##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937708_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 601911 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46773027906119 33.0 31.0 34.0 31.0 34.0 2 32.69422721963878 34.0 31.0 34.0 31.0 34.0 3 32.797325518224454 34.0 31.0 34.0 31.0 34.0 4 36.33812640074695 37.0 37.0 37.0 35.0 37.0 5 36.231788420547225 37.0 35.0 37.0 35.0 37.0 6 36.314754174620504 37.0 37.0 37.0 35.0 37.0 7 36.283266130707034 37.0 37.0 37.0 35.0 37.0 8 36.27746793130546 37.0 37.0 37.0 35.0 37.0 9 37.99990197886398 39.0 38.0 39.0 35.0 39.0 10-11 37.95639720822514 39.0 38.0 39.0 35.0 39.0 12-13 37.90442274688451 39.0 38.0 39.0 35.0 39.0 14-15 39.2151314729254 40.0 38.5 41.0 36.0 41.0 16-17 39.19479457926504 40.0 38.5 41.0 36.0 41.0 18-19 39.197189451596664 40.0 38.5 41.0 36.0 41.0 20-21 39.145046360674584 40.0 39.0 41.0 36.0 41.0 22-23 39.002211290373495 40.0 38.0 41.0 35.5 41.0 24-25 38.96364080403913 40.0 38.0 41.0 35.5 41.0 26-27 38.91217223144285 40.0 38.0 41.0 35.0 41.0 28-29 38.811120747087195 40.0 38.0 41.0 35.0 41.0 30-31 38.854206851179 40.0 38.0 41.0 35.0 41.0 32-33 38.75118165310154 40.0 38.0 41.0 35.0 41.0 34-35 38.625671403247324 40.0 38.0 41.0 35.0 41.0 36-37 38.483795777116555 40.0 38.0 41.0 35.0 41.0 38-39 38.34916457748737 40.0 38.0 41.0 35.0 41.0 40-41 38.16535833370715 40.0 38.0 41.0 34.0 41.0 42-43 37.945031740572944 40.0 38.0 41.0 34.0 41.0 44-45 37.603860039108774 40.0 38.0 41.0 33.0 41.0 46-47 37.18236583149336 40.0 37.5 41.0 33.0 41.0 48-49 36.81036399068965 40.0 37.0 41.0 31.5 41.0 50-51 36.54925894359798 40.0 37.0 41.0 31.0 41.0 52-53 36.343518393915375 40.0 37.0 41.0 30.5 41.0 54-55 36.14861333320042 40.0 36.0 41.0 30.0 41.0 56-57 35.88242613941264 39.0 36.0 41.0 29.0 41.0 58-59 35.672915929431426 39.0 36.0 40.5 28.5 41.0 60-61 35.37656729981675 39.0 35.0 40.0 28.0 41.0 62-63 35.0526523024168 38.5 35.0 40.0 27.0 41.0 64-65 34.71522450993585 38.0 34.5 40.0 26.0 41.0 66-67 34.35176795240493 38.0 34.0 40.0 26.0 41.0 68-69 33.940598360887236 37.0 34.0 39.5 25.0 41.0 70-71 33.554596942072834 36.5 34.0 39.0 24.5 40.5 72-73 33.11684202481763 36.0 33.0 39.0 22.5 40.0 74-75 32.6419969065194 35.5 33.0 38.5 21.5 40.0 76-77 31.380780547290215 34.5 30.5 36.5 20.5 39.0 78-79 31.872135581506235 35.0 32.0 37.0 20.5 39.0 80-81 31.636948818014623 35.0 32.0 36.5 19.5 39.0 82-83 31.26945013465446 35.0 32.0 36.0 18.0 37.5 84-85 30.858912696395315 34.5 31.5 36.0 16.5 37.0 86-87 30.441940752038093 34.0 31.0 35.0 9.0 37.0 88-89 30.03304142971303 34.0 31.0 35.0 6.0 36.0 90-91 29.555562201056304 34.0 30.0 35.0 2.0 36.0 92-93 29.15350192968728 34.0 29.5 35.0 2.0 35.5 94-95 28.7608275974355 34.0 29.0 35.0 2.0 35.0 96-97 28.33068842403611 34.0 29.0 35.0 2.0 35.0 98-99 27.664194540388863 34.0 29.0 35.0 2.0 35.0 100-101 25.358907712269755 31.