FastQCFastQC Report
Thu 26 May 2016
SRR937709_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937709_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772105
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39480.5113294176310217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29860.3867349648040098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19610.2539809999935242No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10640.13780509127644555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGCG156.616337E-495.002727
GGGCGTA500.001615257538.0010877
GGTATCA30000.030.5850221
CCGTAGG650.005842071529.2316079
GTATCAA43350.028.733261
GTATAGC5050.027.301051
GTACATG29350.026.5649171
TACATGG29350.025.5830572
ATCAACG49650.025.1716543
TCAACGC49650.025.1716544
CAACGCA50200.024.895875
TATCAAC51000.024.7864842
ACATGGG29700.024.6399353
AACGCAG51200.024.4001146
ATAGCCC5900.023.3572253
GAGTACT32100.022.93530712-13
ACGCAGA56000.022.1390297
CGCAGAG56050.022.1192788
GTCCTAC4300.022.1123181
CATGGGT9800.021.8203494
Produced by FastQC (version 0.11.5)