FastQCFastQC Report
Thu 26 May 2016
SRR937708_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937708_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601911
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30650.5092114947226417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23710.3939120567658673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15870.2636602421288197No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9170.15234810462011825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG608.264335E-539.6009372
GTATCAA31900.036.661661
GGTATCA18800.034.391311
TCACGCA702.0468139E-433.943664
ATCAACG35300.032.8474663
TCAACGC35800.032.38874
TATCAAC36100.032.119542
CAACGCA36100.032.119545
AACGCAG36400.031.9773776
TAGGACG1201.4979378E-731.680754
ATCACGC650.005829197429.2437693
CGCAGAG41650.027.9489468
ACGCAGA42000.027.7160367
GTACATG23100.027.1662481
TACATGG23600.026.1768912
GCAGAGT43500.026.1006099
ACATGGG24600.025.112793
GAGTACT26150.024.25020212-13
AGAGTAC36400.022.0541910-11
GTACTTT28850.021.97702414-15
Produced by FastQC (version 0.11.5)