##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937707_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 148441 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.927816438854496 33.0 31.0 34.0 30.0 34.0 2 32.04273751861009 33.0 31.0 34.0 30.0 34.0 3 32.01028017865684 34.0 31.0 34.0 30.0 34.0 4 35.618326473144215 37.0 35.0 37.0 35.0 37.0 5 35.61487729131439 37.0 35.0 37.0 35.0 37.0 6 35.73561212872455 37.0 35.0 37.0 35.0 37.0 7 35.71020809614594 37.0 35.0 37.0 35.0 37.0 8 35.70190850236794 37.0 35.0 37.0 35.0 37.0 9 37.34736359900567 39.0 37.0 39.0 35.0 39.0 10-11 37.242345443644275 39.0 37.0 39.0 34.0 39.0 12-13 37.131772892933895 39.0 37.0 39.0 34.0 39.0 14-15 38.181651295801025 40.0 38.0 41.0 33.5 41.0 16-17 38.149241112630605 40.0 38.0 41.0 33.5 41.0 18-19 38.2414258863791 40.0 38.0 41.0 34.0 41.0 20-21 38.24898781333998 40.0 38.0 41.0 34.0 41.0 22-23 38.15567464514521 40.0 38.0 41.0 34.0 41.0 24-25 38.1465733860591 40.0 38.0 41.0 34.0 41.0 26-27 38.032443866586725 40.0 38.0 41.0 34.0 41.0 28-29 37.895365835584506 40.0 38.0 41.0 33.0 41.0 30-31 37.534471608248396 39.5 37.0 41.0 33.0 41.0 32-33 37.234099743332365 39.0 35.5 41.0 33.0 41.0 34-35 37.13401957680156 39.5 35.0 41.0 33.0 41.0 36-37 36.95319015635842 39.5 35.0 41.0 32.5 41.0 38-39 36.74975242689014 39.0 35.0 41.0 31.5 41.0 40-41 36.466636576148105 39.0 35.0 41.0 31.0 41.0 42-43 36.08616217891283 39.0 35.0 41.0 30.0 41.0 44-45 35.62938810705937 39.0 35.0 41.0 29.0 41.0 46-47 34.956999750742725 39.0 35.0 41.0 24.0 41.0 48-49 34.416023874805475 39.0 35.0 40.0 20.5 41.0 50-51 33.45533578997716 38.0 34.0 39.5 14.5 40.5 52-53 33.51272559468072 38.0 33.5 40.0 11.5 40.5 54-55 33.757647819672464 38.0 34.0 40.0 8.5 41.0 56-57 33.546968155698224 38.0 34.0 40.0 4.5 41.0 58-59 33.30308674827036 38.0 34.0 40.0 2.0 41.0 60-61 32.96486482845036 38.0 33.0 40.0 2.0 41.0 62-63 32.59102943256917 37.5 33.0 40.0 2.0 41.0 64-65 32.203818352072545 37.0 32.5 40.0 2.0 41.0 66-67 31.749796215331344 36.0 31.5 39.0 2.0 41.0 68-69 31.251055975101217 36.0 31.0 39.0 2.0 40.5 70-71 30.806209200961998 35.0 31.0 39.0 2.0 40.0 72-73 30.332633841054694 35.0 30.0 38.0 2.0 40.0 74-75 29.79965777649032 35.0 29.5 37.0 2.0 39.5 76-77 29.311042771202025 35.0 29.0 37.0 2.0 39.0 78-79 28.734544364427617 34.0 28.5 36.0 2.0 39.0 80-81 28.17682109390263 34.0 27.0 36.0 2.0 37.5 82-83 27.691365593063914 34.0 26.0 35.0 2.0 37.0 84-85 27.17193363019651 34.0 26.0 35.0 2.0 36.5 86-87 26.658914989793924 33.0 25.0 35.0 2.0 36.0 88-89 26.231691379066433 33.0 24.0 35.0 2.0 36.0 90-91 25.706543340451763 33.0 21.0 35.0 2.0 35.0 92-93 25.127569202578805 32.0 18.5 35.0 2.0 35.0 94-95 24.429029041841538 32.0 8.5 34.5 2.0 35.0 96-97 23.689701632298355 31.0 2.0 34.0 2.0 35.0 98-99 22.730670771552333 31.0 2.0 34.0 2.0 35.0 100-101 20.15430709844315 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20768823233857248 1101 2 0.015331578065605811 1101 3 0.05424619711944345 1101 4 0.3073967924151475 1101 5 -0.21796459314865757 1101 6 0.28051869775848104 1101 7 0.32885910646886884 1101 8 0.3760524089607955 1101 9 0.49432955506920706 1101 10-11 0.4611835548393941 1101 12-13 0.47945715642231335 1101 14-15 0.5654850670199139 1101 16-17 0.27538554485790456 1101 18-19 0.3662048169255243 1101 20-21 0.3821107930238554 1101 22-23 0.5357164869011797 1101 24-25 0.3913822926836019 1101 26-27 0.6584465617123243 1101 28-29 0.7698154554634584 1101 30-31 