26 8227.0 27 9733.0 28 11616.0 29 14274.0 30 17867.0 31 22130.0 32 28736.0 33 38202.0 34 53585.0 35 76147.0 36 113655.0 37 171100.0 38 158568.0 39 21963.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.81804402925018 20.239477163179604 15.294877608981478 22.647601198588735 2 14.202008056273673 20.660253712499248 42.75861239704202 22.379125834185054 3 18.20487077594551 25.152129085782647 31.281419270285383 25.36158086798646 4 11.494317833381631 17.5671597103063 40.197586206481915 30.740936249830153 5 12.314773952976063 37.95307707014871 36.236425574692255 13.495723402182971 6 29.48542780475554 37.658306565869594 19.288676431533574 13.567589197841299 7 26.255241940328993 33.45889958304796 22.769066243706668 17.51679223291638 8 24.066437406427458 35.671235049420716 21.88053474830638 18.38179279584544 9 25.02574640541726 16.806003689216553 22.693007913189252 35.47524199217694 10-11 23.13656544365458 27.21562911026336 29.731493337355097 19.916312108726963 12-13 24.255886486301364 25.039489582500934 29.62719070298967 21.07743322820803 14-15 21.203722118509592 26.71660094416882 28.59147964845724 23.48819728886435 16-17 19.97241298103813 29.51444563729198 29.57788616870255 20.93525521296734 18-19 19.535859249771097 28.35780184709442 32.21672959027956 19.889609312854923 20-21 21.12850178862668 27.24629584576061 32.037592697717585 19.58760966789513 22-23 20.09908026931666 27.734424986587637 31.743354947314796 20.423139796780912 24-25 19.869486097001076 27.420596132044693 32.390265647820335 20.319652123133896 26-27 20.069113100609307 27.6836898129068 32.0555259840987 20.191671102385193 28-29 19.61553366558819 28.14390901143666 31.98899293132298 20.25156439165217 30-31 20.112650162666835 27.667600912015715 32.16832037105461 20.05142855426283 32-33 19.699391236009603 27.650274340345455 32.06020827274934 20.590126150895603 34-35 20.131852788218442 27.873713074490276 32.01710853912926 19.977325598162015 36-37 19.981773366166106 27.79940129653003 31.801631265705755 20.41719407159811 38-39 19.850058806357094 28.00594190484543 31.911969466345823 20.232029822451654 40-41 20.396536075736854 27.8463038279883 31.66606299671375 20.091097099561093 42-43 19.950956127240136 27.972909327707264 31.477828132584257 20.598306412468343 44-45 20.32304787445264 28.074478713287355 31.200907374399474 20.401566037860533 46-47 20.21760195698784 28.116561406080383 30.73797352996127 20.927863106970506 48-49 20.30250376116935 28.18545809360385 30.804157233702 20.7078809115248 50-51 20.444839900412383 28.511340587720728 30.399516000346054 20.644303511520825 52-53 20.473083981630932 28.58021924136862 30.236487907592203 20.710208869408245 54-55 20.572988787129677 28.28815244457394 30.24267018301639 20.89618858527999 56-57 20.670316231553958 28.622355326827066 29.825342251822377 20.881986189796596 58-59 20.70251798582758 28.54942656190026 29.943241851941067 20.804813600331094 60-61 20.692490462324983 28.403892908966853 30.197672519003277 20.705944109704884 62-63 20.621705452431176 28.268960016145385 30.252896318756033 20.8564382126674 64-65 20.47925519933929 28.413244237555375 30.10616412643592 21.00133643666942 66-67 20.289423309670205 28.41449050601929 30.122764351188092 21.17332183312241 68-69 20.654454460106304 28.269554316728907 30.052791955008207 21.02319926815658 70-71 20.47462558723023 28.021986933019864 30.208967165563333 21.294420314186567 72-73 20.436804447690623 27.999644415399693 30.295868010840525 21.267683126069155 74-75 20.461313233680094 