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1071863840727687 1101 2 0.03321511924379905 1101 3 0.04671920042566313 1101 4 0.02634944830646191 1101 5 -0.024306904635011506 1101 6 0.0168909198319227 1101 7 0.04357680841534517 1101 8 -0.0343004733938983 1101 9 0.1518888796022253 1101 10-11 0.24306236722531338 1101 12-13 0.14565985076234966 1101 14-15 0.024555424264612213 1101 16-17 -0.13060196025061543 1101 18-19 0.07488912506686773 1101 20-21 0.0817449625667237 1101 22-23 0.02717375118042753 1101 24-25 -0.01023286798527323 1101 26-27 -0.004864349800357104 1101 28-29 0.18309989532410498 1101 30-31 0.1203420578551615 1101 32-33 0.04989132269251684 1101 34-35 -0.05739486226900681 1101 36-37 -0.013175140773675764 1101 38-39 0.1368993283994584 1101 40-41 -0.1040967430368056 1101 42-43 -0.03065381103393605 1101 44-45 0.07737527317206627 1101 46-47 -0.3012560565603266 1101 48-49 -0.39830637861029317 1101 50-51 -0.16925644352662061 1101 52-53 -0.09564068699353356 1101 54-55 -0.0819671185147044 1101 56-57 -0.08715382200919208 1101 58-59 -0.02669142970128746 1101 60-61 -0.11702781572753196 1101 62-63 -0.16422235871066704 1101 64-65 -0.3059226373921504 1101 66-67 -0.07814176098528947 1101 68-69 -0.15883136504182005 1101 70-71 0.11493296944554743 1101 72-73 0.31172911210183685 1101 74-75 0.285252552874681 1101 76-77 0.07033447977479668 1101 78-79 0.003290115134042537 1101 80-81 0.03991198407549135 1101 82-83 -0.038418071140934984 1101 84-85 0.05392160602574947 1101 86-87 0.19947305019404737 1101 88-89 0.2583114555958339 1101 90-91 0.2340507262036482 1101 92-93 0.20354479009387916 1101 94-95 0.5368141145747742 1101 96-97 0.4756940322427745 1101 98-99 0.6133639433815432 1101 100-101 1.524684419466766 1102 1 0.12652165619574163 1102 2 0.10881129705850867 1102 3 0.010998386134012605 1102 4 0.07106901623334494 1102 5 -0.004381686521178096 1102 6 -0.06966495885524182 1102 7 0.0363063472603784 1102 8 0.0776313919987075 1102 9 0.13209660706380077 1102 10-11 0.1211803564234799 1102 12-13 0.10541473235770127 1102 14-15 0.11442247868919964 1102 16-17 0.1732119159438099 1102 18-19 0.06147824014787773 1102 20-21 0.023257218486953946 1102 22-23 0.1786901616498966 1102 24-25 0.06348129902760746 1102 26-27 -0.14776837971311352 1102 28-29 0.03577127047990558 1102 30-31 0.15152610438611447 1102 32-33 0.07516344566717947 1102 34-35 -0.08986681906169025 1102 36-37 -0.15893334600840348 1102 38-39 -0.1780778215797696 1102 40-41 -0.1953846532985395 1102 42-43 0.00855682461209284 1102 44-45 -0.024282391971269135 1102 46-47 -0.12526104201941024 1102 48-49 -0.3736449743726453 1102 50-51 -0.32459088473975584 1102 52-53 -0.42257878420998907 1102 54-55 -0.38455955723510726 1102 56-57 -0.2270167218845458 1102 58-59 -0.07452690955172159 1102 60-61 -0.12288988469305195 1102 62-63 0.008656743906705344 1102 64-65 -0.04241204329161263 1102 66-67 0.035568251478359514 1102 68-69 -0.24554096205054066 1102 70-71 -0.16004833508291227 1102 72-73 -0.09012797140459838 1102 74-75 -0.17581932082702423 1102 76-77 -0.21155999467472597 1102 78-79 -0.10299156330384562 1102 80-81 -0.2908233711384689 1102 82-83 -0.033565557638649324 1102 84-85 0.057295100001653054 1102 86-87 0.20794833062612028 1102 88-89 0.1662964992185998 1102 90-91 0.2676734931334366 1102 92-93 0.36393116317239915 1102 94-95 0.14906172570896814 1102 96-97 0.10095784611896974 1102 98-99 0.36433195335245827 1102 100-101 1.0566993758440013 1103 1 0.16516174543382078 1103 2 