0.6210389671403789 1101 32-33 0.7725976996026489 1101 34-35 0.7461752576348673 1101 36-37 0.8518624565050956 1101 38-39 0.8163658150270692 1101 40-41 1.3258127176105816 1101 42-43 1.6965596795502975 1101 44-45 1.7399043684465312 1101 46-47 1.6820472870486611 1101 48-49 1.5919757176295732 1101 50-51 1.8311346426433133 1101 52-53 1.872856752404175 1101 54-55 1.9528796763896352 1101 56-57 2.2853694886323197 1101 58-59 2.287548262941712 1101 60-61 2.265556410336611 1101 62-63 2.6601258747434287 1101 64-65 2.4089458458813944 1101 66-67 2.394086536271768 1101 68-69 2.3886787144871313 1101 70-71 2.1522716155167565 1101 72-73 2.176952808373823 1101 74-75 1.9694547369822537 1101 76-77 1.8125100166712826 1101 78-79 1.758087246574405 1101 80-81 2.0537926220183493 1101 82-83 1.9523274804041222 1101 84-85 1.8747699286052146 1101 86-87 2.0628211759557615 1101 88-89 1.9258079518466076 1101 90-91 2.170119469677253 1101 92-93 2.200018501206742 1101 94-95 1.863659530193491 1101 96-97 1.4723432980944438 1101 98-99 1.790834236844404 1101 100-101 2.3253127120100174 1102 1 -0.19656053613433144 1102 2 -0.07780265838759348 1102 3 -0.19975626593474516 1102 4 -0.15211675438288097 1102 5 -0.2307416867447145 1102 6 -0.062153716034622164 1102 7 0.048994574449160666 1102 8 0.038767926202176284 1102 9 0.063910835857385 1102 10-11 -0.19991558309163793 1102 12-13 0.10499594952575819 1102 14-15 -0.2209989231278584 1102 16-17 0.09188800791209673 1102 18-19 -0.13772067568532265 1102 20-21 -0.17884979810914103 1102 22-23 -0.12661417812346087 1102 24-25 6.455869345245446E-5 1102 26-27 -0.12257252695762588 1102 28-29 0.14643183477380717 1102 30-31 -0.04321595896799835 1102 32-33 0.3431026257110332 1102 34-35 0.5715293216742836 1102 36-37 0.645735609214455 1102 38-39 0.5127174160122934 1102 40-41 0.26277392450713677 1102 42-43 0.20133184703798435 1102 44-45 0.5041439004662394 1102 46-47 0.7562467944378213 1102 48-49 1.05356746639805 1102 50-51 0.8898630909191709 1102 52-53 0.6163912351627943 1102 54-55 0.40508103106944304 1102 56-57 0.3066133309968535 1102 58-59 0.36051624323727793 1102 60-61 0.38424483398192777 1102 62-63 0.5154984116892365 1102 64-65 0.8343614862754869 1102 66-67 0.7703950338087218 1102 68-69 0.7608308080831954 1102 70-71 0.7392482165019807 1102 72-73 0.9710568724132358 1102 74-75 1.127782938952695 1102 76-77 1.4394342777550264 1102 78-79 1.1089954978059318 1102 80-81 1.0096115875355913 1102 82-83 0.860501741487866 1102 84-85 1.0540340911667911 1102 86-87 1.2209954370395053 1102 88-89 1.2006694050485756 1102 90-91 1.3523854795294703 1102 92-93 1.4524045849505853 1102 94-95 1.8158608848732918 1102 96-97 2.0102897266658744 1102 98-99 2.1140331284700196 1102 100-101 1.8501741652119854 1103 1 0.3985673298268644 1103 2 0.17710254443427687 1103 3 0.08452503917989063 1103 4 0.28797363362416917 1103 5 -0.16576152801455635 1103 6 -0.03977717282474913 1103 7 0.08018945981071823 1103 8 0.1286599015108152 1103 9 0.288337643617524 1103 10-11 0.12396558974786842 1103 12-13 0.327796875451682 1103 14-15 0.40570963066402044 1103 16-17 0.10158818427893834 1103 18-19 -0.004591266514239578 1103 20-21 -0.30373956883224906 1103 22-23 0.3163146137636659 1103 24-25 -0.16946806917250967 1103 26-27 -0.02857451108461362 1103 28-29 -0.1280666220163198 1103 30-31 0.08283121916251446 1103 32-33 0.3439844599261974 1103 34-35 0.2658910941610557 1103 36-37 0.16430924237141653 1103 38-39 0.0199098763297485 1103 