##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937706_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 137901 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.958223653200484 33.0 31.0 34.0 30.0 34.0 2 32.067345414464 33.0 31.0 34.0 30.0 34.0 3 32.04680894264726 34.0 31.0 34.0 30.0 34.0 4 35.64286698428583 37.0 35.0 37.0 35.0 37.0 5 35.667457088781084 37.0 35.0 37.0 35.0 37.0 6 35.76891393100848 37.0 35.0 37.0 35.0 37.0 7 35.73925497277032 37.0 35.0 37.0 35.0 37.0 8 35.73913169592679 37.0 35.0 37.0 35.0 37.0 9 37.3747978622345 39.0 37.0 39.0 35.0 39.0 10-11 37.28693410490134 39.0 37.0 39.0 35.0 39.0 12-13 37.186155285313376 39.0 37.0 39.0 34.0 39.0 14-15 38.25429474768131 40.0 38.0 41.0 33.5 41.0 16-17 38.24021943278149 40.0 38.0 41.0 33.5 41.0 18-19 38.33437393492433 40.0 38.0 41.0 34.0 41.0 20-21 38.14724693802076 40.0 38.0 41.0 34.0 41.0 22-23 38.104125423310926 40.0 38.0 41.0 34.0 41.0 24-25 37.99555115626428 40.0 38.0 41.0 33.5 41.0 26-27 38.016776528089 40.0 38.0 41.0 34.0 41.0 28-29 37.94041740088905 40.0 38.0 41.0 34.0 41.0 30-31 37.609654752322314 39.5 37.0 41.0 33.0 41.0 32-33 37.22961037265865 39.0 35.5 41.0 33.0 41.0 34-35 36.91007679422194 39.0 35.0 41.0 32.5 41.0 36-37 36.838964184451164 39.0 35.0 41.0 32.0 41.0 38-39 36.74494021073089 39.0 35.0 41.0 31.5 41.0 40-41 36.47070362071341 39.0 35.0 41.0 31.0 41.0 42-43 36.12444797354624 39.0 35.0 41.0 30.0 41.0 44-45 35.67021268881299 39.0 35.0 41.0 29.0 41.0 46-47 35.02747985873924 39.0 35.0 41.0 24.5 41.0 48-49 34.44179882669451 39.0 35.0 40.5 20.0 41.0 50-51 33.46465580380128 38.0 34.0 39.5 14.0 40.5 52-53 33.51384326437082 38.0 33.5 40.0 10.0 40.5 54-55 33.77538959108345 38.0 34.0 40.0 7.5 41.0 56-57 33.535710400939806 38.0 34.0 40.0 2.0 41.0 58-59 33.316748972088675 38.0 34.0 40.0 2.0 41.0 60-61 33.005761379540395 38.0 33.5 40.0 2.0 41.0 62-63 32.66439692242986 37.5 33.0 40.0 2.0 41.0 64-65 32.27451215002067 37.0 33.0 40.0 2.0 41.0 66-67 31.842568219229737 36.5 32.0 39.5 2.0 41.0 68-69 31.381498321259457 36.0 31.5 39.0 2.0 41.0 70-71 30.92416298649031 35.5 31.0 39.0 2.0 40.0 72-73 30.45557320106453 35.0 30.5 38.0 2.0 40.0 74-75 29.947951791502597 35.0 30.0 37.5 2.0 39.5 76-77 29.48953234566827 35.0 29.0 37.0 2.0 39.0 78-79 28.968945112798313 34.5 29.0 36.5 2.0 39.0 80-81 28.421204342245524 34.0 28.5 36.0 2.0 38.5 82-83 27.910526392121884 34.0 26.5 35.5 2.0 37.0 84-85 27.44632743779958 34.0 26.0 35.0 2.0 37.0 86-87 26.968303348054036 34.0 25.5 35.0 2.0 36.0 88-89 26.49217554622519 33.5 25.0 35.0 2.0 36.0 90-91 25.98647580510656 33.0 23.0 35.0 2.0 35.5 92-93 25.47675506341506 33.0 19.5 35.0 2.0 35.0 94-95 24.916661227982395 32.0 15.0 35.0 2.0 35.0 96-97 24.24316357386821 32.0 2.0 34.5 2.0 35.0 98-99 23.43031232550888 31.5 2.0 34.0 2.0 35.0 100-101 21.097203791125516 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15464628186736107 1101 2 0.2076440882550088 1101 3 0.4264691370519671 1101 4 0.243863877331286 1101 5 -0.06591114101646411 1101 6 0.3085721035262381 1101 7 0.21157886737928067 1101 8 0.2543407139016338 1101 9 0.37182161334919783 1101 10-11 0.30902384632889124 1101 12-13 0.38366499651975516 1101 14-15 0.16136959478185275 1101 16-17 0.43768079838944374 1101 18-19 0.18987560875014253 1101 20-21 0.3414015726657311 1101 22-23 0.16383890046678573 1101 24-25 0.29279484666983535 1101 26-27 0.49813460514424435 1101 28-29 0.18864503063247895 1101 30-31 0.3489393224091586 1101 32-33 0.7136386507068835 1101 34-35 1.0358221600213398 1101 36-37 0.9518434650914074 1101 38-39 0.8386915604585425 1101 40-41 0.9393567245578609 1101 42-43 