##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937707_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 148441 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25487567451041 33.0 31.0 34.0 31.0 34.0 2 32.53378109821411 34.0 31.0 34.0 31.0 34.0 3 32.58142965892173 34.0 31.0 34.0 31.0 34.0 4 36.213431599086505 37.0 35.0 37.0 35.0 37.0 5 36.16458390875836 37.0 35.0 37.0 35.0 37.0 6 36.24299216523737 37.0 37.0 37.0 35.0 37.0 7 36.18845197755337 37.0 36.0 37.0 35.0 37.0 8 36.184767011809406 37.0 36.0 37.0 35.0 37.0 9 37.86137926853093 39.0 38.0 39.0 35.0 39.0 10-11 37.799297363935835 39.0 37.5 39.0 35.0 39.0 12-13 37.7080961459435 39.0 37.0 39.0 35.0 39.0 14-15 38.81694073739735 40.0 38.0 41.0 34.5 41.0 16-17 38.79865064234275 40.0 38.0 41.0 35.5 41.0 18-19 38.902142938945445 40.0 38.0 41.0 35.5 41.0 20-21 38.88947123773082 40.0 38.0 41.0 35.0 41.0 22-23 38.80983690489824 40.0 38.0 41.0 35.0 41.0 24-25 38.79280320127188 40.0 38.0 41.0 35.0 41.0 26-27 38.61470550589123 40.0 38.0 41.0 35.0 41.0 28-29 38.440060360682025 40.0 38.0 41.0 34.0 41.0 30-31 38.33106082551316 40.0 37.5 41.0 35.0 41.0 32-33 38.08096145943506 40.0 36.5 41.0 34.0 41.0 34-35 37.881996887652335 40.0 36.0 41.0 34.0 41.0 36-37 37.67682783058589 40.0 36.0 41.0 33.0 41.0 38-39 37.47950027283567 40.0 35.0 41.0 33.0 41.0 40-41 37.237353561347604 40.0 35.0 41.0 33.0 41.0 42-43 36.887433391044254 40.0 35.0 41.0 32.5 41.0 44-45 36.37015716682049 40.0 35.0 41.0 31.0 41.0 46-47 35.652508404012366 39.0 35.0 41.0 28.5 41.0 48-49 35.059559016713706 39.0 35.0 41.0 24.5 41.0 50-51 34.67743750042104 39.0 35.0 41.0 22.5 41.0 52-53 34.417734992353864 39.0 35.0 40.0 20.0 41.0 54-55 34.157426182793166 38.5 35.0 40.0 18.0 41.0 56-57 33.868085636717616 38.0 34.0 40.0 12.0 41.0 58-59 33.571378527495774 38.0 34.0 40.0 7.5 41.0 60-61 33.246693972689485 38.0 33.5 40.0 3.5 41.0 62-63 32.89908111640315 37.0 33.0 40.0 2.0 41.0 64-65 32.44165358627333 37.0 33.0 40.0 2.0 41.0 66-67 32.01226076353568 36.0 32.0 39.0 2.0 40.5 68-69 31.54972682749375 36.0 31.5 39.0 2.0 40.0 70-71 31.114742557649166 35.0 31.0 38.5 2.0 40.0 72-73 30.611862625554934 35.0 31.0 38.0 2.0 40.0 74-75 30.107810510573223 35.0 30.0 37.0 2.0 39.0 76-77 28.863592268982288 33.5 28.5 36.0 2.0 38.5 78-79 29.34723223368207 34.0 29.5 36.0 2.0 39.0 80-81 29.118225422895293 34.0 29.5 36.0 2.0 37.5 82-83 28.73001394493435 34.0 29.0 35.5 2.0 37.0 84-85 28.319147001165447 34.0 29.0 35.0 2.0 37.0 86-87 27.95966074063096 34.0 29.0 35.0 2.0 36.0 88-89 27.509205677676654 34.0 27.0 35.0 2.0 36.0 90-91 26.982144421015757 33.5 26.0 35.0 2.0 35.5 92-93 26.55684076501775 33.0 25.0 35.0 2.0 35.0 94-95 26.076831872595847 33.0 24.5 35.0 2.0 35.0 96-97 25.51788926240055 33.0 21.5 35.0 2.0 35.0 98-99 24.702572065669187 32.0 10.0 34.0 2.0 35.0 100-101 22.235986014645547 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11284248973922217 1101 2 0.34288907482778086 1101 3 0.15629053430713924 1101 4 0.19792413339336434 1101 5 0.02755596541734917 1101 6 0.17032707326302443 1101 7 0.10431713233585072 1101 8 0.1299113047490721 1101 9 0.1471357224933243 1101 10-11 0.08364984669588438 1101 12-13 