##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937705_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 116399 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41852593235337 33.0 31.0 34.0 31.0 34.0 2 32.596783477521285 34.0 31.0 34.0 31.0 34.0 3 32.62905179597763 34.0 31.0 34.0 31.0 34.0 4 36.20897086744731 37.0 35.0 37.0 35.0 37.0 5 36.166350226376515 37.0 35.0 37.0 35.0 37.0 6 36.243155009922766 37.0 37.0 37.0 35.0 37.0 7 36.206969132037216 37.0 36.0 37.0 35.0 37.0 8 36.192501653794274 37.0 36.0 37.0 35.0 37.0 9 37.88231857662007 39.0 38.0 39.0 35.0 39.0 10-11 37.8300371996323 39.0 38.0 39.0 35.0 39.0 12-13 37.72174159571817 39.0 37.0 39.0 35.0 39.0 14-15 38.85201762901743 40.0 38.0 41.0 35.5 41.0 16-17 38.85987422572359 40.0 38.0 41.0 35.5 41.0 18-19 38.93937662694697 40.0 38.0 41.0 35.5 41.0 20-21 38.924269108841145 40.0 38.0 41.0 35.0 41.0 22-23 38.85072466258301 40.0 38.0 41.0 35.0 41.0 24-25 38.830273455957524 40.0 38.0 41.0 35.0 41.0 26-27 38.594970747171374 40.0 38.0 41.0 35.0 41.0 28-29 38.515902198472496 40.0 38.0 41.0 34.5 41.0 30-31 38.376377803933025 40.0 37.5 41.0 35.0 41.0 32-33 38.10686088368457 40.0 36.5 41.0 34.0 41.0 34-35 37.87157106160706 40.0 36.0 41.0 34.0 41.0 36-37 37.63538346549369 40.0 35.5 41.0 33.0 41.0 38-39 37.4048617256162 40.0 35.0 41.0 33.0 41.0 40-41 37.11566250569163 40.0 35.0 41.0 33.0 41.0 42-43 36.74867481679396 40.0 35.0 41.0 32.5 41.0 44-45 36.19807730306961 39.5 35.0 41.0 30.5 41.0 46-47 35.486640778700846 39.0 35.0 41.0 27.5 41.0 48-49 34.82691002500022 39.0 35.0 41.0 22.5 41.0 50-51 34.44609919329204 39.0 35.0 41.0 19.0 41.0 52-53 34.15305543862061 39.0 35.0 40.5 13.5 41.0 54-55 33.85893349599223 38.5 34.0 40.0 8.0 41.0 56-57 33.56264229074133 38.0 34.0 40.0 3.5 41.0 58-59 33.26849027912611 38.0 34.0 40.0 2.0 41.0 60-61 32.8993676921623 38.0 33.0 40.0 2.0 41.0 62-63 32.46210878100327 37.0 33.0 40.0 2.0 41.0 64-65 32.048380999836766 36.5 32.5 40.0 2.0 41.0 66-67 31.57691646835454 36.0 32.0 39.0 2.0 41.0 68-69 31.07740616328319 35.5 31.0 39.0 2.0 40.0 70-71 30.621233859397417 35.0 31.0 38.5 2.0 40.0 72-73 30.11685667402641 35.0 30.0 38.0 2.0 40.0 74-75 29.596684679421642 35.0 29.5 37.0 2.0 39.0 76-77 28.36612427941821 33.5 28.0 35.5 2.0 38.5 78-79 28.85172982585761 34.0 29.0 36.0 2.0 39.0 80-81 28.62677514411636 34.0 29.0 36.0 2.0 37.5 82-83 28.21584807429617 34.0 29.0 35.0 2.0 37.0 84-85 27.782605520665985 34.0 27.5 35.0 2.0 37.0 86-87 27.435764052955783 34.0 27.0 35.0 2.0 36.0 88-89 27.034231393740498 34.0 26.0 35.0 2.0 36.0 90-91 26.559317519909964 33.5 25.0 35.0 2.0 35.5 92-93 26.14021598123695 33.0 24.0 35.0 2.0 35.0 94-95 25.66283215491542 33.0 21.5 35.0 2.0 35.0 96-97 25.183927696973342 33.0 19.0 35.0 2.0 35.0 98-99 24.46215173669877 32.0 2.0 34.5 2.0 35.0 100-101 22.268644919629892 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3648951886117402 1101 2 0.5049296621913726 1101 3 0.4533483691482232 1101 4 0.09502009460894811 1101 5 -0.04188140885430869 1101 6 0.09380148431294799 1101 7 0.2932983167858936 1101 8 0.32614386102245163 1101 9 0.28711313775870195 1101 10-11 0.34598215582590086 1101 12-13 0.2722341890155917 1101 14-15 0.3900230713206341 1101 16-17 0.12528871757589144 1101 18-19 0.21780882885969532 1101 20-21 0.4163000275866864 1101 22-23 0.3537641798145046 1101 24-25 