1.053296341101408 1101 44-45 1.2137228475483397 1101 46-47 1.8111213468824232 1101 48-49 1.8399808334864218 1101 50-51 2.0136522596012227 1101 52-53 1.8673555350233855 1101 54-55 2.3532096017213817 1101 56-57 2.3147992527134917 1101 58-59 1.7366256496595653 1101 60-61 2.455882429436514 1101 62-63 2.553980352621945 1101 64-65 2.7208450284652734 1101 66-67 2.595044697765413 1101 68-69 2.732754950906749 1101 70-71 2.5967309233251825 1101 72-73 2.762379114491676 1101 74-75 2.6204727876564924 1101 76-77 2.6111545512600642 1101 78-79 3.006259391005454 1101 80-81 2.4496187913136858 1101 82-83 2.4518630997897475 1101 84-85 2.6567128936096935 1101 86-87 2.713982561220842 1101 88-89 2.9842656788905195 1101 90-91 2.9422444793253035 1101 92-93 2.532533512453899 1101 94-95 2.228799212655648 1101 96-97 2.1251009937737457 1101 98-99 2.7922188427912786 1101 100-101 2.9891459602288073 1102 1 0.22682236626218 1102 2 0.5001360920707327 1102 3 0.4582153914709082 1102 4 0.1903315278559461 1102 5 -0.07188723663399088 1102 6 0.2478852733820247 1102 7 0.49094029490860436 1102 8 0.6515546749588168 1102 9 0.26373284167533484 1102 10-11 0.07758341088756282 1102 12-13 0.4304146177531294 1102 14-15 0.4435226726113868 1102 16-17 0.4792819992199284 1102 18-19 0.2594704687467697 1102 20-21 0.029438158715898055 1102 22-23 0.22687548651695266 1102 24-25 0.5727735235117635 1102 26-27 0.324652477317521 1102 28-29 0.2913959985916321 1102 30-31 0.8143102331324528 1102 32-33 0.6309763421944581 1102 34-35 0.8254453573727787 1102 36-37 1.1938613092078967 1102 38-39 1.3936197350839876 1102 40-41 1.4896204583550698 1102 42-43 1.1164241117091152 1102 44-45 1.1397090436091588 1102 46-47 1.2292446284042242 1102 48-49 0.983252815978986 1102 50-51 1.056663313975271 1102 52-53 1.227429324373027 1102 54-55 1.4668115320283164 1102 56-57 1.606877418921897 1102 58-59 1.6817956737884074 1102 60-61 1.3791962165416152 1102 62-63 1.37748913439626 1102 64-65 1.5184784384871612 1102 66-67 0.9338724852071927 1102 68-69 0.9361035284276511 1102 70-71 1.4382381339194232 1102 72-73 1.7005489702797938 1102 74-75 1.745122077819783 1102 76-77 1.8056652408958875 1102 78-79 2.122814298137488 1102 80-81 2.3022154469446825 1102 82-83 2.318544262463 1102 84-85 1.9794571279399769 1102 86-87 2.269275474076462 1102 88-89 2.62842846436833 1102 90-91 1.9875982754085761 1102 92-93 1.877842417678071 1102 94-95 1.9769474647178207 1102 96-97 2.0970301784435215 1102 98-99 2.3120690195490354 1102 100-101 2.5725517735950625 1103 1 0.2225537265353701 1103 2 0.24417094168327225 1103 3 0.08027659719745373 1103 4 0.10657862736997714 1103 5 -0.010966086071405812 1103 6 0.25517479660780396 1103 7 0.16166399352074734 1103 8 0.335171854591934 1103 9 0.4180605856445112 1103 10-11 0.1828436884585969 1103 12-13 0.14738191340568818 1103 14-15 0.29393421326197 1103 16-17 0.14823257056094263 1103 18-19 -0.06840484319228324 1103 20-21 -0.09730760548007567 1103 22-23 0.05342642561770106 1103 24-25 -0.029214130268719884 1103 26-27 0.07256193837439184 1103 28-29 -0.2294984456095861 1103 30-31 0.2345413963915064 1103 32-33 0.5832989200150394 1103 34-35 0.8208593059739755 1103 36-37 1.0009844649366357 1103 38-39 1.3185901829628008 1103 40-41 1.3424290043907092 1103 42-43 1.3803354991264811 1103 44-45 1.5008688001872486 1103 46-47 2.4014130973854506 1103 48-49 2.734927436179504 1103 50-51 2.2978961846902166 1103 52-53 2.1629947549583832 1103 54-55 2.3077445034393733 1103 56-57 2.0853262609165597 1103 58-59 2.5995532150519196 1103 60-61 2.190927314129283 1103 62-63 2.063382921883669 1103 64-65 2.4958218122448805 1103 66-67 