0.17505505281776124 1103 3 0.13117686229677616 1103 4 0.1418833137259483 1103 5 -0.029034676481892063 1103 6 0.020285087218766762 1103 7 0.10362289995449459 1103 8 0.15168735979540315 1103 9 0.21143508128660216 1103 10-11 0.10572567869905214 1103 12-13 -0.042902838814526945 1103 14-15 0.1681195542020788 1103 16-17 0.090120592407267 1103 18-19 0.13590280001984212 1103 20-21 0.12896845569535031 1103 22-23 0.08070771827242851 1103 24-25 0.13126053177410313 1103 26-27 0.13861505304308963 1103 28-29 -0.03493341936926697 1103 30-31 0.050050766105883326 1103 32-33 -0.12981060739068084 1103 34-35 -0.09538988031587792 1103 36-37 -0.050631294502508695 1103 38-39 -0.06531289602566659 1103 40-41 -0.042270535252079355 1103 42-43 -0.1490531467735252 1103 44-45 -0.30217753722564566 1103 46-47 -0.3166204677917577 1103 48-49 -0.34313040043598875 1103 50-51 -0.33793876776026366 1103 52-53 -0.38026441396894484 1103 54-55 -0.36991384211798106 1103 56-57 -0.3346747072378662 1103 58-59 -0.3216349167295647 1103 60-61 -0.3609799957556632 1103 62-63 -0.15041692600939172 1103 64-65 -0.28165933947474286 1103 66-67 -0.2254482866660723 1103 68-69 -0.44989519843446146 1103 70-71 -0.2008330482115781 1103 72-73 -0.22600071014071688 1103 74-75 -0.1263940334706959 1103 76-77 -0.08150325089471622 1103 78-79 -0.16937014616431156 1103 80-81 -0.012906886342889834 1103 82-83 0.07314378811041777 1103 84-85 0.015579206893356456 1103 86-87 -0.13939635146321194 1103 88-89 -0.20676911987058944 1103 90-91 -0.09690092851649723 1103 92-93 0.288379487533426 1103 94-95 0.3060547903580755 1103 96-97 0.4902628560612534 1103 98-99 0.4212571194613872 1103 100-101 1.446164432345288 1104 1 0.20944325015380372 1104 2 0.2029939463678545 1104 3 0.047212238861824574 1104 4 0.1241585521570272 1104 5 0.06834612824579267 1104 6 0.1371679935446366 1104 7 0.11635205927468917 1104 8 0.17173056719497026 1104 9 0.18126285967236555 1104 10-11 0.037798661103998654 1104 12-13 0.1299034534150394 1104 14-15 0.06275895886410154 1104 16-17 0.015130826805382469 1104 18-19 0.13778451064374764 1104 20-21 0.18921866139661603 1104 22-23 0.18670467024573867 1104 24-25 0.1842534345736695 1104 26-27 -0.03335120789556356 1104 28-29 0.057909797758050274 1104 30-31 0.11543945030680902 1104 32-33 -0.015288705778715439 1104 34-35 0.05631125904622536 1104 36-37 0.2764398031518809 1104 38-39 0.0747990541034298 1104 40-41 -0.08203674512007098 1104 42-43 -0.14674179409008303 1104 44-45 -0.32472361228330016 1104 46-47 -0.19162097444512227 1104 48-49 -0.08246838687389157 1104 50-51 -0.17254278775077836 1104 52-53 -0.026155252460014822 1104 54-55 -0.170139404199233 1104 56-57 -0.18943846490352456 1104 58-59 0.09627305069869863 1104 60-61 0.13614221309091334 1104 62-63 0.2493275180297232 1104 64-65 -0.04120266239127801 1104 66-67 -0.036433139756859134 1104 68-69 -0.0012708636703422371 1104 70-71 0.10524233370373537 1104 72-73 0.13653207033245707 1104 74-75 0.1817231615341015 1104 76-77 -0.3111297054965476 1104 78-79 0.052571390089774184 1104 80-81 0.142642792033552 1104 82-83 0.35187914076142945 1104 84-85 0.26458616628407583 1104 86-87 0.3254530508884699 1104 88-89 0.26142419473683276 