##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937706_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 137901 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.393884018244975 33.0 31.0 34.0 31.0 34.0 2 32.589966715252245 34.0 31.0 34.0 31.0 34.0 3 32.626507421991136 34.0 31.0 34.0 31.0 34.0 4 36.223660452063434 37.0 35.0 37.0 35.0 37.0 5 36.167206909304504 37.0 35.0 37.0 35.0 37.0 6 36.247605166024904 37.0 37.0 37.0 35.0 37.0 7 36.20036838021479 37.0 36.0 37.0 35.0 37.0 8 36.20379112551758 37.0 36.0 37.0 35.0 37.0 9 37.88198780284407 39.0 38.0 39.0 35.0 39.0 10-11 37.82593672272137 39.0 38.0 39.0 35.0 39.0 12-13 37.744951088099434 39.0 37.0 39.0 35.0 39.0 14-15 38.87674491120441 40.0 38.0 41.0 35.5 41.0 16-17 38.85248475355509 40.0 38.0 41.0 35.5 41.0 18-19 38.9653555811778 40.0 38.0 41.0 35.5 41.0 20-21 38.92832539285429 40.0 38.0 41.0 35.0 41.0 22-23 38.77578117635115 40.0 38.0 41.0 35.0 41.0 24-25 38.79607109448082 40.0 38.0 41.0 35.0 41.0 26-27 38.635644411570624 40.0 38.0 41.0 35.0 41.0 28-29 38.47208142072937 40.0 38.0 41.0 34.0 41.0 30-31 38.354210629364545 40.0 37.5 41.0 35.0 41.0 32-33 38.10928854758124 40.0 37.0 41.0 34.0 41.0 34-35 37.91523266691322 40.0 36.0 41.0 34.0 41.0 36-37 37.682892074749276 40.0 36.0 41.0 33.0 41.0 38-39 37.46562388960196 40.0 35.0 41.0 33.0 41.0 40-41 37.21199628719154 40.0 35.0 41.0 33.0 41.0 42-43 36.878684708595294 40.0 35.0 41.0 33.0 41.0 44-45 36.327673475899374 39.5 35.0 41.0 31.0 41.0 46-47 35.64629698116765 39.0 35.0 41.0 28.5 41.0 48-49 35.024608233442834 39.0 35.0 41.0 24.0 41.0 50-51 34.64408524956309 39.0 35.0 41.0 21.0 41.0 52-53 34.37952589176294 39.0 35.0 40.5 19.5 41.0 54-55 34.129915664135865 38.5 35.0 40.0 16.5 41.0 56-57 33.84424333398597 38.0 34.0 40.0 10.0 41.0 58-59 33.57471301875984 38.0 34.0 40.0 6.5 41.0 60-61 33.18879486008078 38.0 34.0 40.0 2.0 41.0 62-63 32.82752119273972 37.0 33.0 40.0 2.0 41.0 64-65 32.43169012552484 37.0 33.0 40.0 2.0 41.0 66-67 31.98205596768696 36.0 32.0 39.0 2.0 41.0 68-69 31.500427843162846 36.0 31.5 39.0 2.0 40.0 70-71 31.039753156249773 35.0 31.0 38.5 2.0 40.0 72-73 30.531475478785502 35.0 31.0 38.0 2.0 40.0 74-75 30.007954982197376 35.0 30.0 37.0 2.0 39.0 76-77 28.768011109419078 33.5 28.5 36.0 2.0 38.5 78-79 29.257735621931676 34.0 29.0 36.0 2.0 39.0 80-81 29.018060057577536 34.0 29.0 36.0 2.0 37.5 82-83 28.654578284421433 34.0 29.0 35.5 2.0 37.0 84-85 28.252775541874243 34.0 29.0 35.0 2.0 37.0 86-87 27.81536029470417 34.0 28.0 35.0 2.0 36.0 88-89 27.45658117054989 34.0 27.0 35.0 2.0 36.0 90-91 26.956874859500658 33.5 26.0 35.0 2.0 35.5 92-93 26.499108055779146 33.0 25.0 35.0 2.0 35.0 94-95 26.033429779334448 33.0 24.0 35.0 2.0 35.0 96-97 25.491479394638183 33.0 21.5 35.0 2.0 35.0 98-99 24.68291382948637 32.0 10.0 34.5 2.0 35.0 100-101 22.28583186488858 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06945467041446562 1101 2 -0.04287517483867731 1101 3 0.0034306448173282433 1101 4 -0.07615586644447347 1101 5 -0.023811486211627653 1101 6 -0.03406073950053212 1101 7 -0.1261261496234738 1101 8 0.02372820409277665 1101 9 0.15671712608823185 1101 10-11 0.11702513075935883 1101 12-13 0.02653925100387511 1101 14-15 -0.24048949348752302 1101 16-17 -0.44689737266626395 1101 18-19 -0.05185688628970553 1101 20-21 0.0943543330299974 1101 22-23 -0.0404428046008789 1101 24-25 -0.1596186026849793 1101 26-27 -0.23016780356419986 1101 28-29 0.07363462596457993 1101 30-31 0.03161217360408841 1101 32-33 0.03242821357395087 1101 34-35 0.2230000504012679 1101 36-37 0.034859844355189296 1101 38-39 -0.16550740610721704 1101 40-41 -0.23106115482064382 1101 42-43 -0.01133386882522558 1101 44-45 -0.17210213690937337 1101 46-47 0.43279109901807544 1101 48-49 0.6613894917121357 1101 50-51 0.4737123248054189 1101 52-53 0.8247316358312631 1101 54-55 0.5317760233909681 1101 56-57 0.6449117484770639 1101 58-59 0.5461749693848645 1101 60-61 0.757983783003823 1101 62-63 0.7724408813908852 1101 64-65 0.6821147478552056 1101 66-67 0.6803603694617166 1101 68-69 0.867583768594038 1101 70-71 1.0328785123656417 1101 72-73 0.5246988172070921 1101 74-75 0.666421213101966 1101 76-77 0.7077648596530146 1101 78-79 0.7612784624616395 1101 80-81 0.9023502049812997 1101 82-83 0.9747949210378728 1101 84-85 0.7618531331300815 1101 86-87 0.7567970809149536 1101 88-89 0.48420050781938073 1101 90-91 0.851136484690155 1101 92-93 0.6639392795741905 1101 94-95 0.328435285199447 1101 96-97 0.28937057718262693 1101 98-99 -0.034868432359704826 1101 100-101 -0.1517788412471468 1102 1 0.15363905961821445 1102 2 0.14998717856461496 1102 3 0.09510619613319449 1102 4 5.513890406305677E-4 1102 5 0.11460667214088005 1102 6 0.010212870285670306 1102 7 0.011646702629484196 1102 8 0.18659785226066816 1102 9 0.20536759463657006 1102 10-11 0.049681827914398013 1102 12-13 0.22906015331014373 1102 14-15 -0.10183986664279843 1102 16-17 -0.020679708113199524 1102 18-19 0.009910130151567387 1102 20-21 0.17700261314895727 1102 22-23 0.5498790067452859 1102 24-25 0.2859442164609689 1102 26-27 0.17446016344101878 1102 28-29 0.37721607481706343 1102 30-31 0.25113548879402714 1102 32-33 0.16497548925993755 1102 34-35 -0.11632574500863768 1102 36-37 0.0628044041441953 1102 38-39 0.35677669691730074 1102 40-41 0.5061168935492617 1102 42-43 0.09295320821870945 1102 44-45 -0.12273622029414355 1102 46-47 -0.09862548815435446 1102 48-49 -0.13733111880355153 1102 50-51 -1.0607624521431234 1102 52-53 -0.5181924425032847 1102 54-55 -0.4682800901829012 1102 56-57 -0.529355969383893 1102 58-59 0.12363975811726391 1102 60-61 -0.3414143989163847 1102 62-63 0.001671844860950955 1102 64-65 0.10619308240433156 1102 66-67 -0.13113309330692857 1102 68-69 0.09730572583549346 1102 70-71 0.3229927034884774 1102 72-73 0.5331509242717907 1102 74-75 0.6696267010094914 1102 76-77 0.21895951764078347 1102 78-79 0.49562277535092747 1102 80-81 0.6519433815707139 1102 82-83 0.39091214229368987 1102 84-85 0.6119176637400336 1102 86-87 0.7745149418655188 1102 88-89 1.1754636243672714 1102 90-91 1.2561642609092267 1102 92-93 0.8946993379323054 1102 94-95 0.9615647392143742 1102 96-97 1.453124014139025 1102 98-99 1.6673859304706653 1102 100-101 1.9057445861699485 1103 1 0.08914976405321795 1103 2 0.18534027168663414 1103 3 0.056557095831109905 1103 4 0.03953829854546598 1103 5 0.13216282117113565 1103 6 0.11320748523247914 1103 7 0.1529715132770093 