##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937708_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 601911 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07830559667459 33.0 31.0 34.0 31.0 34.0 2 32.20844609917413 33.0 31.0 34.0 31.0 34.0 3 32.25870768269728 34.0 31.0 34.0 31.0 34.0 4 35.83249683092683 37.0 35.0 37.0 35.0 37.0 5 35.84269269044759 37.0 35.0 37.0 35.0 37.0 6 35.904464281264175 37.0 36.0 37.0 35.0 37.0 7 35.83655224775756 37.0 36.0 37.0 35.0 37.0 8 35.847615345125774 37.0 36.0 37.0 35.0 37.0 9 37.4658180362213 39.0 37.0 39.0 35.0 39.0 10-11 37.425610264640454 39.0 37.0 39.0 35.0 39.0 12-13 37.359626257038 39.0 37.0 39.0 35.0 39.0 14-15 38.575107449440196 40.0 38.0 41.0 34.5 41.0 16-17 38.496942238968884 40.0 38.0 41.0 34.0 41.0 18-19 38.45471008172305 40.0 38.0 41.0 34.0 41.0 20-21 38.487863654261176 40.0 38.0 41.0 34.0 41.0 22-23 38.488509098521206 40.0 38.0 41.0 34.0 41.0 24-25 38.44112667819661 40.0 38.0 41.0 34.0 41.0 26-27 38.351347624482685 40.0 38.0 41.0 34.0 41.0 28-29 38.220836635316516 40.0 38.0 41.0 34.0 41.0 30-31 38.00102589917778 40.0 38.0 41.0 34.0 41.0 32-33 37.85619967071544 40.0 37.5 41.0 34.0 41.0 34-35 37.84366791768218 40.0 38.0 41.0 33.5 41.0 36-37 37.719927863089396 40.0 37.5 41.0 33.0 41.0 38-39 37.55925377672114 40.0 37.0 41.0 33.0 41.0 40-41 37.35220904751699 40.0 37.0 41.0 33.0 41.0 42-43 37.08747887976794 40.0 37.0 41.0 32.0 41.0 44-45 36.79625310054144 40.0 37.0 41.0 31.0 41.0 46-47 36.39028776679609 40.0 36.5 41.0 30.0 41.0 48-49 36.02155468167221 39.0 36.0 41.0 29.5 41.0 50-51 35.161732382362175 38.5 35.0 40.0 27.5 40.5 52-53 35.26672963278624 38.5 35.5 40.0 28.0 40.5 54-55 35.5509419166621 39.0 36.0 40.0 28.0 41.0 56-57 35.33509688309401 39.0 35.0 40.0 26.5 41.0 58-59 35.1165080884051 39.0 35.0 40.0 26.0 41.0 60-61 34.82681658916351 38.5 35.0 40.0 25.5 41.0 62-63 34.51859411108951 38.0 34.0 40.0 24.5 41.0 64-65 34.135068971990876 38.0 34.0 40.0 22.5 41.0 66-67 33.65717024609951 37.5 34.0 40.0 21.0 41.0 68-69 33.27343162028937 37.0 33.0 39.5 20.0 41.0 70-71 32.86145958455652 36.0 32.5 39.0 18.5 40.0 72-73 32.40695468266903 36.0 32.0 39.0 16.0 40.0 74-75 31.8845036890836 35.5 31.5 38.5 10.5 40.0 76-77 31.344733689864448 35.0 31.0 37.5 7.5 39.0 78-79 30.82709071606932 35.0 31.0 37.0 4.5 39.0 80-81 30.28782577490692 34.5 30.0 36.5 2.0 39.0 82-83 29.74239048630113 34.0 29.5 36.0 2.0 37.5 84-85 29.188975612673634 34.0 29.0 35.5 2.0 37.0 86-87 28.614302612844757 34.0 28.5 35.0 2.0 36.5 88-89 28.08598779553788 34.0 27.5 35.0 2.0 36.0 90-91 27.552412233702324 33.0 26.5 35.0 2.0 36.0 92-93 26.93085356472967 33.0 25.5 35.0 2.0 35.0 94-95 26.225190268993256 32.0 25.0 34.5 2.0 35.0 96-97 25.400986192310825 32.0 21.5 34.0 2.0 35.0 98-99 24.257776481905132 31.5 9.5 34.0 2.0 35.0 100-101 20.963189740675947 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.12238383744637105 1101 2 -0.17446004739050025 1101 3 -0.13636198679389366 1101 4 -0.05584336799535805 1101 5 -0.240561238313866 1101 6 -0.09616985457308402 1101 7 -0.032934551533614354 1101 8 -0.07030776431301433 1101 9 -0.0011243047630884462 1101 10-11 0.10735427390380892 1101 12-13 0.01373341339412093 1101 14-15 0.09431942907280444 1101 16-17 0.09218590439235186 1101 18-19 