##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937710_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 833859 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.42387142190706 33.0 31.0 34.0 31.0 34.0 2 32.63813066717515 34.0 31.0 34.0 31.0 34.0 3 32.723130649186494 34.0 31.0 34.0 31.0 34.0 4 36.28307063904089 37.0 35.0 37.0 35.0 37.0 5 36.22436646963096 37.0 35.0 37.0 35.0 37.0 6 36.26304327230383 37.0 37.0 37.0 35.0 37.0 7 36.22103736962724 37.0 37.0 37.0 35.0 37.0 8 36.203283768598766 37.0 36.0 37.0 35.0 37.0 9 37.92495014145077 39.0 38.0 39.0 35.0 39.0 10-11 37.865247601812776 39.0 38.0 39.0 35.0 39.0 12-13 37.78850321217376 39.0 38.0 39.0 35.0 39.0 14-15 39.07419479792147 40.0 38.0 41.0 36.0 41.0 16-17 39.04968645778243 40.0 38.0 41.0 36.0 41.0 18-19 39.055997476791646 40.0 38.0 41.0 36.0 41.0 20-21 38.9692987663382 40.0 38.0 41.0 35.5 41.0 22-23 38.81927759969011 40.0 38.0 41.0 35.0 41.0 24-25 38.90702864632989 40.0 38.0 41.0 35.0 41.0 26-27 38.80342479963639 40.0 38.0 41.0 35.0 41.0 28-29 38.67477834981693 40.0 38.0 41.0 35.0 41.0 30-31 38.693604074549775 40.0 38.0 41.0 35.0 41.0 32-33 38.60285491911702 40.0 38.0 41.0 35.0 41.0 34-35 38.47644865618767 40.0 38.0 41.0 35.0 41.0 36-37 38.330762754854234 40.0 38.0 41.0 34.0 41.0 38-39 38.18590073381711 40.0 38.0 41.0 34.0 41.0 40-41 38.00941705971873 40.0 38.0 41.0 34.0 41.0 42-43 37.78318816490558 40.0 38.0 41.0 33.0 41.0 44-45 37.434015822818964 40.0 37.0 41.0 33.0 41.0 46-47 37.02334147619681 40.0 37.0 41.0 32.0 41.0 48-49 36.66051994401931 40.0 37.0 41.0 31.0 41.0 50-51 36.399878156858655 40.0 37.0 41.0 30.0 41.0 52-53 36.173469375517925 40.0 36.0 41.0 30.0 41.0 54-55 35.96594088449007 39.0 36.0 41.0 29.0 41.0 56-57 35.71823833525812 39.0 36.0 40.5 28.5 41.0 58-59 35.49388685617113 39.0 35.0 40.0 28.0 41.0 60-61 35.19244740417744 39.0 35.0 40.0 27.5 41.0 62-63 34.88614382047804 38.0 35.0 40.0 26.0 41.0 64-65 34.55338372554593 38.0 34.0 40.0 26.0 41.0 66-67 34.1730946119188 37.5 34.0 40.0 25.5 41.0 68-69 33.77613361491571 37.0 34.0 39.5 24.5 41.0 70-71 33.38670146871354 36.5 33.0 39.0 23.5 40.0 72-73 32.89458949294785 36.0 33.0 39.0 22.0 40.0 74-75 32.39970606541394 35.5 32.0 38.0 20.5 40.0 76-77 31.117633796601105 34.0 30.5 36.5 20.0 39.0 78-79 31.681864679760007 35.0 32.0 37.0 20.0 39.0 80-81 31.457878370324 35.0 32.0 36.5 18.5 39.0 82-83 31.08277178755641 35.0 31.0 36.0 18.0 37.5 84-85 30.67057440166743 34.0 31.0 36.0 13.0 37.0 86-87 30.248537822341667 34.0 31.0 35.0 7.5 37.0 88-89 29.877520659967693 34.0 30.5 35.0 4.5 36.0 90-91 29.38050857519077 34.0 30.0 35.0 2.0 36.0 92-93 28.95095873522982 34.0 29.0 35.0 2.0 35.5 94-95 28.518531910071125 34.0 29.0 35.0 2.0 35.0 96-97 28.042395057197922 34.0 29.0 35.0 2.0 35.0 98-99 27.26463107072059 33.5 27.5 35.0 2.0 35.0 100-101 24.686369638032332 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2592174589599878 1101 2 0.22393125196748542 1101 3 0.11621608433512876 1101 4 0.13611597206800496 1101 5 0.10604226207988887 1101 6 0.13430701640345433 1101 7 0.14386627997462398 1101 8 -0.029287169601900587 1101 9 0.13959085613399225 1101 10-11 0.2298088722195999 1101 12-13 0.27806504152954403 1101 14-15 0.2648954607818439 1101 16-17 