1103 8 -0.03624703193879952 1103 9 0.17529010240768628 1103 10-11 -0.0022095078414849922 1103 12-13 -0.12964787173254422 1103 14-15 0.13899897578967568 1103 16-17 0.011564939669291618 1103 18-19 0.011639476502431023 1103 20-21 0.2793295690615736 1103 22-23 0.4457241974111952 1103 24-25 0.36856975824985483 1103 26-27 0.4689306332102987 1103 28-29 -0.20695274441151668 1103 30-31 0.10499477691625714 1103 32-33 0.0684713957438916 1103 34-35 0.25331656487271914 1103 36-37 0.5223617790402173 1103 38-39 0.305607746345963 1103 40-41 -0.08267081276832755 1103 42-43 0.17172602902339662 1103 44-45 0.4584117154354672 1103 46-47 0.2107086040482642 1103 48-49 -0.155434529337235 1103 50-51 0.148433730160626 1103 52-53 -0.2669337410453849 1103 54-55 0.3986696931015459 1103 56-57 0.38607023787498207 1103 58-59 0.5137496482271473 1103 60-61 0.5056041204128903 1103 62-63 0.3182735698292092 1103 64-65 0.4518872288952913 1103 66-67 0.5088892349624103 1103 68-69 0.4874375064055201 1103 70-71 0.7683103340650703 1103 72-73 0.8664222346347366 1103 74-75 0.6203950561603158 1103 76-77 0.8751151150314378 1103 78-79 0.8279090150076875 1103 80-81 0.9455710686246981 1103 82-83 0.7735683307267713 1103 84-85 0.5440462920838023 1103 86-87 0.9224835071647632 1103 88-89 0.8035009257113472 1103 90-91 0.9093318104911141 1103 92-93 0.6748315563115348 1103 94-95 0.19737972104529433 1103 96-97 0.17197388936340374 1103 98-99 -0.33979414045442624 1103 100-101 -0.7931849703293317 1104 1 0.004900679804137553 1104 2 0.2213598077061647 1104 3 0.09013462940863803 1104 4 0.08166284067000618 1104 5 -0.07662838762007596 1104 6 0.21271908474408008 1104 7 -0.0924497321442388 1104 8 0.24336224767048265 1104 9 -0.49381856670098045 1104 10-11 0.03716778153580691 1104 12-13 0.34318485110018315 1104 14-15 0.06818090497160512 1104 16-17 -0.03605410794975228 1104 18-19 -0.0072860424230896115 1104 20-21 -0.040878001145998155 1104 22-23 0.05073030241729981 1104 24-25 -0.14455600487590203 1104 26-27 0.30836897264862984 1104 28-29 0.46581892836015015 1104 30-31 0.3125649844864711 1104 32-33 0.27329435056685014 1104 34-35 0.8369551485112936 1104 36-37 0.8541688608473024 1104 38-39 0.727158417896625 1104 40-41 1.3752046451071323 1104 42-43 1.4479775552749246 1104 44-45 1.7581675151912677 1104 46-47 1.2510016443413008 1104 48-49 0.9159940420913344 1104 50-51 0.45582078754768673 1104 52-53 1.1237866496750044 1104 54-55 0.70391999835185 1104 56-57 1.2990739008786463 1104 58-59 1.4783406128181156 1104 60-61 1.4063977712065423 1104 62-63 0.8280415795972189 1104 64-65 0.8450496220576831 1104 66-67 0.85534833142151 1104 68-69 0.906240925208941 1104 70-71 1.2866253523800886 1104 72-73 1.8252134434259446 1104 74-75 1.92564536141062 1104 76-77 1.6043580942744207 1104 78-79 1.4582508953495683 1104 80-81 1.2444111174647468 1104 82-83 2.1023229094813516 1104 84-85 2.247648245685756 1104 86-87 2.1053284400096928 1104 88-89 1.6032567254671477 1104 90-91 0.905668806828114 1104 92-93 0.4880183694983522 1104 94-95 0.21172648539205596 1104 96-97 0.026369493759009544 1104 98-99 0.3280935274332428 1104 100-101 0.5148125876682244 1105 1 0.3347252412076429 1105 2 0.5099787910619895 1105 3 0.4050244179826379 