2.788629282195064 1103 68-69 3.149776309829516 1103 70-71 2.750538383470669 1103 72-73 2.6164458224316256 1103 74-75 2.5976241574134953 1103 76-77 2.8578849335538266 1103 78-79 3.0080973417870673 1103 80-81 3.3522460935888745 1103 82-83 2.533410073942356 1103 84-85 2.6258933610296005 1103 86-87 2.2596217811558326 1103 88-89 2.3727697804227894 1103 90-91 2.8111502214704807 1103 92-93 2.6878113332246762 1103 94-95 2.4187078955220755 1103 96-97 2.840857068684752 1103 98-99 3.243967798061359 1103 100-101 3.960067644178661 1104 1 -0.06255005856841223 1104 2 -0.08855189103956107 1104 3 0.2261862431071009 1104 4 0.18716470795605744 1104 5 0.10075552565020018 1104 6 -0.27412923269622524 1104 7 0.04322687508362577 1104 8 0.3247933442134183 1104 9 0.4107345783185039 1104 10-11 0.31684857246347775 1104 12-13 0.18736970339347891 1104 14-15 0.6874018567296147 1104 16-17 0.19585161817998653 1104 18-19 0.2972850224975758 1104 20-21 0.008309000136534905 1104 22-23 0.6630112852025576 1104 24-25 0.07273947168487638 1104 26-27 -0.05259068677823109 1104 28-29 0.06018409407294456 1104 30-31 0.25957192142202956 1104 32-33 0.4025907393068593 1104 34-35 0.40480313991781003 1104 36-37 0.7128281767803415 1104 38-39 1.00754012191274 1104 40-41 0.7777159396776412 1104 42-43 0.7631193819103643 1104 44-45 0.3229078722263239 1104 46-47 0.8934765894489374 1104 48-49 1.0676502689023408 1104 50-51 1.4969193837134114 1104 52-53 1.1266699686335926 1104 54-55 1.309270754965631 1104 56-57 1.7400881656784577 1104 58-59 1.7985764140751215 1104 60-61 1.7388516120535797 1104 62-63 1.825287683788435 1104 64-65 1.7705470869701507 1104 66-67 1.2898502834160688 1104 68-69 1.3243794844326935 1104 70-71 1.3131146960469806 1104 72-73 1.3014275074133081 1104 74-75 1.1269281867175245 1104 76-77 1.315455141124037 1104 78-79 1.0261071097968326 1104 80-81 1.674895666238445 1104 82-83 1.7510535415858257 1104 84-85 2.0651485502847855 1104 86-87 2.0673140888481427 1104 88-89 1.8349187425717552 1104 90-91 1.6808083411286 1104 92-93 2.395992041405382 1104 94-95 2.0527127795269635 1104 96-97 1.426021903849584 1104 98-99 1.868509922603483 1104 100-101 2.5214229555339713 1105 1 0.35112515510680353 1105 2 0.20015706082728713 1105 3 0.5058983438718272 1105 4 0.4060580952705024 1105 5 0.665667806351955 1105 6 0.1577196202052633 1105 7 0.14893987553347188 1105 8 0.22672766720037885 1105 9 0.653919463608652 1105 10-11 0.6460883385453471 1105 12-13 0.44180065256126255 1105 14-15 0.37638071412951746 1105 16-17 0.6920729407171038 1105 18-19 0.4745679734647439 1105 20-21 0.45185375947077233 1105 22-23 0.38087003464025315 1105 24-25 0.744759264757306 1105 26-27 0.6002658134466898 1105 28-29 0.7587895821521258 1105 30-31 0.5287577063972932 1105 32-33 0.3761849813221545 1105 34-35 0.5916227530532083 1105 36-37 0.7188560670187627 1105 38-39 0.6942697665371185 1105 40-41 1.0779408609552021 1105 42-43 0.9965876680102284 1105 44-45 0.7037895636343308 1105 46-47 0.9578040748290562 1105 48-49 2.2639615599504737 1105 50-51 2.437225276650885 1105 52-53 1.8911324067802369 1105 54-55 1.7588434045382755 1105 56-57 1.7565524031953288 1105 58-59 1.988275019563197 1105 60-61 1.9921129705780984 1105 62-63 2.3421567796049025 1105 64-65 1.5992920262414039 1105 66-67 1.694933504288766 1105 68-69 2.2844784497948147 1105 70-71 2.4630022354081973 1105 72-73 2.198053688250017 1105 74-75 2.4549983621561218 1105 76-77 2.8808172643808945 1105 78-79 3.646529961798631 1105 80-81 3.1726914377184308 1105 82-83 2.931125516394644 1105 84-85 3.6036550730018355 1105 86-87 2.500917327714543 1105 88-89 2.8384724987570884 1105 