0.257356284631463 1101 26-27 0.3068895275675274 1101 28-29 0.5664089186338117 1101 30-31 0.47567834764068095 1101 32-33 0.728765033066658 1101 34-35 0.7084693021784645 1101 36-37 0.7744263607899384 1101 38-39 0.6577104814810895 1101 40-41 0.7291390129034312 1101 42-43 1.0034942839874361 1101 44-45 0.7877353531989897 1101 46-47 1.0411878434997632 1101 48-49 1.2474909284281566 1101 50-51 0.27635466024680966 1101 52-53 0.5882145393295133 1101 54-55 0.9200522447258024 1101 56-57 1.0106212990153551 1101 58-59 1.1294024738857473 1101 60-61 0.7635773363136593 1101 62-63 0.7948447654132913 1101 64-65 0.9054734801246731 1101 66-67 0.8668460135158078 1101 68-69 0.8532754963938878 1101 70-71 0.5296952753256221 1101 72-73 0.9711513949279862 1101 74-75 0.8784967700678799 1101 76-77 0.7908034597709594 1101 78-79 1.3374506106318265 1101 80-81 1.1283148885839367 1101 82-83 0.8885223004348113 1101 84-85 1.491160962861649 1101 86-87 1.2774348306145598 1101 88-89 1.57964704612494 1101 90-91 1.907276904146535 1101 92-93 1.4027369170153783 1101 94-95 1.1992516283317975 1101 96-97 1.446464545430544 1101 98-99 2.089988171169864 1101 100-101 2.3511950947838685 1102 1 0.27830810996005084 1102 2 0.25563190938238733 1102 3 0.2502584815077782 1102 4 0.3072391957325422 1102 5 0.3057309506962511 1102 6 0.22111890004328671 1102 7 0.30516629431398457 1102 8 0.2080258834943649 1102 9 0.22601763214072434 1102 10-11 0.23239367267983368 1102 12-13 0.16219205418413196 1102 14-15 0.17678571177006575 1102 16-17 -0.038826450963433956 1102 18-19 0.14424843560126988 1102 20-21 0.2522901961260118 1102 22-23 0.4561167079043926 1102 24-25 0.4627635880022538 1102 26-27 -0.14044193310662934 1102 28-29 0.6499061096450518 1102 30-31 0.4672864937081016 1102 32-33 0.6399307634037328 1102 34-35 0.6708990774593602 1102 36-37 0.5081342259584787 1102 38-39 0.281902897211431 1102 40-41 0.23904069829669083 1102 42-43 0.7466473738750778 1102 44-45 0.645319622861912 1102 46-47 0.1315670569829095 1102 48-49 0.5218589059562433 1102 50-51 0.6530049411456886 1102 52-53 0.6726246516890626 1102 54-55 1.0319904469729977 1102 56-57 0.8129036023861431 1102 58-59 1.1351960132115906 1102 60-61 1.5836967183361423 1102 62-63 1.4359964508065524 1102 64-65 1.430894828439282 1102 66-67 1.9947252269989555 1102 68-69 1.9202698784163523 1102 70-71 2.1600674663368515 1102 72-73 2.287970215152704 1102 74-75 2.1165234554611345 1102 76-77 1.5909439092091624 1102 78-79 2.197633194901492 1102 80-81 2.6416224728536015 1102 82-83 2.2886276375134607 1102 84-85 2.1692157381425474 1102 86-87 2.070447471064 1102 88-89 1.8707285067990966 1102 90-91 2.097726342348782 1102 92-93 2.1274209057794238 1102 94-95 2.6086968530509047 1102 96-97 2.561211736441784 1102 98-99 2.452417946450762 1102 100-101 2.1225785217501603 1103 1 0.2411154870092318 1103 2 0.19723027003812632 1103 3 0.3760781536389217 1103 4 0.21318181868336694 1103 5 0.13872275397493894 1103 6 0.2316721787318059 1103 7 0.41367039267463923 1103 8 0.29593571955993525 1103 9 0.5159766485341635 1103 10-11 0.27824408251589716 1103 12-13 0.04774533287265115 1103 14-15 0.22581235111433529 1103 16-17 0.26988256543000233 1103 18-19 5.496651094603067E-4 1103 20-21 0.324984868257161 1103 22-23 0.19305318331422683 1103 24-25 0.47659555521536845 1103 26-27 0.4630416734507463 1103 28-29 0.321934798169643 