0.10547001357079466 1101 20-21 0.1790872650073254 1101 22-23 -0.18397388244681423 1101 24-25 -0.05143640495998625 1101 26-27 0.1452094139877218 1101 28-29 0.2117248775599947 1101 30-31 0.30155826838950617 1101 32-33 0.20457643825415772 1101 34-35 0.2264228217364348 1101 36-37 0.37540828892006317 1101 38-39 0.275543932209942 1101 40-41 0.39716085612514007 1101 42-43 0.5293969937793008 1101 44-45 0.7751738039683502 1101 46-47 0.8359847388918951 1101 48-49 0.8955202698838463 1101 50-51 1.0845136788167835 1101 52-53 0.997032541860996 1101 54-55 1.0178286789043653 1101 56-57 1.1735397824605442 1101 58-59 1.0932594160314721 1101 60-61 1.2591292645965595 1101 62-63 1.1408730869304478 1101 64-65 1.1428870542658487 1101 66-67 1.1431297390514672 1101 68-69 1.41093977879936 1101 70-71 1.3017108248726927 1101 72-73 1.3995476156563633 1101 74-75 1.267066960115745 1101 76-77 1.1615198695664333 1101 78-79 1.3677532309061853 1101 80-81 1.3013196065668673 1101 82-83 1.5707154483441386 1101 84-85 1.9407668544939334 1101 86-87 2.1857087575912715 1101 88-89 1.9952080036049828 1101 90-91 2.1589254745005277 1101 92-93 2.250476091770622 1101 94-95 2.4826313627564893 1101 96-97 2.601495691894474 1101 98-99 2.8378976253243557 1101 100-101 3.5629062591451692 1102 1 -0.05689983246091046 1102 2 -0.07382024680886445 1102 3 0.008199708262196737 1102 4 -0.02491690351675402 1102 5 0.05972958013233409 1102 6 0.052920299145235106 1102 7 -0.05505337164163393 1102 8 0.02029464532554215 1102 9 0.09413120001464392 1102 10-11 0.18715485554069744 1102 12-13 -3.048084588073152E-4 1102 14-15 0.11699080422859964 1102 16-17 0.18029558449207173 1102 18-19 0.06036407256539178 1102 20-21 -0.010139988835632607 1102 22-23 0.09028037596615235 1102 24-25 0.16501145544715712 1102 26-27 0.06849150788053038 1102 28-29 0.06819351071330004 1102 30-31 0.211554113840279 1102 32-33 -0.042340886216138074 1102 34-35 0.12065215285400654 1102 36-37 0.07903105584984615 1102 38-39 0.16606740541310216 1102 40-41 0.3192962944300888 1102 42-43 0.307390346500533 1102 44-45 0.30821493400574695 1102 46-47 0.48368311861769087 1102 48-49 0.19643011616552286 1102 50-51 0.19018463851765688 1102 52-53 0.28078825436618615 1102 54-55 0.2666030868810978 1102 56-57 0.38690081278875255 1102 58-59 0.538577239047676 1102 60-61 0.5301928291997982 1102 62-63 0.45537246374806983 1102 64-65 0.5975152221096423 1102 66-67 0.7093532538001099 1102 68-69 0.5151441826215404 1102 70-71 0.408275012658315 1102 72-73 0.4976240593622201 1102 74-75 0.637432560448115 1102 76-77 0.612384015806569 1102 78-79 0.7582019222231793 1102 80-81 0.9505053149175104 1102 82-83 1.1017291583566013 1102 84-85 1.3238453754744093 1102 86-87 1.2377237139685064 1102 88-89 1.0753783401234713 1102 90-91 1.1559175808569897 1102 92-93 1.3702849825059786 1102 94-95 1.4604834608038502 1102 96-97 1.8415497010344843 1102 98-99 2.2381801346720955 1102 100-101 3.1228273227097745 1103 1 0.06440381951057361 1103 2 0.10621370246122552 1103 3 0.12446528855221572 1103 4 0.061940849041562274 1103 5 0.019174752061140055 1103 6 0.06824984955990487 1103 7 -0.012176413496234773 1103 8 -0.015204651439582051 1103 9 -0.02315673829468068 1103 10-11 0.01682845755826179 1103 12-13 -0.07750156872846503 1103 14-15 0.08493590340896873 1103 