##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937705_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 116399 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03359135387761 33.0 31.0 34.0 31.0 34.0 2 32.133480528183235 33.0 31.0 34.0 31.0 34.0 3 32.1069940463406 34.0 31.0 34.0 30.0 34.0 4 35.714516447735804 37.0 35.0 37.0 35.0 37.0 5 35.72071065902628 37.0 35.0 37.0 35.0 37.0 6 35.80566843357761 37.0 36.0 37.0 35.0 37.0 7 35.768846811398724 37.0 35.0 37.0 35.0 37.0 8 35.76766982534214 37.0 36.0 37.0 35.0 37.0 9 37.42085413104924 39.0 37.0 39.0 35.0 39.0 10-11 37.34843941958265 39.0 37.0 39.0 35.0 39.0 12-13 37.22829233928127 39.0 37.0 39.0 34.5 39.0 14-15 38.30188403680444 40.0 38.0 41.0 34.0 41.0 16-17 38.306957104442475 40.0 38.0 41.0 34.0 41.0 18-19 38.384917396197565 40.0 38.0 41.0 34.0 41.0 20-21 38.409367778073694 40.0 38.0 41.0 34.0 41.0 22-23 38.218614421086095 40.0 38.0 41.0 34.0 41.0 24-25 38.07010799061848 40.0 38.0 41.0 34.0 41.0 26-27 37.9578561671492 40.0 38.0 41.0 33.5 41.0 28-29 37.94806656414573 40.0 38.0 41.0 34.0 41.0 30-31 37.63357932628287 40.0 37.0 41.0 33.0 41.0 32-33 37.274680194847036 39.0 35.5 41.0 33.0 41.0 34-35 37.19553862146582 39.5 35.0 41.0 33.0 41.0 36-37 37.03792128798358 40.0 35.0 41.0 33.0 41.0 38-39 36.84483114116101 40.0 35.0 41.0 32.5 41.0 40-41 36.52248301102243 39.0 35.0 41.0 31.5 41.0 42-43 36.13567126865351 39.0 35.0 41.0 30.5 41.0 44-45 35.664760865643174 39.0 35.0 41.0 29.0 41.0 46-47 34.980721483861544 39.0 35.0 41.0 24.0 41.0 48-49 34.38026529437538 39.0 35.0 40.5 17.5 41.0 50-51 33.36788975850308 38.0 34.0 39.5 9.5 40.5 52-53 33.40035137758915 38.0 33.5 39.5 7.0 40.5 54-55 33.675955119889345 38.0 34.5 40.0 2.0 41.0 56-57 33.460261686096956 38.0 34.0 40.0 2.0 41.0 58-59 33.20675435355974 38.0 34.0 40.0 2.0 41.0 60-61 32.89177312519867 38.0 33.5 40.0 2.0 41.0 62-63 32.51939449651629 37.5 33.0 40.0 2.0 41.0 64-65 32.13169786682016 37.0 33.0 40.0 2.0 41.0 66-67 31.69760049484961 36.5 32.0 39.5 2.0 41.0 68-69 31.18158231599928 36.0 31.0 39.0 2.0 41.0 70-71 30.753846682531638 35.5 31.0 39.0 2.0 40.0 72-73 30.298666655211814 35.0 30.5 38.5 2.0 40.0 74-75 29.829074992053194 35.0 30.0 37.5 2.0 40.0 76-77 29.334457340698805 35.0 29.0 37.0 2.0 39.0 78-79 28.84194881399325 35.0 29.0 36.5 2.0 39.0 80-81 28.309057637952215 34.0 28.0 36.0 2.0 38.5 82-83 27.7953633622282 34.0 26.5 35.5 2.0 37.0 84-85 27.351897353070044 34.0 26.0 35.0 2.0 37.0 86-87 26.907099717351525 34.0 25.5 35.0 2.0 36.0 88-89 26.450265895755116 34.0 24.5 35.0 2.0 36.0 90-91 25.97447143016693 33.0 22.5 35.0 2.0 35.5 92-93 25.4786982706037 33.0 19.5 35.0 2.0 35.0 94-95 24.90328954716106 32.5 14.0 35.0 2.0 35.0 96-97 24.260341583690582 32.0 2.0 35.0 2.0 35.0 98-99 23.41606457100147 31.5 2.0 34.0 2.0 35.0 100-101 21.26132526911743 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.02714323717364664 1101 2 0.07551832477311393 1101 3 0.0696928728922046 1101 4 0.11449396300982073 1101 5 0.20225983444265694 1101 6 0.27228806920268767 1101 7 0.1507331335818023 1101 8 0.005430502438663609 1101 9 0.10922388626629953 1101 10-11 -0.03913040343265095 1101 12-13 0.13347912702537457 1101 14-15 0.4019945163681271 1101 16-17 0.45027865313067394 1101 18-19 0.620097824761551 1101 20-21 0.749759568114186 1101 22-23 0.5862338938364715 1101 24-25 0.4582955991804596 1101 26-27 0.4200914684995922 1101 28-29 0.6410969401587252 1101 30-31 0.4458291224222606 1101 32-33 -0.04725562007816819 1101 34-35 0.6747481832845637 1101 36-37 0.7428035725792057 1101 38-39 0.527044402223872 1101 40-41 0.7895719855699141 1101 42-43 0.7987584176266509 1101 44-45 1.0309131565736465 1101 46-47 1.3207429040194896 1101 48-49 1.5926258848436134 1101 50-51 1.8902502565527826 