1103 30-31 0.04981723141301586 1103 32-33 0.03388568143652293 1103 34-35 -0.0017443651635886681 1103 36-37 0.11960963579453932 1103 38-39 0.07836806114585215 1103 40-41 -0.04466831809674687 1103 42-43 -0.09684647858394158 1103 44-45 -0.427221360744646 1103 46-47 -0.6720190085908584 1103 48-49 -0.6526287989617856 1103 50-51 0.046293875571912224 1103 52-53 0.4617639959513582 1103 54-55 0.11949044697299627 1103 56-57 -0.11850008613843954 1103 58-59 -0.30460931465726304 1103 60-61 0.020530734729575784 1103 62-63 -0.15929043443935242 1103 64-65 0.38806695958682624 1103 66-67 0.5060981778186289 1103 68-69 0.8397370915311129 1103 70-71 0.3139096794516121 1103 72-73 1.3357673463002442 1103 74-75 1.3792796030021215 1103 76-77 1.547296368225556 1103 78-79 1.31843237522936 1103 80-81 1.5806081285913045 1103 82-83 1.2099903424314924 1103 84-85 0.813734180765497 1103 86-87 0.9328962415558024 1103 88-89 1.362019490405654 1103 90-91 1.3673984734963227 1103 92-93 1.6391524631564742 1103 94-95 1.7127439841984433 1103 96-97 0.9683838675893242 1103 98-99 1.1543646677622412 1103 100-101 1.8551092594550767 1104 1 -0.21771461731267294 1104 2 -0.02277718152670616 1104 3 -0.22246879121949803 1104 4 -0.20298807699472832 1104 5 -0.27949632203102226 1104 6 -0.3396765545021694 1104 7 -0.11017461477692336 1104 8 -0.22492866196018468 1104 9 -0.37057327695018216 1104 10-11 -0.05595860004743969 1104 12-13 -0.49519430945223064 1104 14-15 -0.19054383368447958 1104 16-17 -0.4234855418725303 1104 18-19 -0.16683110985327687 1104 20-21 -0.27924389478307887 1104 22-23 -0.2896219284592476 1104 24-25 -0.6309864119977462 1104 26-27 -0.4983964785611761 1104 28-29 -0.11770752671858986 1104 30-31 -0.18015668811008112 1104 32-33 -0.5163192365962672 1104 34-35 -0.03648728617699959 1104 36-37 -0.5839112285869774 1104 38-39 -0.6905402846067616 1104 40-41 -0.4308456653396675 1104 42-43 -0.35988671703401565 1104 44-45 -0.3705894680471786 1104 46-47 -0.34741021574436104 1104 48-49 -0.5196183667710272 1104 50-51 -0.2711427861270437 1104 52-53 -0.3546480755836612 1104 54-55 -0.4941459166633635 1104 56-57 -0.8265850339774943 1104 58-59 -0.7383835322429562 1104 60-61 -0.8603373725729568 1104 62-63 -0.9787762764661743 1104 64-65 -0.5338778988334418 1104 66-67 -0.1297065911828632 1104 68-69 -0.33313921249273193 1104 70-71 0.26603337542776373 1104 72-73 -0.2509502393927505 1104 74-75 -0.23035154453886264 1104 76-77 -0.23064699988174908 1104 78-79 -1.0870258960075994 1104 80-81 -0.2563888907827625 1104 82-83 -0.7298382715774494 1104 84-85 -0.8276592618574519 1104 86-87 -1.29176843802691 1104 88-89 -0.57529422047363 1104 90-91 -0.25341002128758205 1104 92-93 0.0248640875976065 1104 94-95 -0.48760996513091825 1104 96-97 -0.8058337181036705 1104 98-99 -1.2117865990037835 1104 100-101 -0.9657737509771138 1105 1 -0.2792349934882239 1105 2 -0.056868090617612665 1105 3 0.09767227461122019 1105 4 0.23353229918082263 1105 5 0.19581715759280627 1105 6 0.30883441728466465 1105 7 0.3678818115553639 1105 8 0.3722500214253941 1105 9 0.7669659080027955 1105 10-11 0.4322859140591433 1105 12-13 0.4955972265979227 1105 14-15 -0.07181342616096487 1105 16-17 0.24500975593311836 1105 18-19 0.5057786080150635 1105 20-21 0.132785885781189 1105 22-23 0.22174170790439263 1105 24-25 -0.008141584411539782 1105 26-27 0.059989101376132226 