28.117687979195487 29.96247234385353 21.458526443270884 76-77 20.230626037303583 28.07181519642618 30.128191651354268 21.56936711491597 78-79 20.219473398401647 27.988745200370996 30.084062588603917 21.707718812623444 80-81 20.202677904792683 28.127250633556162 29.92036924355887 21.749702218092285 82-83 20.32968121926918 28.11923632919595 30.123845163816625 21.427237287718242 84-85 20.51076628154604 28.012811196404314 29.97214591743709 21.50427660461256 86-87 20.14770789703329 28.095310451441797 29.89154033859786 21.865441312927054 88-89 20.431082065595056 27.737326671363437 29.883607722167266 21.947983540874237 90-91 20.311518321462593 27.652536702389003 30.15290009237914 21.883044883769262 92-93 20.22711082974856 27.459916848557942 30.517492194792045 21.795480126901452 94-95 19.97645553730326 27.732854435758753 30.27175235069237 22.01893767624562 96-97 19.83042377238632 27.693446159908124 30.38797349440432 22.088156573301234 98-99 20.034492204113114 27.487619569370057 30.38179301794171 22.096095208575115 100-101 20.013802125094823 27.161296871218006 30.708827413975463 22.11607358971171 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 916.0 1 526.0 2 116.0 3 92.0 4 83.5 5 77.0 6 104.0 7 174.5 8 286.5 9 380.0 10 403.0 11 437.5 12 501.0 13 567.0 14 635.5 15 709.0 16 822.5 17 965.5 18 1117.0 19 1331.0 20 1610.0 21 1962.0 22 2434.0 23 3080.5 24 3990.5 25 5220.5 26 6765.5 27 8643.0 28 10419.5 29 12484.5 30 14873.0 31 17447.5 32 20609.5 33 23612.5 34 26485.0 35 29059.5 36 31122.5 37 32604.5 38 33178.0 39 33247.5 40 33126.5 41 32983.5 42 32820.5 43 32435.5 44 32111.5 45 31610.0 46 31004.0 47 31049.5 48 31888.5 49 30655.5 50 27674.5 51 24917.0 52 21983.5 53 19401.5 54 17038.5 55 14872.5 56 12695.5 57 10553.0 58 8644.5 59 6945.5 60 5349.0 61 4102.0 62 3096.0 63 2205.0 64 1544.0 65 1117.5 66 858.0 67 646.5 68 450.5 69 310.0 70 244.0 71 212.5 72 164.5 73 135.0 74 108.5 75 79.5 76 59.5 77 37.5 78 24.0 79 17.5 80 15.0 81 10.5 82 6.5 83 5.0 84 3.5 85 3.5 86 2.0 87 0.0 88 0.5 89 1.5 90 1.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30556724817984815 2 0.26491289294712894 3 0.2595162971197768 4 0.26647190951947514 5 0.26863054785041596 6 0.2029120031084392 7 0.19535676895014625 8 0.193917676729519 9 0.20435109532906645 10-11 0.2062099227807099 12-13 0.20117310000851463 14-15 0.20369151139461228 16-17 0.18990021094693466 18-19 0.19583646635702198 20-21 0.181865279381766 22-23 0.19241862233303234 24-25 0.19313816844334594 26-27 0.19187896275029712 28-29 0.19985393213960637 30-31 0.19625620158803828 32-33 0.2015928352395309 34-35 0.19703570987421132 36-37 0.18714195085739915 38-39 0.17580909961995975 40-41 0.1739502721683162 42-43 0.1850432747023178 44-45 0.1879814213194317 46-47 0.18702202650568023 48-49 0.20039359172234153 50-51 0.19511692024670838 52-53 0.21682322790783573 54-55 0.18666225345052342 56-57 0.17161174730979697 58-59 0.17598898614753813 60-61 0.16441628620666085 62-63 0.1706523524960455 64-65 0.1704724659684671 66-67 0.1691532980995588 68-69 0.17275102865112688 70-71 0.1745498939269109 72-73 0.17113204990292125 74-75 0.1846235394713015 76-77 0.17658860790613282 78-79 0.18024630063356034 80-81 0.17442996957519197 82-83 0.15919957690688713 84-85 0.15662120334493002 86-87 0.1603988204240765 88-89 0.16459617273423927 90-91 0.1702925794408887 92-93 0.16813394110994784 94-95 0.16687473541689904 96-97 0.17161174730979697 98-99 0.17952675452324673 100-101 0.16531571884455287 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 833859.