90-91 2.60366034292797 1105 92-93 2.9883897022240973 1105 94-95 3.292883719697901 1105 96-97 3.2778437661977655 1105 98-99 3.335176589270148 1105 100-101 3.1828264643059008 1106 1 -0.15024470790689648 1106 2 -0.3371176327559873 1106 3 -0.2512754124367511 1106 4 -0.8869483935542988 1106 5 -0.6891988122493373 1106 6 -0.4241116703498946 1106 7 -0.18847901415650625 1106 8 -0.4920899247246453 1106 9 0.08477743044354469 1106 10-11 -0.4426643651316553 1106 12-13 -0.5339744015122747 1106 14-15 -0.183749062366509 1106 16-17 0.08327697820808311 1106 18-19 -0.29608811882076225 1106 20-21 0.3134254970338546 1106 22-23 0.16154775634176133 1106 24-25 -0.07542819018141245 1106 26-27 -0.07255322043938861 1106 28-29 -0.3064591561319432 1106 30-31 -0.34802672042318505 1106 32-33 0.49139767057954487 1106 34-35 0.44584048917433705 1106 36-37 0.35865419246938046 1106 38-39 0.9798501558666075 1106 40-41 0.8056986114959344 1106 42-43 0.973516290937404 1106 44-45 0.9580793978088025 1106 46-47 1.937616619890342 1106 48-49 1.653939930534463 1106 50-51 1.5059347214958763 1106 52-53 0.8149968624399406 1106 54-55 1.2054187470040318 1106 56-57 1.522666318119633 1106 58-59 1.6279289489071118 1106 60-61 1.2404907643776681 1106 62-63 1.485631905776497 1106 64-65 0.4207051480871158 1106 66-67 1.1866422281532145 1106 68-69 1.268472681950545 1106 70-71 0.9018630861652355 1106 72-73 0.594016197262274 1106 74-75 0.9534122049823672 1106 76-77 1.0266716263131208 1106 78-79 1.3121391903494626 1106 80-81 0.8472408248849739 1106 82-83 0.9868573116361716 1106 84-85 0.6174258011921729 1106 86-87 0.4698430667195872 1106 88-89 0.672935368259612 1106 90-91 1.0553546471817583 1106 92-93 1.1501777339472419 1106 94-95 0.7326097470951609 1106 96-97 0.8554212716051204 1106 98-99 0.25435467146193247 1106 100-101 0.9826101701458434 1107 1 0.0711251551068024 1107 2 0.2738412713536036 1107 3 0.6437930807139338 1107 4 0.06921599000734346 1107 5 0.1940888589835339 1107 6 0.0956143570473671 1107 7 0.039466191322944155 1107 8 0.2246224040424849 1107 9 -0.390291062707135 1107 10-11 0.12240412801903489 1107 12-13 0.4723269683507425 1107 14-15 0.4995386088663665 1107 16-17 0.3394413617697367 1107 18-19 0.32719955241211096 1107 20-21 0.3602748121023467 1107 22-23 0.10771213990341266 1107 24-25 0.45265400159941294 1107 26-27 -0.07447102865857858 1107 28-29 -0.19015778626893365 1107 30-31 -0.1628212409711267 1107 32-33 0.409342876058993 1107 34-35 0.7089911741058401 1107 36-37 0.7704350143871821 1107 38-39 0.4884802928529126 1107 40-41 -0.13416440220269976 1107 42-43 0.26132451011549307 1107 44-45 -0.4972630679446155 1107 46-47 -0.9543011883288415 1107 48-49 -1.1655121242600615 1107 50-51 -1.8680378812438505 1107 52-53 -1.8862360142723915 1107 54-55 -1.601156595461724 1107 56-57 -1.8955528599625637 1107 58-59 -1.8933039278052206 1107 60-61 -1.458413345211376 1107 62-63 -2.1457379572372055 1107 64-65 -2.331760605337543 1107 66-67 -2.8766454430796564 1107 68-69 -2.5160478659946612 1107 70-71 -2.555945133012848 1107 72-73 -2.6303673643815664 1107 74-75 -2.419738479949139 1107 76-77 -3.0549722093033154 1107 78-79 -3.976627932938211 1107 80-81 -3.6646769833342 1107 82-83 -3.188348167815885 1107 84-85 -3.034765979629743 1107 86-87 -2.799608988074933 1107 88-89 -3.6067906591376477 1107 90-91 -2.8542343939141297 1107 92-93 -1.4284524030390635 1107 94-95 -1.7371162803021 1107 96-97 -2.323208865381183 1107 98-99 -2.7948234107298475 1107 100-101 -1.6919103777993634 1108 1 -0.10749553454837013 1108 2 -0.11581390106019285 1108 3 0.06379308071393552 1108 4 0.15887116242113564 1108 5 -0.014187003085432082 