1105 28-29 0.21693197171401835 1105 30-31 0.1805408040529244 1105 32-33 0.4082497289209712 1105 34-35 0.33361459470074095 1105 36-37 0.7995135363033015 1105 38-39 1.4244675523838382 1105 40-41 1.4629631120897955 1105 42-43 1.3258499600819746 1105 44-45 1.875492036655018 1105 46-47 2.082644643189809 1105 48-49 2.675522699059691 1105 50-51 1.4475092514905086 1105 52-53 1.3273660309994142 1105 54-55 0.886731826283345 1105 56-57 1.1416751541102883 1105 58-59 2.0118123925219393 1105 60-61 1.9697958562671687 1105 62-63 2.614875761151378 1105 64-65 2.1373603456806656 1105 66-67 2.416923502861021 1105 68-69 2.30949401634739 1105 70-71 2.1457355697851277 1105 72-73 2.0850607323940835 1105 74-75 2.7588725933921694 1105 76-77 1.9974094264505418 1105 78-79 2.501728022487697 1105 80-81 3.1460405763018784 1105 82-83 2.812873327168635 1105 84-85 2.8404441864184093 1105 86-87 3.022494884857103 1105 88-89 2.2920647136956482 1105 90-91 2.263243583728091 1105 92-93 2.6727872850897647 1105 94-95 1.3758304737405567 1105 96-97 0.8987117364417827 1105 98-99 2.042719670588692 1105 100-101 3.434108694163953 1106 1 -0.40710855670661417 1106 2 -0.6402014239509448 1106 3 -0.7136304073811139 1106 4 -1.0969274709341192 1106 5 -0.6039912715259703 1106 6 0.04542445559884101 1106 7 -0.18719481679713112 1106 8 -0.5128074498389736 1106 9 -0.6559268123037185 1106 10-11 -0.33878688287572345 1106 12-13 -0.28433572359364234 1106 14-15 -0.7770337326743757 1106 16-17 -0.6728542287412154 1106 18-19 -0.44776545328761586 1106 20-21 0.06166519612601462 1106 22-23 -0.7504805143178359 1106 24-25 -0.575430856442189 1106 26-27 -0.38349748866218647 1106 28-29 -1.071621668132721 1106 30-31 -0.7830607285141227 1106 32-33 -1.4329859032629386 1106 34-35 -0.853406478096197 1106 36-37 -1.1293657740415242 1106 38-39 -1.2031665472330175 1106 40-41 -1.678320412814422 1106 42-43 -1.5328665150138079 1106 44-45 -1.6894025993603137 1106 46-47 -0.6996829430170877 1106 48-49 -0.8232799829326396 1106 50-51 -1.2799811699654313 1106 52-53 -0.9676531260887131 1106 54-55 -2.109676219693668 1106 56-57 -2.8568880642805183 1106 58-59 -3.8595956534550737 1106 60-61 -3.0958171705527526 1106 62-63 -2.904281326971226 1106 64-65 -3.1468829493384938 1106 66-67 -4.0337469952232645 1106 68-69 -3.249174566028085 1106 70-71 -3.427779755885364 1106 72-73 -3.560293673736183 1106 74-75 -3.4595182112055305 1106 76-77 -3.1618338685686176 1106 78-79 -2.780839027320731 1106 80-81 -2.6774747493686206 1106 82-83 -1.911025140264318 1106 84-85 -2.4179370396352304 1106 86-87 -2.7090664178248893 1106 88-89 -3.7855214932009034 1106 90-91 -3.2717181020956616 1106 92-93 -2.233342983109466 1106 94-95 -3.627761480282434 1106 96-97 -3.54573270800266 1106 98-99 -4.42327649799368 1106 100-101 -3.394435367138726 1107 1 0.007107129567899051 1107 2 -0.0666720121862383 1107 3 -0.37866308711967633 1107 4 -0.0037902160321579004 1107 5 0.2211721271668381 1107 6 0.03398654710210991 1107 7 -0.10304448999973914 1107 8 -0.355944704740935 1107 9 -0.15020785805535297 1107 10-11 0.20696475111120805 1107 12-13 0.13927538751745772 1107 14-15 0.2103028686328159 1107 16-17 -0.01885096076735948 1107 18-19 0.11514304344440518 1107 20-21 -0.7581877450504564 1107 22-23 -0.2782582920956074 1107 24-25 -0.8282903335663718 1107 26-27 0.29460708650121603 1107 28-29 -0.39310859623729755 1107 30-31 0.25778894468849245 1107 32-33 -0.46362315816489996 1107 34-35 -0.049076412736717145 1107 36-37 0.1463695200761279 1107 38-39 0.01946417172123205 1107 40-41 -0.11867988993859768 1107 42-43 -0.5778011555366902 1107 44-45 0.7350255052148569 1107 46-47 -0.98767313909552 1107 48-49 -0.6831656038476766 1107 50-51 -1.0242622157170658 1107 52-53 -0.5134047600756446 1107 54-55 -1.0569801412622937 1107 56-57 -1.094633162319738 1107 58-59 -0.8142525162001739 1107 60-61 -1.0280067130364117 1107 62-63 -0.5333662942914863 1107 64-65 -0.8629777205803215 1107 66-67 -0.03456398868731725 1107 68-69 -0.21772031766207434 1107 70-71 -0.018057533663142777 1107 72-73 0.4082520778977994 1107 74-75 0.6336190436964344 1107 76-77 1.090208615091516 1107 78-79 1.5247165282348263 1107 80-81 0.9430317865790911 1107 82-83 0.9611153826114993 1107 84-85 1.4345956401033355 1107 86-87 0.7447734514561581 1107 88-89 0.778203996995174 1107 90-91 0.49870673450564595 1107 92-93 1.670251788132365 1107 94-95 1.755816951090118 1107 96-97 1.594790167814331 1107 98-99 1.3814620640978212 1107 100-101 1.7052378354756499 1108 1 0.22279340407769865 1108 2 0.10979857604905163 1108 3 0.32721926582149763 1108 4 0.2315039016149001 1108 5 0.1035250683433091 1108 6 -0.0640526685841678 1108 7 -0.005005274313468533 1108 8 0.05582000114142005 1108 9 0.26155684782700206 1108 10-11 0.501082398170027 1108 12-13 -0.24307755365900618 1108 14-15 -0.1426383078377782 1108 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-0.26225530031121735 2316 74-75 -0.45522267855947973 2316 76-77 -0.9865045443990859 2316 78-79 -0.7879982483974786 2316 80-81 -0.5589896405484609 2316 82-83 -0.7009020016617988 2316 84-85 -0.3315252412388965 2316 86-87 -0.5837084567710491 2316 88-89 -1.3499544828916257 2316 90-91 -0.9316551009501879 2316 92-93 -0.6563097643236695 2316 94-95 -0.8852786624130182 2316 96-97 -0.853865583145847 2316 98-99 -0.5393990638585144 2316 100-101 0.3372370269047984 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 2.0 6 3.0 7 12.0 8 40.0 9 63.0 10 131.0 11 231.0 12 369.0 13 616.0 14 790.0 15 1146.0 16 1804.0 17 2836.0 18 2498.0 19 1404.0 20 1039.0 21 1031.0 22 992.0 23 1047.0 24 1149.0 25 1162.0 26 1331.0 27 1436.0 28 1657.0 29 1912.0 30 2285.0 31 2571.0 32 3305.0 33 4430.0 34 6391.0 35 9580.0 36 14459.0 37 24201.0 38 22296.0 39 2178.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.81124290871583 21.96235172769469 16.32886367543407 17.897541688155407 2 15.176247218618716 23.507074803048138 40.89124477014408 20.425433208189073 3 18.564592479316833 30.294074691363328 31.42380948289934 19.717523346420503 4 11.626388542856898 20.10927928934097 38.848271892370214 29.41606027543192 5 11.30185242464859 41.18293982197477 33.16063511702237 14.354572636354263 6 27.46673081383861 40.15842060498802 17.502727686663974 14.872120894509402 7 24.864474780711173 37.94104760350175 21.30258851021057 15.89188910557651 8 27.04748322580091 34.31730513148739 19.24758803769792 19.38762360501379 9 28.3825462418062 17.464067560717876 21.151384462065824 33.0020017354101 10-11 23.604584231823296 30.285053995309237 26.081409634103387 20.028952138764076 12-13 26.11056459338365 28.331049414271416 27.486500534823683 18.071885457521255 14-15 23.250199743984055 28.289332382580607 28.221891940652412 20.23857593278293 