1104 90-91 0.4557565697507471 1104 92-93 0.7814656956179924 1104 94-95 0.8883869766281762 1104 96-97 1.0440076324649858 1104 98-99 1.2145982755227855 1104 100-101 2.094338250930953 1105 1 0.14747307829491518 1105 2 0.217723957516597 1105 3 0.060411927538488897 1105 4 0.11600734998617668 1105 5 -0.08056844715545708 1105 6 0.007621726269348983 1105 7 0.17102821990069828 1105 8 0.1631008105740932 1105 9 0.2248376088256876 1105 10-11 0.1284242527235122 1105 12-13 -0.022755416144690344 1105 14-15 0.14728429595574966 1105 16-17 0.1894666352994676 1105 18-19 0.14517318099861853 1105 20-21 0.18839565291937532 1105 22-23 0.29126881757740364 1105 24-25 0.29905391417987914 1105 26-27 0.22892555434175677 1105 28-29 0.2008555896341946 1105 30-31 0.25458047136320516 1105 32-33 0.2562935740336556 1105 34-35 0.33674153734404655 1105 36-37 0.38023353040740204 1105 38-39 0.36032514846805697 1105 40-41 0.2939860992813763 1105 42-43 0.36495123689500275 1105 44-45 0.6234925891513114 1105 46-47 0.5079493783178677 1105 48-49 0.3907798934853943 1105 50-51 0.5524566863652112 1105 52-53 0.3499885452639546 1105 54-55 0.10887361668887507 1105 56-57 0.39153978453377647 1105 58-59 0.22463870836924826 1105 60-61 0.5661838631045839 1105 62-63 0.6573214808812864 1105 64-65 0.5112174558274418 1105 66-67 0.6525327619238652 1105 68-69 0.8252531481935961 1105 70-71 0.7256224426668965 1105 72-73 0.6859178378075867 1105 74-75 0.7571217816144582 1105 76-77 0.5503340400597345 1105 78-79 0.7879044850223593 1105 80-81 1.031381511821511 1105 82-83 0.9239489150520122 1105 84-85 0.7828790416074938 1105 86-87 0.7834454360879874 1105 88-89 0.9282635266537866 1105 90-91 0.760035687948438 1105 92-93 1.1385724921429485 1105 94-95 0.9492580509040316 1105 96-97 1.0329009621521905 1105 98-99 1.5278305690081382 1105 100-101 2.0600243192117347 1106 1 -0.11390410031376064 1106 2 -0.0819557683883616 1106 3 -0.1637799389891441 1106 4 -0.02273464316144924 1106 5 -0.16562728821013195 1106 6 -0.19396474773630956 1106 7 -0.0992495992561615 1106 8 -0.009884441921066411 1106 9 0.21252940087097727 1106 10-11 0.14585312208146917 1106 12-13 -0.10706943372252908 1106 14-15 -0.028438182816778124 1106 16-17 -0.02147668363394928 1106 18-19 -0.003940479063949454 1106 20-21 -0.0609246956580165 1106 22-23 0.06296180134025064 1106 24-25 -0.13001095994494705 1106 26-27 0.01850361184212801 1106 28-29 -0.10940993993528991 1106 30-31 -0.14778248111705494 1106 32-33 -0.052072655669903156 1106 34-35 -0.12697624904058813 1106 36-37 -0.20249773728215104 1106 38-39 -0.366659806097708 1106 40-41 -0.10787409735202402 1106 42-43 -0.09425105716130133 1106 44-45 0.10157615834540934 1106 46-47 -0.0051502045809854735 1106 48-49 -0.08661471399263831 1106 50-51 -0.12757645827951336 1106 52-53 0.04962916793638783 1106 54-55 -0.3235057219078712 1106 56-57 -0.1153963840942609 1106 58-59 -0.13338862665235496 1106 60-61 -0.08871148919692473 1106 62-63 -0.10782636657886968 1106 64-65 -0.003036603460508047 1106 66-67 0.15372447948680446 1106 68-69 -0.20655081426728827 1106 70-71 0.26072224901279384 1106 72-73 0.32939877032656995 1106 74-75 0.4722454223116088 1106 76-77 