FastQCFastQC Report
Thu 26 May 2016
SRR937705_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937705_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116399
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19211.650357820943479No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15481.3299083325458123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11641.000008591139099No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9600.8247493535167828No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4570.39261505683038517No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3100.26632531207312776No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.16151341506370331No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA1670.1434720229555237No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA1660.14261290904561036No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1660.14261290904561036No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG1510.12972620039691063No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG1480.12714885866717068No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1380.11855771956803753No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.11683949174821089No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT1350.11598037783829758No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA1350.11598037783829758No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.11512126392838426No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG1310.11254392219864431No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.10824835264907774No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC1170.1005163274598579No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTCG656.6029315E-1058.4763539
GGGTATG250.005004942457.0144467
GGGCGTA250.005004942457.0144469
AGAGGGC250.005004942457.0144468
GTACCAC250.005013469656.9899446
CGTATAG250.005013469656.9899441
CCCTGGT701.2751116E-954.299477
TAGATAT608.2325394E-539.593377
GGTATCA11300.039.5063061
CCTGGTC1054.586036E-836.1996468
ATAACCC1054.6034984E-836.184093
AGATATA550.002566950634.554218
TGTAGTA550.002566950634.554217
AGTGTGG550.002566950634.554218
TGTAGAT550.002572371634.539365
AATAACC1106.931441E-834.539362
CGTAGCT753.0570404E-431.6746948
TAGTAGC600.003931612731.6746949
GTTCTGA753.0647655E-431.6610811
GTCTAAG300.003947943431.6610872-73
Produced by FastQC (version 0.11.5)