0.17138556968350827 1101 18-19 0.2600335836515839 1101 20-21 0.3109333873916924 1101 22-23 0.22504053339348928 1101 24-25 0.2737384710505424 1101 26-27 0.2514521524886746 1101 28-29 0.30181451856957864 1101 30-31 0.33152274564002226 1101 32-33 0.22427492120780101 1101 34-35 0.16681163430813228 1101 36-37 0.19950081365852412 1101 38-39 0.15282806258507975 1101 40-41 0.10143879619434415 1101 42-43 0.17876379151352495 1101 44-45 0.2236948324600263 1101 46-47 0.1729647199322244 1101 48-49 0.028271643811009994 1101 50-51 0.189144245004627 1101 52-53 0.028597372497252138 1101 54-55 0.3214596806071057 1101 56-57 0.09489722532295275 1101 58-59 -0.1032038240152815 1101 60-61 -0.15616167015402738 1101 62-63 -0.2233325201114127 1101 64-65 0.035015604631666974 1101 66-67 0.05348233613649711 1101 68-69 0.03579745402324619 1101 70-71 0.18565966130829992 1101 72-73 0.10548785074575306 1101 74-75 0.13095733001512144 1101 76-77 0.03813058037245298 1101 78-79 0.06530556222106654 1101 80-81 0.1318913860354769 1101 82-83 0.030279316535303025 1101 84-85 -0.045403772065530035 1101 86-87 -0.22588067427288294 1101 88-89 0.10224509620695343 1101 90-91 -0.12285981852471295 1101 92-93 0.16039608664909366 1101 94-95 0.09700383064426887 1101 96-97 0.30822842816908036 1101 98-99 0.42864061210269 1101 100-101 0.6515914776478731 1102 1 0.13332154544077923 1102 2 0.0752678991811564 1102 3 0.14605114500887595 1102 4 0.11904081501914021 1102 5 0.10913593045080461 1102 6 0.13932176666281038 1102 7 0.12273775841356382 1102 8 0.0522312454126137 1102 9 0.18127343325605239 1102 10-11 0.18610214725025997 1102 12-13 0.034068211230184886 1102 14-15 0.1861235001293835 1102 16-17 0.19584092522727303 1102 18-19 0.1300522031084128 1102 20-21 0.1788442235100618 1102 22-23 0.21494513824535488 1102 24-25 0.06915592197593412 1102 26-27 0.01203584373684663 1102 28-29 -0.07526834308210795 1102 30-31 0.061519501596727366 1102 32-33 -0.0063461441224959 1102 34-35 -0.062233873536705175 1102 36-37 0.0920401899236154 1102 38-39 0.10923707561870089 1102 40-41 0.17045750688988903 1102 42-43 0.2529239729096133 1102 44-45 0.0894184737634518 1102 46-47 0.06453679682956448 1102 48-49 0.060189988537921124 1102 50-51 0.09979788593634709 1102 52-53 0.13187453206700894 1102 54-55 0.08800261180297753 1102 56-57 -0.09985773113590568 1102 58-59 -0.2253750013664515 1102 60-61 -0.0943051987945367 1102 62-63 0.01995552052022731 1102 64-65 -0.12973702017106348 1102 66-67 0.20857294969997753 1102 68-69 -0.04150877352611104 1102 70-71 0.04672421439065033 1102 72-73 0.008999527612004954 1102 74-75 -0.130770224415528 1102 76-77 -0.0749910678718777 1102 78-79 -0.07221990858760918 1102 80-81 -0.12931154576864756 1102 82-83 -0.048392112567146484 1102 84-85 -9.530071955765607E-5 1102 86-87 0.057649554465964314 1102 88-89 0.08814111110251943 1102 90-91 0.21740399653778653 1102 92-93 0.225312434261852 1102 94-95 0.1932885562691702 1102 96-97 0.12495265407614653 1102 98-99 0.2981396777506333 1102 100-101 0.8917064384321236 1103 1 0.2456259085008199 1103 2 0.2281374830266003 1103 3 0.19318010880707703 1103 4 0.11650463772999586 1103 5 0.06393406199434537 1103 6 0.11664231441901052 1103 7 0.14358451847884623 1103 8 0.13691723689090907 