1101 52-53 2.3353850656348314 1101 54-55 1.9784945116899237 1101 56-57 2.195842957608427 1101 58-59 2.5020050726031613 1101 60-61 2.472613137412349 1101 62-63 2.2544544007629135 1101 64-65 2.089189987551837 1101 66-67 2.1732173361326765 1101 68-69 2.0739476985971486 1101 70-71 2.4646123721150026 1101 72-73 2.9859320292369382 1101 74-75 2.8284982386849613 1101 76-77 2.9619471914858337 1101 78-79 2.841918202475277 1101 80-81 3.21746812737144 1101 82-83 2.962126620636525 1101 84-85 3.279511268566246 1101 86-87 3.5881303283911095 1101 88-89 3.268246365712365 1101 90-91 4.012213687233004 1101 92-93 3.8961040042302493 1101 94-95 4.359220221198591 1101 96-97 3.969336713214588 1101 98-99 4.114354830410189 1101 100-101 3.908254517818058 1102 1 0.4740533495332002 1102 2 0.015054841627048177 1102 3 0.40613950210568817 1102 4 0.20051924390869402 1102 5 0.2715014074763644 1102 6 0.33240042875324605 1102 7 -0.16689327091258122 1102 8 -0.3254683739658262 1102 9 -0.22448397890223504 1102 10-11 0.169928585331391 1102 12-13 0.27824738545233885 1102 14-15 0.561545078165878 1102 16-17 0.25989943964752626 1102 18-19 0.40380568993008836 1102 20-21 0.58269496137261 1102 22-23 0.5462058039488298 1102 24-25 0.49800767783214894 1102 26-27 0.354958041533294 1102 28-29 0.0746643558890554 1102 30-31 0.7304850212986622 1102 32-33 0.9312556158768928 1102 34-35 1.1864757113744488 1102 36-37 1.7196294152758327 1102 38-39 1.8105767055946629 1102 40-41 2.0547756372552968 1102 42-43 2.081553361446872 1102 44-45 2.356088718371403 1102 46-47 2.829731668064433 1102 48-49 2.850807064618891 1102 50-51 2.826907559923569 1102 52-53 2.718285346533712 1102 54-55 2.9611841184314898 1102 56-57 3.1837291935634866 1102 58-59 3.634624454625637 1102 60-61 3.781917912693242 1102 62-63 3.9226426030101074 1102 64-65 4.1900677965406 1102 66-67 3.950534751863014 1102 68-69 4.019242642417378 1102 70-71 4.212962091216124 1102 72-73 4.355559838225705 1102 74-75 4.289242620707437 1102 76-77 4.236666292609428 1102 78-79 4.278891517082016 1102 80-81 4.36925885770852 1102 82-83 4.327821845355629 1102 84-85 4.414377841599958 1102 86-87 4.3766485868180744 1102 88-89 5.205184567959552 1102 90-91 5.061300765884692 1102 92-93 5.457761307601032 1102 94-95 4.841804490861509 1102 96-97 4.845846544675265 1102 98-99 5.2801202798483935 1102 100-101 5.712678675121431 1103 1 0.1383976118282817 1103 2 0.41351795638114197 1103 3 0.17468458407289944 1103 4 0.3270561291545988 1103 5 0.07949321075505367 1103 6 0.1987938713761963 1103 7 0.202676401218568 1103 8 0.2737119539030246 1103 9 -0.14579545431207208 1103 10-11 0.37351465090516456 1103 12-13 0.20821664774742743 1103 14-15 0.4254795043953905 1103 16-17 0.5528297675163785 1103 18-19 0.4734778210776298 1103 20-21 0.27849414170047737 1103 22-23 0.12509924657177862 1103 24-25 0.4878130057009997 1103 26-27 0.05552156612345982 1103 28-29 0.5043774706431634 1103 30-31 0.35225756228226857 1103 32-33 0.5986736486637767 1103 34-35 0.7435453835055981 1103 36-37 1.2534408906856669 1103 38-39 1.5823492465782678 1103 40-41 1.4586178503700538 1103 42-43 1.5546066401353968 1103 44-45 2.029807980666483 1103 46-47 2.5887480615070544 1103 48-49 2.7075181301926676 1103 50-51 2.4209649369727515 1103 52-53 2.211881658009119 1103 54-55 2.724350102038052 1103 56-57 2.977018127989716 1103 58-59 3.0622371595436704 1103 60-61 2.9017437323653716 1103 62-63 3.0891385046494513 1103 64-65 3.3459591899832226 1103 66-67 3.4627273748138343 1103 68-69 3.3502877243845823 1103 70-71 3.3709026649866125 1103 72-73 3.4148323792093045 1103 74-75 3.4461073748057878 1103 76-77 3.6989613745766476 1103 78-79 3.4640861892131625 1103 80-81 3.4611133659052378 1103 82-83 2.951028812568744 1103 84-85 