1105 4 0.09893679478606998 1105 5 0.3654768755378157 1105 6 0.2004833672452051 1105 7 0.3088997955210786 1105 8 0.4232093012017444 1105 9 0.5570136420259644 1105 10-11 0.5649680514413404 1105 12-13 0.5241115267636971 1105 14-15 0.3838804101447906 1105 16-17 0.5098298326710307 1105 18-19 0.5784989328355721 1105 20-21 0.5014521832894516 1105 22-23 0.487077580869844 1105 24-25 0.6419937027264311 1105 26-27 0.6300063995492167 1105 28-29 0.5124226170690989 1105 30-31 0.8730688081481901 1105 32-33 0.5204378503419278 1105 34-35 0.7428480859831836 1105 36-37 0.6615462787510324 1105 38-39 1.0257639059758006 1105 40-41 0.8421412173068603 1105 42-43 0.6485623505965705 1105 44-45 0.1710779965228042 1105 46-47 -0.11485530572618075 1105 48-49 -0.3722650672204111 1105 50-51 -0.054660543986280175 1105 52-53 0.3451541710425303 1105 54-55 0.45772116794248774 1105 56-57 -0.22656712476237573 1105 58-59 -0.23425497872483447 1105 60-61 0.2428800024256148 1105 62-63 0.03065066641683245 1105 64-65 -0.5351573055176644 1105 66-67 -0.44226750303643314 1105 68-69 -0.5012264612058139 1105 70-71 0.18797488004540597 1105 72-73 -0.046446475602397896 1105 74-75 -0.8841162220351748 1105 76-77 -0.7577651625856809 1105 78-79 -0.4921314708222724 1105 80-81 -0.30691591807281426 1105 82-83 0.05954288249079909 1105 84-85 -0.08640483573574542 1105 86-87 0.26461768877262415 1105 88-89 -0.1814036434037476 1105 90-91 -0.16828530923126905 1105 92-93 -0.11643507179719492 1105 94-95 -0.16326226854406656 1105 96-97 0.46442617392095187 1105 98-99 1.3859432800198377 1105 100-101 1.6933101135341708 1106 1 0.1647180314023089 1106 2 -0.082234619175928 1106 3 -0.06289194827547107 1106 4 0.12662244727344785 1106 5 0.12885106443471983 1106 6 0.2904617378291974 1106 7 0.22613123027233684 1106 8 -0.13785774998787304 1106 9 0.21382690806778015 1106 10-11 0.17801779909815707 1106 12-13 0.046966609676459825 1106 14-15 0.2802755074771639 1106 16-17 0.3778903950358412 1106 18-19 0.2776337561953355 1106 20-21 -0.23892272658798674 1106 22-23 0.02889445181432393 1106 24-25 0.3478336450479915 1106 26-27 0.35098693307481454 1106 28-29 0.1624586660957803 1106 30-31 -0.1943428717364526 1106 32-33 -0.37797599248431624 1106 34-35 -0.2230495347810546 1106 36-37 -0.2362186815950409 1106 38-39 -0.091539626828812 1106 40-41 -1.0601659204004221 1106 42-43 -0.9957058541619048 1106 44-45 -0.5575449306581675 1106 46-47 -1.0368452119987097 1106 48-49 -1.2452282972131954 1106 50-51 -0.674199116444818 1106 52-53 -0.30690062347802183 1106 54-55 -0.27248791641223136 1106 56-57 -0.37725205626922076 1106 58-59 -0.6777301048964404 1106 60-61 -0.3616621749356028 1106 62-63 -0.5624279204613245 1106 64-65 -0.6719273127274725 1106 66-67 -1.2343367171676505 1106 68-69 -1.4967563818979563 1106 70-71 -0.5865745071211386 1106 72-73 -0.42294251020946305 1106 74-75 -0.47647382837980246 1106 76-77 -0.4819901085121394 1106 78-79 -0.6591826604401554 1106 80-81 -0.2521934955782221 1106 82-83 -0.5843648320009116 1106 84-85 0.29600323924622884 1106 86-87 0.2733415532282848 1106 88-89 -0.21983190584066392 1106 90-91 -0.5333898514086286 1106 92-93 0.27779708393459757 1106 94-95 1.016550276517215 1106 96-97 1.467886880481874 1106 