16-17 0.28055456892390396 1103 18-19 0.2505304239888346 1103 20-21 0.20677353752354577 1103 22-23 0.05143950104496042 1103 24-25 0.11942340559023279 1103 26-27 -0.0031307822402268926 1103 28-29 -0.0894494399632606 1103 30-31 0.015360312007018706 1103 32-33 0.2082561359478845 1103 34-35 0.06391710265759087 1103 36-37 -0.0426741674878528 1103 38-39 -0.03629837372264433 1103 40-41 0.1504491146444522 1103 42-43 -0.24423000366194003 1103 44-45 -0.07173365709954993 1103 46-47 0.0017965091797975674 1103 48-49 0.08433544620092448 1103 50-51 -0.23073296175393665 1103 52-53 -0.04161826389367462 1103 54-55 -0.018022743663543395 1103 56-57 -0.09822067709921356 1103 58-59 -0.008236864449102654 1103 60-61 0.14008176658764881 1103 62-63 0.4917127324322905 1103 64-65 0.24244562610595466 1103 66-67 0.3818737446095639 1103 68-69 0.33553605419631083 1103 70-71 0.4791513888162271 1103 72-73 0.4751962015611575 1103 74-75 0.41795828914494493 1103 76-77 0.4401322092204154 1103 78-79 0.535852632732901 1103 80-81 0.38324308105553584 1103 82-83 0.3248874104541848 1103 84-85 0.5689377659880144 1103 86-87 0.7377091642813234 1103 88-89 0.5912792082548073 1103 90-91 0.5442914108196426 1103 92-93 0.5284080728595342 1103 94-95 0.9199053532331654 1103 96-97 0.9107470432916251 1103 98-99 0.7896540179836045 1103 100-101 1.5510944549664316 1104 1 0.06605753611705012 1104 2 0.07635763255116501 1104 3 0.057047964249477445 1104 4 0.11458821424136545 1104 5 0.08989950957683845 1104 6 -0.018314918980571804 1104 7 0.08358918028125117 1104 8 0.1327442130488805 1104 9 0.07229675700560279 1104 10-11 -0.001622844946233215 1104 12-13 0.1309741342024182 1104 14-15 0.18872587329348534 1104 16-17 0.28813916990397104 1104 18-19 0.18463913964281176 1104 20-21 0.2278685573235606 1104 22-23 -0.01095384842021474 1104 24-25 0.019035106445862482 1104 26-27 0.03914538843305593 1104 28-29 -0.055067942133767644 1104 30-31 0.11909758994084285 1104 32-33 0.03319523621256337 1104 34-35 0.39041365538804484 1104 36-37 0.344520472524799 1104 38-39 0.3676067078080081 1104 40-41 0.357165717067474 1104 42-43 0.33633598895308126 1104 44-45 0.41737025548044926 1104 46-47 0.6104057659395679 1104 48-49 0.48783824495415473 1104 50-51 0.5004002799764677 1104 52-53 0.4205924876962044 1104 54-55 0.49005533770093024 1104 56-57 0.44561193273310806 1104 58-59 0.7404782625992965 1104 60-61 0.9171897486075267 1104 62-63 0.805874302166032 1104 64-65 1.0412586385858376 1104 66-67 1.2262865738835913 1104 68-69 0.950483544640818 1104 70-71 0.8315136094982236 1104 72-73 0.849792398823606 1104 74-75 0.8658256837711811 1104 76-77 1.0793352231999904 1104 78-79 1.375091517771228 1104 80-81 1.3979603482077785 1104 82-83 1.2205158024985074 1104 84-85 1.451478288124715 1104 86-87 1.3341810328594903 1104 88-89 1.1589488459368447 1104 90-91 1.396155084583512 1104 92-93 1.3263050560426954 1104 94-95 1.3957055615690308 1104 96-97 1.6200262984706377 1104 98-99 2.094503298738477 1104 100-101 2.603934348447627 1105 1 0.21039692062240078 1105 2 0.20299576124550356 1105 3 0.04595707045700692 1105 4 0.07907000813690246 1105 5 -0.09025783491926376 1105 6 0.09686945595369423 1105 7 0.008024906737425397 1105 8 0.07992968182188775 1105 9 0.22586540590440052 1105 10-11 0.29819940625804264 1105 