FastQCFastQC Report
Thu 26 May 2016
SRR937707_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937707_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148441
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24271.6349930275328246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20251.3641783604260278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14650.9869240977896941No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12360.8326540511044792No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.29843506847838536No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.27553034538975085No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.16100672994657808No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2210.14888070007612453No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.14281768514089774No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG2080.1401230118363525No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA2000.134733665227262No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG1940.13069165527044416No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.12530230866135367No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.11587095209544533No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA1710.11519728376930903No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG1650.11115527381249114No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA1640.11048160548635484No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA1580.10643959552953698No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT1570.10576592720340067No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTC200.00207114971.2444466
TGGGAGT704.287118E-640.711116
TACCTGG756.837674E-638.035482
GTATAGT500.001603908338.035481
GGTATCA12100.036.935281
AGGGTGT958.6375076E-734.997276
GGGAGTG550.002573407534.542767
CAGGGTG1001.2797336E-633.2810445
GTATCAA18700.032.289481
CTGGTCG600.00394147431.6641989
TGCGTAT300.003947779531.66419832-33
GGTGTGC1252.1340384E-730.3976278
GTGTAGG650.00580136429.258061
GCACAGG856.34596E-427.9672661
GTAGATA856.3836284E-427.9389956
ATAGTAG1056.822527E-527.1682013
AGATCCT700.00833041727.16825
CAAGACC700.00833041727.16824
GTGTGCT1056.869706E-527.140749
CGTAGCT700.00837163927.140748
Produced by FastQC (version 0.11.5)