3.1649413661901207 1103 86-87 2.8227039146869295 1103 88-89 3.4185042400907015 1103 90-91 3.0786163396551807 1103 92-93 3.0579662256338196 1103 94-95 2.836886458074627 1103 96-97 3.6344223643473974 1103 98-99 3.674126427389375 1103 100-101 3.893518839055858 1104 1 0.010416985896831932 1104 2 -0.3832406129184065 1104 3 0.014662229378416214 1104 4 0.0669965166359674 1104 5 0.013546862021819095 1104 6 -0.408508662155846 1104 7 -0.14587054363985175 1104 8 -0.19592291942037576 1104 9 -0.21993852435678463 1104 10-11 -0.2010941419413399 1104 12-13 0.2790996581796108 1104 14-15 -0.12481855819775944 1104 16-17 0.14001307601116508 1104 18-19 -0.8234670373426383 1104 20-21 -0.5985550386273886 1104 22-23 -0.4197032869602637 1104 24-25 -0.5727309585314941 1104 26-27 0.09501485971511414 1104 28-29 -0.35488109865639217 1104 30-31 -0.3584354332467896 1104 32-33 -0.1516989295776554 1104 34-35 -0.9629561068073613 1104 36-37 -0.4337796756332608 1104 38-39 -0.5374346580416969 1104 40-41 -0.009712999108337783 1104 42-43 0.10257608871960144 1104 44-45 0.8166000820077599 1104 46-47 0.40814075897352353 1104 48-49 0.16700024643707678 1104 50-51 0.4166802871963 1104 52-53 0.3674330738064384 1104 54-55 0.47453639115875745 1104 56-57 0.8527632844725801 1104 58-59 1.247408545534725 1104 60-61 1.0174292763296044 1104 62-63 1.2152562393737476 1104 64-65 1.494897341995145 1104 66-67 1.1601938427721095 1104 68-69 0.553901733326466 1104 70-71 0.5859734548524855 1104 72-73 0.6359575654984262 1104 74-75 0.039810802525614974 1104 76-77 -0.5576518892087492 1104 78-79 -0.7594607556452573 1104 80-81 -0.23216159683693505 1104 82-83 -0.4633713364625507 1104 84-85 -0.8864744311273185 1104 86-87 0.11443267772716581 1104 88-89 -0.3675427047677182 1104 90-91 0.5607325840665105 1104 92-93 0.05321585305557974 1104 94-95 -0.4184227818657611 1104 96-97 -0.31239209168837334 1104 98-99 0.0815407343938439 1104 100-101 -0.15379859760584225 1105 1 0.3093981771194052 1105 2 0.5497531174891179 1105 3 0.06756191589879279 1105 4 0.6046140714949004 1105 5 0.42969106264877865 1105 6 0.37205560116704106 1105 7 0.5794429359839768 1105 8 0.44866955706865497 1105 9 0.8168953314425877 1105 10-11 0.8085061715382906 1105 12-13 0.5861137647626933 1105 14-15 0.7098209402348488 1105 16-17 1.0639942672337313 1105 18-19 1.04173672441285 1105 20-21 1.0135139268898499 1105 22-23 0.8673264936040042 1105 24-25 0.2834602640390429 1105 26-27 0.5354537311884684 1105 28-29 0.8819919420959579 1105 30-31 0.979730710953838 1105 32-33 0.9799625124286138 1105 34-35 -0.5348604955221035 1105 36-37 -0.6920085157586442 1105 38-39 -0.4367293288880987 1105 40-41 -0.1405907420550463 1105 42-43 0.27832060282619153 1105 44-45 0.312877511474845 1105 46-47 0.6642144266851204 1105 48-49 0.7804406853085482 1105 50-51 0.4954420426821855 1105 52-53 0.6617120706716477 1105 54-55 0.11129187705218158 1105 56-57 0.46444040046004176 1105 58-59 0.2858097994532187 1105 60-61 0.735689464417387 1105 62-63 0.15216846507907533 1105 64-65 0.8179772792992175 1105 66-67 1.4485950966906067 1105 68-69 1.027647814831166 1105 70-71 1.5089750222506062 1105 72-73 1.956098631329148 1105 74-75 1.65540641381088 1105 76-77 1.6521835339887403 1105 78-79 1.883956172254429 1105 80-81 1.5348838577085182 1105 82-83 1.776636500528042 1105 84-85 1.7052183588413357 1105 86-87 1.8080063454387663 1105 88-89 1.2263052576147295 1105 90-91 1.6046197314019288 1105 92-93 2.018537169670001 1105 94-95 0.7831838012063379 1105 96-97 0.4080232688131957 1105 98-99 -0.11007368566885134 1105 100-101 0.07582522684556992 1106 1 0.15183112731097737 1106 2 0.6963048416270468 1106 3 0.7318339465501325 1106 4 -0.1362863116468631 1106 5 0.10066807414303014 1106 6 0.21017820653102604 1106 7 