1108 6 -0.1671442636422924 1108 7 -0.29570622247015876 1108 8 -0.7015844925092409 1108 9 -0.7992565799485192 1108 10-11 -0.015526906463726675 1108 12-13 -0.3666385488906414 1108 14-15 -0.8190820807888102 1108 16-17 -0.1467655347819985 1108 18-19 -0.6641797579327147 1108 20-21 -0.24007001548385887 1108 22-23 -1.1978051014759004 1108 24-25 -0.27665634322817567 1108 26-27 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0.15903117595203042 2314 4 0.39778741857877264 2314 5 0.18408885898353589 2314 6 0.041804833237840455 2314 7 0.32375190560865974 2314 8 0.8370033564234305 2314 9 0.3530422706261973 2314 10-11 0.6759755565904655 2314 12-13 0.3451841112078782 2314 14-15 0.5466814660092254 2314 16-17 0.25491755224592083 2314 18-19 0.9700566952692569 2314 20-21 0.6833700501975883 2314 22-23 0.9165216637129276 2314 24-25 1.1086063825517911 2314 26-27 1.1174337332461874 2314 28-29 0.8881755470643995 2314 30-31 0.3947978066479223 2314 32-33 0.27148573320185676 2314 34-35 0.3199435550582237 2314 36-37 0.40162549057765773 2314 38-39 0.15467076904339194 2314 40-41 -0.08797392601222498 2314 42-43 -0.3289135851226064 2314 44-45 -0.33750116318271495 2314 46-47 -2.27715833118598 2314 48-49 -2.9147978385457733 2314 50-51 -2.4466093098152797 2314 52-53 -2.005045538081916 2314 54-55 -2.017347071652196 2314 56-57 -1.642695717105422 2314 58-59 -2.4659229754242666 2314 60-61 -2.0219847737828047 2314 62-63 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0.015511240723796504 2315 20-21 0.12546961729714923 2315 22-23 -0.16410604191477063 2315 24-25 0.08966698861240019 2315 26-27 -0.1347307689183097 2315 28-29 -0.39807986419101127 2315 30-31 -0.26645760460748846 2315 32-33 -0.428189591473469 2315 34-35 0.3091210442357166 2315 36-37 0.07640904036120588 2315 38-39 0.19796081233343443 2315 40-41 0.08518624714795209 2315 42-43 0.4302855490765225 2315 44-45 0.4996200489384961 2315 46-47 0.7975169934893458 2315 48-49 0.22643592768799436 2315 50-51 -0.5732326864386508 2315 52-53 -0.38221004024641303 2315 54-55 -0.7879098422149653 2315 56-57 -0.9154229898326918 2315 58-59 -1.238109122610414 2315 60-61 -1.4846471114451418 2315 62-63 -0.9975561390553906 2315 64-65 -1.5840982676752056 2315 66-67 -2.066515572949786 2315 68-69 -1.5779959179427117 2315 70-71 -1.6328282498959652 2315 72-73 -2.206731000745201 2315 74-75 -1.9098683500790088 2315 76-77 -2.268738443069548 2315 78-79 -1.6140305303408091 2315 80-81 -1.9876639963212135 2315 82-83 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-0.6744608836176837 2316 40-41 -0.9955369512223129 2316 42-43 -0.5106362741982409 2316 44-45 -0.852557185591678 2316 46-47 -1.6980266785249185 2316 48-49 -1.4627670262208454 2316 50-51 -1.8464692537928684 2316 52-53 -1.674863465252784 2316 54-55 -2.497038948402899 2316 56-57 -1.6286901148645256 2316 58-59 -1.8815392219228642 2316 60-61 -2.336060404034903 2316 62-63 -1.717698741550933 2316 64-65 -1.500191977886562 2316 66-67 -2.191547403863975 2316 68-69 -2.94761649344564 2316 70-71 -1.800258858503046 2316 72-73 -2.4870340310482284 2316 74-75 -1.902483577988356 2316 76-77 -1.6428153465582191 2316 78-79 -1.898980874114681 2316 80-81 -1.9991867872557698 2316 82-83 -1.7524658148747072 2316 84-85 -1.7343738227669974 2316 86-87 -1.3086285959180692 2316 88-89 -1.6403200709023515 2316 90-91 -1.5003128252866809 2316 92-93 -1.019040638333177 2316 94-95 -0.6982927508903352 2316 96-97 -0.7941892575380471 2316 98-99 -1.4256077244553396 2316 100-101 -1.7334790052503415 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 599.0 3 83.0 4 91.0 5 93.0 6 80.0 7 110.0 8 130.0 9 197.0 10 241.0 11 343.0 12 559.0 13 852.0 14 1025.0 15 1420.0 16 2052.0 17 2978.0 18 2530.0 19 1304.0 20 1237.0 21 1143.0 22 1187.0 23 1370.0 24 1409.0 25 1553.0 26 1656.0 27 1779.0 28 2056.0 29 2425.0 30 2921.0 31 3529.0 32 4519.0 33 5947.0 34 8407.0 35 12745.0 36 17765.0 37 25862.0 38 22606.0 39 3098.