40-41 0.4257754677170098 1103 42-43 0.613864915821047 1103 44-45 0.34891352127752384 1103 46-47 0.04581910484345997 1103 48-49 0.1465734187790062 1103 50-51 0.11266401684509475 1103 52-53 0.2058974080023006 1103 54-55 0.7619207577008424 1103 56-57 0.6712517789686387 1103 58-59 0.6202164196041196 1103 60-61 0.7486077087614724 1103 62-63 1.0083059513717814 1103 64-65 1.0598079148469175 1103 66-67 1.2324486052372912 1103 68-69 0.9815373777481007 1103 70-71 1.0541181459552398 1103 72-73 1.0261439535419825 1103 74-75 0.6566354521802111 1103 76-77 0.7493714470671975 1103 78-79 0.60703892814103 1103 80-81 0.6620035628442338 1103 82-83 0.6229852070963346 1103 84-85 0.7627807842884806 1103 86-87 0.6347134705492081 1103 88-89 1.221199607870446 1103 90-91 1.1739242802349388 1103 92-93 0.5838254403297718 1103 94-95 -0.07807099343358814 1103 96-97 0.27867045858826955 1103 98-99 0.4287652713271619 1103 100-101 0.9447784421079284 1104 1 0.5298050100841571 1104 2 0.5359053248056824 1104 3 0.1841723054938278 1104 4 0.30612904393644413 1104 5 0.3897940275410008 1104 6 0.27555636799899474 1104 7 0.634603818146644 1104 8 0.2780011194794838 1104 9 0.007188146781750504 1104 10-11 0.42311487909323375 1104 12-13 0.29262725204677054 1104 14-15 0.3827300684687742 1104 16-17 0.27466111715579444 1104 18-19 0.07853457641552097 1104 20-21 0.26810398340346353 1104 22-23 0.2968364521286446 1104 24-25 0.2511149788615157 1104 26-27 -0.11587609838619528 1104 28-29 0.20827532216875966 1104 30-31 -0.1821340261948805 1104 32-33 0.5952034660471597 1104 34-35 0.5690345737751201 1104 36-37 0.8182671218194955 1104 38-39 0.7822814916425429 1104 40-41 1.3440501850113407 1104 42-43 0.7278549562816821 1104 44-45 0.6258613374410302 1104 46-47 0.30404091208487927 1104 48-49 1.395873138666957 1104 50-51 1.2980826287342992 1104 52-53 1.7781559410451422 1104 54-55 2.2376703167837277 1104 56-57 1.9200587091481083 1104 58-59 1.6945498566826558 1104 60-61 2.063604077679411 1104 62-63 2.481228453706045 1104 64-65 2.385438166947754 1104 66-67 2.5559780170020012 1104 68-69 2.482600766066387 1104 70-71 2.1365696450734077 1104 72-73 2.5528583430014713 1104 74-75 2.097189451557739 1104 76-77 2.5149332273348612 1104 78-79 2.5597570524277806 1104 80-81 2.8827733522414754 1104 82-83 2.667880943168541 1104 84-85 2.645289343267631 1104 86-87 2.8456015294764825 1104 88-89 2.49386793446034 1104 90-91 1.897028336672328 1104 92-93 2.8916115177236925 1104 94-95 3.207798910083376 1104 96-97 3.478120609018813 1104 98-99 4.149353506621278 1104 100-101 5.002616392102741 1105 1 0.6835686431969741 1105 2 0.5087284966276044 1105 3 0.4562087796579597 1105 4 0.08335057084507724 1105 5 0.36851743179632024 1105 6 0.5269420286462321 1105 7 0.16240642855250798 1105 8 0.6391668623723916 1105 9 0.9157345440640654 1105 10-11 1.018501970099848 1105 12-13 0.5348496729300081 1105 14-15 0.4367708337110443 1105 16-17 0.56225274955343 1105 18-19 1.0545760568374831 1105 20-21 0.7392733021948033 1105 22-23 0.8083687246121087 1105 24-25 0.9214171422800774 1105 26-27 1.1918746614922213 1105 28-29 0.8482270475397655 1105 30-31 1.1909215790259253 1105 32-33 1.2040448749511512 1105 34-35 1.2905167836803528 1105 36-37 1.1859141806430245 1105 38-39 1.9022975831855433 1105 40-41 1.492494669187984 1105 42-43 1.8102794154270399 1105 44-45 1.8332187484905589 1105 46-47 2.0991419312159394 1105 48-49 2.0543748372187167 1105 50-51 2.4154614951744904 1105 