16-17 18.556001340217698 32.352082062560676 28.73220560314092 20.359710994080704 18-19 18.4430278610641 28.224039725427197 35.39506353147364 17.93786888203507 20-21 20.36658390536001 27.291042019261337 35.49944587152811 16.842928203850548 22-23 19.76700730679519 27.834980691331936 34.899934277503576 17.498077724369303 24-25 18.96064399178687 28.04620314607514 35.85511903023222 17.138033831905773 26-27 18.74860392790254 28.27797728483307 35.668568188456845 17.30485059880754 28-29 18.120937984596022 28.22373140547344 36.36472978603676 17.29060082389378 30-31 18.416395329856787 28.193111624670316 35.863710169331355 17.526782876141546 32-33 18.347236660108766 29.184099519755325 34.8731518312013 17.59551198893461 34-35 18.285380458595004 28.68151788245604 35.67771200783512 17.355389651113843 36-37 19.07146968616569 28.417340355157688 34.88947499548965 17.621714963186967 38-39 18.21020799147759 28.89157123342984 35.53509909878951 17.363121676303063 40-41 18.25203182185261 29.370779566659223 35.138490352067905 17.238698259420264 42-43 18.593169943627114 29.368898666300012 34.15973463495119 17.878196755121685 44-45 18.507461404307595 29.46116375570237 33.723657419737286 18.30771742025275 46-47 18.870437031245974 30.270019501885752 32.81514446000395 18.04439900686432 48-49 19.034527788039416 30.56211823125628 32.11582573733451 18.28752824336979 50-51 19.29398018883324 30.7601439874913 31.15490682909647 18.79096899457899 52-53 19.008754370742018 30.853357846716893 31.31556113025026 18.822326652290826 54-55 19.07662436962517 30.73694791192364 30.89545442830265 19.290973290148543 56-57 19.433156642239194 30.9633244271858 30.784199176968873 18.81931975360613 58-59 19.12258696380553 30.371824500210483 31.065558982465486 19.4400295535185 60-61 18.79354633630873 30.797085885617577 31.212897018015617 19.196470760058077 62-63 19.356265947301953 30.217613553381046 31.837902387477556 18.58821811183945 64-65 19.03238000326463 30.078437099975087 31.734379161333 19.15480373542728 66-67 19.16640177321111 30.33617127294908 31.249838916141893 19.24758803769792 68-69 19.266918100670967 30.139434187578928 31.05610872945644 19.53753898229366 70-71 19.03280956021959 30.790212974338267 30.968908667600235 19.208068797841907 72-73 19.222673734310433 29.915205457091552 31.01444170482564 19.84767910377237 74-75 19.197329873967988 30.305672729147158 30.28333576748941 20.21366162939544 76-77 18.93487057448947 29.89716406498338 31.483517899638315 19.68444746088884 78-79 18.916399625426337 29.353774516963206 30.974063351059716 20.75576250655074 80-81 18.707205388362443 30.30782051392194 30.654043419617004 20.33093067809861 82-83 18.909526714147027 30.316411653021074 30.158334693597023 20.615726939234875 84-85 18.549987542848307 29.952576912172784 30.109365200731965 21.38807034424694 86-87 18.492426910884113 30.216324882516172 29.826287167415526 21.464961039184185 88-89 18.226531155765944 29.715461473036708 30.425948676535018 21.632058694662327 90-91 18.043048633785872 29.73620897061351 30.746691871455578 21.474050524145042 92-93 18.405543098189817 29.59139840373893 29.846559619234174 22.156498878837084 94-95 18.39234014037921 29.80953444617222 29.408757807197656 22.38936760625091 96-97 19.363568415536218 29.847335458208402 28.720607565357092 22.068488560898288 98-99 19.076174970896894 