12-13 0.34732544575714996 1105 14-15 0.40368096279895127 1105 16-17 0.21904589911578398 1105 18-19 0.38425156312668207 1105 20-21 0.13837624574779994 1105 22-23 0.09581901175774021 1105 24-25 0.2965782815231677 1105 26-27 0.19962415323669092 1105 28-29 0.2467789625129484 1105 30-31 0.16509929380504218 1105 32-33 0.3003837551128399 1105 34-35 0.4894691677042502 1105 36-37 0.522561133876529 1105 38-39 0.3390827590501502 1105 40-41 0.4352489750240949 1105 42-43 0.24179256145145445 1105 44-45 0.3226322508947419 1105 46-47 0.1919098993878947 1105 48-49 0.40266344110888497 1105 50-51 0.33933515450053875 1105 52-53 0.08404775600222791 1105 54-55 0.3487450450990721 1105 56-57 0.4849086154567672 1105 58-59 0.5306373951010386 1105 60-61 0.5375172691695909 1105 62-63 0.6821469414727019 1105 64-65 0.8441336464740985 1105 66-67 0.7152430096521201 1105 68-69 0.6714731531727693 1105 70-71 0.7833505223487194 1105 72-73 0.8799912881565852 1105 74-75 1.1095808111402796 1105 76-77 1.0738186999243666 1105 78-79 1.392001318145656 1105 80-81 1.4836452595437386 1105 82-83 1.221814736005733 1105 84-85 1.4512100872790903 1105 86-87 1.8403917230296685 1105 88-89 1.483821582006179 1105 90-91 1.6440147366586508 1105 92-93 1.6950785893521854 1105 94-95 1.5107137653596006 1105 96-97 1.6654258651422111 1105 98-99 2.1742246853894365 1105 100-101 2.4616090997044893 1106 1 -0.20973938207385956 1106 2 -0.45931989494398096 1106 3 -0.11326756830360551 1106 4 -0.14013154109592563 1106 5 -0.16637879425189794 1106 6 -0.02152023568938688 1106 7 -0.0769780725564857 1106 8 -0.16154208936833925 1106 9 -0.19931707584742497 1106 10-11 -0.3106565448815388 1106 12-13 -0.3884856670091281 1106 14-15 -0.4808434674110913 1106 16-17 -0.24433671670427515 1106 18-19 -0.20961481554157047 1106 20-21 -0.31706468975048097 1106 22-23 -0.5366478435975353 1106 24-25 -0.3832872778392655 1106 26-27 -0.22779279894564297 1106 28-29 -0.3709496982944458 1106 30-31 -0.0988382155052463 1106 32-33 -0.2537835322400568 1106 34-35 -0.2684259611502142 1106 36-37 -0.21584755076521134 1106 38-39 -0.3831725664377075 1106 40-41 -0.17200560564028677 1106 42-43 0.02590547669053933 1106 44-45 -0.05653876057553475 1106 46-47 0.039546946907634606 1106 48-49 0.19918697753075776 1106 50-51 0.430306031902596 1106 52-53 0.5625968398693502 1106 54-55 0.5353047054595663 1106 56-57 0.201438110466448 1106 58-59 0.25662614826653396 1106 60-61 0.5616495498190801 1106 62-63 0.21822432863194763 1106 64-65 0.2728354191539779 1106 66-67 0.5294095521815336 1106 68-69 0.40835494968699493 1106 70-71 0.7041757867610627 1106 72-73 0.16470006217713618 1106 74-75 0.4787432571405823 1106 76-77 0.8756211727383096 1106 78-79 1.0044510425609694 1106 80-81 0.9442720284994941 1106 82-83 1.126896294176447 1106 84-85 0.6942552980641317 1106 86-87 1.2435646710409891 1106 88-89 1.344372006555563 1106 90-91 1.0723813387739476 1106 92-93 1.10137048567276 1106 94-95 1.3273940871233414 1106 96-97 1.5747622274243511 1106 98-99 1.8013381220049602 1106 100-101 2.1969546977977394 1107 1 0.131367598615725 1107 2 0.20897884264396538 1107 3 0.2188328757860134 1107 4 0.228812124389826 1107 5 0.15408924626503762 1107 6 0.20000064650211868 1107 7 0.14952634339082493 1107 8 0.26097924190255384 1107 9 0.29640419554621644 