52-53 1.7121359160138567 1105 54-55 1.5917736602487764 1105 56-57 1.952509987531812 1105 58-59 2.761732048708403 1105 60-61 2.6058443780548792 1105 62-63 2.6418756913245005 1105 64-65 2.5598079148469175 1105 66-67 2.2111720094926106 1105 68-69 1.9670903065634384 1105 70-71 2.2581501921858695 1105 72-73 1.946028376972503 1105 74-75 2.094775720107716 1105 76-77 2.4128860254753945 1105 78-79 2.41403919081505 1105 80-81 2.225702013067508 1105 82-83 1.9742460423997201 1105 84-85 1.8075010364555446 1105 86-87 1.2709099507173178 1105 88-89 1.3400596026169644 1105 90-91 1.6371499172194426 1105 92-93 1.1491656031876651 1105 94-95 1.6528194714994342 1105 96-97 1.9603752129880618 1105 98-99 2.0298291011143945 1105 100-101 0.8193515968229264 1106 1 0.628576826503032 1106 2 0.7519363526014189 1106 3 0.672084393405914 1106 4 0.5776222566061264 1106 5 0.8667171044640796 1106 6 0.865730899349991 1106 7 0.7823736953282818 1106 8 0.35078715697140694 1106 9 0.947976769923315 1106 10-11 0.39984403229297527 1106 12-13 0.8480247956796063 1106 14-15 0.9995367911578441 1106 16-17 0.8594704254945142 1106 18-19 0.8116955331058904 1106 20-21 1.1995842678249176 1106 22-23 0.9473212614369828 1106 24-25 0.46462499825388903 1106 26-27 1.1039041213940237 1106 28-29 0.7005183732353402 1106 30-31 0.6182538212517912 1106 32-33 0.4628828454912437 1106 34-35 1.1824153106852648 1106 36-37 0.9897603344891763 1106 38-39 1.8540979105177868 1106 40-41 2.0197450783532886 1106 42-43 1.4994774514335845 1106 44-45 0.9046590103563474 1106 46-47 1.0852303109213324 1106 48-49 1.4029836751892546 1106 50-51 0.8751996293807096 1106 52-53 0.36639123516279426 1106 54-55 -0.27221979310638034 1106 56-57 0.3780419024254229 1106 58-59 1.135790968512005 1106 60-61 1.8047805482676402 1106 62-63 1.1279640710298935 1106 64-65 1.302115607154608 1106 66-67 0.8862947590834445 1106 68-69 0.43476624764363336 1106 70-71 0.1871190956228581 1106 72-73 0.9853082460396081 1106 74-75 0.9324189279636848 1106 76-77 1.4740153217110716 1106 78-79 1.4691946736301098 1106 80-81 1.3610538952279008 1106 82-83 1.3128712469823753 1106 84-85 1.0707579922656905 1106 86-87 0.6772110963801659 1106 88-89 1.4768026468068172 1106 90-91 2.077248116892111 1106 92-93 1.8832081563791547 1106 94-95 1.606829291466699 1106 96-97 1.7463817596329037 1106 98-99 2.4633806559425473 1106 100-101 1.8147689454317657 1107 1 0.7543934756073476 1107 2 0.7241175960155388 1107 3 0.5618631078421608 1107 4 0.7251459657736703 1107 5 0.855547452198536 1107 6 0.6887024483489412 1107 7 0.7969153180890842 1107 8 0.4296069672980636 1107 9 0.2600947888906475 1107 10-11 0.5104868141686367 1107 12-13 0.771733963224392 1107 14-15 0.8515915856783991 1107 16-17 0.7626316478338211 1107 18-19 0.6927281990700678 1107 20-21 0.7285621392685471 1107 22-23 0.03046140896069005 1107 24-25 -0.32209786792103046 1107 26-27 0.6365700855668308 1107 28-29 0.5593171087464128 1107 30-31 0.6431221036543135 1107 32-33 0.6640419603489889 1107 34-35 -0.18565634368776074 1107 36-37 -1.1051817097679333 1107 38-39 -0.7251433961207852 1107 40-41 -0.9039640891914971 1107 42-43 -1.034663749830905 1107 44-45 -0.6707888294750504 1107 46-47 -0.5975937143684433 1107 48-49 0.6716875740301376 1107 50-51 0.5942723374945089 1107 52-53 0.07187068721759005 1107 54-55 -0.03238839163113738 1107 56-57 0.015028203795282025 1107 58-59 -0.4892880620464801 1107 60-61 0.518479178404629 1107 62-63 0.4314414156031319 1107 