0.21349524102107864 1101 14-15 0.21959218908825306 1101 16-17 0.2438480832448846 1101 18-19 0.29730120860886444 1101 20-21 0.22035597721260558 1101 22-23 -0.1441733479527798 1101 24-25 -0.015424900384914508 1101 26-27 -0.13214200810847387 1101 28-29 0.13334640725590674 1101 30-31 0.2713135884686366 1101 32-33 0.4938369971126093 1101 34-35 0.44459775283079495 1101 36-37 0.6267061647199839 1101 38-39 0.8782803194586819 1101 40-41 0.7231483734237756 1101 42-43 0.531796860231708 1101 44-45 0.45880343277590185 1101 46-47 1.2051510348964456 1101 48-49 1.271583865655657 1101 50-51 0.9849845369839159 1101 52-53 0.7183572577097479 1101 54-55 0.30568015789825864 1101 56-57 0.7953649415281774 1101 58-59 0.9720055365219693 1101 60-61 1.158665419808763 1101 62-63 0.40898083669407015 1101 64-65 0.45119536458868836 1101 66-67 0.8603474753761979 1101 68-69 0.8758510613793611 1101 70-71 0.7027254164442951 1101 72-73 0.858372203580128 1101 74-75 1.0692873180318259 1101 76-77 0.4811675639679436 1101 78-79 0.4071617209310183 1101 80-81 0.8276111727429694 1101 82-83 0.6124115721491599 1101 84-85 0.5413064463241106 1101 86-87 0.9052066278116833 1101 88-89 0.783325343516811 1101 90-91 0.6864124689632263 1101 92-93 0.3888211694543351 1101 94-95 -0.06879479010263267 1101 96-97 0.25587968491109336 1101 98-99 -0.06020486925429225 1101 100-101 -0.24391722665451 1102 1 0.2304533271776421 1102 2 -0.10430920103429031 1102 3 0.02159225844506807 1102 4 0.03874925654606898 1102 5 -0.1653319656171348 1102 6 0.18525933927287497 1102 7 -0.014987054856270277 1102 8 0.07803322593134254 1102 9 -0.13041969622589278 1102 10-11 -0.0901494143878594 1102 12-13 0.031998935602352674 1102 14-15 -0.050420126182679326 1102 16-17 -0.07334711379944991 1102 18-19 0.05161155343645163 1102 20-21 0.009687873764335109 1102 22-23 0.0568734500767718 1102 24-25 0.1329741143934129 1102 26-27 0.1777409968176329 1102 28-29 -0.09610248436971602 1102 30-31 -0.10799675635895056 1102 32-33 -0.018401303380002787 1102 34-35 0.1583446740130583 1102 36-37 -0.15269654463961757 1102 38-39 -0.33423507462998714 1102 40-41 -0.27939165120676535 1102 42-43 0.12731718042875428 1102 44-45 0.23520429484486982 1102 46-47 -0.2556710094385295 1102 48-49 0.04413620555713749 1102 50-51 0.19326655668834292 1102 52-53 0.126916371010239 1102 54-55 0.31791845839087074 1102 56-57 0.29382553266118805 1102 58-59 0.47239038873871664 1102 60-61 0.12425963163141773 1102 62-63 0.12249684654629078 1102 64-65 0.10960053700248196 1102 66-67 0.18269969212496662 1102 68-69 0.3156601746798522 1102 70-71 -0.09214835202860883 1102 72-73 0.13708217894958707 1102 74-75 0.07821588946039526 1102 76-77 0.374178647711787 1102 78-79 0.42979103127584395 1102 80-81 0.6318753353045494 1102 82-83 0.5535291829865976 1102 84-85 0.7689080473093313 1102 86-87 0.29052066722054803 1102 88-89 0.21497558982223097 1102 90-91 0.15054424236224406 1102 92-93 -0.12711171231905993 1102 94-95 -0.2943181891174085 1102 96-97 -0.05384937912831589 1102 98-99 0.26391766769151914 1102 100-101 0.04310555659672133 1103 1 0.14535632541397803 1103 2 0.19314450266941208 1103 3 0.03895336955618234 1103 4 0.07611698141379719 