1103 9 0.2443529007495968 1103 10-11 0.17975398838426315 1103 12-13 0.2137187924450501 1103 14-15 0.2961549254900646 1103 16-17 0.2707266883225401 1103 18-19 0.19647617497626158 1103 20-21 0.20644943311231145 1103 22-23 0.16970514543451998 1103 24-25 0.2795311019319655 1103 26-27 0.26449486459450355 1103 28-29 0.13453951017179833 1103 30-31 0.1684365302934765 1103 32-33 0.38973821236563566 1103 34-35 0.3353554872267992 1103 36-37 0.21912545594991428 1103 38-39 0.22227034002562363 1103 40-41 0.22962605102972589 1103 42-43 0.3291409113787225 1103 44-45 0.25111747653926386 1103 46-47 0.1575733835016564 1103 48-49 0.11541992378098342 1103 50-51 0.18905063043600023 1103 52-53 0.0910029548624749 1103 54-55 0.1212488642697096 1103 56-57 0.014968739511061813 1103 58-59 0.04398627046200687 1103 60-61 0.08594992575410032 1103 62-63 0.07683433409032148 1103 64-65 0.1654734716512607 1103 66-67 0.15757164076380548 1103 68-69 0.24541148666353507 1103 70-71 0.22373922267815516 1103 72-73 0.27926035265934246 1103 74-75 0.17235470183199908 1103 76-77 0.12660552562628524 1103 78-79 0.21781837915686353 1103 80-81 0.31558060769458507 1103 82-83 0.3261242981519743 1103 84-85 0.2889590155927628 1103 86-87 0.2768373604819878 1103 88-89 0.140289781561048 1103 90-91 0.15725057324307912 1103 92-93 0.2831711345118393 1103 94-95 0.4438540675258409 1103 96-97 0.628509565567736 1103 98-99 0.5281193992249946 1103 100-101 0.6038893481917746 1104 1 0.29267905632980984 1104 2 0.2108002736849528 1104 3 0.035384564112561634 1104 4 0.0014890882336828781 1104 5 0.04538400321172986 1104 6 0.041920193330192035 1104 7 0.10858772531589267 1104 8 0.05069671696625022 1104 9 0.04389007920641319 1104 10-11 0.1493933009375965 1104 12-13 0.17221364178006127 1104 14-15 0.26952859696341136 1104 16-17 0.2015987789210385 1104 18-19 0.10349988077235395 1104 20-21 0.21484131423502362 1104 22-23 0.1049986132934464 1104 24-25 0.07609138877761978 1104 26-27 0.07104256080051385 1104 28-29 -0.022129978210877255 1104 30-31 0.1987752376619838 1104 32-33 0.12923032602630968 1104 34-35 0.1719414963998247 1104 36-37 0.23373713559985276 1104 38-39 0.10278043564459693 1104 40-41 0.22149173133568922 1104 42-43 0.34227316269104335 1104 44-45 0.6000252920914662 1104 46-47 0.499028825374495 1104 48-49 0.3802719753113024 1104 50-51 0.284180972299481 1104 52-53 0.24142108358006453 1104 54-55 0.22935872408189795 1104 56-57 0.4761823554368192 1104 58-59 0.3954916803791946 1104 60-61 0.5541233361717488 1104 62-63 0.4941683873181759 1104 64-65 0.6066778897596876 1104 66-67 0.4794665038504675 1104 68-69 0.5745011620008711 1104 70-71 0.47118474308187075 1104 72-73 0.366952410637154 1104 74-75 0.4515039488170629 1104 76-77 0.4379860210685216 1104 78-79 0.6797612828040904 1104 80-81 0.6862763166197361 1104 82-83 0.5820552559085179 1104 84-85 0.41287649674343996 1104 86-87 0.4115487507839255 1104 88-89 0.32253280908573956 1104 90-91 0.16001273639838232 1104 92-93 0.3941536214618715 1104 94-95 0.3972371397095458 1104 96-97 0.5279354910135652 1104 98-99 0.8582539736624994 1104 100-101 1.0695976083051484 1105 1 0.19703754370053872 1105 2 0.08909965044406931 1105 3 0.14382399861908368 1105 4 0.08025285152810824 1105 