0.3977010890691197 1106 78-79 0.12395952747729666 1106 80-81 0.3985608653147352 1106 82-83 0.35962409753376434 1106 84-85 0.44882571224655265 1106 86-87 0.7179940730610248 1106 88-89 0.9200983992889711 1106 90-91 1.0073357028449053 1106 92-93 0.9623565678170145 1106 94-95 0.8737608812332454 1106 96-97 1.018600351396941 1106 98-99 1.2207957112694174 1106 100-101 1.882030128834856 1107 1 0.30666498306531764 1107 2 0.3409772189875184 1107 3 0.19633183188057757 1107 4 0.20733745902906264 1107 5 -0.03667913193050509 1107 6 0.09093870019543004 1107 7 0.21032202890611273 1107 8 0.19020537395638115 1107 9 0.3345854359989602 1107 10-11 0.17631567385034685 1107 12-13 0.21117815871298262 1107 14-15 0.2795367186352422 1107 16-17 0.25343786653819933 1107 18-19 0.222395532113147 1107 20-21 0.08312063641124467 1107 22-23 0.07821802607953998 1107 24-25 0.13567251393359925 1107 26-27 -0.009891303581248678 1107 28-29 -0.1795006290226695 1107 30-31 -0.006013732052636556 1107 32-33 0.07311134213459525 1107 34-35 0.12181685010616405 1107 36-37 0.2292733819234698 1107 38-39 -0.10288180100814515 1107 40-41 0.07113081757237438 1107 42-43 0.05113839446286761 1107 44-45 -0.005310973035257405 1107 46-47 -0.017484294696245684 1107 48-49 -0.0016763376641080185 1107 50-51 0.10259232072353797 1107 52-53 0.3722209797222291 1107 54-55 0.3037989285586704 1107 56-57 0.29897602746205365 1107 58-59 0.41373040433412456 1107 60-61 0.39629736764654666 1107 62-63 0.17054359998966362 1107 64-65 -0.0693288293973282 1107 66-67 0.1962123792921986 1107 68-69 0.14557108583748857 1107 70-71 -3.1138204354164145E-4 1107 72-73 0.10680820598347651 1107 74-75 0.18126692822449542 1107 76-77 0.27230814460526886 1107 78-79 0.20094233771630599 1107 80-81 0.14930309374645034 1107 82-83 0.06057153029659901 1107 84-85 0.14154937873026086 1107 86-87 0.19187885924934278 1107 88-89 0.49569031087023063 1107 90-91 0.6043617993470782 1107 92-93 0.7602552505174813 1107 94-95 0.6835664049097083 1107 96-97 0.966569514612349 1107 98-99 1.4110868644054904 1107 100-101 2.7200684003781888 1108 1 0.2567285527474681 1108 2 0.2754319867136772 1108 3 0.21679148958228467 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0.3027185206418537 2316 76-77 0.21101277738080526 2316 78-79 -0.14586885842252073 2316 80-81 -0.36767290091902893 2316 82-83 -0.08923953882987234 2316 84-85 -0.3062763285697727 2316 86-87 -0.5950857043044664 2316 88-89 -0.4457411183362616 2316 90-91 -0.3454008463202136 2316 92-93 -0.24717497207166872 2316 94-95 -0.6129042393604465 2316 96-97 -0.9477725614045447 2316 98-99 -0.7068480735172216 2316 100-101 -1.4303539156920486 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 7.0 5 14.0 6 16.0 7 46.0 8 74.0 9 181.0 10 345.0 11 607.0 12 1205.0 13 1755.0 14 2279.0 15 3386.0 16 5040.0 17 7879.0 18 7284.0 19 3893.0 20 2718.0 21 2412.0 22 2323.0 23 2542.0 24 2836.0 25 3333.0 26 3820.0 27 4435.0 28 5649.0 29 7150.0 30 8946.0 31 11520.0 32 15627.0 33 21487.0 34 31026.0 35 46940.0 36 78645.0 37 143125.0 38 155552.0 39 17811.