##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937709_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 772105 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03228964972381 33.0 31.0 34.0 31.0 34.0 2 32.178982133259076 33.0 31.0 34.0 31.0 34.0 3 32.208584324670866 34.0 31.0 34.0 30.0 34.0 4 35.78856891225934 37.0 35.0 37.0 35.0 37.0 5 35.78745118863367 37.0 35.0 37.0 35.0 37.0 6 35.83986504426211 37.0 35.0 37.0 35.0 37.0 7 35.79545010069874 37.0 35.0 37.0 35.0 37.0 8 35.7895817278738 37.0 35.0 37.0 35.0 37.0 9 37.4767253158573 39.0 37.0 39.0 35.0 39.0 10-11 37.3758271219588 39.0 37.0 39.0 35.0 39.0 12-13 37.275847196948604 39.0 37.0 39.0 34.5 39.0 14-15 38.49516646052027 40.0 38.0 41.0 34.0 41.0 16-17 38.43354336521587 40.0 38.0 41.0 34.0 41.0 18-19 38.41159881104254 40.0 38.0 41.0 34.0 41.0 20-21 38.352435225778876 40.0 38.0 41.0 34.0 41.0 22-23 38.165643273907044 40.0 38.0 41.0 34.0 41.0 24-25 38.02772679881622 40.0 38.0 41.0 33.5 41.0 26-27 37.99166887923275 40.0 38.0 41.0 33.5 41.0 28-29 38.03029898783197 40.0 38.0 41.0 34.0 41.0 30-31 37.87753867673438 40.0 37.5 41.0 33.5 41.0 32-33 37.64374858341806 40.0 37.0 41.0 33.0 41.0 34-35 37.67708342777213 40.0 37.0 41.0 33.0 41.0 36-37 37.603943116545025 40.0 37.0 41.0 33.0 41.0 38-39 37.478465364166794 40.0 37.0 41.0 33.0 41.0 40-41 37.27333134742037 40.0 37.0 41.0 32.5 41.0 42-43 37.03414043426736 40.0 37.0 41.0 31.5 41.0 44-45 36.776869726267805 40.0 37.0 41.0 31.0 41.0 46-47 36.4056540237403 40.0 36.0 41.0 30.0 41.0 48-49 36.03806800888481 39.0 36.0 41.0 29.5 41.0 50-51 35.13829725231672 38.5 35.0 40.0 27.5 40.5 52-53 35.23052434578199 38.5 35.5 40.0 28.0 40.5 54-55 35.600200102317686 39.0 36.0 40.5 28.0 41.0 56-57 35.42649898653681 39.0 35.0 41.0 27.0 41.0 58-59 35.23142383484112 39.0 35.0 40.0 26.0 41.0 60-61 34.97296222664016 39.0 35.0 40.0 26.0 41.0 62-63 34.662140511977 38.0 34.5 40.0 25.0 41.0 64-65 34.35338781642393 38.0 34.0 40.0 23.5 41.0 66-67 33.99533483140247 38.0 34.0 40.0 22.5 41.0 68-69 33.563514677407866 37.0 33.5 40.0 22.0 41.0 70-71 33.17109395742807 36.5 33.0 39.0 20.5 41.0 72-73 32.73166926778093 36.0 32.5 39.0 18.5 40.0 74-75 32.28544627997488 35.5 32.0 38.5 17.5 40.0 76-77 31.792733501272494 35.0 31.5 37.5 13.5 39.5 78-79 31.259772310760845 35.0 31.0 37.0 8.0 39.0 80-81 30.71834465519586 35.0 31.0 36.5 7.0 39.0 82-83 30.187111208967693 34.0 30.0 36.0 2.0 37.5 84-85 29.69436410850856 34.0 29.5 35.5 2.0 37.0 86-87 29.212689336294936 34.0 29.0 35.0 2.0 37.0 88-89 28.740846776021396 34.0 29.0 35.0 2.0 36.0 90-91 28.266461167846344 34.0 28.5 35.0 2.0 36.0 92-93 27.732189274774804 34.0 27.0 35.0 2.0 35.5 94-95 27.144255638805603 33.0 26.0 35.0 2.0 35.0 96-97 26.470454148075717 33.0 25.0 35.0 2.0 35.0 98-99 25.59222320798337 32.0 22.0 34.5 2.0 35.0 100-101 23.189469696479108 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.003018837936711094 1101 2 0.10226214846889548 1101 3 0.04475449632818851 1101 4 -0.12227720905715955 1101 5 -0.08042381917658759 1101 6 -0.07136261709362657 1101 7 0.0014346839651295795 1101 8 0.004138867924552869 1101 9 -0.11981217884510187 1101 10-11 0.07941323780033116 1101 12-13 0.13170359610292337 1101 14-15 