5 0.040260075828456365 1105 6 0.07381142778940841 1105 7 -0.021458699341664556 1105 8 -0.026727631276060038 1105 9 0.17536969196570595 1105 10-11 0.17914175123297582 1105 12-13 0.08973954483552404 1105 14-15 0.07380814702072769 1105 16-17 0.06807925243295188 1105 18-19 0.03778736654894743 1105 20-21 0.2330976673591394 1105 22-23 0.22257672134519169 1105 24-25 0.2218895863756316 1105 26-27 0.13680056195098445 1105 28-29 0.07783839087984035 1105 30-31 -0.009574482628401881 1105 32-33 -0.02786752405157955 1105 34-35 -0.022097336839507875 1105 36-37 -0.05684105222896818 1105 38-39 -0.06849394009981324 1105 40-41 -0.08210551481576545 1105 42-43 -0.11167657215516158 1105 44-45 -0.042288606893961855 1105 46-47 -0.24898759470637088 1105 48-49 -0.0014200264074020197 1105 50-51 0.06551831594691748 1105 52-53 0.07103545241045595 1105 54-55 -0.03802952853429531 1105 56-57 -0.02426831504048721 1105 58-59 0.20660686587488186 1105 60-61 0.2221947324524649 1105 62-63 0.06339389190815581 1105 64-65 -0.025879413271418628 1105 66-67 -0.10921131014578833 1105 68-69 -0.14656110470367878 1105 70-71 0.05323546170533433 1105 72-73 0.028964948233848986 1105 74-75 -0.15008305507801367 1105 76-77 -0.19151106146995645 1105 78-79 0.06815455857663366 1105 80-81 0.041249997183943066 1105 82-83 0.2497648561694028 1105 84-85 -0.08514727122564736 1105 86-87 0.03071190329389495 1105 88-89 0.02682019689048687 1105 90-91 -0.08051233669561242 1105 92-93 0.17108153238027413 1105 94-95 0.17197118114603427 1105 96-97 0.35559812077377373 1105 98-99 0.5448175552891428 1105 100-101 0.358656532770258 1106 1 0.04533491595800143 1106 2 0.12052082643241135 1106 3 0.1310852819782582 1106 4 0.12411357357053276 1106 5 0.12110224929450908 1106 6 0.16029652049923726 1106 7 0.08650551919501481 1106 8 0.09528107607560088 1106 9 0.10095720680027398 1106 10-11 0.007053375249931548 1106 12-13 -0.10784527250513065 1106 14-15 -0.032768481971380936 1106 16-17 0.14106861121616987 1106 18-19 0.254791826008848 1106 20-21 0.0477366957324179 1106 22-23 -0.14728903205229216 1106 24-25 0.095819762274715 1106 26-27 -0.0329977540987727 1106 28-29 0.16482017287869866 1106 30-31 0.06843879327299618 1106 32-33 0.07465625257211883 1106 34-35 -0.11385593096915159 1106 36-37 -0.2981750224705806 1106 38-39 -0.25698545919218674 1106 40-41 -0.225211533599051 1106 42-43 -0.3918456944448536 1106 44-45 -0.3377617096400485 1106 46-47 -0.04705653947716826 1106 48-49 -0.025646232428499616 1106 50-51 -0.14817791185908646 1106 52-53 -0.28250980168618867 1106 54-55 -0.20098411080289935 1106 56-57 -0.2204729807793413 1106 58-59 -0.31587472934896965 1106 60-61 -0.2785308801860893 1106 62-63 -0.042232333286307266 1106 64-65 -0.35613398925909223 1106 66-67 -0.2973697746899404 1106 68-69 -0.17191787802493508 1106 70-71 -0.19588107598189453 1106 72-73 -0.04581199612703557 1106 74-75 -0.16295896044093894 1106 76-77 -0.07159820110163295 1106 78-79 -0.06783086966705199 1106 80-81 0.23600426408377473 1106 82-83 -0.2158825919550793 1106 84-85 -0.18332480996080136 1106 86-87 -0.21327741251454668 1106 88-89 -0.15575319907583562 1106 90-91 0.07089194689826783 1106 92-93 -0.054104664120256274 1106 94-95 0.1346798154405704 1106 96-97 0.3023350126393076 