98-99 0.5129079519736948 1106 100-101 1.1026828604700079 1107 1 0.2715673464708033 1107 2 0.3005051068514675 1107 3 0.13765599693000752 1107 4 0.04525258425974954 1107 5 -0.2966283876200748 1107 6 -0.1489903169653246 1107 7 0.37626821657371323 1107 8 0.5158408801491134 1107 9 0.46122416834175084 1107 10-11 0.6107575251255497 1107 12-13 0.5846378425531711 1107 14-15 0.7649330417237437 1107 16-17 0.5703561484605046 1107 18-19 0.3495515644145186 1107 20-21 0.567241656973664 1107 22-23 0.3328670545540504 1107 24-25 0.9798884395685405 1107 26-27 0.27948008375974354 1107 28-29 0.49821209075332007 1107 30-31 0.306753018674506 1107 32-33 -0.014825307552804645 1107 34-35 -0.08030980875366112 1107 36-37 0.18049364717208505 1107 38-39 0.08681653755475338 1107 40-41 0.3216149015173855 1107 42-43 0.47908866638604053 1107 44-45 0.544235891259639 1107 46-47 0.863565746905401 1107 48-49 -0.6901598040625103 1107 50-51 -1.522555280828378 1107 52-53 -0.6348458289574737 1107 54-55 -0.27385777942593137 1107 56-57 -0.546567124762376 1107 58-59 0.3736397581172639 1107 60-61 -0.23659368178491036 1107 62-63 -0.3477703862147479 1107 64-65 -0.5504204634124008 1107 66-67 -0.7785832925101133 1107 68-69 -0.5733317243637082 1107 70-71 -0.7246566989019634 1107 72-73 -1.3117096334971343 1107 74-75 -1.4167478009825416 1107 76-77 -1.9782914783751515 1107 78-79 -2.690552523453853 1107 80-81 -3.5584948654412365 1107 82-83 -3.6320360648776244 1107 84-85 -2.7016679936304833 1107 86-87 -2.684329679648428 1107 88-89 -3.192982590772168 1107 90-91 -2.8140747829154797 1107 92-93 -2.162750861270883 1107 94-95 -2.485367531701961 1107 96-97 -2.249784352394837 1107 98-99 -2.0435304041906868 1107 100-101 -1.131426728571089 1108 1 -0.24222575697747573 1108 2 -0.2553569621140497 1108 3 -0.30096469272516657 1108 4 0.10111465322526669 1108 5 -0.45593873244766314 1108 6 -0.49933514455153016 1108 7 -0.24718005928836106 1108 8 -0.5551936026095063 1108 9 -0.017396521313422397 1108 10-11 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-0.4267989558965226 2316 56-57 -0.5549984973113951 2316 58-59 0.30011034635255385 2316 60-61 -0.0858093680594223 2316 62-63 -0.387770386214747 2316 64-65 -0.15218516929475712 2316 66-67 0.5892598447447881 2316 68-69 -0.03254741063821953 2316 70-71 -0.24995081654902052 2316 72-73 -0.48700375114419003 2316 74-75 -0.3543948598060709 2316 76-77 -0.3996640273947598 2316 78-79 -0.40172899404208806 2316 80-81 -0.6012399634804488 2316 82-83 -1.0387027315442872 2316 84-85 -1.2612758367677372 2316 86-87 -1.2349179149425424 2316 88-89 -1.7178845515564873 2316 90-91 -1.8409375280135158 2316 92-93 -1.2917704691140166 2316 94-95 -1.128896943466664 2316 96-97 -1.1335098425909145 2316 98-99 -1.838824521837747 2316 100-101 -0.7180933952377586 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 4.0 6 8.0 7 12.0 8 36.0 9 81.0 10 141.0 11 261.0 12 462.0 13 655.0 14 883.0 15 1276.0 16 2023.0 17 3059.0 18 2914.0 19 1398.0 20 1056.0 21 1088.0 22 999.0 23 1102.0 24 1224.0 25 1264.0 26 1402.0 27 1520.0 28 1896.0 29 2303.0 30 2691.0 31 3240.0 32 4211.0 33 5689.0 34 8026.0 35 12221.0 36 18185.0 37 28816.0 38 25131.0 39 2624.