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8504656590111 20.76962783630323 15.860760321926323 19.519146182759346 2 14.46669331685867 21.811970781698587 42.1012465021623 21.620089399280445 3 17.602337481102452 29.454733108500992 31.60323874869171 21.33969066170485 4 10.783544119784851 18.63861026311964 40.071231283616804 30.506614333478705 5 10.839572581231373 39.81754743039907 34.62310096678055 14.719779021589009 6 26.17634452346877 39.76215210931995 18.795359278174516 15.266144089036768 7 24.562142685912292 36.86573344278253 22.13513101213869 16.43699285916649 8 25.446370200339953 34.19144886900179 20.732788052242384 19.629392878415878 9 27.32926023058314 16.648625872678004 22.08847011645393 33.933643780284925 10-11 23.009746475394056 28.71014498018374 27.382764395395213 20.897344149026985 12-13 25.220401535818198 27.594962458180923 28.480201094833507 18.704434911167375 14-15 22.41131917830612 27.495867918702437 29.074232159398445 21.018580743593006 16-17 18.30375627438019 31.282552319795442 29.605631142718085 20.80806026310628 18-19 17.995808254904595 27.86529750856329 35.66942605056973 18.469468185962377 20-21 19.364117762426723 26.70320410846463 35.906208459543684 18.026469669564964 22-23 18.841490435957603 26.865405990512237 36.030613653369755 18.262489920160405 24-25 18.304983782095288 27.499246312379 36.04007075583064 18.155699149695074 26-27 18.045541555125332 27.41319753440679 36.22329818494855 18.317962725519326 28-29 17.652036843328684 26.98781090190609 36.95483205485821 18.40532019990702 30-31 17.75285469390127 27.367289420925704 36.49286689717291 18.386988988000116 32-33 17.514337705168114 27.8988678751866 36.01224743301504 18.57454698663025 34-35 18.062224417599502 27.71550816123432 35.88945468281238 18.332812738353805 36-37 18.192813531742715 27.36930804909143 35.66501890506786 18.772859514097988 38-39 17.769747593971307 27.991283820591974 35.7011076811331 18.537860904303614 40-41 17.403272782736906 28.053979851976667 36.11209970874703 18.430647656539392 42-43 17.71982711655105 28.05179239458105 35.15381542149421 19.074565067373698 44-45 17.623921317953336 28.42672225934858 34.806926809425576 19.142429613272512 46-47 18.11561831501033 28.39066307833673 34.118916091916454 19.374802514736487 48-49 18.12928579730358 28.7489830311483 33.42340771734077 19.698323454207344 50-51 18.563526681460445 28.87038254382747 32.808408482572524 19.757682292139563 52-53 18.275035414623517 29.5383386001235 32.601067887109075 19.58555809814391 54-55 18.26996756960913 29.098377027807338 32.60870354700591 20.022951855577627 56-57 18.50239142355979 29.346053697173506 32.09737106831446 20.054183810952246 58-59 18.45343153276825 28.485462561465436 32.789314274508094 20.271791631258214 60-61 18.69623475327448 28.914218899221822 32.76588945738179 19.6236568901219 62-63 18.487126411736934 28.86007916621273 33.240367505538 19.41242691651233 64-65 18.51729459827508 28.042850658193373 33.65592374035406 19.783931003177486 66-67 18.468795619112637 28.651163128499736 32.76913914691805 20.11090210546957 68-69 18.42100484811069 28.285003540755 33.208287182466904 20.085704428667405 70-71 18.449923193410974 28.26555106457927 33.2360086720631 20.04851706994665 72-73 18.356126576946263 28.224160596134713 32.805566248084425 20.614146578834603 