0.4185233957540859 1106 8 0.16897607047861385 1106 9 -0.21615064556890218 1106 10-11 0.14423414088695097 1106 12-13 0.13137238545234453 1106 14-15 0.4698784114992094 1106 16-17 0.2814272174253034 1106 18-19 -0.21424986562546877 1106 20-21 -0.09855503862738857 1106 22-23 0.03926135950438692 1106 24-25 -0.3738673221678539 1106 26-27 -0.014833625133377382 1106 28-29 -0.06005786633316035 1106 30-31 0.040554465743106505 1106 32-33 0.28264450476577707 1106 34-35 0.528836822485566 1106 36-37 0.3168516374980541 1106 38-39 0.5553683722613343 1106 40-41 0.6960950816997453 1106 42-43 0.930858917002432 1106 44-45 1.209908162815843 1106 46-47 1.199176112508873 1106 48-49 1.6865709535077826 1106 50-51 1.6130186710346806 1106 52-53 1.9873825687559403 1106 54-55 1.7434757850981555 1106 56-57 1.7271319713412652 1106 58-59 1.50372167684786 1106 60-61 0.9246262460265804 1106 62-63 1.655281491898993 1106 64-65 0.4286094632072661 1106 66-67 0.2012291963074624 1106 68-69 0.4213259757507082 1106 70-71 0.2917815356605651 1106 72-73 0.08229594933681383 1106 74-75 0.955214842929653 1106 76-77 1.0616662926094307 1106 78-79 0.8604887393042375 1106 80-81 0.3334949688196289 1106 82-83 -0.9968309324221494 1106 84-85 -0.933191602844488 1106 86-87 -0.5875875242930348 1106 88-89 -0.6920376542626663 1106 90-91 -0.9032825674486453 1106 92-93 -0.4158498035100777 1106 94-95 -0.2706955091384877 1106 96-97 -0.7107506775469581 1106 98-99 -0.1646713868182772 1106 100-101 -0.3532935471007903 1107 1 0.17552393776849584 1107 2 0.29924601809763374 1107 3 0.38297773739980556 1107 4 0.47073983214399107 1107 5 -0.2008025140922598 1107 6 -0.4254427085016559 1107 7 -0.72853542777532 1107 8 -0.06958602102465505 1107 9 0.2617905309016848 1107 10-11 0.02740407552747115 1107 12-13 0.10318611094253782 1107 14-15 0.46742743110705476 1107 16-17 0.7830612043534089 1107 18-19 -0.022664898305201575 1107 20-21 0.05340574568634082 1107 22-23 0.2606665882625592 1107 24-25 0.1739267954792041 1107 26-27 -0.20846107611376397 1107 28-29 -0.3157768205815259 1107 30-31 0.02993355071043169 1107 32-33 0.011402674700420334 1107 34-35 -0.2415880141157416 1107 36-37 0.2821294152758327 1107 38-39 -0.4997786865622018 1107 40-41 -0.2998199509799875 1107 42-43 0.01868571438804878 1107 44-45 -0.5016931443736965 1107 46-47 -0.09575852801400231 1107 48-49 -0.06506303342031572 1107 50-51 -0.3718669498803564 1107 52-53 -0.8917023985643269 1107 54-55 -1.6094653913724315 1107 56-57 -2.122949728005139 1107 58-59 -2.441539761060646 1107 60-61 -1.9908149304440101 1107 62-63 -2.0657152401271475 1107 64-65 -1.6228611250280274 1107 66-67 -1.4454211304899225 1107 68-69 -0.6620073575826275 1107 70-71 -0.9700648695681906 1107 72-73 -1.0006288872644937 1107 74-75 -1.2081838498808004 1107 76-77 -0.705490570135666 1107 78-79 -0.7857530907611228 1107 80-81 -0.4111945736640301 1107 82-83 0.008888021826216175 1107 84-85 0.1558606847372097 1107 86-87 -0.1333391582799628 1107 88-89 0.10493946992033898 1107 90-91 -0.3603904105858966 1107 92-93 0.08986915073828783 1107 94-95 0.09082409870464758 1107 96-97 0.05558919173408938 1107 98-99 -0.6058478574065127 1107 100-101 -0.22420857978053021 1108 1 0.8029749181606505 1108 2 0.612971508293711 1108 3 0.4221934236743152 1108 4 0.529563361555752 1108 5 0.5639033682606822 1108 6 0.6725965071846218 1108 7 0.8400920232050666 1108 8 0.812766920151816 1108 9 0.9676728838428588 1108 10-11 0.8313256441549228 1108 12-13 0.5247547383935185 1108 14-15 1.1046823330678421 1108 16-17 1.0379631651377181 1108 18-19 0.840080199734011 1108 20-21 0.8181116280392828 1108 22-23 0.9273332549292164 1108 24-25 1.2719660111654747 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0.8132728032536711 2314 90-91 0.5807860600023353 