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.817096847116396 21.106767197244718 15.421503703408199 21.654632252230687 2 14.860668769967653 21.883467821654694 41.323883431271405 21.93197997710625 3 18.71954491610886 25.937057139676796 31.17155194040315 24.171846003811197 4 12.056267454823056 18.61321690416025 39.123890409047185 30.206625231969507 5 12.5777217999887 38.81686733374763 35.4230292476563 13.182381618607373 6 29.531608493614502 38.79394129696915 18.32662968445501 13.347820524961332 7 26.586322562638 34.34394785940114 21.893934485330888 17.175795092629972 8 24.453116822919004 36.76590060656808 20.792608874069423 17.988373696443492 9 25.450440347493235 17.26202046481955 22.11838627305366 35.169152914633564 10-11 23.461691180257546 28.25093743094909 28.605890239586916 19.681481149206444 12-13 24.802951409080183 25.88813089608927 28.949362167335124 20.359555527495424 14-15 21.462060005548995 27.725361390637488 28.031386700027078 22.78119190378644 16-17 20.115515416731043 30.524612442703326 28.9056853920264 20.454186748539236 18-19 19.709059977305614 28.940906545984372 32.025498786365425 19.324534690344585 20-21 21.222572772386616 27.890419015435835 31.8800453887701 19.00696282340745 22-23 20.274011747632738 28.48996151408395 31.54408020488164 19.691946533401673 24-25 20.005549043828307 28.24803893634466 32.09349308155517 19.65291893827187 26-27 20.199470172928976 28.428237839023286 31.92802060826274 19.444271379784993 28-29 19.858894834035702 28.823051995251635 31.806064714321437 19.511988456391226 30-31 20.298866271921955 28.39420642357304 31.89764881011716 19.409278494387845 32-33 20.051635540802543 28.512936297891216 31.683089360387168 19.752338800919073 34-35 20.396453960801512 28.623085472769226 31.737084053954824 19.243376512474438 36-37 20.191689469041105 28.51775428593264 31.63997667429238 19.65057957073388 38-39 20.175324923452138 28.810239387550652 31.61979096577401 19.3946447232232 40-41 20.70330995778446 28.58412622464118 31.373492094346172 19.339071723228184 42-43 20.277063541844072 28.898267418241332 31.128151234324307 19.696517805590286 44-45 20.623979292619673 29.011764197697005 30.753633012189507 19.610623497493815 46-47 20.666261291121113 29.088436662563073 30.22955220954593 20.01574983676989 48-49 20.64599251384341 29.02447371787523 30.2371114666454 20.092422301635956 50-51 20.928509364341238 29.517569873286913 29.62381481647619 19.930105945895658 52-53 20.912743672024327 29.838073932918597 29.110806340809802 20.138376054247267 54-55 21.07728551230996 29.47179898689341 29.157632939089 20.293282561707628 56-57 21.128622005578897 29.669917977907033 28.909755761233804 20.291704255280266 58-59 21.172897654304375 29.601967732771122 28.992492245531317 20.232642367393186 60-61 21.172066966711025 29.450284178225683 29.32966833967148 20.04798051539181 62-63 21.0521156782315 29.386570439815852 29.35741330528932 20.20390057666333 64-65 20.986989770912974 29.539832300788653 29.17333293460329 20.299844993695082 66-67 20.791445911438736 29.349023360596497 29.2322286849717 20.62730204299307 68-69 21.116244760438004 29.404928635628856 29.144341937595424 20.334484666337715 70-71 20.957915705145776 29.277584227568525 29.200164143868445 20.56433592341725 72-73 20.98451513970931 29.248119114054333 