1103 5 -0.03608813845664116 1103 6 -0.08728254785234668 1103 7 0.15316770704849603 1103 8 0.22138640053451297 1103 9 0.28597139724853804 1103 10-11 0.2247205150654068 1103 12-13 0.3200831507699107 1103 14-15 0.07237528827939599 1103 16-17 -0.30909997976064574 1103 18-19 0.07090167689323579 1103 20-21 -0.10315934845788632 1103 22-23 -0.29200221129888604 1103 24-25 -0.07954793146196693 1103 26-27 0.13480669346667185 1103 28-29 0.171341298699069 1103 30-31 0.03783657697438514 1103 32-33 0.27092299115262364 1103 34-35 0.3763506263940144 1103 36-37 0.7215869650605597 1103 38-39 0.6605014773982347 1103 40-41 0.5828493099925325 1103 42-43 0.5516723391589125 1103 44-45 0.1725113936103 1103 46-47 0.6019954367695775 1103 48-49 0.6756121755747699 1103 50-51 -0.016665101159979656 1103 52-53 0.37283788776509397 1103 54-55 0.25004478114219353 1103 56-57 0.5815239453595993 1103 58-59 0.6271247361813934 1103 60-61 0.1081385998853932 1103 62-63 0.047044906511011675 1103 64-65 0.2851406604592732 1103 66-67 0.48571997431191605 1103 68-69 0.6908255185951973 1103 70-71 0.259345254673331 1103 72-73 -0.06587747713506786 1103 74-75 0.047682379760221494 1103 76-77 0.3294806759304798 1103 78-79 0.44406572263386934 1103 80-81 0.5713319049694547 1103 82-83 0.45556291314532515 1103 84-85 -0.054532208422589434 1103 86-87 -0.25241363613042367 1103 88-89 -0.3831284666151582 1103 90-91 -0.009122865221531384 1103 92-93 -0.1303359186682691 1103 94-95 0.04215667860745853 1103 96-97 0.7793737249281243 1103 98-99 1.1992406394728263 1103 100-101 1.4516979287307592 1104 1 0.24936089020285124 1104 2 0.36339037879763936 1104 3 -0.24442664911795475 1104 4 -0.04370872664720338 1104 5 0.09753568144169122 1104 6 0.17239169221404893 1104 7 0.1727755501857473 1104 8 0.3030857469397432 1104 9 0.20230089200940427 1104 10-11 -0.12770193539626717 1104 12-13 0.022282549047737632 1104 14-15 0.13760508390134873 1104 16-17 0.3306707433434042 1104 18-19 -0.037148950765228506 1104 20-21 -0.040600991781886364 1104 22-23 0.5500457189843431 1104 24-25 0.6581841984270298 1104 26-27 0.42065066068317947 1104 28-29 0.36516327193280773 1104 30-31 0.27593181506961884 1104 32-33 0.07022789830067211 1104 34-35 0.0920369009038211 1104 36-37 0.5802341276292893 1104 38-39 0.7751399253700129 1104 40-41 0.8604455336671819 1104 42-43 1.1471438611010285 1104 44-45 1.0415330763574744 1104 46-47 0.9653111334186164 1104 48-49 0.40377381059914796 1104 50-51 0.6740963886211162 1104 52-53 1.3742903205900703 1104 54-55 1.2130077441051554 1104 56-57 1.2654641881233673 1104 58-59 1.3441078257135075 1104 60-61 1.1041703459171401 1104 62-63 1.1726544095714928 1104 64-65 1.1203673437251673 1104 66-67 1.7693593559905132 1104 68-69 1.809095048629434 1104 70-71 1.765388412677268 1104 72-73 1.1385265066806802 1104 74-75 1.4978587466032565 1104 76-77 1.2977605804848942 1104 78-79 1.3108309472422306 1104 80-81 1.234159999170096 1104 82-83 1.296832754415167 1104 84-85 1.204701114536224 1104 86-87 1.3968757092373565 1104 88-89 0.8735890352003857 1104 90-91 1.2112060070681245 1104 92-93 0.6012233717145499 1104 94-95 1.0210704663447743 1104 96-97 1.6620119654095014 1104 