1107 10-11 0.13035188783140228 1107 12-13 0.10932507097800226 1107 14-15 0.2883203717186902 1107 16-17 0.3220677790793758 1107 18-19 0.5020013712754547 1107 20-21 0.5821169878105081 1107 22-23 0.5080916903578583 1107 24-25 0.5243302312670721 1107 26-27 0.43719397816128946 1107 28-29 0.5897464995000803 1107 30-31 0.4804960243031431 1107 32-33 0.643152748384658 1107 34-35 0.6534841231769164 1107 36-37 0.6025653278336662 1107 38-39 0.7148390098309392 1107 40-41 0.5150858568615888 1107 42-43 0.7557218531188568 1107 44-45 0.8769663040419999 1107 46-47 0.6984306103974802 1107 48-49 0.6185429228428703 1107 50-51 0.5448680547557458 1107 52-53 0.6566165993623869 1107 54-55 1.0829507810046977 1107 56-57 1.1561715319524026 1107 58-59 0.9861836803258157 1107 60-61 1.4076652176999218 1107 62-63 1.3297675400485431 1107 64-65 1.3134396804183837 1107 66-67 1.3948603779886284 1107 68-69 1.3875444861372728 1107 70-71 1.4282227405058805 1107 72-73 1.122667478972705 1107 74-75 1.406974757564715 1107 76-77 1.3780412116585268 1107 78-79 1.5951650787867067 1107 80-81 1.6319217216285828 1107 82-83 1.919805205907398 1107 84-85 2.247767473105302 1107 86-87 1.9537088754220093 1107 88-89 1.8803981529554399 1107 90-91 2.00717126941613 1107 92-93 1.9058182427707102 1107 94-95 1.9590990918636244 1107 96-97 2.05513371587303 1107 98-99 2.1332109077940906 1107 100-101 2.937417998032263 1108 1 0.05289327098736152 1108 2 -0.06287771807323139 1108 3 0.11918821400931989 1108 4 -0.0491927655857225 1108 5 -0.067902603775714 1108 6 0.05154098880041147 1108 7 0.04398111111698455 1108 8 0.0842946453255422 1108 9 0.08134959081924364 1108 10-11 0.0056835911728825295 1108 12-13 -0.15143124524041696 1108 14-15 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-2.466082824320793 2316 88-89 -2.4042365277672566 2316 90-91 -2.0435741343243805 2316 92-93 -2.390628586683455 2316 94-95 -2.3326244657523496 2316 96-97 -2.5574270119077447 2316 98-99 -2.749334419739011 2316 100-101 -2.438713205727307 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1995.0 3 287.0 4 199.0 5 274.0 6 326.0 7 363.0 8 515.0 9 544.0 10 784.0 11 1112.0 12 1737.0 13 2391.0 14 2790.0 15 3847.0 16 5339.0 17 7547.0 18 6465.0 19 3921.0 20 3437.0 21 3368.0 22 3679.0 23 3979.0 24 4627.0 25 5087.0 26 6025.0 27 6977.0 28 8393.0 29 10088.0 30 12603.0 31 16033.0 32 20855.0 33 28054.0 34 39206.0 35 56367.0 36 85719.0 37 128904.0 38 107143.0 39 10931.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.58313192766064 20.366363863655305 15.472789399116593 22.577714809567464 2 14.129771984365785 20.53990023691234 42.87730206490265 22.45302571381922 3 17.956903431763767 25.18481739857744 31.544501963554694 25.3137772061041 4 11.339974309120263 17.54993643923074 40.324184914355406 30.785904337293594 5 12.21861791015433 37.878598550166856 36.43659604103251 13.4661874986463 6 29.26352071400027 37.71113627281204 19.328460103903026 13.69688290928467 7 25.992497889719314 33.618477900741055 22.874277218458587 17.514746991081047 8 24.00488193119341 35.5708296285936 22.041952639273237 18.382335800939757 9 25.046746711912043 16.69561105403637 22.866177026426254 35.39146520762533 10-11 22.89522848522389 27.15006227479503 29.91804370558342 20.036665534397667 12-13 24.157438543237237 