74-75 18.175478612704165 28.55695227856331 32.63392257206885 20.633646536663676 76-77 18.293644020091012 28.22729138142053 33.01942639651059 20.459638201977867 78-79 17.969558632862295 27.741157585464265 33.12922872427356 21.16005505739988 80-81 17.87371892182427 28.229115974345753 32.84281325130559 21.054351852524388 82-83 18.481548589501053 28.077080889908824 32.51064818681259 20.93072233377754 84-85 17.929827491675958 27.772351465285922 32.245367764799006 22.052453278239113 86-87 18.04079913434375 27.860768778277254 32.450489836527495 21.647942250851496 88-89 18.308815786129035 27.326867140434523 32.49570594921218 21.86861112422426 90-91 17.879851984733655 27.52844282565356 32.84370154370191 21.74800364591088 92-93 18.080505474154364 27.595184922926087 32.71855765564573 21.605751947273816 94-95 17.629393458517473 27.38387906209942 32.66383664813476 22.322890831248344 96-97 17.994429171675208 27.681642050674927 32.29700431793669 22.02692445971318 98-99 18.17739393191013 27.92490793268009 31.647914230302675 22.249783905107105 100-101 18.44696859657787 27.36869244400546 31.96990384336054 22.214435116056126 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 286.0 1 196.0 2 148.5 3 184.5 4 144.0 5 125.5 6 235.5 7 604.5 8 1064.0 9 1207.5 10 894.5 11 604.0 12 580.0 13 596.0 14 589.0 15 551.5 16 570.5 17 591.5 18 592.0 19 587.0 20 631.0 21 713.0 22 768.5 23 866.5 24 954.0 25 1068.0 26 1315.0 27 1576.5 28 1716.5 29 1937.0 30 2274.0 31 2579.0 32 3138.0 33 3858.5 34 4407.0 35 4750.0 36 5511.5 37 6205.5 38 6021.0 39 5700.0 40 5355.0 41 5070.5 42 4787.0 43 4643.5 44 4739.0 45 4695.5 46 4285.0 47 3957.0 48 4322.5 49 4197.0 50 3425.5 51 2814.0 52 2466.5 53 2371.5 54 2247.0 55 2052.0 56 1813.0 57 1539.5 58 1320.0 59 1158.5 60 1018.5 61 855.5 62 686.0 63 527.5 64 365.5 65 250.5 66 196.0 67 154.0 68 102.5 69 67.5 70 51.5 71 40.0 72 28.0 73 22.0 74 21.0 75 15.0 76 11.0 77 11.0 78 8.0 79 3.0 80 1.0 81 1.0 82 2.0 83 1.5 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2567058977092262 2 0.2291498973901567 3 0.22987505529329014 4 0.23277568690582376 5 0.24002726593715784 6 0.1812894757833518 7 0.17548821255828456 8 0.17041210723635072 9 0.1812894757833518 10-11 0.189628791669386 12-13 0.18455268634745217 14-15 0.1874533179599858 16-17 0.17258758094575094 18-19 0.18092689683178512 20-21 0.1696869493332173 22-23 0.1812894757833518 24-25 0.1773011073161181 26-27 0.1787514231223849 28-29 0.17186242304261753 30-31 0.1689617914300839 32-33 0.17367531780045103 34-35 0.17258758094575094 36-37 0.17367531780045103 38-39 0.16388568610815005 40-41 0.1595347386893496 42-43 0.1711372651394841 44-45 0.1689617914300839 46-47 0.1685992124785172 48-49 0.17186242304261753 50-51 0.16714889667225039 52-53 0.17838884417081818 54-55 0.1606224755440497 56-57 0.1606224755440497 58-59 0.16098505449561643 60-61 0.150470264900182 62-63 0.15663410707681596 64-65 0.15482121231898247 66-67 0.15373347546428234 68-69 0.1584470018346495 70-71 0.1577218439315161 72-73 0.15518379127054915 74-75 0.17222500199418422 76-77 0.16497342296285017 78-79 0.16424826505971676 80-81 0.16243537030188324 82-83 0.14611931748138157 84-85 0.14321868586884795 86-87 0.14648189643294826 88-89 0.15264573860958225 90-91 0.15409605441584906 92-93 0.15119542280331544 94-95 0.15264573860958225 96-97 0.1591721597377829 98-99 0.16678631772068367 100-101 0.15155800175488213 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 137901.