FastQCFastQC Report
Thu 26 May 2016
SRR937705_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937705_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116399
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22561.9381609807644398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15961.3711458002216514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12221.0498371979140715No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4770.40979733502865145No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.32474505794723324No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.28350759027139405No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.16838632634300982No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.14862670641500356No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.1451902507753503No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.14089468122578372No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1280.10996658046890438No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT1200.10309366918959785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8600.038.158921
GAGTACC500.001599808738.0483171
CCTGACC651.3143255E-436.5691453
ACATAAG550.002559553134.5744633
GTATCAA13100.032.6750831
TACTCCC600.003928591531.6795985
GACAGAT804.4696554E-429.6868277
AGGCCTG650.005811115329.2427045
ATTATAC856.341028E-427.964643
CAGTGCA908.900144E-426.388299
CATGGGG1658.241841E-825.9196724
GAGTACT10500.025.78513112-13
ATCAACG16700.025.3356293
TATCAAC16850.025.110092
CATGGGC950.00122008124.9994346
TCAACGC16750.024.9654144
CAACGCA17100.024.4544265
AACGCAG17250.024.2313356
GTACATG9450.024.1576611
ATGGGAC1000.001644951223.749467
Produced by FastQC (version 0.11.5)