25.014132536971466 29.815698397091406 21.012730522699893 14-15 21.115049935561647 26.67215030953721 28.846570114590374 23.36622964031077 16-17 19.81514225205786 29.515470577266314 29.831808252750474 20.83757891792536 18-19 19.292095951040565 28.110971172567535 32.760602703190905 19.836330173201 20-21 20.784596739018244 27.041276690672873 32.578852563270175 19.595274007038714 22-23 19.873648703771227 27.593914359494327 32.20917474572405 20.323262191010393 24-25 19.625561387370812 27.28598602306735 32.88616583351717 20.202286756044668 26-27 19.80787609764869 27.524050868961474 32.57847959214255 20.089593441247285 28-29 19.35012808782404 27.95020351685789 32.49875741906156 20.200910976256505 30-31 19.86267953896824 27.551176723291025 32.626888635054264 19.959255102686466 32-33 19.480569215950236 27.506720581171052 32.60328965059638 20.409420552282334 34-35 19.912584501648407 27.793383063039062 32.350477871390986 19.94355456392154 36-37 19.70987516607341 27.703625145262205 32.21099897109407 20.375500717570315 38-39 19.643476965259147 27.986042729159195 32.34569216593237 20.02478813964929 40-41 20.232512456321718 27.72503366910882 32.07929428882019 19.963159585749267 42-43 19.682989729340058 27.80570155649862 32.028675935622324 20.482632778539 44-45 20.065579720399636 28.008151264561477 31.62560789021099 20.300661124827894 46-47 19.960390236917664 28.096292183190574 31.165341207556658 20.777976372335104 48-49 20.045407128103214 28.066500545734733 31.220855642131223 20.667236684030826 50-51 20.242652090447592 28.50396178570894 30.67016531443708 20.583220809406388 52-53 20.249196543053635 28.51739296954357 30.58523304414434 20.648177443258454 54-55 20.39031594229924 28.151098205842 30.603996563528113 20.854589288330647 56-57 20.482319795919217 28.44821200431133 30.179902370816116 20.889565828953337 58-59 20.490439043388385 28.500288767685984 30.233227756982433 20.7760444319432 60-61 20.44338685902958 28.290298873305318 30.491669017935397 20.77464524972971 62-63 20.29463634025745 28.186033195483496 30.719429669534907 20.799900794724145 64-65 20.263891859769267 28.320059393563092 30.492885007370095 20.92316373929755 66-67 20.168111990318454 28.213566649271176 30.42137863817469 21.19694272223568 68-69 20.472808595248775 28.109423129901685 30.440673790533673 20.977094484315867 70-71 20.25089098593566 27.908542644975604 30.539635349128325 21.300931019960416 72-73 20.266854014422336 27.804327033931532 30.650337001209493 21.27848195043664 74-75 20.203003174994297 27.92588128151894 30.421839698583657 21.44927584490311 76-77 20.02362478054677 27.833620942976705 30.61681654833465 21.525937728141876 78-79 19.975460408682423 27.781597637273542 30.46738431930104 21.775557634743002 80-81 19.95068909574756 27.85574978961291 30.371821198860953 21.821739915778572 82-83 20.12812392581563 27.886554478469705 30.528600666004714 21.456720929709952 84-85 20.259452963226824 27.757488797597723 30.4378258351033 21.54523240407215 86-87 19.965607965767774 27.84015195086567 30.255501518593093 21.93873856477347 88-89 20.16173067638882 27.57046063693419 30.119945396127783 22.147863290549203 90-91 20.059696572940606 27.263571356632088 30.604065726159867 22.07266634426744 92-93 19.95865027437474 27.00799611139456 31.092197070395244 