0.1895603644645547 1101 16-17 0.28533558285854355 1101 18-19 0.20011084145748725 1101 20-21 0.34644214756519887 1101 22-23 0.2692980339191351 1101 24-25 0.181882677168403 1101 26-27 0.14428936856502617 1101 28-29 0.007781221620049905 1101 30-31 0.1842151307747102 1101 32-33 0.08156738194746538 1101 34-35 0.26820352285757565 1101 36-37 0.30235114745774894 1101 38-39 0.24644420747607398 1101 40-41 0.2718223992981166 1101 42-43 0.225908186805313 1101 44-45 0.17539472878195994 1101 46-47 0.2809344546580377 1101 48-49 0.4221675561638065 1101 50-51 0.4561367438083508 1101 52-53 0.334661133388515 1101 54-55 0.4914959601497486 1101 56-57 0.3901294611259445 1101 58-59 0.39948285906042713 1101 60-61 0.47962842855182686 1101 62-63 0.7295374791328086 1101 64-65 0.5366186627531917 1101 66-67 0.6972671422362353 1101 68-69 0.6967482771708475 1101 70-71 0.7385278930780146 1101 72-73 0.7816065489369279 1101 74-75 0.893343784911174 1101 76-77 0.9774132232608004 1101 78-79 0.8301672863366996 1101 80-81 0.9205559691346927 1101 82-83 0.7565104701582541 1101 84-85 1.0428487481685096 1101 86-87 1.085744337057836 1101 88-89 0.7632250867226418 1101 90-91 1.075720138231457 1101 92-93 1.031978814425674 1101 94-95 1.2336705262936825 1101 96-97 1.2350727760724673 1101 98-99 1.359144553690804 1101 100-101 1.6840286783397254 1102 1 0.12158392510396254 1102 2 0.1264553751632178 1102 3 0.1733772752361773 1102 4 0.19078540479527106 1102 5 0.1568568417548022 1102 6 0.009975112993558355 1102 7 -0.016265352822223633 1102 8 0.21790443052732655 1102 9 0.18216990615584905 1102 10-11 0.18355912847540168 1102 12-13 0.1788346255726978 1102 14-15 0.21293806423540929 1102 16-17 0.18419029939645526 1102 18-19 0.1868607450568902 1102 20-21 0.3698730338745406 1102 22-23 0.48551350145097416 1102 24-25 0.28780588467125057 1102 26-27 0.4431755538048776 1102 28-29 0.2810370355735401 1102 30-31 0.3805654261816116 1102 32-33 0.30940078398007387 1102 34-35 0.47381270817277965 1102 36-37 0.648447033922217 1102 38-39 0.45470053113389497 1102 40-41 0.45084828784572295 1102 42-43 0.4472805324646245 1102 44-45 0.5320259820784159 1102 46-47 0.5854805740352234 1102 48-49 0.7569601951468741 1102 50-51 0.6155356916013304 1102 52-53 0.733351858099418 1102 54-55 0.7142223464347097 1102 56-57 0.7352690979505283 1102 58-59 0.4735240608989031 1102 60-61 0.8214749322017596 1102 62-63 0.8605237348448256 1102 64-65 0.7160312617577134 1102 66-67 0.6758462638605494 1102 68-69 0.7953804966894253 1102 70-71 0.8466760707742438 1102 72-73 0.8763927168911536 1102 74-75 1.1255340508881915 1102 76-77 0.9718031515032983 1102 78-79 0.9750030950601847 1102 80-81 1.086470267892345 1102 82-83 1.0444457687341817 1102 84-85 1.206740850410764 1102 86-87 1.076700409409252 1102 88-89 0.9800023370672442 1102 90-91 1.2770744004676864 1102 92-93 1.4306761874538942 1102 94-95 1.5762959911218637 1102 96-97 1.64583019670264 1102 98-99 1.673010505884971 1102 100-101 2.106077157869553 1103 1 0.162474017756999 1103 2 0.22816424393884915 1103 3 0.3201339319328227 1103 4 0.30795349551925 1103 5 0.25687535287608654 1103 6 0.18545080009715065 1103 7 0.1699248635630397 1103 8 0.2470984173357138 1103 9 0.278525163801433 1103 10-11 