2314 92-93 0.312908366424562 2314 94-95 0.9682750790968093 2314 96-97 1.4673538976164373 2314 98-99 1.5461129269072167 2314 100-101 0.8468698515920181 2315 1 0.1723866828665308 2315 2 0.03963817496038047 2315 3 0.04572283543902245 2315 4 0.4409359105753623 2315 5 0.08233474080969927 2315 6 0.3024004287532449 2315 7 0.2251900624207579 2315 8 0.3311982927008401 2315 9 0.5621826877644267 2315 10-11 0.8270119186647236 2315 12-13 0.5522057187856788 2315 14-15 0.8282117448325437 2315 16-17 1.1269827729808597 2315 18-19 0.6671390232634238 2315 20-21 0.4581116280392834 2315 22-23 0.7920391372821669 2315 24-25 0.745299344498811 2315 26-27 0.9601663748666311 2315 28-29 0.35049768922239366 2315 30-31 0.9644433546319959 2315 32-33 1.1670889492102248 2315 34-35 1.0960590447077863 2315 36-37 1.1087960819425007 2315 38-39 1.1778683722613295 2315 40-41 1.776650637255301 2315 42-43 1.2128033614468734 2315 44-45 2.0371303850380684 2315 46-47 2.5097316680644255 2315 48-49 3.231015397952234 2315 50-51 2.2410742265902286 2315 52-53 2.4651603465337146 2315 54-55 2.8034757850981578 2315 56-57 3.1868541935634838 2315 58-59 2.7498327879589652 2315 60-61 1.4221262460265756 2315 62-63 1.373892603010109 2315 64-65 2.1202761298739325 2315 66-67 2.025951418529683 2315 68-69 1.597992642417374 2315 70-71 1.7981704245494576 2315 72-73 2.010351504892366 2315 74-75 1.7529926207074311 2315 76-77 1.593332959276097 2315 78-79 1.9432665170820158 2315 80-81 1.5448838577085198 2315 82-83 2.3026135120222975 2315 84-85 2.5229195082666216 2315 86-87 1.6968569201514079 2315 88-89 2.361017901292886 2315 90-91 2.6858840992180255 2315 92-93 2.4035946409343687 2315 94-95 3.101804490861511 2315 96-97 2.981471544675262 2315 98-99 2.4686619465150557 2315 100-101 2.472262008454763 2316 1 0.05059974128577949 2316 2 -0.7237982511564596 2316 3 -0.13915688964688755 2316 4 -0.13872044681295392 2316 5 0.144052953868119 2316 6 0.0793076452480932 2316 7 0.3137123991905142 2316 8 -0.6517570337596439 2316 9 -0.21829841189192933 2316 10-11 0.007493018321085287 2316 12-13 0.21062496277193787 2316 14-15 -0.40855801461762553 2316 16-17 -0.38552581808443875 2316 18-19 -0.516297402853418 2316 20-21 -0.2918540076995484 2316 22-23 -0.03304677337076356 2316 24-25 0.2685295850486398 2316 26-27 0.22786396936834308 2316 28-29 -0.2514954379253709 2316 30-31 -0.30724049416525645 2316 32-33 0.03808551278410732 2316 34-35 -0.6686144948111163 2316 36-37 -1.0116850177138588 2316 38-39 -1.646736438735239 2316 40-41 -1.5099473008890314 2316 42-43 -1.5428667416459092 2316 44-45 -2.310567209463649 2316 46-47 -2.2310930742036064 2316 48-49 -2.599225151875949 2316 50-51 -2.71652027512798 2316 52-53 -2.827623158620927 2316 54-55 -3.3252527372386105 2316 56-57 -3.015207662106615 2316 58-59 -3.13731497836406 2316 60-61 -2.801103994523249 2316 62-63 -2.9739424485362846 2316 64-65 -3.195531430263525 2316 66-67 -2.938447206899866 2316 68-69 -2.677471275108399 2316 70-71 -2.7203175479591373 2316 72-73 -2.7627069143516216 2316 74-75 -2.468038307127621 2316 76-77 -2.210240923885415 2316 78-79 -2.6748778128148913 2316 80-81 -2.3646006783739573 2316 82-83 -2.039929443304164 2316 84-85 -1.884365715101076 2316 86-87 -1.9507032173056338 2316 88-89 -2.3725216176074575 2316 90-91 -2.1517791310225256 2316 92-93 -2.26479023879072 2316 94-95 -1.849948086458074 2316 96-97 -0.9601779398608201 2316 98-99 -1.1371799778835694 2316 100-101 -0.44292699498166144 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 521.0 3 81.0 4 84.0 5 89.0 6 97.0 7 99.0 8 116.0 9 162.0 10 181.0 11 275.0 12 444.0 13 777.0 14 838.0 15 1217.0 16 1837.0 17 2709.0 18 2165.0 19 1194.0 20 1061.0 21 1073.0 22 1076.0 23 1125.0 24 1201.0 25 1279.0 26 1380.0 27 1466.0 28 1619.0 29 1867.0 30 2324.0 31 2830.0 32 3540.0 33 4758.0 34 6684.0 35 10419.0 36 14797.0 37 22038.0 38 19965.0 39 3011.