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.59307233721588 21.833886946809592 16.24287434182852 18.33016637414601 2 15.213087649835751 23.352259954605113 40.91485921059311 20.519793184966026 3 18.471222108614153 29.832996134908374 31.422542258576808 20.27323949790067 4 11.442266553542034 20.19854823387793 38.89964539778536 29.45953981479467 5 11.285514300013782 40.90609065258546 33.33792713267134 14.470467914729412 6 27.542222318909943 39.975054568132215 17.44657399148665 15.036149121471201 7 25.14122450163523 37.803931806150786 21.270331614709104 15.784512077504878 8 26.893205995605545 34.570452716078925 19.367517276887042 19.16882401142849 9 28.4290904344421 17.429170201811445 21.321817825831573 32.81992153791488 10-11 23.706499590285784 30.051993821654666 26.058911828050558 20.182594760008993 12-13 25.891681985794 28.24230689741442 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17.81314131152058 38-39 18.2432260941766 28.929916860344957 35.30444052052023 17.522416524958214 40-41 18.150009427257828 29.214709422906786 35.04619356335842 17.58908758647696 42-43 18.631830873554048 29.22362838597382 34.15056024948326 17.993980490988868 44-45 18.735904743257844 29.20355907498858 33.715854127236206 18.34468205451737 46-47 18.862082218403057 30.11544513817884 32.694106641721234 18.328366001696867 48-49 19.05026069426618 30.484187931922175 32.13029637203501 18.335255001776638 50-51 19.465776172761622 30.357647877825393 31.436320258736338 18.740255690676644 52-53 19.038002037701098 30.816784565683232 31.104673297051132 19.04054009956454 54-55 19.13220353732025 30.7006475660075 30.87214741009855 19.2950014865737 56-57 19.417915751154812 30.891364094531582 30.795643251317973 18.895076902995626 58-59 19.304065960362866 30.083900769392535 31.15495899232058 19.45707427792402 60-61 19.126402274095184 30.688682460605797 31.306879573027025 18.87803569227199 62-63 19.377306908579342 30.145901770110438 31.883380106018084 18.59341121529213 64-65 19.2884750654455 29.94068208352369 31.634288366291756 19.13655448473905 66-67 19.137279642642184 30.377952299113133 31.284037099078326 19.20073095916636 68-69 19.214146380374327 30.071210506087702 31.084981254668204 19.629661858869767 70-71 19.12676485304675 30.562867564412148 31.114712728696674 19.195654853844424 72-73 19.214508959325894 29.94068208352369 30.93487356871959 19.90993538843083 74-75 19.265270012545233 30.470772510714205 30.171644875671678 20.092312601068883 76-77 19.00928927273914 29.90950029368895 31.160035097642513 19.921175335929398 78-79 19.142355747964118 29.173465022008543 30.94212514775092 20.742054082276415 80-81 18.832713323326153 30.479111826600242 30.599488038520388 20.088686811553217 82-83 19.20782888987995 30.3391237821747 30.037817396002158 20.41522993194319 84-85 18.668170166170537 30.14238527053927 29.887853923662348 21.30159063962785 86-87 18.70871132189034 30.32827898274849 29.826469713780174 21.13653998158099 88-89 18.520170267075656 29.842060608697548 30.255400613483584 21.382368510743213 90-91 18.39087962056437 29.97229655737586 30.445865877626204 21.190957944433567 92-93 18.703838665114812 29.704241132128832 29.666532510995324 21.925387691761028 94-95 18.64308453165677 29.686876817426995 29.708631554521 