0.23996811708619248 1103 12-13 0.24077502950484586 1103 14-15 0.3669676430586861 1103 16-17 0.49634138911694237 1103 18-19 0.24216515557510832 1103 20-21 0.13967993365142917 1103 22-23 0.41236047293410394 1103 24-25 0.43756938074007223 1103 26-27 0.3758827565795926 1103 28-29 0.27448629074447695 1103 30-31 0.30824661595956826 1103 32-33 0.382779112328393 1103 34-35 0.38608095380561736 1103 36-37 0.38630519976238276 1103 38-39 0.6235901561375456 1103 40-41 0.37562859006413873 1103 42-43 0.2228794646652119 1103 44-45 0.12298865781228585 1103 46-47 0.5794157461399365 1103 48-49 0.6287329272909474 1103 50-51 0.5856097847999493 1103 52-53 0.3582617381668385 1103 54-55 0.627315067666963 1103 56-57 0.398507959613994 1103 58-59 0.5349517554848902 1103 60-61 0.5387102475240937 1103 62-63 0.5696178103117475 1103 64-65 0.6053530240164591 1103 66-67 0.4918067474879493 1103 68-69 0.7454698859198388 1103 70-71 0.7247754657528489 1103 72-73 0.6362182252162896 1103 74-75 0.7110383815163033 1103 76-77 0.7885576648069481 1103 78-79 1.0575955775963983 1103 80-81 0.9120772378166428 1103 82-83 0.8196245471950334 1103 84-85 0.7616859990355849 1103 86-87 0.9766173529308482 1103 88-89 0.8937161551321502 1103 90-91 0.9801523615163923 1103 92-93 1.0605536243455838 1103 94-95 1.1634858080566168 1103 96-97 1.3018326713472739 1103 98-99 1.80238741179976 1103 100-101 2.4173489693289127 1104 1 0.06832865994482518 1104 2 0.1346454775264334 1104 3 0.28999346153356953 1104 4 0.07361258422750439 1104 5 0.06926534336329127 1104 6 0.13106266659900712 1104 7 0.017980721338183514 1104 8 0.0026280864384915503 1104 9 0.2083526233150863 1104 10-11 0.2664793416166802 1104 12-13 0.0883573835517808 1104 14-15 0.35546257051649377 1104 16-17 0.39826844248543836 1104 18-19 0.32858492624265523 1104 20-21 0.3781193223260715 1104 22-23 0.38684681039344326 1104 24-25 0.2527242057786694 1104 26-27 0.3550770739652265 1104 28-29 0.24758344658914666 1104 30-31 0.3862964071907058 1104 32-33 0.398920249193047 1104 34-35 0.3757300911031791 1104 36-37 0.34761887711337636 1104 38-39 0.4520251586723987 1104 40-41 0.3965104604964296 1104 42-43 0.41582546957540956 1104 44-45 0.43684544180344886 1104 46-47 0.6892458259448944 1104 48-49 0.59290400383194 1104 50-51 0.5181351475109324 1104 52-53 0.582251786756153 1104 54-55 0.6935785322617036 1104 56-57 0.7012085685469103 1104 58-59 0.6179210012397363 1104 60-61 0.6801205338763694 1104 62-63 0.8056939525593876 1104 64-65 0.779128892272773 1104 66-67 0.5597050460074868 1104 68-69 0.6115167492602609 1104 70-71 0.6689490201780117 1104 72-73 0.822755908992697 1104 74-75 0.8917274042046088 1104 76-77 0.8476181764473161 1104 78-79 0.8118001482958448 1104 80-81 0.9058718468520901 1104 82-83 1.1702287806743783 1104 84-85 1.111155247638571 1104 86-87 1.0190639360140246 1104 88-89 1.2991236389119791 1104 90-91 1.3492467375737611 1104 92-93 1.4034742807855203 1104 94-95 1.2353601138218302 1104 96-97 1.5055603205472288 1104 98-99 1.7833554100680864 1104 100-101 1.9835326859273188 1105 1 0.18054646353316173 1105 2 0.14991097620926297 1105 3 0.16515320357523677 1105 4 0.01727180585444188 1105 5 0.00764719352918064 1105 6 0.04299663930730446 1105 7 