64-65 0.41939695594280835 1107 66-67 0.8077910709907137 1107 68-69 0.26722146366049415 1107 70-71 0.2177829523141135 1107 72-73 0.1997444947224345 1107 74-75 -0.3712691647655042 1107 76-77 -0.06328710189271902 1107 78-79 -0.30193282900424023 1107 80-81 -0.4959534809575601 1107 82-83 -0.6503531998037175 1107 84-85 -0.5555855272285122 1107 86-87 0.3505514546520345 1107 88-89 -0.7009634227400738 1107 90-91 -0.4924041486505679 1107 92-93 0.6571807591188801 1107 94-95 1.3295900923096902 1107 96-97 0.31908987343690853 1107 98-99 0.5212310247518168 1107 100-101 -0.794609347508171 1108 1 -0.003518664213149947 1108 2 -0.0019098012447358315 1108 3 0.2654530936711659 1108 4 -0.5417411386193365 1108 5 0.269104372368588 1108 6 0.09517387017227463 1108 7 0.3762728995723137 1108 8 0.4751903399952795 1108 9 0.8089847274830007 1108 10-11 0.2154938996669813 1108 12-13 -0.046404912543209775 1108 14-15 0.7098816187440562 1108 16-17 0.7581441655475629 1108 18-19 0.4006875755462076 1108 20-21 -0.28383748283820864 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0.2780299772095134 2315 100-101 0.15492731647248803 2316 1 -0.16378249203078354 2316 2 -0.3509030898353416 2316 3 -0.1935841662223794 2316 4 -0.22723060864942113 2316 5 0.007243691970529653 2316 6 -0.09864700462522791 2316 7 0.10885794932931958 2316 8 0.2596152416384214 2316 9 -0.34236912579170564 2316 10-11 -0.208829173695662 2316 12-13 -0.11826327866215536 2316 14-15 0.04718779786926319 2316 16-17 -0.469233756229805 2316 18-19 -0.6543344101052568 2316 20-21 -0.0959758767285166 2316 22-23 -0.07632355012728453 2316 24-25 -0.468415786464746 2316 26-27 -0.1441598951263714 2316 28-29 -0.09968813166914714 2316 30-31 0.034774213611107996 2316 32-33 -0.7590066743848496 2316 34-35 -0.39393987775046213 2316 36-37 -0.06408581935697555 2316 38-39 -0.1508582071900122 2316 40-41 0.15717409229237234 2316 42-43 0.17283831875418088 2316 44-45 0.07358002223038085 2316 46-47 0.039024838541365625 2316 48-49 0.44970540983045737 2316 50-51 0.8461340640735315 2316 52-53 0.8227670740889721 2316 54-55 0.9246310071001247 2316 56-57 0.7572365332979061 2316 58-59 0.7222132710416886 2316 60-61 0.8437067227642849 2316 62-63 0.4877988671063065 2316 64-65 0.6487340893435594 2316 66-67 1.0042606857742022 2316 68-69 0.9117925770189572 2316 70-71 0.5847959154473301 2316 72-73 0.8787000890958829 2316 74-75 1.0513657529507796 2316 76-77 1.3291005049841758 2316 78-79 1.1868508429641302 2316 80-81 1.3063817217637812 2316 82-83 1.2755454854955381 2316 84-85 0.6036955245320819 2316 86-87 0.7820123354095934 2316 88-89 0.7686973293055281 2316 90-91 0.9387349522044452 2316 92-93 0.644953122822109 2316 94-95 1.0212330085549794 2316 96-97 0.7345077276246457 2316 98-99 1.33983909683052 2316 100-101 1.5943765861132313 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 615.0 3 112.0 4 95.0 5 101.0 6 119.0 7 135.0 8 139.0 9 168.0 10 262.0 11 362.0 12 681.0 13 934.0 14 1111.0 15 1493.0 16 2165.0 17 3188.0 18 2663.0 19 1419.0 20 1287.0 21 1254.0 22 1343.0 23 1445.0 24 1591.0 25 1655.0 26 1882.0 27 2006.0 28 2304.0 29 2819.0 30 3274.0 31 4201.0 32 5182.0 33 6901.0 34 9520.0 35 14166.0 36 19236.0 37 27561.0 38 22477.0 39 2575.