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78515258266509 20.864592036244304 16.01794988845919 19.332305492631416 2 14.502475780409041 21.80925726587729 42.212271259418735 21.47599569429494 3 17.362402700282427 29.88306812702349 31.504443066749328 21.250086105944753 4 10.684058719703819 18.709371905807394 39.83382840414998 30.772740970338802 5 10.580101935395 39.6833459604656 34.79664577450238 14.939906329637026 6 25.892680407934936 40.27023538017987 18.525754120228928 15.311330091656266 7 24.465997693591973 36.75278404103341 22.185407666224894 16.595810599149726 8 25.177298860467516 34.234172203669914 20.745688022859504 19.842840913003066 9 27.14104980506571 16.723038393018513 21.966039262261926 34.16987253965385 10-11 22.970036276631912 29.02517847844703 27.044379704020553 20.960405540900503 12-13 25.232374001652435 27.703714541448637 28.45204489121454 18.611866565684384 14-15 22.444790608809406 27.402877945504933 29.310463535122295 20.84186791056337 16-17 17.871142914925752 31.46254039423917 29.69401498298171 20.972301707853372 18-19 17.721856698409148 27.862955105834565 36.108971517829865 18.306216677926425 20-21 19.180162814312514 26.600175550315818 36.13152505034164 18.088136585030032 22-23 18.843173614308025 26.877455050789706 36.14178953572919 18.13758179917308 24-25 18.090841425479827 27.350953202510897 36.493178503358045 18.06502686865123 26-27 17.890551716422408 27.303981893443257 36.52507293396787 18.28039345616647 28-29 17.353885345933058 27.07004724897369 37.45406349780107 18.12200390729218 30-31 17.415362902531797 27.43627476291286 36.97397635152579 18.174385983029552 32-33 17.242136064374545 27.965058737467192 36.46714574637463 18.32565945178364 34-35 17.66459980120225 27.720237352461517 36.35416980425737 18.26099304207886 36-37 17.727542617181115 27.64114655491399 35.90771799086129 18.7235928370436 38-39 17.339861632189447 28.077995387739644 36.26303651946443 18.319106460606477 40-41 17.255369312315 28.016796310318714 36.383544434501275 18.34428994286501 42-43 17.509950302274046 28.11901637228115 35.46997697984123 18.901056345603582 44-45 17.356676935786446 28.572965251363957 34.9775398860644 19.09281792678519 46-47 17.99272821152729 28.67580301628622 34.261311073341794 19.07015769884469 48-49 17.876397870976348 29.028385547767492 33.48995512183368 19.60526145942248 50-51 18.20575933352841 29.120264385424377 33.07055441761192 19.603421863435287 52-53 18.256270334475218 29.516620474772342 32.75033998381849 19.476769206933948 54-55 18.111682902481842 29.24038759423084 32.77339850607552 19.8745309972118 56-57 18.193980796365764 29.50450838008053 32.35838180128712 19.94312902226658 58-59 18.096409797561794 28.624400987671315 33.02246350003872 20.256725714728173 60-61 18.326249311674008 28.999604212555063 33.05685572687225 19.61729074889868 62-63 18.483526506449145 28.611562852250533 33.42884431709647 19.47606632420386 64-65 18.446263642190665 28.149910306763775 33.618401524569926 19.785424526475637 66-67 18.383510725256194 28.5180819301159 32.95402724120426 20.14438010342365 68-69 18.36344863190501 28.23309241094476 33.32515918086388 20.078299776286354 70-71 18.285888826270075 28.04682357698744 33.42138207849531 20.24590551824717 72-73 18.24795223017621 27.99292745044053 33.210008259911895 20.549112059471366 74-75 18.00401829312889 28.178388122372944 33.13887203844483 20.67872154605334 76-77 17.967297762478484 27.845524956970742 33.526678141135974 20.6604991394148 78-79 17.777949902964426 27.388990012436043 33.424129369290284 21.408930715309243 80-81 17.816435680201714 27.940398177351135 33.10198830510006 