FastQCFastQC Report
Thu 26 May 2016
SRR937709_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937709_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772105
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44560.5771235777517306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35770.4632789581727874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23860.3090253268661646No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9580.1240763885740929No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9110.11798913360229503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44300.033.1496351
GGTATCA28750.031.9038771
ATGGGCG953.489082E-530.0003687
ATCAACG52400.027.646453
TATCAAC53150.027.5244242
TCAACGC53200.027.1414344
CAACGCA53950.026.852165
GTATAGC4450.026.6995541
AACGCAG54750.026.4597996
TACATGG31400.023.7487552
GTACATG31500.023.6871661
CGCAGAG61050.023.575238
ACGCAGA61650.023.4228347
TAGGACC2853.6379788E-1223.332114
GAGTACT36500.023.03452712-13
TAGGACG2303.623427E-922.71624
ACATGGG32350.022.4640453
AGTACTT37800.021.92817912-13
GCAGAGT65600.021.7228289
GTGTAGC5400.021.1223141
Produced by FastQC (version 0.11.5)