0.07700987328010456 1105 8 -0.03880111013531007 1105 9 0.015481869228040068 1105 10-11 0.09261723670037014 1105 12-13 0.07106514409474585 1105 14-15 0.27581732424788186 1105 16-17 0.3365622492886615 1105 18-19 0.24630709625874658 1105 20-21 0.35024618845308453 1105 22-23 0.22993959066336345 1105 24-25 0.158402741155534 1105 26-27 0.06510233630829987 1105 28-29 0.09804920469480294 1105 30-31 0.27459030116558125 1105 32-33 0.20479509511716287 1105 34-35 0.1759152436797109 1105 36-37 0.01773260124301146 1105 38-39 0.0065937374781697144 1105 40-41 0.14397158630080753 1105 42-43 0.12593790356467593 1105 44-45 0.2228957119483752 1105 46-47 0.2147592233552018 1105 48-49 0.2801347524097295 1105 50-51 0.18928098393158876 1105 52-53 0.17008942433480456 1105 54-55 0.35746820085253717 1105 56-57 0.18940901451705372 1105 58-59 0.17593373178694804 1105 60-61 0.2021714734995399 1105 62-63 0.17421034344402386 1105 64-65 0.44634328744974283 1105 66-67 0.5144891131226785 1105 68-69 0.5456533402561305 1105 70-71 0.6321285885388903 1105 72-73 0.4739708202258086 1105 74-75 0.3310620404634861 1105 76-77 0.5485087219849056 1105 78-79 0.5393695761534616 1105 80-81 0.7594940902667098 1105 82-83 0.6835669142596998 1105 84-85 0.5735738075281063 1105 86-87 0.6303298252258323 1105 88-89 0.5536805367171702 1105 90-91 0.5913014778807124 1105 92-93 0.7931019009472742 1105 94-95 0.889401129824801 1105 96-97 0.8542983366251704 1105 98-99 1.0472989507362769 1105 100-101 1.5606464675902423 1106 1 0.2798919325035385 1106 2 0.1711005918651196 1106 3 0.2179233904581679 1106 4 0.17681870289252544 1106 5 0.3033255256871499 1106 6 0.1823258000971535 1106 7 0.1913390340699479 1106 8 0.14354859014677146 1106 9 0.2817437697922145 1106 10-11 0.049317915473288565 1106 12-13 0.05767811130208145 1106 14-15 -0.0037588834390049897 1106 16-17 0.12935910225058223 1106 18-19 0.2989659908285631 1106 20-21 0.4913648414855274 1106 22-23 0.2190064982797324 1106 24-25 0.1627133899566644 1106 26-27 0.07912296395286944 1106 28-29 0.38746872162005275 1106 30-31 0.406417698908875 1106 32-33 0.6066738415910748 1106 34-35 0.4635111093355704 1106 36-37 0.38198132303426746 1106 38-39 0.4373214349393919 1106 40-41 0.7434173285434014 1106 42-43 0.5408158125914753 1106 44-45 0.38031152647587874 1106 46-47 0.10663708830583118 1106 48-49 0.37356299641538016 1106 50-51 0.3556486672884276 1106 52-53 0.39807668632352744 1106 54-55 0.40956953195267687 1106 56-57 0.2656486566186089 1106 58-59 0.40739199166000617 1106 60-61 0.3101856219480581 1106 62-63 0.5794752411873176 1106 64-65 0.7499850804680719 1106 66-67 0.44278601016075925 1106 68-69 0.5592695690995697 1106 70-71 0.8125058804993941 1106 72-73 0.7036109383499252 1106 74-75 0.8649698331292015 1106 76-77 1.115022958585751 1106 78-79 1.0650516547853321 1106 80-81 0.9683776410424478 1106 82-83 0.7573801635544797 1106 84-85 0.5594826580217607 1106 86-87 0.7708137815022802 1106 88-89 0.584002733473163 1106 90-91 0.9499536287974948 1106 92-93 1.0586707038386756 1106 94-95 0.9025843103607656 1106 96-97 1.106757786554649 1106 98-99 1.249964456730634 1106 100-101 1.6814834739372095 1107 1 -0.026964988984062188 1107 2 0.05814862905520357 1107 3 0.03676378302014882 