29.113547612145 20.653818134091363 74-75 20.92203000111312 29.37269795700693 28.91008803627114 20.795184005608803 76-77 20.754480313534724 29.12357474776171 29.29461332323217 20.82733161547139 78-79 20.56317296078656 29.18587631726285 29.007029278414915 21.243921443535672 80-81 20.566661848678624 29.41921646223445 28.86091133074491 21.153210358342015 82-83 20.679320264391468 29.53877694441283 29.07259467977382 20.70930811142188 84-85 20.678555467502672 29.419133393475118 28.998140921166087 20.904170217856127 86-87 20.387233328515343 29.458674122918506 28.66113096454459 21.492961584021558 88-89 20.615838554204856 29.230235034747665 28.563442103566807 21.590484307480672 90-91 20.614245327613293 29.130338342522894 28.872305127184788 21.383111202679025 92-93 20.411282651946554 28.83356385431074 29.282809780735093 21.472343713007614 94-95 20.089681032577904 29.136865749255286 29.007527690970925 21.765925527195883 96-97 20.07796833751169 29.296690042215545 29.17491124103065 21.450430379242114 98-99 20.186107866803784 29.284576239929955 28.857601398218 21.671714495048256 100-101 19.696609685094842 30.164564197317524 28.348858153105848 21.789967964481782 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 58.0 1 34.5 2 19.5 3 34.5 4 37.0 5 32.5 6 54.0 7 114.0 8 195.0 9 262.5 10 291.5 11 315.0 12 363.5 13 434.0 14 487.5 15 584.5 16 732.5 17 859.5 18 1031.0 19 1250.0 20 1419.5 21 1676.5 22 2106.0 23 2585.0 24 3223.0 25 4079.0 26 5152.0 27 6442.5 28 7865.5 29 9456.5 30 11089.5 31 12883.0 32 15167.5 33 17266.5 34 19095.0 35 20997.0 36 22611.5 37 23643.5 38 23946.5 39 24006.5 40 24140.0 41 23932.5 42 23653.0 43 23463.5 44 23124.5 45 22773.0 46 22343.0 47 22456.5 48 22942.5 49 21591.0 50 19623.5 51 17945.5 52 15693.0 53 13679.5 54 11708.5 55 10118.5 56 8742.0 57 7282.0 58 6039.5 59 4781.0 60 3664.5 61 2825.5 62 2082.0 63 1429.5 64 972.5 65 715.5 66 578.0 67 462.0 68 326.0 69 222.0 70 161.5 71 127.5 72 105.5 73 96.5 74 71.5 75 53.0 76 34.5 77 20.5 78 19.0 79 16.0 80 10.5 81 4.5 82 2.0 83 1.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0503396681569202 2 0.0 3 0.0 4 0.0 5 0.014121689086924811 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004817988041421406 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.153437966742592E-4 24-25 9.137563526833701E-4 26-27 0.0027412690580501105 28-29 0.0032396816140592213 30-31 4.98412556009111E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.010549732435526184 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 8.306875933485183E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 8.306875933485183E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.306875933485183E-5 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.008389944692820034 92-93 0.0022428565020409996 94-95 0.0 96-97 0.0 98-99 3.322750373394073E-4 100-101 0.013124863974906588 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 601911.