98-99 1.4641540122293364 1104 100-101 0.9962305565967213 1105 1 0.524526275505913 1105 2 0.46816420322102914 1105 3 0.5342252371684708 1105 4 0.23726749362813848 1105 5 0.14384356629776107 1105 6 0.25222362498715967 1105 7 0.2985577779704798 1105 8 0.2686677244115856 1105 9 0.509112978546149 1105 10-11 0.3649227740619878 1105 12-13 0.2794343459366999 1105 14-15 0.4089263783765844 1105 16-17 0.384622111124564 1105 18-19 0.2151753832236878 1105 20-21 0.576509682274974 1105 22-23 0.1467290731770774 1105 24-25 0.43312609007730174 1105 26-27 0.336080662471133 1105 28-29 0.8624366493688882 1105 30-31 0.4332266478963689 1105 32-33 0.24741366622486538 1105 34-35 0.8852158746209682 1105 36-37 0.9925352182783129 1105 38-39 1.0281794390478254 1105 40-41 1.1426733183981028 1105 42-43 0.8572278947144696 1105 44-45 0.6038878054831684 1105 46-47 0.4805087018076719 1105 48-49 0.27043749735044287 1105 50-51 0.5520241554725374 1105 52-53 0.4888084682746765 1105 54-55 0.33534816963707215 1105 56-57 -0.18679756764276334 1105 58-59 0.3614956318998068 1105 60-61 0.6619211057955567 1105 62-63 0.6286708587043393 1105 64-65 0.4719675582790792 1105 66-67 0.06320881066600137 1105 68-69 0.3271533357740779 1105 70-71 0.7876483804941863 1105 72-73 1.043854595362962 1105 74-75 0.9309894456914023 1105 76-77 0.603187006374398 1105 78-79 0.7828495419141426 1105 80-81 1.0462465359124522 1105 82-83 0.633458894232799 1105 84-85 0.2017062995889667 1105 86-87 0.464817779682555 1105 88-89 0.35669291505019274 1105 90-91 0.5670526010248551 1105 92-93 0.5942503697478081 1105 94-95 0.4586263397579664 1105 96-97 0.7860062439719862 1105 98-99 1.6702151600927344 1105 100-101 1.4415193103961137 1106 1 0.6485851953095079 1106 2 0.4125177220426295 1106 3 0.45861148921429873 1106 4 0.17982068511749816 1106 5 0.12649495745979067 1106 6 0.029146701910235606 1106 7 -0.10324254936175947 1106 8 0.001728280876399424 1106 9 -0.015927937984130836 1106 10-11 0.238490695502243 1106 12-13 0.09539179274521103 1106 14-15 -0.028716829479385808 1106 16-17 0.010890523563190868 1106 18-19 0.1448807842056894 1106 20-21 0.06521671991818323 1106 22-23 0.8342086149119368 1106 24-25 0.6890180704373634 1106 26-27 0.394018469345113 1106 28-29 0.2492615266192999 1106 30-31 0.7352724744102801 1106 32-33 0.5624709493672455 1106 34-35 0.31737626741965386 1106 36-37 0.5422897190966438 1106 38-39 0.28063443086452367 1106 40-41 -0.042269398459509944 1106 42-43 -0.4120028745162969 1106 44-45 -0.14676685900128206 1106 46-47 0.30816827627575805 1106 48-49 -0.9348816515857266 1106 50-51 -0.19306586089406608 1106 52-53 -0.1347594531655787 1106 54-55 0.22070005179746488 1106 56-57 0.8951991590348101 1106 58-59 0.3679604436837707 1106 60-61 0.9624121041588936 1106 62-63 0.41803256083199614 1106 64-65 0.42450438315632866 1106 66-67 0.4537161756414534 1106 68-69 0.9304266581963354 1106 70-71 2.0678447798395183 1106 72-73 1.8237236624660689 1106 74-75 1.6154411641856719 1106 76-77 1.6201264499095842 1106 78-79 1.8052718005066133 1106 80-81 2.389945390249604 1106 82-83 2.38034923793165 1106 84-85 1.2328846956609816 1106 86-87 1.5414135243634064 1106 88-89 1.481733831580474 