1106 98-99 0.0620248226550828 1106 100-101 0.2940827930056926 1107 1 0.30328532495362026 1107 2 0.27768841187389626 1107 3 0.12663908168686788 1107 4 -0.06650898581489884 1107 5 0.08754222901599462 1107 6 0.1925640141393643 1107 7 0.1945091364427043 1107 8 0.1905215636732791 1107 9 0.2051266328362331 1107 10-11 0.23087400178280149 1107 12-13 0.22583669286611752 1107 14-15 0.3111589264442003 1107 16-17 0.4050157957460385 1107 18-19 0.2753398024967382 1107 20-21 0.32708116093238715 1107 22-23 0.327817270271062 1107 24-25 0.22399227575946412 1107 26-27 0.15585500291079057 1107 28-29 0.4339386057700736 1107 30-31 0.11635476533979983 1107 32-33 0.040462528862256875 1107 34-35 0.052460961851615195 1107 36-37 0.03668871416455488 1107 38-39 0.1835389047065874 1107 40-41 0.14037939770521035 1107 42-43 0.1588939687674582 1107 44-45 0.002325798523877154 1107 46-47 -0.14099422416460783 1107 48-49 0.01822888118139332 1107 50-51 0.09370611698754772 1107 52-53 -0.00486092134310212 1107 54-55 -0.18798005966817044 1107 56-57 -0.17481436309776655 1107 58-59 -0.03650057714139621 1107 60-61 0.005375107911710586 1107 62-63 -0.025259357597676058 1107 64-65 -0.04803537358709775 1107 66-67 -0.031869731301242155 1107 68-69 0.2259793683909308 1107 70-71 -0.05763781607442553 1107 72-73 0.07146196014628714 1107 74-75 -0.11395712898092825 1107 76-77 -0.09045173454061839 1107 78-79 -0.22612427778119937 1107 80-81 -0.3358508541846952 1107 82-83 -0.22654365310574676 1107 84-85 -0.10189106451090524 1107 86-87 -0.0199784552136002 1107 88-89 -0.1671712789201827 1107 90-91 -0.06516018348706965 1107 92-93 -0.37028956393802304 1107 94-95 -0.15676781439441356 1107 96-97 -0.6591438113898143 1107 98-99 -0.303802462901956 1107 100-101 0.45817087664084255 1108 1 0.24339821874701073 1108 2 0.273027728917306 1108 3 0.20334329486289704 1108 4 0.10579898031696189 1108 5 0.0972856753278819 1108 6 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-0.3598666752360984 2316 60-61 -0.6703303798888314 2316 62-63 -0.6136564978683978 2316 64-65 -0.7487046870952554 2316 66-67 -0.5783438960849452 2316 68-69 -0.28426882645842255 2316 70-71 -0.34446431490187734 2316 72-73 -0.37792076030305566 2316 74-75 -0.4273957894985614 2316 76-77 -0.5500719579302391 2316 78-79 -0.5630887022357314 2316 80-81 -0.49526543652308774 2316 82-83 -0.42610279005484486 2316 84-85 -0.3534146615542362 2316 86-87 -0.4347318641506064 2316 88-89 -0.3096142375852615 2316 90-91 -0.21842513612191183 2316 92-93 -0.18917931609956895 2316 94-95 -0.28037341125241966 2316 96-97 -0.040057328101266165 2316 98-99 -0.08881311917133417 2316 100-101 0.07449884738778323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 7.0 5 9.0 6 29.0 7 58.0 8 139.0 9 296.0 10 523.0 11 1015.0 12 1741.0 13 2496.0 14 3277.0 15 4829.0 16 7046.0 17 10777.0 18 9845.0 19 5399.0 20 3864.0 21 3405.0 22 3349.0 23 3643.0 24 4071.0 25 4766.0 26 5717.0 27 6805.0 28 8577.0 29 11136.0 30 14233.0 31 18254.0 32 23854.0 33 32710.0 34 46574.0 35 69558.0 36 112635.0 37 193802.0 38 197229.0 39 22187.