1107 4 -0.04957592520664633 1107 5 -0.10270494951946318 1107 6 -0.13190457180367332 1107 7 -0.08962687502096145 1107 8 0.0990883628740491 1107 9 0.1606823028500699 1107 10-11 0.05982282249808435 1107 12-13 0.043480022459107204 1107 14-15 0.29229612689157136 1107 16-17 0.07200502167206224 1107 18-19 0.24276118710955075 1107 20-21 0.15115306462602973 1107 22-23 0.28188098175079546 1107 24-25 0.10088487755997022 1107 26-27 0.04603928626691811 1107 28-29 0.08959940343822836 1107 30-31 -0.011996557289471355 1107 32-33 0.11588742630181059 1107 34-35 0.2783677725587097 1107 36-37 0.0568844738607126 1107 38-39 0.13500996799724163 1107 40-41 0.19994496284091667 1107 42-43 0.23592170102122623 1107 44-45 0.4770961339138964 1107 46-47 0.3394302184711222 1107 48-49 0.4032892360847953 1107 50-51 0.7157855474537271 1107 52-53 0.4295655809516248 1107 54-55 0.646146236498133 1107 56-57 0.6218922000070393 1107 58-59 0.5524785102550496 1107 60-61 0.7536967273199551 1107 62-63 0.8826648073030228 1107 64-65 0.8306862684846053 1107 66-67 0.5245008861938203 1107 68-69 0.8394800546367591 1107 70-71 0.9295513350448559 1107 72-73 0.822212436283813 1107 74-75 0.8682497918068961 1107 76-77 0.8276843738750053 1107 78-79 0.9863907560250063 1107 80-81 0.7630703063317092 1107 82-83 0.5264465375214229 1107 84-85 0.77479774066639 1107 86-87 1.0929986988576523 1107 88-89 1.2142325888450642 1107 90-91 1.1679546618553474 1107 92-93 1.0010970984667722 1107 94-95 1.138663855815313 1107 96-97 1.302057373331504 1107 98-99 1.6985246323504697 1107 100-101 2.3226637425322494 1108 1 -0.022444952382222993 1108 2 -0.0861352385391001 1108 3 -0.043623731685947575 1108 4 -0.1483021231180217 1108 5 -0.1135983539262071 1108 6 -0.0518075478817579 1108 7 -0.05821610652758835 1108 8 -0.035501827252168994 1108 9 -0.02537123460145807 1108 10-11 0.06710542645746642 1108 12-13 0.18796287624057584 1108 14-15 0.2917993876506273 1108 16-17 0.29651227667940105 1108 18-19 0.34140750005526854 1108 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82-83 -0.96277543944759 2316 84-85 -0.7336138740693379 2316 86-87 -0.8114261253300192 2316 88-89 -0.8517924269823212 2316 90-91 -0.8740435244116149 2316 92-93 -0.7167288173828616 2316 94-95 -0.5413662589974102 2316 96-97 -0.11157813477040435 2316 98-99 0.1292006756747348 2316 100-101 -0.17196179003794398 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2504.0 3 341.0 4 306.0 5 355.0 6 411.0 7 513.0 8 654.0 9 804.0 10 1032.0 11 1448.0 12 2103.0 13 3043.0 14 3544.0 15 4651.0 16 6641.0 17 9076.0 18 7943.0 19 4756.0 20 4394.0 21 4114.0 22 4311.0 23 4946.0 24 5501.0 25 6371.0 26 7068.0 27 8526.0 28 10065.0 29 12460.0 30 15365.0 31 19529.0 32 25458.0 33 34315.0 34 47382.0 35 68891.0 36 104553.0 37 161377.0 38 154932.0 39 22419.