1106 90-91 1.260915121483123 1106 92-93 1.7782110898787415 1106 94-95 2.049706536157313 1106 96-97 1.677331939553003 1106 98-99 1.9893640962629497 1106 100-101 1.8665602269263886 1107 1 -0.11348013661883272 1107 2 -0.09960029692469874 1107 3 0.016883354335476497 1107 4 -0.05579575323866237 1107 5 -0.25137648616507846 1107 6 -0.001201032547086811 1107 7 0.09380697645488567 1107 8 0.29825093630316246 1107 9 0.1549888164942672 1107 10-11 0.0767414858078368 1107 12-13 -0.3210465634191664 1107 14-15 -0.49489175044882927 1107 16-17 0.05082729911639916 1107 18-19 0.0867142931624798 1107 20-21 0.4204327367780394 1107 22-23 0.041584800370323194 1107 24-25 0.5742656995206161 1107 26-27 0.6526064567002265 1107 28-29 0.4269222220882156 1107 30-31 0.3962840655588522 1107 32-33 0.3870231095358534 1107 34-35 0.26447848027529375 1107 36-37 0.29244778021361384 1107 38-39 0.3543962854482885 1107 40-41 1.090501939791281 1107 42-43 0.37777583991994845 1107 44-45 0.26324367840651774 1107 46-47 -0.4260782990667096 1107 48-49 0.655529307318389 1107 50-51 0.2489783962186749 1107 52-53 -0.04150339415610205 1107 54-55 -0.3006908860318305 1107 56-57 -0.5699220211548592 1107 58-59 -0.005379914588097279 1107 60-61 -0.41050675780105905 1107 62-63 -0.31347428848307146 1107 64-65 -0.25726590135368 1107 66-67 -0.9497611689317829 1107 68-69 -0.8223657548700487 1107 70-71 -0.663240573163641 1107 72-73 -0.08934272320305325 1107 74-75 -0.5813976134750263 1107 76-77 -0.7243414109966295 1107 78-79 -0.6023151331077194 1107 80-81 -0.42723058446061657 1107 82-83 -0.6327171477374733 1107 84-85 -0.609791805919631 1107 86-87 -0.5599563386502915 1107 88-89 -0.8439774434458691 1107 90-91 -1.0481470492228837 1107 92-93 0.04470213308211157 1107 94-95 -0.39455057659294823 1107 96-97 -0.925302412396416 1107 98-99 -0.5174852188055432 1107 100-101 -1.2865483083739235 1108 1 0.5568080069805958 1108 2 0.3687511437932969 1108 3 0.3588767412036873 1108 4 0.366027581669222 1108 5 0.19996975852079402 1108 6 0.20049948705612763 1108 7 0.40604127026688275 1108 8 0.22904923578355607 1108 9 0.3238598604243563 1108 10-11 0.37244295014415485 1108 12-13 0.2867711030900395 1108 14-15 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0.32127040322686184 2316 58-59 0.28128001002347247 2316 60-61 0.5859536632709279 2316 62-63 0.1079654467380422 2316 64-65 0.4273438255930877 2316 66-67 0.688512252048266 2316 68-69 0.7994509225226132 2316 70-71 1.0653150947595016 2316 72-73 1.0276472556514094 2316 74-75 0.9115691972264557 2316 76-77 1.3531156084020992 2316 78-79 1.168255796376517 2316 80-81 0.3891709968577608 2316 82-83 0.46749430762705657 2316 84-85 0.980122692563409 2316 86-87 1.2145678867795127 2316 88-89 1.119937238911394 2316 90-91 1.3069140889585995 2316 92-93 1.0903948792437355 2316 94-95 1.1904912547943844 2316 96-97 1.1929746860682329 2316 98-99 1.4054714788132863 2316 100-101 1.4228844396264435 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 4.0 6 5.0 7 16.0 8 47.0 9 78.0 10 140.0 11 240.0 12 471.0 13 698.0 14 853.0 15 1427.0 16 2198.0 17 3323.0 18 2966.0 19 1596.0 20 1139.0 21 1025.0 22 1116.0 23 1156.0 24 1274.0 25 1401.0 26 1556.0 27 1758.0 28 2040.0 29 2422.0 30 2964.0 31 3603.0 32 4579.0 33 6062.0 34 8642.0 35 13221.0 36 19634.0 37 31384.0 38 26808.0 39 2594.