FastQCFastQC Report
Thu 26 May 2016
SRR937706_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937706_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137901
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22531.6337807557595667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18901.37054843692214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12570.9115234842386929No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10840.7860711669966136No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.316894003669299No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.24582852916222508No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.16533600191441686No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2080.15083284385174872No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG1980.14358126482041464No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1930.1399554753047476No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA1770.1283529488546131No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.12472715933894606No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.12327684353267923No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC1640.1189258961138788No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG1580.11457494869507835No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA1490.10804852756687769No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG1420.10297242224494382No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT1410.10224726434181043No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA1410.10224726434181043No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.1000717906324102No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAATC156.606578E-494.996017
GGTAAGG405.353519E-447.532473
ACATAGA459.597573E-442.2204486
CGTAGCT704.28384E-640.7125788
AGCTTGA250.001614019537.99840572-73
GTATAGG651.316821E-436.5634421
GGTATCA13200.035.6493571
TCGTAGC801.0710612E-535.62357
TACTATA851.6142754E-533.5523342
CTATACA600.00392584431.6883144
GTTATAG902.3865226E-531.6883141
GTGTGCT753.0652765E-431.6653399
GTAGCTT902.3983977E-531.6653379
TTGAACG300.003946603731.66533774-75
ACGCTTT300.003946603731.66533778-79
GGTGTGC1252.1314918E-730.3987268
TATTCGT1102.6960952E-630.2479364
ACGCATT650.00580704729.2507535
TACGCAT650.00580704729.2507534
CCTATGC650.00580704729.2507533
Produced by FastQC (version 0.11.5)