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.86268297790417 20.960300465424183 16.04801502327121 19.129001533400434 2 14.613540435590073 21.865270977545162 42.08948168158028 21.431706905284482 3 17.43323091467738 29.67012188945538 31.45288179086336 21.443765405003884 4 10.770965868417143 18.701460080770428 40.14074044059052 30.386833610221913 5 10.816195589774761 39.74065444230575 34.79417823253301 14.648971735386485 6 26.22663157184315 39.883242221772285 18.61037996895458 15.279746237429977 7 24.800064789131625 36.628805517874376 22.342127108177603 16.229002584816396 8 25.370525349603152 34.45615787484477 20.657631877328438 19.515684898223636 9 27.170758702606395 16.69118738780083 21.919500047241755 34.21855386235102 10-11 23.073055169341668 28.937244367651626 27.277912015226573 20.711788447780133 12-13 25.184660289180204 27.670192843042972 28.417269828415243 18.727877039361577 14-15 22.432527282666648 27.453415310688328 29.106235363195225 21.0078220434498 16-17 18.10125770670158 31.521970215939497 29.703407990335194 20.67336408702372 18-19 18.0519094890708 27.796411145828415 35.67697613054305 18.474703234557737 20-21 19.328683786984644 26.9714177742237 35.64899636606573 18.050902072725922 22-23 18.85908236699247 26.98849163104392 35.92999834678957 18.22242765517404 24-25 18.41914357342655 27.410914023301874 36.09091307854007 18.0790293247315 26-27 18.133262252933754 27.508114637388744 36.06036885328871 18.2982542563888 28-29 17.749005395665247 27.199686855700545 36.84237166314042 18.208936085493793 30-31 17.731920494043802 27.576350690109003 36.4688016738096 18.22292714203759 32-33 17.582643791423134 28.023973111240096 35.956629726118 18.436753371218767 34-35 18.101871536846936 27.676203203142403 35.954024850337795 18.26790040967287 36-37 18.29928644928392 27.383141310031885 35.5452843238137 18.772287916870496 38-39 17.69833879869911 28.06465325290474 35.793422668447974 18.44358527994818 40-41 17.525321025391875 27.898338023036906 36.08608811244492 18.4902528391263 42-43 17.848695376372024 28.07479864628217 35.132216932270246 18.944289045075564 44-45 17.8287144982943 28.300135983722445 34.69035865285009 19.180790865133165 46-47 18.2873127655195 28.579430611466734 34.02597577888532 19.107280844128454 48-49 18.18209426627794 28.82349368318756 33.40858168664291 19.58583036389159 50-51 18.485061179313092 29.02491040756963 32.84245905069143 19.647569362425845 52-53 18.4501110293671 29.43088168791636 32.67874378201797 19.440263500698574 54-55 18.46369780051082 29.15748666439486 32.649880055468024 19.7289354796263 56-57 18.42610429437504 29.448974565721247 32.200221989062484 19.924699150841228 58-59 18.567563190780152 28.759058582205366 32.70333733687805 19.970040890136435 60-61 18.651089512409882 29.04657964961524 32.75217008471174 19.55016075326314 62-63 18.817960681909426 28.904565743261706 33.02564413990412 19.251829434924748 64-65 18.780321585932807 28.381341808195515 33.27058156717476 19.567755038696923 66-67 18.65206141223859 28.822662209072845 32.557190231527244 19.96808614716132 68-69 18.473020253788096 28.74431912115497 32.81464561775235 19.96801500730459 70-71 18.650895399522273 28.514122616428928 32.852795508832536 19.98218647521626 72-73 18.523204188764286 28.575766163313226 32.617775258761455 20.28325438916103 74-75 18.306737289565127 28.797948787152933 32.520832630478054 20.374481292803885 76-77 18.370348878400307 28.462553178568655 32.96312165367215 20.203976289358888 78-79 18.211631178873994 27.93014679833869 32.962654313698366 20.895567709088947 80-81 18.202091015515617 28.5930684083924 32.566942522376024 20.637898053715954 82-83 18.644399460188936 28.299257759784073 