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.62593825380355 21.78904955058148 16.294284905736657 18.290727289878316 2 15.27206095351015 23.446352422848136 40.81486920729448 20.466717416347237 3 18.20184450387696 30.202572065669187 31.66982167999407 19.925761750459777 4 11.496823653842267 20.241038527091572 39.00606975161849 29.25606806744767 5 11.305256064690026 40.95485175202157 33.42385444743935 14.316037735849058 6 27.245841782256925 40.22675675857748 17.427125928820207 15.100275530345389 7 24.978274196482104 37.835907869119715 21.26366704616649 15.922150888231688 8 26.715664809587647 34.80709507481087 19.442067892293906 19.035172223307576 9 28.344594822185243 17.264098193895215 21.258951367883537 33.132355616036 10-11 23.63194804669869 30.032470813319772 26.265317533565526 20.070263606416017 12-13 25.72488176881931 28.227610180681495 27.81329578679314 18.23421226370606 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28.72353325563692 35.355797926448886 17.694909088459386 40-41 18.054891234783312 28.989349160261117 35.39332655164748 17.562433053308094 42-43 18.41205461362511 28.99049685193482 34.532034805574554 18.06541372886552 44-45 18.50836359226898 29.063062092009616 34.03372383640638 18.394850479315014 46-47 18.867428810099636 29.885947952385123 32.93598129896726 18.310641938547974 48-49 19.014962173523486 30.23760281862828 32.264333977809365 18.48310103003887 50-51 19.440383721478568 30.228845130388503 31.557992737855443 18.772778410277486 52-53 19.045950916525758 30.603404719720295 31.336692692719666 19.01395167103428 54-55 19.04325624322121 30.52525919388848 31.208358876590697 19.223125686299607 56-57 19.514150403190495 30.746896073187322 30.803821046745846 18.935132476876333 58-59 19.134538301412682 30.071543576235676 31.241705458734447 19.552212663617194 60-61 18.97959458640133 30.742517229067438 31.38856515383216 18.889323030699064 62-63 19.358196185689938 30.017313275981707 32.00598217473609 18.61850836359227 64-65 19.084013176952457 29.906158002169214 31.90425825748951 19.10557056338882 66-67 19.006541319446786 30.34235824334247 31.331303346110577 19.31979709110017 68-69 19.17866357677461 29.98699820130557 31.26494701598615 19.56939120593367 70-71 19.018667349317237 30.621593764525972 31.090466919516846 19.269271966639945 72-73 19.11668609077007 30.198530055712368 30.977290640725947 19.70749321279161 74-75 19.23525171617006 30.407704070977694 30.502354470799848 19.854689742052397 76-77 19.11129674416098 30.173267493482257 31.28111505581342 19.43432070654334 78-79 19.019341017643374 29.664311073086274 30.861756522793566 20.454591386476782 80-81 18.766378561179188 30.84154647300948 30.513469998181098 19.87860496763024 82-83 19.090413026050754 30.61822542289529 30.069522571257266 20.221838979796686 84-85 18.572698917415 30.241981662748163 30.139247243012374 21.046072176824463 86-87 18.794335796713845 30.57612115251177 29.73134107153684 20.898201979237545 88-89 18.59290896719909 29.950283277531142 30.301601309611225 