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.66072923638649 21.07451894819922 15.457589955067446 21.80716186034684 2 14.85347043085222 21.858851436513845 41.231790986245876 22.05588714638806 3 18.620294318343987 25.8548507601405 31.212830946239112 24.312023975276396 4 12.087804023951348 18.51204702964657 39.22243262333949 30.177716323062587 5 12.521606245149467 38.790961902237214 35.44407805957026 13.243353793043058 6 29.623713361611497 38.64598211448219 18.33367511773573 13.396629406170588 7 26.449435695962986 34.27665828395448 21.953351825668367 17.320554194414164 8 24.421155135340626 36.63892816411408 20.87139432446013 18.068522376085166 9 25.41161035618732 17.18671861789583 22.058765330829313 35.34290569508754 10-11 23.46733680394407 28.12333979725589 28.65064717176405 19.758676227035984 12-13 24.828307431786197 25.753375536155133 28.91786760018973 20.500449431868947 14-15 21.45734470695885 27.52617648787145 28.093418671502018 22.923060133667683 16-17 20.10303900299691 30.437939747607206 28.9473400179167 20.511681231479184 18-19 19.700273067748864 28.865671534396103 31.983284943857416 19.450770453997617 20-21 21.18253805499491 27.755351924006337 31.881349244896317 19.180760776102435 22-23 20.282510030323326 28.224679242522456 31.63825716124011 19.85455356591411 24-25 20.10435817086582 28.021044325239643 32.15621585903612 19.718381644858425 26-27 20.22354073412264 28.166432312669208 31.994387496589628 19.615639456618528 28-29 19.90594192096507 28.672183669296448 31.817846570082143 19.60402783965634 30-31 20.394635064201502 28.23301061690286 31.942570626448834 19.429783692446804 32-33 20.14123490901939 28.27342512343214 31.72712652858577 19.8582134389627 34-35 20.49771004450393 28.48623088555739 31.639461827479227 19.376597242459457 36-37 20.2752503720653 28.35125602769773 31.608101609504725 19.765391990732244 38-39 20.258520925000507 28.56484129810915 31.642939633679074 19.53369814321126 40-41 20.763289386659377 28.422446922503585 31.366076573586543 19.44818711725049 42-43 20.32240943479395 28.607120474738295 31.192333180404752 19.878136910063002 44-45 20.689589007254224 28.729377508667532 30.77246872672718 19.808564757351064 46-47 20.697144241412516 28.841926512755755 30.259012614842558 20.20191663098917 48-49 20.774135675216073 28.814703684915553 30.249298742353325 20.16186189751505 50-51 20.966794146252543 29.367794795043288 29.536108622680814 20.129302436023355 52-53 21.059375745779562 29.454620025687795 29.239415776528165 20.246588452004474 54-55 21.072627386644506 29.265499323027033 29.25602529924124 20.40584799108722 56-57 21.25809039657784 29.402872667921077 28.932049663072533 20.40698727242855 58-59 21.19171226790141 29.43615167552308 28.964968897619382 20.40716715895613 60-61 21.232546509661706 29.26447996603742 29.337513896234256 20.165459628066614 62-63 21.150278404382515 29.119971122216104 29.370972790363837 20.35877768303754 64-65 21.09115569898508 29.30195632594959 29.170399312113922 20.43648866295141 66-67 20.936273398740074 29.22682371959768 29.150491869728572 20.68641101193367 68-69 21.160724511412905 29.26521706020866 29.126164691011724 20.447893737366712 70-71 21.099070706198532 29.08117559443503 29.128125978132996 20.691627721233445 72-73 21.139305326200233 28.965328670674538 29.175316210534397 20.720049792590835 74-75 21.037429589414998 29.237317100373083 28.838028971324885 