30.428750005369576 28.08847345066218 22.406601573071356 100-101 18.855367853030643 30.525958514495866 27.65041502689512 22.968258605578374 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 463.0 1 296.5 2 136.5 3 147.0 4 134.5 5 145.5 6 231.0 7 435.0 8 659.5 9 721.0 10 658.0 11 599.0 12 592.5 13 606.5 14 632.0 15 609.0 16 571.0 17 588.0 18 615.0 19 607.5 20 605.5 21 634.5 22 678.0 23 767.0 24 836.5 25 898.0 26 1036.0 27 1249.5 28 1424.0 29 1669.5 30 1993.5 31 2272.0 32 2616.5 33 3023.0 34 3444.0 35 3822.5 36 4481.5 37 4822.0 38 4886.0 39 4916.5 40 4590.0 41 4308.5 42 3952.5 43 3860.0 44 3937.5 45 3806.5 46 3473.0 47 3257.0 48 3615.5 49 3488.5 50 2952.5 51 2546.0 52 2197.5 53 2083.5 54 1942.0 55 1729.5 56 1525.0 57 1398.5 58 1241.5 59 1133.0 60 964.5 61 787.5 62 635.5 63 431.5 64 310.5 65 235.0 66 168.5 67 143.0 68 103.5 69 61.5 70 46.0 71 39.5 72 33.5 73 22.5 74 18.0 75 17.5 76 15.5 77 11.0 78 6.0 79 5.0 80 5.5 81 2.5 82 1.5 83 2.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050687720684885605 2 0.0 3 0.0 4 0.0 5 0.00945025300904647 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004725126504523235 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.2955695495665774E-4 24-25 0.0 26-27 8.591139099133155E-4 28-29 4.2955695495665774E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.591139099133155E-4 42-43 0.02663253120731278 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.016323164288352992 92-93 0.001718227819826631 94-95 0.0 96-97 0.0 98-99 0.0021477847747832885 100-101 0.018041392108179625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 116399.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.82865832180689 #Duplication Level Percentage of deduplicated Percentage of total 1 83.15123722762526 46.422220122165996 2 8.499015142188847 9.489772248902483 3 2.6914317370429646 4.507770685315166 4 1.4542041117813616 3.247450579472332 5 0.9294595592761296 2.5945240079382126 6 0.5786039640526899 1.9381609807644398 7 0.4662686199679921 1.822180602926142 8 0.30161270466576384 1.3470906107440785 9 0.26775821740736183 1.3453723829242519 >10 1.5296072879478027 16.100653785685445 >50 0.08617505847593254 3.135765771183601 >100 0.03847100824818417 3.2440141238326787 >500 0.001538840329927367 0.8247493535167828 >1k 0.004616520989782101 3.9802747446283906 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1921 1.650357820943479 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1548 1.3299083325458123 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1164 1.000008591139099 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 960 0.8247493535167828 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 457 0.39261505683038517 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 310 0.26632531207312776 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 188 0.16151341506370331 No Hit GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA 167 0.1434720229555237 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 166 0.14261290904561036 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 166 0.14261290904561036 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 151 0.12972620039691063 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 148 0.12714885866717068 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 138 