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.78021869536188 20.257638620073315 15.294516508668057 22.667626175896746 2 14.178528754740505 20.60249883110811 42.77598836303185 22.442984051119538 3 18.07143803258422 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20.043299480068875 27.652433762954487 31.850744603135677 20.453522153840964 24-25 19.81937560013495 27.3597306205071 32.53899255183869 20.28190122751927 26-27 19.933622074066864 27.637484386912583 32.22709945333918 20.201794085681378 28-29 19.60855189510333 28.08354613565053 32.116331212340484 20.191570756905666 30-31 20.046085640643614 27.66858052073789 32.27714458509946 20.008189253519042 32-33 19.65583166551152 27.601412651564118 32.22069500795622 20.522060674968138 34-35 20.06630518293949 27.882374164209477 32.031152664147186 20.02016798870385 36-37 19.904416549717734 27.772014136384843 31.90276077341363 20.420808540483794 38-39 19.680098000158317 28.03625953950826 32.048076136987405 20.23556632334602 40-41 20.363650519199513 27.73067153696681 31.823864686971838 20.081813256861835 42-43 19.91031407592742 27.937275655417103 31.622042155369083 20.53036811328639 44-45 20.31211273015589 28.07398987048074 31.246115136014037 20.367782263349334 46-47 20.21851647827585 28.081294514508397 30.793187954147744 20.907001053068008 48-49 20.235108761811567 28.08917676892868 30.935438531829103 20.74027593743065 50-51 20.4281285631683 28.552265858674485 30.374336686916227 20.64526889124099 52-53 20.432053252471547 28.47833762565423 30.338487579961786 20.751121541912436 54-55 20.54407502120224 28.247219051710097 30.319230736786647 20.889475190301024 56-57 20.63192009984924 28.58132601243964 29.84792971111705 20.938824176594075 58-59 20.670325100667483 28.56387544024792 29.95015767508876 20.815641783995833 60-61 20.630659328610697 28.31466272558651 30.232176419564144 20.822501526238653 62-63 20.507684761281883 28.23617729193269 30.408660084919077 20.847477861866352 64-65 20.472151814149502 28.319961386028776 30.186822333563207 21.02106446625851 66-67 20.28699572516766 28.300960134220265 30.287144945297843 21.124899195314228 68-69 20.57008386524984 28.235845224949102 30.143023794477585 21.051047115323477 70-71 20.434912645722918 28.002351159717314 30.301479141567196 21.261257052992573 72-73 20.389997301056738 27.87443945024602 30.36713413748002 21.368429111217225 74-75 20.410134771728885 27.97261096949616 30.13954677304981 21.477707485725148 76-77 20.262821332236296 27.958897505260776 30.23295350208906 21.545327660413868 78-79 20.194514579833324 27.886717449663124 30.17214696051502 21.746621009988534 80-81 20.14342275843543 28.109536990535812 30.034947293563754 21.712092957465003 82-83 20.290282937945193 28.04904794100128 30.23243138725631 21.428237733797218 84-85 20.42303277949314 27.889591831305932 30.115376704974178 21.571998684226745 86-87 20.03748866191819 28.085807381723853 29.943760518950008 21.93294343740795 88-89 20.378626306852503 27.64703935821563 29.96178958904181 22.01254474589006 90-91 20.247740572498603 27.535914986221854 30.257155259280218 21.959189181999324 92-93 20.219755978082794 27.376845492796452 30.583193566282024 21.820204962838726 94-95 19.97901705885864 27.58354895889835 30.318014185869668 22.11941979637334 96-97 19.767759447625856 27.537158942506874 30.48755724433136 22.207524365535914 98-99 19.911518204360636 27.41754869483188 30.51155687216398 22.1593762286435 100-101 19.805489905028576 27.139354785972074 30.801259365607226 22.253895943392127 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 970.0 1 548.5 2 110.5 3 84.0 4 81.0 5 82.5 6 88.0 7 158.0 8 