20.88722433888703 76-77 20.880748423894207 28.993151120273332 29.21369200308445 20.912408452748007 78-79 20.72970370290421 29.04711707854685 28.985116188708165 21.238063029840777 80-81 20.759684790833944 29.191625922368168 28.969885796039858 21.07880349075803 82-83 20.8250134018356 29.273986287553623 29.04768415081532 20.85331615979545 84-85 20.874375264210396 29.22339382564662 28.916624942660725 20.98560596748226 86-87 20.482299765308042 29.25710461840671 28.777347249355106 21.48324836693014 88-89 20.82420409205873 29.069063234911418 28.66977510586322 21.43695756716663 90-91 20.800757531689257 28.946442053803224 28.91957582543188 21.333224589075638 92-93 20.53951823888736 28.828073609154792 29.247820166183658 21.38458798577419 94-95 20.334852774869614 29.049935300812248 28.927312651179637 21.687899273138502 96-97 20.33623190491438 29.04399904540216 29.101382847699668 21.518386201983787 98-99 20.44300799309224 28.978647110674043 28.839352637480587 21.738992258753125 100-101 20.04177819413366 29.47845628193264 28.721878735283024 21.757886788650676 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 71.0 1 49.0 2 37.0 3 47.0 4 38.0 5 40.0 6 77.0 7 143.5 8 246.0 9 363.0 10 390.0 11 417.5 12 502.0 13 583.5 14 673.5 15 801.5 16 956.0 17 1112.5 18 1307.5 19 1523.0 20 1822.5 21 2210.5 22 2733.0 23 3434.0 24 4309.0 25 5482.5 26 7002.0 27 8902.5 28 10845.0 29 12798.5 30 15033.0 31 17752.5 32 20664.0 33 23476.0 34 26199.0 35 28549.5 36 30776.0 37 32455.0 38 33109.0 39 33084.5 40 32854.5 41 32786.5 42 32716.0 43 32538.0 44 31863.0 45 31212.0 46 30948.0 47 31181.0 48 32308.0 49 31015.5 50 27705.0 51 24795.5 52 22020.0 53 19475.0 54 16970.0 55 14646.5 56 12396.5 57 10428.5 58 8624.5 59 6863.5 60 5238.5 61 4022.0 62 3079.5 63 2180.5 64 1500.5 65 1089.5 66 876.5 67 674.0 68 470.5 69 314.0 70 222.0 71 184.5 72 164.0 73 141.5 74 107.0 75 80.0 76 62.5 77 43.0 78 26.0 79 17.5 80 13.5 81 8.0 82 5.0 83 4.5 84 3.0 85 2.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019787518033624392 2 0.0 3 0.0 4 2.398487034378714E-4 5 0.022545778123159912 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003417844023989667 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014990543964866962 24-25 0.0 26-27 7.79508286173082E-4 28-29 0.0014990543964866962 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.994326378920178E-4 42-43 0.016369674009634724 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 5.996217585946785E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0021586383309408425 84-85 7.79508286173082E-4 86-87 0.0 88-89 0.0 90-91 0.011812548644315165 92-93 0.0026982979136760533 94-95 0.0 96-97 0.0 98-99 0.0016789409240651 100-101 0.020447101968078536 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 833859.