FastQCFastQC Report
Thu 26 May 2016
SRR937710_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937710_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833859
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46500.557648235493051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39630.4752602058621422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25360.30412815595922094No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10300.12352208227050378No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9310.11164957145032912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33150.031.6807251
GTATCAA49050.031.0994281
ATCAACG56650.027.0852413
TCAACGC56900.026.9662364
CAACGCA57150.026.9313955
TATCAAC57600.026.5560472
AACGCAG58500.026.3067466
GTATAGC4800.025.7405871
GTGTAGC5900.024.9687061
TAGCCCT9200.024.2683414
GAGTACT39650.023.42111812-13
ACGCAGA65750.023.4074047
CGCAGAG66100.023.2834648
TACATGG31850.022.9689622
TAGGACG2103.4402547E-822.6209474
GTACATG32650.022.559781
GTAGCCC5700.022.5018883
GTACTTT41900.022.1054114-15
GCAGAGT69200.021.8972029
ACATGGG33100.021.2404543
Produced by FastQC (version 0.11.5)