FastQCFastQC Report
Thu 26 May 2016
SRR937706_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937706_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137901
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24851.802017389286517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18861.3676478053096062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13570.9840392745520337No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4930.3575028462447698No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4340.3147185299598988No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3720.2697587399656275No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2400.17403789675201775No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.14430642272354807No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.13270389627341353No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.1283529488546131No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1550.11239947498567814No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGA250.004992415757.056111
TTAGGCA352.765818E-454.3391574
AGGCTTA654.3724867E-851.166966
TACCTGG554.9223927E-543.2086072
ACCCGGG554.9329785E-543.1928947
CGACCCG459.5662393E-442.2484135
GGTATCA10950.037.7768751
CATGGGT905.606071E-736.9808164
GACCCGG651.3201185E-436.5478326
ACTATAC550.002563955334.5668873
CTAGATC702.03219E-433.961974
TATACAA851.614945E-533.550215
CATGGGG1850.033.4112364
GTATCAA16650.033.125671
ATATAGG1151.01956175E-733.0639762
CTATACA600.00392003431.6978384
ATGTTAT600.00392003431.6978381
CCCGACC600.003927027331.686313
CCGGGGA753.05987E-431.6747889
ACAATCA600.0039340331.6747888