21.155206445658546 90-91 18.471105840817675 29.94556026438307 30.602812270500806 20.980521624298447 92-93 18.748652654271087 29.798571813527353 29.80867690649421 21.644098625707358 94-95 18.640402584191698 29.934115237703868 29.650164038237413 21.775318139867018 96-97 19.36897487890812 30.011250261046474 29.078893297673826 21.540881562371585 98-99 19.222711012604503 30.538470348089113 28.54910097750591 21.68971766180047 100-101 18.949038685051864 30.646525150505504 28.422378913395747 21.982057251046886 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 641.0 1 394.0 2 180.5 3 222.0 4 196.0 5 194.5 6 312.0 7 609.5 8 886.0 9 972.0 10 903.5 11 769.5 12 759.5 13 785.0 14 760.0 15 752.0 16 752.5 17 738.0 18 722.5 19 709.0 20 699.0 21 745.0 22 829.0 23 897.5 24 986.0 25 1138.0 26 1353.0 27 1594.5 28 1768.0 29 2049.0 30 2447.0 31 2796.0 32 3307.5 33 3876.0 34 4418.5 35 4886.5 36 5705.0 37 6275.5 38 6357.5 39 6371.5 40 5901.5 41 5515.0 42 5158.0 43 4980.0 44 5136.5 45 5043.0 46 4528.0 47 4204.0 48 4672.0 49 4473.0 50 3678.0 51 3151.5 52 2748.0 53 2517.5 54 2287.0 55 2146.5 56 1926.0 57 1726.5 58 1613.0 59 1427.0 60 1201.5 61 1022.0 62 807.0 63 558.0 64 378.5 65 296.0 66 236.5 67 185.0 68 126.5 69 71.0 70 50.0 71 37.0 72 39.5 73 40.0 74 24.0 75 17.5 76 19.0 77 13.0 78 11.0 79 10.0 80 6.5 81 6.5 82 4.5 83 2.5 84 4.0 85 3.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01818904480568037 2 0.0 3 0.0 4 0.0 5 0.02762040137158871 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00202100497840893 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001010502489204465 24-25 0.0 26-27 6.7366832613631E-4 28-29 6.7366832613631E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.00134733665227262 42-43 0.011115527381249116 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0134733665227262 92-93 6.7366832613631E-4 94-95 0.0 96-97 0.0 98-99 0.00134733665227262 100-101 0.016504873990339595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 148441.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.03723364838555 #Duplication Level Percentage of deduplicated Percentage of total 1 82.04346555906972 43.51358452179654 2 9.057653469496628 9.607857667356054 3 2.9468175640488257 4.688731549908718 4 1.4568964422258635 3.0907902803133904 5 0.8700732893851059 2.3073140170168616 6 0.6452514321279326 2.053341058063473 7 0.45853497440587326 1.7023598601464556 8 0.37089255547511085 1.5736892098544204 9 0.26419743677679125 1.2611071065271724 >10 1.7020411792350976 16.797246043882755 >50 0.13463907835740324 4.770245417371211 >100 0.044456299457633144 3.814983730909924 >500 0.0 0.0 >1k 0.005080719938015217 4.8187495368530255 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2427 1.6349930275328246 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2025 1.3641783604260278 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1465 0.9869240977896941 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1236 0.8326540511044792 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 443 0.29843506847838536 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 409 0.27553034538975085 