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.75080918839757 #Duplication Level Percentage of deduplicated Percentage of total 1 85.41489787214545 67.26492324175791 2 10.516811402843567 16.564148161113952 3 2.482478576171083 5.864915899990015 4 0.8034191988094637 2.530796480949573 5 0.322570228405131 1.2701333253495146 6 0.14577120855897022 0.6887760378233742 7 0.08188661372930862 0.451404596501656 8 0.0458080706997639 0.28859381079725854 9 0.030208395760080744 0.21410420493507448 >10 0.13797695013254657 1.9138382765115005 >50 0.010384629515646561 0.5741187320755128 >100 0.0067180695544520695 0.9324566001413783 >500 4.5805014625037796E-4 0.22520839411523308 >1k 6.107335283338373E-4 1.216582237938029 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3939 0.47238202142088775 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3116 0.3736842799562036 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1978 0.2372103677000548 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1085 0.13011792161504523 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.199243517189357E-4 0.0 10-11 0.0 0.0 0.0 1.199243517189357E-4 0.0 12-13 0.0 0.0 0.0 1.199243517189357E-4 0.0 14-15 0.0 0.0 0.0 1.7988652757840355E-4 0.0 16-17 0.0 0.0 0.0 3.597730551568071E-4 0.0 18-19 0.0 0.0 0.0 3.597730551568071E-4 0.0 20-21 0.0 0.0 0.0 5.396595827352106E-4 0.0 22-23 0.0 0.0 0.0 8.3947046203255E-4 0.0 24-25 0.0 0.0 0.0 0.0010793191654704212 0.0 26-27 0.0 0.0 0.0 0.0016189787482056319 0.0 28-29 0.0 0.0 0.0 0.003597730551568071 0.0 30-31 0.0 0.0 0.0 0.008454666796184966 0.0 32-33 0.0 0.0 0.0 0.01840838798885663 0.0 34-35 0.0 0.0 0.0 0.027222827840198402 0.0 36-37 0.0 0.0 0.0 0.03297919672270731 0.0 38-39 0.0 0.0 0.0 0.03777617079146474 0.0 40-41 0.0 0.0 0.0 0.04347257749811419 0.0 42-43 0.0 0.0 0.0 0.049468795084060976 0.0 44-45 0.0 0.0 0.0 0.0574437644733702 0.0 46-47 0.0 0.0 0.0 0.06979597270042057 0.0 48-49 0.0 0.0 0.0 0.08592579800661743 0.0 50-51 0.0 0.0 0.0 0.10367460206101992 0.0 52-53 0.0 0.0 0.0 0.14720714173499355 0.0 54-55 0.0 0.0 0.0 0.21124674555290524 0.0 56-57 0.0 0.0 0.0 0.2913562125011543 0.0 58-59 0.0 0.0 0.0 0.3843575472591889 0.0 60-61 0.0 0.0 0.0 0.5135760362363422 0.0 62-63 0.0 0.0 0.0 0.6065773709943767 0.0 64-65 0.0 0.0 0.0 0.702217041490228 0.0 66-67 0.0 0.0 0.0 0.8741285996793222 0.0 68-69 0.0 0.0 0.0 1.0830967825495676 0.0 70-71 0.0 0.0 0.0 1.266820889382977 0.0 72-73 0.0 0.0 0.0 1.442629989002937 0.0 74-75 0.0 0.0 0.0 1.7637274407303873 0.0 76-77 0.0 0.0 0.0 2.021564796926099 0.0 78-79 0.0 0.0 0.0 2.2162020197659316 0.0 80-81 0.0 0.0 0.0 2.400285899654498 0.0 82-83 0.0 0.0 0.0 2.7139480415753745 0.0 84-85 0.0 0.0 0.0 3.0793575412629712 0.0 86-87 0.0 0.0 0.0 3.534290569508754 0.0 88-89 0.0 0.0 0.0 4.200110570252285 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4430 0.0 31.875734 1 GACTGCG 65 0.0058434117 29.230509 7 GGTATCA 2980 0.0 27.442245 1 TCAACGC 5145 0.0 27.350266 4 CAACGCA 5165 0.0 27.150686 5 GATCGGT 35 0.008385619 27.147509 30-31 ATCAACG 5185 0.0 27.13601 3 GTACATG 2985 0.0 27.077717 1 TATCAAC 5240 0.0 26.941896 2 AACGCAG 5225 0.0 26.817945 6 TACATGG 3060 0.0 26.097015 2 ACATGGG 3145 0.0 24.787128 3 CGCAGAG 5790 0.0 24.120407 8 ACGCAGA 5790 0.0 24.118958 7 GTATAGC 660 0.0 23.05226 1 CATTCCG 105 0.002195062 22.620207 9 GCAGAGT 6135 0.0 22.454287 9 AGTACTT 3380 0.0 22.27421 12-13 GAGTACT 3370 0.0 22.269833 12-13 AGAGTAC 4990 0.0 21.37005 10-11 >>END_MODULE