294.5 9 404.5 10 402.0 11 412.0 12 481.5 13 522.5 14 594.5 15 678.0 16 801.0 17 963.5 18 1084.0 19 1275.5 20 1535.0 21 1875.0 22 2358.0 23 2934.0 24 3764.0 25 4886.5 26 6296.0 27 7998.0 28 9760.0 29 11536.5 30 13740.5 31 16260.5 32 18953.0 33 21689.0 34 24352.0 35 26767.0 36 28665.0 37 30033.0 38 30547.5 39 30731.0 40 30920.0 41 30756.0 42 30473.0 43 30234.5 44 29765.0 45 29298.5 46 28710.0 47 28639.0 48 29690.0 49 28568.0 50 25715.0 51 22976.5 52 20148.0 53 17815.5 54 15674.5 55 13593.5 56 11626.0 57 9866.0 58 7979.5 59 6298.0 60 4922.0 61 3821.0 62 2851.5 63 1972.0 64 1404.0 65 1052.0 66 791.0 67 586.0 68 424.5 69 292.0 70 221.0 71 176.0 72 140.0 73 113.0 74 99.5 75 80.5 76 56.0 77 39.5 78 21.0 79 16.0 80 13.5 81 7.0 82 3.0 83 2.5 84 3.0 85 3.0 86 3.5 87 2.5 88 1.0 89 1.5 90 2.0 91 1.0 92 0.0 93 1.5 94 2.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29361291534182526 2 0.2778119556277967 3 0.26408325292544405 4 0.25035455022309144 5 0.2456919719468207 6 0.20580102447206014 7 0.19777102855181614 8 0.21059311881156062 9 0.21111118306447957 10-11 0.21208255353870262 12-13 0.21182352141224317 14-15 0.2068371529778981 16-17 0.20599529856690477 18-19 0.20567150840883042 20-21 0.19369127256007926 22-23 0.1846899061656122 24-25 0.1871507113669773 26-27 0.19654062595113359 28-29 0.21143497322255392 30-31 0.20502392809268172 32-33 0.21227682763354724 34-35 0.20424683171330324 36-37 0.19032385491610598 38-39 0.19349699846523466 40-41 0.18747450152505166 42-43 0.19777102855181614 44-45 0.1924608699593967 46-47 0.19420933681299823 48-49 0.20308118714423556 50-51 0.21182352141224317 52-53 0.22496940183006198 54-55 0.20068513997448537 56-57 0.1739400729175436 58-59 0.17801982890928048 60-61 0.18384805175461885 62-63 0.19284941814908593 64-65 0.18125773049002403 66-67 0.18540224451337575 68-69 0.18961151656834238 70-71 0.18410708388107835 72-73 0.18559651860822038 74-75 0.18540224451337575 76-77 0.1999080435951069 78-79 0.20353449336553966 80-81 0.19634635185628896 82-83 0.19582828760337 84-85 0.18851063003088958 86-87 0.19064764507418033 88-89 0.1947274010659172 90-91 0.19433885287622799 92-93 0.1913599834219439 94-95 0.19155425751678853 96-97 0.18073966623710505 98-99 0.1862440989243691 100-101 0.18125773049002403 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 772105.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.37306942831113 #Duplication Level Percentage of deduplicated Percentage of total 1 85.89543197512583 68.17784085736439 2 10.340900195389754 16.415779783198143 3 2.3094534811024356 5.499252344909953 4 0.7165919124312358 2.275123984686829 5 0.2996772292634005 1.1893150762203901 6 0.13545967841594736 0.6451110275787413 7 0.07050100346241071 0.391711672981127 8 0.045755919953248875 0.2905430248964376 9 0.03326166941777507 0.2376072716398735 >10 0.13481136264458451 1.874964512303364 >50 0.010795590881689484 0.6144750344467022 >100 0.006378651019623968 0.9082119354413625 >500 3.271102973679395E-4 0.18719862555808395 >1k 6.54220594735879E-4 1.2928648487745893 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3948 0.5113294176310217 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2986 0.3867349648040098 