21.96140709639524 96-97 19.36026569785571 29.917477030623417 28.88630249236771 21.83595477915316 98-99 19.397962994521333 30.206274905093238 28.351287359906017 22.044474740479412 100-101 19.033925042156987 30.427734764555492 28.08398759768635 22.45435259560117 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 558.0 1 346.0 2 168.5 3 192.5 4 166.0 5 180.0 6 284.0 7 545.0 8 785.0 9 827.0 10 768.5 11 702.0 12 702.0 13 704.0 14 690.0 15 700.5 16 701.0 17 671.5 18 655.0 19 654.0 20 683.5 21 726.0 22 790.0 23 908.0 24 971.0 25 1074.0 26 1283.0 27 1452.0 28 1598.0 29 1875.0 30 2233.5 31 2620.0 32 3074.5 33 3589.5 34 4062.0 35 4512.5 36 5310.5 37 5756.0 38 5863.0 39 5866.0 40 5468.5 41 5136.0 42 4680.0 43 4541.5 44 4726.0 45 4643.5 46 4201.0 47 3919.5 48 4413.0 49 4269.0 50 3481.5 51 2885.5 52 2569.0 53 2474.5 54 2233.0 55 2048.5 56 1864.5 57 1646.0 58 1501.0 59 1352.5 60 1137.0 61 939.5 62 746.5 63 569.0 64 394.0 65 264.5 66 193.5 67 173.5 68 134.5 69 73.5 70 47.0 71 33.5 72 32.0 73 30.5 74 23.5 75 21.0 76 17.5 77 13.5 78 10.0 79 8.0 80 3.5 81 1.0 82 2.0 83 3.5 84 3.0 85 1.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013778000159534738 2 0.0 3 0.0 4 0.0 5 0.024655368706535848 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.001087736854700111 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.6257895156670364E-4 28-29 0.001087736854700111 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.6257895156670364E-4 40-41 0.002175473709400222 42-43 0.011602526450134517 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.6257895156670364E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.001087736854700111 84-85 3.6257895156670364E-4 86-87 0.0 88-89 0.0 90-91 0.008701894837600888 92-93 0.001087736854700111 94-95 0.0 96-97 0.0 98-99 0.0018128947578335182 100-101 0.01704121072363507 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 137901.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.35783641888021 #Duplication Level Percentage of deduplicated Percentage of total 1 82.75747065101388 44.98517052088092 2 8.581910352187833 9.329881581714417 3 2.884204909284952 4.703374159723279 4 1.424759871931697 3.097874562185916 5 0.9031483457844184 2.454659502106584 6 0.5536286019210246 1.8056431788021843 7 0.4615795090715048 1.7563324413891124 8 0.35352187833511206 1.5373347546428235 9 0.2841515474919957 1.3901277003067418 >10 1.635538954108858 16.59813924482056 >50 0.11472785485592316 4.149353521729356 >100 0.040021344717182494 3.490184987781089 >500 0.0 0.0 >1k 0.005336179295624333 4.701923843917013 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2253 1.6337807557595667 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1890 1.37054843692214 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1257 0.9115234842386929 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1084 0.7860711669966136 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 437 0.316894003669299 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 339 0.24582852916222508 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 228 0.16533600191441686 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 208 0.15083284385174872 