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.47964119186953 #Duplication Level Percentage of deduplicated Percentage of total 1 84.01942849933766 48.29406603287866 2 7.977039046237305 9.170346843025069 3 2.600138774995269 4.483651315073858 4 1.314577682457579 3.0224581402600417 5 0.8187724720873022 2.3531373956679067 6 0.5891629344603545 2.031892444579807 7 0.4062322588784457 1.6345059136627 8 0.311612943922286 1.432912016591613 9 0.27124203620765786 1.4031805425631432 >10 1.576988582602662 16.320403767920464 >50 0.09083454235791333 3.3748848811828775 >100 0.020185453857314073 2.324856237445704 >500 0.0 0.0 >1k 0.0037847725982463887 4.153704469148157 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2485 1.802017389286517 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1886 1.3676478053096062 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1357 0.9840392745520337 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 493 0.3575028462447698 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 434 0.3147185299598988 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 372 0.2697587399656275 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 240 0.17403789675201775 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 199 0.14430642272354807 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 183 0.13270389627341353 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 177 0.1283529488546131 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 155 0.11239947498567814 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.6257895156670364E-4 0.0 22-23 0.0 0.0 0.0 7.251579031334073E-4 0.0 24-25 0.0 0.0 0.0 0.002175473709400222 0.0 26-27 0.0 0.0 0.0 0.002900631612533629 0.0 28-29 0.0 0.0 0.0 0.006526421128200666 0.0 30-31 0.0 0.0 0.0 0.01486573701423485 0.0 32-33 0.0 0.0 0.0 0.023205052900269033 0.0 34-35 0.0 0.0 0.0 0.03190694773786992 0.0 36-37 0.0 0.0 0.0 0.04205915838173763 0.0 38-39 0.0 0.0 0.0 0.054024263783438844 0.0 40-41 0.0 0.0 0.0 0.06671452708827347 0.0 42-43 0.0 0.0 0.0 0.1000717906324102 0.0 44-45 0.0 0.0 0.0 0.1588095807862162 0.0 46-47 0.0 0.0 0.0 0.21428416037592185 0.0 48-49 0.0 0.0 0.0 0.3553273725353696 0.0 50-51 0.0 0.0 0.0 0.4597501105865802 0.0 52-53 0.0 0.0 0.0 0.5765005329910589 0.0 54-55 0.0 0.0 0.0 0.7762815353043125 0.0 56-57 0.0 0.0 0.0 0.9912908535833678 0.0 58-59 0.0 0.0 0.0 1.2309555405689587 0.0 60-61 0.0 0.0 0.0 1.5188432281129214 0.0 62-63 0.0 0.0 0.0 1.9307329170926968 0.0 64-65 0.0 0.0 0.0 2.224421867861727 0.0 66-67 0.0 0.0 0.0 2.572860240317329 0.0 68-69 0.0 0.0 0.0 2.988738297764338 0.0 70-71 0.0 0.0 0.0 3.3614694599749093 0.0 72-73 0.0 0.0 0.0 3.9267300454674006 0.0 74-75 0.0 0.0 0.0 4.548552947404298 0.0 76-77 0.0 0.0 0.0 5.108737427574855 0.0 78-79 0.0 0.0 0.0 5.62069890718704 0.0 80-81 0.0 0.0 0.0 6.235632809044169 0.0 82-83 0.0 0.0 0.0 6.797992762924126 0.0 84-85 0.0 0.0 0.0 7.431055612359591 0.0 86-87 0.0 0.0 0.0 8.408205886831858 0.0 88-89 0.0 0.0 0.0 9.560481794910842 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATAGA 25 0.0049924157 57.05611 1 TTAGGCA 35 2.765818E-4 54.339157 4 AGGCTTA 65 4.3724867E-8 51.16696 6 TACCTGG 55 4.9223927E-5 43.208607 2 ACCCGGG 55 4.9329785E-5 43.192894 7 CGACCCG 45 9.5662393E-4 42.248413 5 GGTATCA 1095 0.0 37.776875 1 CATGGGT 90 5.606071E-7 36.980816 4 GACCCGG 65 1.3201185E-4 36.547832 6 ACTATAC 55 0.0025639553 34.566887 3 CTAGATC 70 2.03219E-4 33.96197 4 TATACAA 85 1.614945E-5 33.55021 5 CATGGGG 185 0.0 33.411236 4 GTATCAA 1665 0.0 33.12567 1 ATATAGG 115 1.01956175E-7 33.063976 2 CTATACA 60 0.003920034 31.697838 4 ATGTTAT 60 0.003920034 31.697838 1 CCCGACC 60 0.0039270273 31.68631 3 CCGGGGA 75 3.05987E-4 31.674788 9 ACAATCA 60 0.00393403 31.674788 8 >>END_MODULE