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.3807674334411 #Duplication Level Percentage of deduplicated Percentage of total 1 86.42359768555642 68.60371508638433 2 9.819857360914174 15.590156267925853 3 2.2400612920804646 5.334533533898787 4 0.7465401350090177 2.370437153475222 5 0.3040325253442963 1.2067167593278687 6 0.1500589058805758 0.7147074665413566 7 0.07576362181648671 0.42099221103308004 8 0.04867178155119643 0.30908826975094184 9 0.03146047725978931 0.22476211458339573 >10 0.14139970959302486 2.0364600451814643 >50 0.010121760004275329 0.5403350141193679 >100 0.00738040186654124 1.0113651392074046 >500 4.217372494948233E-4 0.23869690308879568 >1k 6.32605874242235E-4 1.398034035482203 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3427 0.5693532764810745 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2943 0.48894271744493784 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1982 0.32928456200335265 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 722 0.11995128847952605 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 704 0.11696081314347137 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.306875933485183E-5 0.0 18-19 0.0 0.0 0.0 1.6613751866970366E-4 0.0 20-21 0.0 0.0 0.0 1.6613751866970366E-4 0.0 22-23 0.0 0.0 0.0 3.322750373394073E-4 0.0 24-25 0.0 0.0 0.0 6.645500746788146E-4 0.0 26-27 0.0 0.0 0.0 0.0015783064273621847 0.0 28-29 0.0 0.0 0.0 0.0031566128547243695 0.0 30-31 0.0 0.0 0.0 0.009802113601512516 0.0 32-33 0.0 0.0 0.0 0.020102639759034143 0.0 34-35 0.0 0.0 0.0 0.02774496561784051 0.0 36-37 0.0 0.0 0.0 0.032895228696601324 0.0 38-39 0.0 0.0 0.0 0.036301047829330246 0.0 40-41 0.0 0.0 0.0 0.041119035870751657 0.0 42-43 0.0 0.0 0.0 0.04809681165487921 0.0 44-45 0.0 0.0 0.0 0.05548993123568102 0.0 46-47 0.0 0.0 0.0 0.06745183257989969 0.0 48-49 0.0 0.0 0.0 0.0844809282435443 0.0 50-51 0.0 0.0 0.0 0.09860261733046913 0.0 52-53 0.0 0.0 0.0 0.14071847831323903 0.0 54-55 0.0 0.0 0.0 0.20601052315043256 0.0 56-57 0.0 0.0 0.0 0.2889131449666147 0.0 58-59 0.0 0.0 0.0 0.39731787589859635 0.0 60-61 0.0 0.0 0.0 0.5623755006969469 0.0 62-63 0.0 0.0 0.0 0.6675405500148692 0.0 64-65 0.0 0.0 0.0 0.7718749117394432 0.0 66-67 0.0 0.0 0.0 0.9716552779397618 0.0 68-69 0.0 0.0 0.0 1.2252642001890646 0.0 70-71 0.0 0.0 0.0 1.428533454281447 0.0 72-73 0.0 0.0 0.0 1.63147043333649 0.0 74-75 0.0 0.0 0.0 2.029120584272426 0.0 76-77 0.0 0.0 0.0 2.3465263136908945 0.0 78-79 0.0 0.0 0.0 2.5560257247333906 0.0 80-81 0.0 0.0 0.0 2.753895509469008 0.0 82-83 0.0 0.0 0.0 3.1247144511397864 0.0 84-85 0.0 0.0 0.0 3.555093693253654 0.0 86-87 0.0 0.0 0.0 4.088395128183402 0.0 88-89 0.0 0.0 0.0 4.9351980608428825 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2880 0.0 29.37102 1 GTATCAA 4055 0.0 27.657484 1 ACGTGTC 70 0.008386804 27.14395 7 GTATACC 135 1.3366171E-5 24.640856 1 TAGGACG 155 1.4072593E-6 24.519152 4 TGAGCGG 50 0.0016541033 23.748983 54-55 ATCAACG 4830 0.0 23.113615 3 TCAACGC 4860 0.0 22.97094 4 TATCAAC 4915 0.0 22.71389 2 CAACGCA 4940 0.0 22.59894 5 GAGTACT 3205 0.0 22.379997 12-13 GTGTAGA 340 0.0 22.36313 1 AACGCAG 5015 0.0 22.351982 6 AGTACTT 3325 0.0 21.643726 12-13 GTACTTT 3450 0.0 20.857801 14-15 GTACATG 2450 0.0 20.560387 1 AGAGTAC 4705 0.0 20.29306 10-11 ACGCAGA 5600 0.0 20.018661 7 GTGTAGC 380 0.0 20.009115 1 AGGACGT 190 8.231753E-6 20.002466 5 >>END_MODULE