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 198 0.14358126482041464 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 193 0.1399554753047476 No Hit GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA 177 0.1283529488546131 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 172 0.12472715933894606 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 170 0.12327684353267923 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 164 0.1189258961138788 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 158 0.11457494869507835 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 149 0.10804852756687769 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 142 0.10297242224494382 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 141 0.10224726434181043 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 141 0.10224726434181043 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 138 0.1000717906324102 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.6257895156670364E-4 0.0 22-23 0.0 0.0 0.0 7.251579031334073E-4 0.0 24-25 0.0 0.0 0.0 0.0025380526609669256 0.0 26-27 0.0 0.0 0.0 0.0036257895156670363 0.0 28-29 0.0 0.0 0.0 0.0072515790313340735 0.0 30-31 0.0 0.0 0.0 0.015590894917368257 0.0 32-33 0.0 0.0 0.0 0.023930210803402442 0.0 34-35 0.0 0.0 0.0 0.03263210564100333 0.0 36-37 0.0 0.0 0.0 0.04278431628487103 0.0 38-39 0.0 0.0 0.0 0.055474579589705655 0.0 40-41 0.0 0.0 0.0 0.068527421846107 0.0 42-43 0.0 0.0 0.0 0.10188468539024373 0.0 44-45 0.0 0.0 0.0 0.16025989659248302 0.0 46-47 0.0 0.0 0.0 0.21464673932748857 0.0 48-49 0.0 0.0 0.0 0.3556899514869363 0.0 50-51 0.0 0.0 0.0 0.4597501105865802 0.0 52-53 0.0 0.0 0.0 0.5794011646035924 0.0 54-55 0.0 0.0 0.0 0.7831705353840799 0.0 56-57 0.0 0.0 0.0 1.0003553273725352 0.0 58-59 0.0 0.0 0.0 1.2421954880675266 0.0 60-61 0.0 0.0 0.0 1.5322586493208896 0.0 62-63 0.0 0.0 0.0 1.948861864671032 0.0 64-65 0.0 0.0 0.0 2.2472643418104292 0.0 66-67 0.0 0.0 0.0 2.594977556362898 0.0 68-69 0.0 0.0 0.0 3.0152065612287076 0.0 70-71 0.0 0.0 0.0 3.3933764077127795 0.0 72-73 0.0 0.0 0.0 3.961175045866237 0.0 74-75 0.0 0.0 0.0 4.592062421592302 0.0 76-77 0.0 0.0 0.0 5.15877332289106 0.0 78-79 0.0 0.0 0.0 5.673635434115779 0.0 80-81 0.0 0.0 0.0 6.29944670451991 0.0 82-83 0.0 0.0 0.0 6.873771763801567 0.0 84-85 0.0 0.0 0.0 7.5126358764621 0.0 86-87 0.0 0.0 0.0 8.5013886773845 0.0 88-89 0.0 0.0 0.0 9.67179353304182 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACAATC 15 6.606578E-4 94.99601 7 GGTAAGG 40 5.353519E-4 47.53247 3 ACATAGA 45 9.597573E-4 42.220448 6 CGTAGCT 70 4.28384E-6 40.712578 8 AGCTTGA 25 0.0016140195 37.998405 72-73 GTATAGG 65 1.316821E-4 36.563442 1 GGTATCA 1320 0.0 35.649357 1 TCGTAGC 80 1.0710612E-5 35.6235 7 TACTATA 85 1.6142754E-5 33.552334 2 CTATACA 60 0.003925844 31.688314 4 GTTATAG 90 2.3865226E-5 31.688314 1 GTGTGCT 75 3.0652765E-4 31.665339 9 GTAGCTT 90 2.3983977E-5 31.665337 9 TTGAACG 30 0.0039466037 31.665337 74-75 ACGCTTT 30 0.0039466037 31.665337 78-79 GGTGTGC 125 2.1314918E-7 30.398726 8 TATTCGT 110 2.6960952E-6 30.247936 4 ACGCATT 65 0.005807047 29.250753 5 TACGCAT 65 0.005807047 29.250753 4 CCTATGC 65 0.005807047 29.250753 3 >>END_MODULE