0.11855771956803753 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 136 0.11683949174821089 No Hit ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT 135 0.11598037783829758 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 135 0.11598037783829758 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 134 0.11512126392838426 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 131 0.11254392219864431 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 126 0.10824835264907774 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 117 0.1005163274598579 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 8.591139099133154E-4 0.0 4 0.0 0.0 0.0 8.591139099133154E-4 0.0 5 0.0 0.0 0.0 8.591139099133154E-4 0.0 6 0.0 0.0 0.0 8.591139099133154E-4 0.0 7 0.0 0.0 0.0 8.591139099133154E-4 0.0 8 0.0 0.0 0.0 8.591139099133154E-4 0.0 9 0.0 0.0 0.0 8.591139099133154E-4 0.0 10-11 0.0 0.0 0.0 8.591139099133154E-4 0.0 12-13 0.0 0.0 0.0 8.591139099133154E-4 0.0 14-15 0.0 0.0 0.0 8.591139099133154E-4 0.0 16-17 0.0 0.0 0.0 0.001288670864869973 0.0 18-19 0.0 0.0 0.0 0.0017182278198266307 0.0 20-21 0.0 0.0 0.0 0.0017182278198266307 0.0 22-23 0.0 0.0 0.0 0.0025773417297399463 0.0 24-25 0.0 0.0 0.0 0.003006898684696604 0.0 26-27 0.0 0.0 0.0 0.006013797369393208 0.0 28-29 0.0 0.0 0.0 0.014604936468526362 0.0 30-31 0.0 0.0 0.0 0.02835075902713941 0.0 32-33 0.0 0.0 0.0 0.0416670246307958 0.0 34-35 0.0 0.0 0.0 0.05884930282906211 0.0 36-37 0.0 0.0 0.0 0.07216556843271849 0.0 38-39 0.0 0.0 0.0 0.0893478466309848 0.0 40-41 0.0 0.0 0.0 0.10610056787429445 0.0 42-43 0.0 0.0 0.0 0.13745822558613047 0.0 44-45 0.0 0.0 0.0 0.19287107277553933 0.0 46-47 0.0 0.0 0.0 0.2555863881992113 0.0 48-49 0.0 0.0 0.0 0.4007766389745616 0.0 50-51 0.0 0.0 0.0 0.5111727763984226 0.0 52-53 0.0 0.0 0.0 0.6366034072457667 0.0 54-55 0.0 0.0 0.0 0.8543887834087922 0.0 56-57 0.0 0.0 0.0 1.0803357417159942 0.0 58-59 0.0 0.0 0.0 1.3483792816089486 0.0 60-61 0.0 0.0 0.0 1.6632445295921787 0.0 62-63 0.0 0.0 0.0 2.140911863503982 0.0 64-65 0.0 0.0 0.0 2.444608630658339 0.0 66-67 0.0 0.0 0.0 2.869440459110473 0.0 68-69 0.0 0.0 0.0 3.3737403242295896 0.0 70-71 0.0 0.0 0.0 3.8118884182853803 0.0 72-73 0.0 0.0 0.0 4.449350939441061 0.0 74-75 0.0 0.0 0.0 5.185611560236772 0.0 76-77 0.0 0.0 0.0 5.803314461464446 0.0 78-79 0.0 0.0 0.0 6.361738502908101 0.0 80-81 0.0 0.0 0.0 7.07909861768572 0.0 82-83 0.0 0.0 0.0 7.7565099356523675 0.0 84-85 0.0 0.0 0.0 8.4360690383938 0.0 86-87 0.0 0.0 0.0 9.529291488758496 0.0 88-89 0.0 0.0 0.0 10.85490425175474 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGGTCG 65 6.6029315E-10 58.476353 9 GGGTATG 25 0.0050049424 57.014446 7 GGGCGTA 25 0.0050049424 57.014446 9 AGAGGGC 25 0.0050049424 57.014446 8 GTACCAC 25 0.0050134696 56.989944 6 CGTATAG 25 0.0050134696 56.989944 1 CCCTGGT 70 1.2751116E-9 54.29947 7 TAGATAT 60 8.2325394E-5 39.59337 7 GGTATCA 1130 0.0 39.506306 1 CCTGGTC 105 4.586036E-8 36.199646 8 ATAACCC 105 4.6034984E-8 36.18409 3 AGATATA 55 0.0025669506 34.55421 8 TGTAGTA 55 0.0025669506 34.55421 7 AGTGTGG 55 0.0025669506 34.55421 8 TGTAGAT 55 0.0025723716 34.53936 5 AATAACC 110 6.931441E-8 34.53936 2 CGTAGCT 75 3.0570404E-4 31.674694 8 TAGTAGC 60 0.0039316127 31.674694 9 GTTCTGA 75 3.0647655E-4 31.661081 1 GTCTAAG 30 0.0039479434 31.66108 72-73 >>END_MODULE