21.14117783734709 82-83 18.121582142595916 27.745149923199047 32.98611559295927 21.147152341245768 84-85 17.62362436434662 27.551433070324126 32.55965031535291 22.265292249976337 86-87 17.625673326793674 27.693307174694958 32.78821828695338 21.892801211557988 88-89 18.02938708775251 26.995245573650585 32.72077963986834 22.254587698728567 90-91 17.669559530314828 27.20568303565666 33.18689734995331 21.937860084075194 92-93 17.993133180163667 27.13985767268159 32.966758740566725 21.900250406588018 94-95 17.33004048652672 27.07262190058643 32.9223011491978 22.67503646368905 96-97 17.70719052105786 27.35908721297934 32.4677000916401 22.466022174322703 98-99 17.929699264294626 27.86645441638343 31.646517230994277 22.55732908832767 100-101 18.217797929913015 27.32626670222926 31.773168024641436 22.68276734321629 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 253.0 1 170.0 2 116.0 3 145.0 4 112.0 5 107.5 6 213.0 7 520.0 8 920.0 9 1024.0 10 766.5 11 541.0 12 515.5 13 509.5 14 506.5 15 518.5 16 522.5 17 521.5 18 545.5 19 555.0 20 562.5 21 616.0 22 697.0 23 782.5 24 844.5 25 983.0 26 1160.0 27 1336.5 28 1476.0 29 1676.0 30 1956.5 31 2198.5 32 2615.5 33 3155.5 34 3674.5 35 4019.5 36 4629.0 37 5226.5 38 5065.5 39 4786.5 40 4513.5 41 4241.0 42 4006.5 43 3917.5 44 3944.5 45 3928.5 46 3641.0 47 3337.5 48 3655.0 49 3503.0 50 2869.0 51 2442.0 52 2111.5 53 1980.5 54 1900.5 55 1707.5 56 1455.0 57 1224.5 58 1078.5 59 957.0 60 813.5 61 690.5 62 559.0 63 412.5 64 277.0 65 210.0 66 148.5 67 110.0 68 90.0 69 58.0 70 39.0 71 28.0 72 27.5 73 27.0 74 18.0 75 9.5 76 11.5 77 9.5 78 4.5 79 4.5 80 4.0 81 1.5 82 2.0 83 2.5 84 1.0 85 2.0 86 2.0 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.256015945154168 2 0.23539721131624844 3 0.22594695830720196 4 0.2182149331179821 5 0.2122011357485889 6 0.17525923762231635 7 0.1726818958925764 8 0.18127303499170955 9 0.176977465442143 10-11 0.17912525021692627 12-13 0.1778365793520563 14-15 0.17611835153222966 16-17 0.17225233893761974 18-19 0.17482968066735968 20-21 0.16580898461326987 22-23 0.1585065163790067 24-25 0.15936563028892 26-27 0.17010455416283646 28-29 0.176977465442143 30-31 0.16924544025292315 32-33 0.17525923762231635 34-35 0.17225233893761974 36-37 0.16323164288352993 38-39 0.16065430115378998 40-41 0.1572178455141367 42-43 0.16881588329796648 44-45 0.16580898461326987 46-47 0.16881588329796648 48-49 0.16795676938805318 50-51 0.17611835153222966 52-53 0.18642771845118944 54-55 0.1675272124330965 56-57 0.14690847859517694 58-59 0.14261290904561036 60-61 0.1503449342348302 62-63 0.1563587316042234 64-65 0.14561980773030697 66-67 0.15292227596457014 68-69 0.15378138987448345 70-71 0.15077449118978686 72-73 0.1503449342348302 74-75 0.15507006073935342 76-77 0.17096366807274976 78-79 0.17568879457727302 80-81 0.16709765547813984 82-83 0.16280208592857326 84-85 0.15464050378439678 86-87 0.15893607333396337 88-89 0.1645203137483999 90-91 0.16623854156822654 92-93 0.16151341506370331 94-95 0.16108385810874665 96-97 0.15764740246909337 98-99 0.15807695942405003 100-101 0.14776759250509025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 116399.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.444660177492935 #Duplication Level Percentage of deduplicated Percentage of total 1 84.23466462832027 49.23066349367263 2 7.8437137103294186 9.168463646594901 3 2.5327433888488735 4.440759800341927 4 1.3082655926149143 3.0584455192914026 5 0.8378779638095518 2.448474643252949 6 0.6232636081671052 2.1855857868194746 7 0.4498081700451278 1.8402219950343215 8 0.3160416881036029 1.4776759250509026 9 0.25871319584294933 1.3608364333026917 >10 1.4934807214570256 15.281918229538055 >50 0.08231783504093843 3.2268318456344125 >100 0.014699613400167576 1.9209787025661733 >500 0.0 0.0 >1k 0.004409884020050273 4.359143978900162 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2256 1.9381609807644398 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1596 1.3711458002216514 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1222 1.0498371979140715 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 477 0.40979733502865145 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 378 0.32474505794723324 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 330 0.28350759027139405 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 196 0.16838632634300982 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 173 0.14862670641500356 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 169 0.1451902507753503 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 164 0.14089468122578372 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 128 0.10996658046890438 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 120 0.10309366918959785 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 8.591139099133154E-4 0.0 4 0.0 0.0 0.0 8.591139099133154E-4 0.0 5 0.0 0.0 0.0 8.591139099133154E-4 0.0 6 0.0 0.0 0.0 8.591139099133154E-4 0.0 7 0.0 0.0 0.0 8.591139099133154E-4 0.0 8 0.0 0.0 0.0 8.591139099133154E-4 0.0 9 0.0 0.0 0.0 8.591139099133154E-4 0.0 10-11 0.0 0.0 0.0 8.591139099133154E-4 0.0 12-13 0.0 0.0 0.0 8.591139099133154E-4 0.0 14-15 0.0 0.0 0.0 8.591139099133154E-4 0.0 16-17 0.0 0.0 0.0 0.001288670864869973 0.0 18-19 0.0 0.0 0.0 0.0017182278198266307 0.0 20-21 0.0 0.0 0.0 0.0017182278198266307 0.0 22-23 0.0 0.0 0.0 0.0025773417297399463 0.0 24-25 0.0 0.0 0.0 0.003006898684696604 0.0 26-27 0.0 0.0 0.0 0.006013797369393208 0.0 28-29 0.0 0.0 0.0 0.014604936468526362 0.0 30-31 0.0 0.0 0.0 0.02835075902713941 0.0 32-33 0.0 0.0 0.0 0.0416670246307958 0.0 34-35 0.0 0.0 0.0 0.05884930282906211 0.0 36-37 0.0 0.0 0.0 0.07216556843271849 0.0 38-39 0.0 0.0 0.0 0.08762961881115816 0.0 40-41 0.0 0.0 0.0 0.1026641122346412 0.0 42-43 0.0 0.0 0.0 0.13359221299152055 0.0 44-45 0.0 0.0 0.0 0.18986417409084272 0.0 46-47 0.0 0.0 0.0 0.2517203756046014 0.0 48-49 0.0 0.0 0.0 0.39304461378534183 0.0 50-51 0.0 0.0 0.0 0.5081658777137261 0.0 52-53 0.0 0.0 0.0 0.63488517942594 0.0 54-55 0.0 0.0 0.0 0.8488045429943556 0.0 56-57 0.0 0.0 0.0 1.0738923873916444 0.0 58-59 0.0 0.0 0.0 1.3380699146899886 0.0 60-61 0.0 0.0 0.0 1.6464918083488689 0.0 62-63 0.0 0.0 0.0 2.122440914440846 0.0 64-65 0.0 0.0 0.0 2.4252785676852895 0.0 66-67 0.0 0.0 0.0 2.8466739404977703 0.0 68-69 0.0 0.0 0.0 3.3419531095627972 0.0 70-71 0.0 0.0 0.0 3.777953418843804 0.0 72-73 0.0 0.0 0.0 4.408972585675135 0.0 74-75 0.0 0.0 0.0 5.1387898521464965 0.0 76-77 0.0 0.0 0.0 5.75649275337417 0.0 78-79 0.0 0.0 0.0 6.3106212252682585 0.0 80-81 0.0 0.0 0.0 7.017671973126917 0.0 82-83 0.0 0.0 0.0 7.686062595039476 0.0 84-85 0.0 0.0 0.0 8.361326128231344 0.0 86-87 0.0 0.0 0.0 9.438225414307684 0.0 88-89 0.0 0.0 0.0 10.741071658691226 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 860 0.0 38.15892 1 GAGTACC 50 0.0015998087 38.048317 1 CCTGACC 65 1.3143255E-4 36.569145 3 ACATAAG 55 0.0025595531 34.574463 3 GTATCAA 1310 0.0 32.675083 1 TACTCCC 60 0.0039285915 31.679598 5 GACAGAT 80 4.4696554E-4 29.686827 7 AGGCCTG 65 0.0058111153 29.242704 5 ATTATAC 85 6.341028E-4 27.96464 3 CAGTGCA 90 8.900144E-4 26.38829 9 CATGGGG 165 8.241841E-8 25.919672 4 GAGTACT 1050 0.0 25.785131 12-13 ATCAACG 1670 0.0 25.335629 3 TATCAAC 1685 0.0 25.11009 2 CATGGGC 95 0.001220081 24.999434 6 TCAACGC 1675 0.0 24.965414 4 CAACGCA 1710 0.0 24.454426 5 AACGCAG 1725 0.0 24.231335 6 GTACATG 945 0.0 24.157661 1 ATGGGAC 100 0.0016449512 23.74946 7 >>END_MODULE