32.39811066126856 20.658232118758434 84-85 18.03331781470761 28.113339945617337 32.07126422821826 21.782078011456793 86-87 17.99475108115584 28.28681495874404 32.232612112993436 21.485821847106685 88-89 18.46724217614098 27.68180330799877 32.32062557137701 21.53032894448324 90-91 18.068895253710096 27.723069553101837 32.826284237621486 21.381750955566574 92-93 18.307982417967946 27.875886776031493 32.36146390994437 21.45466689605619 94-95 17.86409470122492 27.60713430288064 32.565859385278664 21.96291161061577 96-97 18.328565452656296 27.713306435289514 32.15215836560649 21.805969746447705 98-99 18.23245050314911 28.15813354113746 31.501553470767423 22.107862484946008 100-101 18.636326839009122 27.603688812132333 31.81465540504075 21.9453289438178 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 303.0 1 212.0 2 157.5 3 198.5 4 158.0 5 138.5 6 273.5 7 640.0 8 1109.0 9 1252.5 10 952.0 11 679.0 12 637.0 13 624.0 14 619.5 15 623.0 16 626.0 17 637.0 18 638.0 19 669.0 20 689.5 21 738.0 22 839.5 23 940.5 24 1032.0 25 1181.0 26 1436.5 27 1689.5 28 1840.0 29 2032.5 30 2411.0 31 2806.0 32 3328.5 33 4157.5 34 4820.5 35 5173.5 36 6083.5 37 6791.5 38 6506.5 39 6248.0 40 5848.5 41 5456.5 42 5158.0 43 5022.5 44 5115.0 45 5059.5 46 4588.0 47 4178.0 48 4613.0 49 4402.0 50 3612.5 51 3085.0 52 2692.5 53 2521.5 54 2373.5 55 2141.0 56 1836.5 57 1571.5 58 1429.5 59 1287.0 60 1066.5 61 893.5 62 730.0 63 542.0 64 387.0 65 296.5 66 210.5 67 148.5 68 109.5 69 71.5 70 52.5 71 43.5 72 34.0 73 26.0 74 21.5 75 17.5 76 13.0 77 13.0 78 10.5 79 5.5 80 3.5 81 3.0 82 2.5 83 1.0 84 0.0 85 1.0 86 1.5 87 1.5 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27216200375906924 2 0.24656260736588947 3 0.23982592410452638 4 0.24723627569202578 5 0.2532992906272526 6 0.18256411638294 7 0.18054311140453108 8 0.18391145303521264 9 0.17986944307839478 10-11 0.1879534629920305 12-13 0.1775116039369177 14-15 0.1818904480568037 16-17 0.1849219555244171 18-19 0.17515376479544062 20-21 0.1714485890016909 22-23 0.16471190574032782 24-25 0.1721222573278272 26-27 0.16976441818635013 28-29 0.17953260891532663 30-31 0.18593245801362157 32-33 0.18525878968748524 34-35 0.18458512136134894 36-37 0.16066989578350993 38-39 0.15831205664203285 40-41 0.16437507157725967 42-43 0.17279592565396354 44-45 0.17616426728464507 46-47 0.178185272263054 48-49 0.17852210642612215 50-51 0.1818904480568037 52-53 0.18997446797043943 54-55 0.16673291071873675 56-57 0.158648890805101 58-59 0.16100672994657808 60-61 0.15662788582669207 62-63 0.16134356410964626 64-65 0.16033306162044178 66-67 0.15460688084828314 68-69 0.16605924239260045 70-71 0.16100672994657808 72-73 0.15831205664203285 74-75 0.15898572496816915 76-77 0.15999622745737363 78-79 0.16471190574032782 80-81 0.158648890805101 82-83 0.16235406659885074 84-85 0.15629105166362392 86-87 0.1482070317499882 88-89 0.1512385392176016 90-91 0.15528054917441947 92-93 0.14921753423919268 94-95 0.15325954419601054 96-97 0.1529227100329424 98-99 0.1512385392176016 100-101 0.1407966801624888 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 148441.