FastQCFastQC Report
Thu 26 May 2016
SRR937707_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937707_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148441
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26061.755579657911224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20331.3695677070351182No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14070.9478513348737884No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4980.3354868264158824No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.32201345989315616No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.25936230556247936No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.16235406659885074No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.13608100187953462No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.1333863285749894No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1810.12193396703067212No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.12126029870453581No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1590.1071132638556733No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGA250.00501104557.0046546
GGGATAG459.594034E-442.2256747
GGTATCA11850.042.134721
TAACGGC250.001613232138.00310550-51
GTATCAA19450.037.1614951
ATATAGG1106.8963345E-834.571622
GTGGTAC600.00393836831.6692568
ATCAACG23050.031.1407073
TCAACGC23150.031.0061914
ATGGGAT4350.030.5978745
CAACGCA23350.030.5370315
TATCAAC23650.030.3506682
AACGCAG23850.029.8766566
TTTAGGC650.00580632629.2529133
CTAGACT650.00580632629.2529134
TAGACTT650.00580632629.2529135
GAGTACT16050.028.56152512-13
ACGCAGA26150.027.248887
ATTAGAG700.0083375327.1634163
TGGGATA1405.7447505E-727.1450776
Produced by FastQC (version 0.11.5)