21.94115654383545 94-95 19.725725929021014 27.258108281325693 30.813882835896678 22.20228295375662 96-97 19.541580860135216 27.286144041766487 30.830917986991256 22.34135711110704 98-99 19.591572063815512 27.269094959120583 30.849519452477153 22.28981352458675 100-101 19.686781159598798 26.79695536240375 31.253557791855446 22.262705686142006 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 800.0 1 445.5 2 80.5 3 64.0 4 53.0 5 54.5 6 83.0 7 135.5 8 216.5 9 288.0 10 322.5 11 352.0 12 370.5 13 391.5 14 468.5 15 598.5 16 711.5 17 793.0 18 887.0 19 1039.5 20 1278.0 21 1542.5 22 1906.0 23 2440.0 24 3072.5 25 3960.0 26 5139.5 27 6506.5 28 7877.0 29 9322.5 30 11033.0 31 12979.0 32 15198.0 33 17284.5 34 19181.0 35 21063.5 36 22660.5 37 23725.5 38 24286.5 39 24209.5 40 24038.5 41 23973.0 42 23627.5 43 23389.5 44 23100.0 45 22771.0 46 22439.5 47 22352.5 48 22947.0 49 21940.5 50 19646.0 51 17573.0 52 15317.0 53 13503.0 54 11803.0 55 10208.5 56 8729.5 57 7313.0 58 5991.0 59 4724.0 60 3679.0 61 2825.5 62 2098.0 63 1452.0 64 990.5 65 744.5 66 590.0 67 445.0 68 328.0 69 221.0 70 157.0 71 135.0 72 113.0 73 86.5 74 59.0 75 54.0 76 43.0 77 26.5 78 18.0 79 9.5 80 6.0 81 5.0 82 4.5 83 3.0 84 2.0 85 1.0 86 0.0 87 1.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.5 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32579567411128885 2 0.2806062690331295 3 0.28642108218656914 4 0.2821015067011568 5 0.28309833181317506 6 0.22445178772276964 7 0.21265602389722071 8 0.22079676231203615 9 0.22162744990538466 10-11 0.22378723764809083 12-13 0.22370416888875597 14-15 0.22212586246139382 16-17 0.21381898652790862 18-19 0.20775496709646443 20-21 0.20484756051974462 22-23 0.2133205739718995 24-25 0.21240681761921615 26-27 0.2174740119386421 28-29 0.22370416888875597 30-31 0.2234549626107514 32-33 0.22104596859004072 34-35 0.22112903734937558 36-37 0.21149306126653275 38-39 0.20277084153637331 40-41 0.2013586726276808 42-43 0.21066237367318424 44-45 0.21066237367318424 46-47 0.2162279805486193 48-49 0.22337189385141656 50-51 0.21564649923327536 52-53 0.22943591328286075 54-55 0.21514808667726623 56-57 0.19413169056554871 58-59 0.17976079520061936 60-61 0.19463010312155785 62-63 0.18881528996811822 64-65 0.19479624064022755 66-67 0.19612534078958516 68-69 0.1928025904161911 70-71 0.19471317188089268 72-73 0.20692427950311593 74-75 0.21265602389722071 76-77 0.21107771746985848 78-79 0.20750576081845987 80-81 0.20476449176040976 82-83 0.1934671404908699 84-85 0.1905597339141501 86-87 0.19778671597628222 88-89 0.20285391029570818 90-91 0.20044491627499747 92-93 0.19629147830825489 94-95 0.199032747366305 96-97 0.20567824811309313 98-99 0.20634279818777193 100-101 0.18557560835405898 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 601911.