FastQCFastQC Report
Thu 26 May 2016
SRR937708_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937708_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601911
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34270.5693532764810745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29430.48894271744493784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19820.32928456200335265No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7220.11995128847952605No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7040.11696081314347137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.029.371021
GTATCAA40550.027.6574841
ACGTGTC700.00838680427.143957
GTATACC1351.3366171E-524.6408561
TAGGACG1551.4072593E-624.5191524
TGAGCGG500.001654103323.74898354-55
ATCAACG48300.023.1136153
TCAACGC48600.022.970944
TATCAAC49150.022.713892
CAACGCA49400.022.598945
GAGTACT32050.022.37999712-13
GTGTAGA3400.022.363131
AACGCAG50150.022.3519826
AGTACTT33250.021.64372612-13
GTACTTT34500.020.85780114-15
GTACATG24500.020.5603871
AGAGTAC47050.020.2930610-11
ACGCAGA56000.020.0186617
GTGTAGC3800.020.0091151
AGGACGT1908.231753E-620.0024665
Produced by FastQC (version 0.11.5)