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1961 0.2539809999935242 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1064 0.13780509127644555 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.2951606322974206E-4 0.0 3 0.0 0.0 0.0 1.2951606322974206E-4 0.0 4 0.0 0.0 0.0 1.2951606322974206E-4 0.0 5 0.0 0.0 0.0 1.2951606322974206E-4 0.0 6 0.0 0.0 0.0 1.2951606322974206E-4 0.0 7 0.0 0.0 0.0 1.2951606322974206E-4 0.0 8 0.0 0.0 0.0 1.2951606322974206E-4 0.0 9 0.0 0.0 0.0 1.2951606322974206E-4 0.0 10-11 0.0 0.0 0.0 1.942740948446131E-4 0.0 12-13 0.0 0.0 0.0 2.5903212645948413E-4 0.0 14-15 0.0 0.0 0.0 2.5903212645948413E-4 0.0 16-17 0.0 0.0 0.0 3.885481896892262E-4 0.0 18-19 0.0 0.0 0.0 3.885481896892262E-4 0.0 20-21 0.0 0.0 0.0 4.533062213040972E-4 0.0 22-23 0.0 0.0 0.0 6.475803161487103E-4 0.0 24-25 0.0 0.0 0.0 9.066124426081945E-4 0.0 26-27 0.0 0.0 0.0 0.001813224885216389 0.0 28-29 0.0 0.0 0.0 0.004079755991736875 0.0 30-31 0.0 0.0 0.0 0.0093899145841563 0.0 32-33 0.0 0.0 0.0 0.01819700688377876 0.0 34-35 0.0 0.0 0.0 0.024867084140110476 0.0 36-37 0.0 0.0 0.0 0.029918210606070417 0.0 38-39 0.0 0.0 0.0 0.03594070754625342 0.0 40-41 0.0 0.0 0.0 0.042805058897429756 0.0 42-43 0.0 0.0 0.0 0.051417877102207604 0.0 44-45 0.0 0.0 0.0 0.06022496940183006 0.0 46-47 0.0 0.0 0.0 0.07356512391449349 0.0 48-49 0.0 0.0 0.0 0.09156785670342764 0.0 50-51 0.0 0.0 0.0 0.10749833248068591 0.0 52-53 1.2951606322974206E-4 0.0 0.0 0.15049766547296028 0.0 54-55 1.2951606322974206E-4 0.0 0.0 0.21182352141224314 0.0 56-57 1.2951606322974206E-4 0.0 0.0 0.29050452982431146 0.0 58-59 1.2951606322974206E-4 0.0 0.0 0.3886129477208411 0.0 60-61 1.2951606322974206E-4 0.0 0.0 0.5268713452185907 0.0 62-63 1.2951606322974206E-4 0.0 0.0 0.6172087993213358 0.0 64-65 1.2951606322974206E-4 0.0 0.0 0.7147991529649464 0.0 66-67 1.2951606322974206E-4 0.0 0.0 0.8952150290439772 0.0 68-69 1.2951606322974206E-4 0.0 0.0 1.1162989489771469 0.0 70-71 1.2951606322974206E-4 0.0 0.0 1.3039677245970434 0.0 72-73 1.2951606322974206E-4 0.0 0.0 1.4973352069990482 0.0 74-75 1.2951606322974206E-4 0.0 0.0 1.837314872977121 0.0 76-77 1.2951606322974206E-4 0.0 0.0 2.106125462210451 0.0 78-79 1.2951606322974206E-4 0.0 0.0 2.3100485037656795 0.0 80-81 1.2951606322974206E-4 0.0 0.0 2.5010846970295493 0.0 82-83 1.2951606322974206E-4 0.0 0.0 2.8318039644866957 0.0 84-85 1.2951606322974206E-4 0.0 0.0 3.2068824836000287 0.0 86-87 1.2951606322974206E-4 0.0 0.0 3.683631112348709 0.0 88-89 1.2951606322974206E-4 0.0 0.0 4.387227125844284 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATTGCG 15 6.616337E-4 95.00272 7 GGGCGTA 50 0.0016152575 38.001087 7 GGTATCA 3000 0.0 30.585022 1 CCGTAGG 65 0.0058420715 29.231607 9 GTATCAA 4335 0.0 28.73326 1 GTATAGC 505 0.0 27.30105 1 GTACATG 2935 0.0 26.564917 1 TACATGG 2935 0.0 25.583057 2 ATCAACG 4965 0.0 25.171654 3 TCAACGC 4965 0.0 25.171654 4 CAACGCA 5020 0.0 24.89587 5 TATCAAC 5100 0.0 24.786484 2 ACATGGG 2970 0.0 24.639935 3 AACGCAG 5120 0.0 24.400114 6 ATAGCCC 590 0.0 23.357225 3 GAGTACT 3210 0.0 22.935307 12-13 ACGCAGA 5600 0.0 22.139029 7 CGCAGAG 5605 0.0 22.119278 8 GTCCTAC 430 0.0 22.112318 1 CATGGGT 980 0.0 21.820349 4 >>END_MODULE