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.7253034711042 #Duplication Level Percentage of deduplicated Percentage of total 1 87.80474317124356 71.75869281872244 2 9.18035259098432 15.005342029398625 3 1.891849944949419 4.6383603261834905 4 0.5607408007206931 1.8330684843011438 5 0.2141261312007918 0.8749761526739089 6 0.08785835253743096 0.43081503141556815 7 0.05484217572063765 0.31373954176493135 8 0.03740053602111875 0.24452561250463148 9 0.02791074899268965 0.20529129883800398 >10 0.12204518306931522 1.7644125058650115 >50 0.011611618548543421 0.6619420584779253 >100 0.005500191947319289 0.8510625274001524 >500 4.0742162568004726E-4 0.24855953703546485 >1k 6.111324385200709E-4 1.169212075418667 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3065 0.5092114947226417 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2371 0.3939120567658673 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1587 0.2636602421288197 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 917 0.15234810462011825 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.492062780045555E-4 0.0 12-13 0.0 0.0 0.0 4.1534379667425915E-4 0.0 14-15 0.0 0.0 0.0 4.98412556009111E-4 0.0 16-17 0.0 0.0 0.0 5.814813153439628E-4 0.0 18-19 0.0 0.0 0.0 6.645500746788146E-4 0.0 20-21 0.0 0.0 0.0 6.645500746788146E-4 0.0 22-23 0.0 0.0 0.0 6.645500746788146E-4 0.0 24-25 0.0 0.0 0.0 9.96825112018222E-4 0.0 26-27 0.0 0.0 0.0 0.001910581464701592 0.0 28-29 0.0 0.0 0.0 0.003405819132728925 0.0 30-31 0.0 0.0 0.0 0.00996825112018222 0.0 32-33 0.0 0.0 0.0 0.0203518460370387 0.0 34-35 0.0 0.0 0.0 0.02807724065517992 0.0 36-37 0.0 0.0 0.0 0.03306136621527103 0.0 38-39 0.0 0.0 0.0 0.03655025410733481 0.0 40-41 0.0 0.0 0.0 0.041368242148756215 0.0 42-43 0.0 0.0 0.0 0.04859522421088832 0.0 44-45 0.0 0.0 0.0 0.055822206273020425 0.0 46-47 0.0 0.0 0.0 0.0677841076172391 0.0 48-49 0.0 0.0 0.0 0.08456399700287917 0.0 50-51 0.0 0.0 0.0 0.09802113601512516 0.0 52-53 1.6613751866970366E-4 0.0 0.0 0.14005392823856017 0.0 54-55 1.6613751866970366E-4 0.0 0.0 0.20476449176040976 0.0 56-57 1.6613751866970366E-4 0.0 0.0 0.2872517697799176 0.0 58-59 1.6613751866970366E-4 0.0 0.0 0.3953242256745598 0.0 60-61 1.6613751866970366E-4 0.0 0.0 0.5588035440455483 0.0 62-63 1.6613751866970366E-4 0.0 0.0 0.6625564244547781 0.0 64-65 1.6613751866970366E-4 0.0 0.0 0.7667246486606824 0.0 66-67 1.6613751866970366E-4 0.0 0.0 0.9653420522303131 0.0 68-69 1.6613751866970366E-4 0.0 0.0 1.2172895992929187 0.0 70-71 1.6613751866970366E-4 0.0 0.0 1.4164884841778935 0.0 72-73 1.6613751866970366E-4 0.0 0.0 1.6176810192869047 0.0 74-75 1.6613751866970366E-4 0.0 0.0 2.0112608010154327 0.0 76-77 1.6613751866970366E-4 0.0 0.0 2.325011505023168 0.0 78-79 1.6613751866970366E-4 0.0 0.0 2.5309389594142653 0.0 80-81 3.322750373394073E-4 0.0 0.0 2.72482144370181 0.0 82-83 3.322750373394073E-4 0.0 0.0 3.09173615368385 0.0 84-85 3.322750373394073E-4 0.0 0.0 3.513143969789554 0.0 86-87 3.322750373394073E-4 0.0 0.0 4.040713660325197 0.0 88-89 3.322750373394073E-4 0.0 0.0 4.873311835138417 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 60 8.264335E-5 39.600937 2 GTATCAA 3190 0.0 36.66166 1 GGTATCA 1880 0.0 34.39131 1 TCACGCA 70 2.0468139E-4 33.94366 4 ATCAACG 3530 0.0 32.847466 3 TCAACGC 3580 0.0 32.3887 4 TATCAAC 3610 0.0 32.11954 2 CAACGCA 3610 0.0 32.11954 5 AACGCAG 3640 0.0 31.977377 6 TAGGACG 120 1.4979378E-7 31.68075 4 ATCACGC 65 0.0058291974 29.243769 3 CGCAGAG 4165 0.0 27.948946 8 ACGCAGA 4200 0.0 27.716036 7 GTACATG 2310 0.0 27.166248 1 TACATGG 2360 0.0 26.176891 2 GCAGAGT 4350 0.0 26.100609 9 ACATGGG 2460 0.0 25.11279 3 GAGTACT 2615 0.0 24.250202 12-13 AGAGTAC 3640 0.0 22.05419 10-11 GTACTTT 2885 0.0 21.977024 14-15 >>END_MODULE