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 239 0.16100672994657808 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 221 0.14888070007612453 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 212 0.14281768514089774 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 208 0.1401230118363525 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 200 0.134733665227262 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 194 0.13069165527044416 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 186 0.12530230866135367 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 172 0.11587095209544533 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 171 0.11519728376930903 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 165 0.11115527381249114 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 164 0.11048160548635484 No Hit GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA 158 0.10643959552953698 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 157 0.10576592720340067 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.001010502489204465 0.0 26-27 0.0 0.0 0.0 0.00202100497840893 0.0 28-29 0.0 0.0 0.0 0.005726180772158636 0.0 30-31 0.0 0.0 0.0 0.01212602987045358 0.0 32-33 0.0 0.0 0.0 0.019199547294884836 0.0 34-35 0.0 0.0 0.0 0.027620401371588714 0.0 36-37 0.0 0.0 0.0 0.036041255448292585 0.0 38-39 0.0 0.0 0.0 0.04244110454658753 0.0 40-41 0.0 0.0 0.0 0.054230300253972956 0.0 42-43 0.0 0.0 0.0 0.07545085252726672 0.0 44-45 0.0 0.0 0.0 0.11553411793237717 0.0 46-47 0.0 0.0 0.0 0.15864889080510103 0.0 48-49 0.0 0.0 0.0 0.265088486334638 0.0 50-51 0.0 0.0 0.0 0.35906521783065326 0.0 52-53 0.0 0.0 0.0 0.45708395928348633 0.0 54-55 0.0 0.0 0.0 0.6217958650238141 0.0 56-57 0.0 0.0 0.0 0.7999811372868681 0.0 58-59 0.0 0.0 0.0 1.0111761575306013 0.0 60-61 0.0 0.0 0.0 1.2536967549396731 0.0 62-63 0.0 0.0 0.0 1.590867752170896 0.0 64-65 0.0 0.0 0.0 1.829009505460082 0.0 66-67 0.0 0.0 0.0 2.128455076427672 0.0 68-69 0.0 0.0 0.0 2.496277982498097 0.0 70-71 0.0 0.0 0.0 2.8041444075423905 0.0 72-73 0.0 0.0 0.0 3.3232058528304176 0.0 74-75 0.0 0.0 0.0 3.8533828254996934 0.0 76-77 0.0 0.0 0.0 4.313161458087725 0.0 78-79 0.0 0.0 0.0 4.745993357630304 0.0 80-81 0.0 0.0 0.0 5.313895756563214 0.0 82-83 0.0 0.0 0.0 5.796242278076812 0.0 84-85 0.0 0.0 0.0 6.310588045081884 0.0 86-87 0.0 0.0 0.0 7.169851995068748 0.0 88-89 0.0 0.0 0.0 8.18304915757776 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCCTC 20 0.002071149 71.244446 6 TGGGAGT 70 4.287118E-6 40.71111 6 TACCTGG 75 6.837674E-6 38.03548 2 GTATAGT 50 0.0016039083 38.03548 1 GGTATCA 1210 0.0 36.93528 1 AGGGTGT 95 8.6375076E-7 34.99727 6 GGGAGTG 55 0.0025734075 34.54276 7 CAGGGTG 100 1.2797336E-6 33.281044 5 GTATCAA 1870 0.0 32.28948 1 CTGGTCG 60 0.003941474 31.664198 9 TGCGTAT 30 0.0039477795 31.664198 32-33 GGTGTGC 125 2.1340384E-7 30.397627 8 GTGTAGG 65 0.005801364 29.25806 1 GCACAGG 85 6.34596E-4 27.967266 1 GTAGATA 85 6.3836284E-4 27.938995 6 ATAGTAG 105 6.822527E-5 27.168201 3 AGATCCT 70 0.008330417 27.1682 5 CAAGACC 70 0.008330417 27.1682 4 GTGTGCT 105 6.869706E-5 27.14074 9 CGTAGCT 70 0.008371639 27.14074 8 >>END_MODULE