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.34936210465621 #Duplication Level Percentage of deduplicated Percentage of total 1 83.98727446770792 64.12374830518178 2 11.214223513588038 17.123976235229662 3 2.8402902112168937 6.505630374555273 4 0.9529676689834858 2.9103389453300124 5 0.40596568626026897 1.5497610591175266 6 0.19218105049845993 0.8803740368496081 7 0.1128867332661532 0.6033181052464489 8 0.06403260124831316 0.3911078607368418 9 0.03979065240174121 0.27341918357409434 >10 0.17001912437603284 2.260775847303173 >50 0.011685122345520863 0.6066420422484161 >100 0.007735913444773257 1.1273839820069311 >500 3.157515541303121E-4 0.17550208851741494 >1k 6.315031082606242E-4 1.468021934102745 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4650 0.557648235493051 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3963 0.4752602058621422 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2536 0.30412815595922094 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1030 0.12352208227050378 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 931 0.11164957145032912 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.199243517189357E-4 0.0 6 0.0 0.0 0.0 1.199243517189357E-4 0.0 7 0.0 0.0 0.0 1.199243517189357E-4 0.0 8 0.0 0.0 0.0 1.199243517189357E-4 0.0 9 0.0 0.0 0.0 1.199243517189357E-4 0.0 10-11 0.0 0.0 0.0 1.199243517189357E-4 0.0 12-13 0.0 0.0 0.0 1.199243517189357E-4 0.0 14-15 0.0 0.0 0.0 1.7988652757840355E-4 0.0 16-17 0.0 0.0 0.0 2.398487034378714E-4 0.0 18-19 0.0 0.0 0.0 2.398487034378714E-4 0.0 20-21 0.0 0.0 0.0 4.19735231016275E-4 0.0 22-23 0.0 0.0 0.0 6.595839344541463E-4 0.0 24-25 0.0 0.0 0.0 8.394704620325498E-4 0.0 26-27 0.0 0.0 0.0 0.0013791300447677604 0.0 28-29 0.0 0.0 0.0 0.003597730551568071 0.0 30-31 0.0 0.0 0.0 0.008574591147903902 0.0 32-33 0.0 0.0 0.0 0.0186482366922945 0.0 34-35 0.0 0.0 0.0 0.027702525247074145 0.0 36-37 0.0 0.0 0.0 0.033518856305442525 0.0 38-39 0.0 0.0 0.0 0.03837579255005942 0.0 40-41 0.0 0.0 0.0 0.04437201013600621 0.0 42-43 0.0 0.0 0.0 0.050488152073671924 0.0 44-45 0.0 0.0 0.0 0.058702970166419025 0.0 46-47 0.0 0.0 0.0 0.07105517839346939 0.0 48-49 0.0 0.0 0.0 0.0880244741616988 0.0 50-51 0.0 0.0 0.0 0.10607308909539861 0.0 52-53 0.0 0.0 0.0 0.1505050614072643 0.0 54-55 0.0 0.0 0.0 0.21442474087345703 0.0 56-57 0.0 0.0 0.0 0.29477405652514393 0.0 58-59 0.0 0.0 0.0 0.3892744456796653 0.0 60-61 0.0 0.0 0.0 0.519332405118851 0.0 62-63 0.0 0.0 0.0 0.6124536642286046 0.0 64-65 0.0 0.0 0.0 0.7094125025933642 0.0 66-67 0.0 0.0 0.0 0.882403379947929 0.0 68-69 0.0 0.0 0.0 1.0929305793905204 0.0 70-71 0.0 0.0 0.0 1.277554118861822 0.0 72-73 0.0 0.0 0.0 1.455222045933425 0.0 74-75 0.0 0.0 0.0 1.7825555639502602 0.0 76-77 0.0 0.0 0.0 2.0421318232458967 0.0 78-79 0.0 0.0 0.0 2.2387477978890917 0.0 80-81 0.0 0.0 0.0 2.423851034767269 0.0 82-83 0.0 0.0 0.0 2.7416505668224485 0.0 84-85 0.0 0.0 0.0 3.110178099654738 0.0 86-87 0.0 0.0 0.0 3.571107345486467 0.0 88-89 0.0 0.0 0.0 4.249639327512206 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3315 0.0 31.680725 1 GTATCAA 4905 0.0 31.099428 1 ATCAACG 5665 0.0 27.085241 3 TCAACGC 5690 0.0 26.966236 4 CAACGCA 5715 0.0 26.931395 5 TATCAAC 5760 0.0 26.556047 2 AACGCAG 5850 0.0 26.306746 6 GTATAGC 480 0.0 25.740587 1 GTGTAGC 590 0.0 24.968706 1 TAGCCCT 920 0.0 24.268341 4 GAGTACT 3965 0.0 23.421118 12-13 ACGCAGA 6575 0.0 23.407404 7 CGCAGAG 6610 0.0 23.283464 8 TACATGG 3185 0.0 22.968962 2 TAGGACG 210 3.4402547E-8 22.620947 4 GTACATG 3265 0.0 22.55978 1 GTAGCCC 570 0.0 22.501888 3 GTACTTT 4190 0.0 22.10541 14-15 GCAGAGT 6920 0.0 21.897202 9 ACATGGG 3310 0.0 21.240454 3 >>END_MODULE