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.96230152046942 #Duplication Level Percentage of deduplicated Percentage of total 1 83.32871880680382 46.63266887180765 2 8.486716182542645 9.498723398521971 3 2.579720961587076 4.331013668730337 4 1.2856472174405027 2.8779110892543165 5 0.8414488810776324 2.3544707998464034 6 0.570596236953931 1.9159127195316656 7 0.44058696777455425 1.7259382515612265 8 0.3057625404774229 1.368894038708982 9 0.2949284347124749 1.4854386591305637 >10 1.747902396744953 17.739034363821315 >50 0.09389558329621649 3.6580190109201633 >100 0.02046442200045744 2.3389764283452683 >500 0.0 0.0 >1k 0.003611368588316019 4.07299869982013 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2606 1.755579657911224 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2033 1.3695677070351182 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1407 0.9478513348737884 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 498 0.3354868264158824 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 478 0.32201345989315616 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 385 0.25936230556247936 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 241 0.16235406659885074 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 202 0.13608100187953462 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 198 0.1333863285749894 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 181 0.12193396703067212 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 180 0.12126029870453581 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 159 0.1071132638556733 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 6.7366832613631E-4 0.0 24-25 0.0 0.0 0.0 0.001684170815340775 0.0 26-27 0.0 0.0 0.0 0.00269467330454524 0.0 28-29 0.0 0.0 0.0 0.006399849098294945 0.0 30-31 0.0 0.0 0.0 0.012799698196589892 0.0 32-33 0.0 0.0 0.0 0.019873215621021144 0.0 34-35 0.0 0.0 0.0 0.027957235534656864 0.0 36-37 0.0 0.0 0.0 0.03705175793749706 0.0 38-39 0.0 0.0 0.0 0.043451607035792 0.0 40-41 0.0 0.0 0.0 0.056251305232381886 0.0 42-43 0.0 0.0 0.0 0.0771350233426075 0.0 44-45 0.0 0.0 0.0 0.11789195707385425 0.0 46-47 0.0 0.0 0.0 0.1610067299465781 0.0 48-49 0.0 0.0 0.0 0.2660989888238425 0.0 50-51 0.0 0.0 0.0 0.35940205199372144 0.0 52-53 0.0 0.0 0.0 0.45405245181587295 0.0 54-55 0.0 0.0 0.0 0.6194380258823371 0.0 56-57 0.0 0.0 0.0 0.7956022931669822 0.0 58-59 0.0 0.0 0.0 1.0030921376169657 0.0 60-61 0.0 0.0 0.0 1.242918061721492 0.0 62-63 0.0 0.0 0.0 1.579752224789647 0.0 64-65 0.0 0.0 0.0 1.81654664142656 0.0 66-67 0.0 0.0 0.0 2.1146448757418774 0.0 68-69 0.0 0.0 0.0 2.48112044516003 0.0 70-71 0.0 0.0 0.0 2.785281694410574 0.0 72-73 0.0 0.0 0.0 3.296259119784965 0.0 74-75 0.0 0.0 0.0 3.817678404214469 0.0 76-77 0.0 0.0 0.0 4.271057187704205 0.0 78-79 0.0 0.0 0.0 4.699173408963831 0.0 80-81 0.0 0.0 0.0 5.255286612189355 0.0 82-83 0.0 0.0 0.0 5.728538611300113 0.0 84-85 0.0 0.0 0.0 6.231768850923936 0.0 86-87 0.0 0.0 0.0 7.080254107692618 0.0 88-89 0.0 0.0 0.0 8.079977903678902 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAGCGA 25 0.005011045 57.004654 6 GGGATAG 45 9.594034E-4 42.225674 7 GGTATCA 1185 0.0 42.13472 1 TAACGGC 25 0.0016132321 38.003105 50-51 GTATCAA 1945 0.0 37.161495 1 ATATAGG 110 6.8963345E-8 34.57162 2 GTGGTAC 60 0.003938368 31.669256 8 ATCAACG 2305 0.0 31.140707 3 TCAACGC 2315 0.0 31.006191 4 ATGGGAT 435 0.0 30.597874 5 CAACGCA 2335 0.0 30.537031 5 TATCAAC 2365 0.0 30.350668 2 AACGCAG 2385 0.0 29.876656 6 TTTAGGC 65 0.005806326 29.252913 3 CTAGACT 65 0.005806326 29.252913 4 TAGACTT 65 0.005806326 29.252913 5 GAGTACT 1605 0.0 28.561525 12-13 ACGCAGA 2615 0.0 27.24888 7 ATTAGAG 70 0.00833753 27.163416 3 TGGGATA 140 5.7447505E-7 27.145077 6 >>END_MODULE