FastQCFastQC Report
Thu 26 May 2016
SRR937704_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937704_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74773
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16592.2187153116766747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12261.6396292779479225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9601.2838858946411138No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3600.4814572104904177No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.3865031495325853No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2810.375804100410576No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.21665574472068796No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.18188383507415778No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.1778716916534043No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.16182311797039037No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTTC156.595298E-494.9932868
ATCGTAT200.00206702771.2449658
TCGTATG200.00206702771.2449659
AGTATCG250.00497774957.0725825
CTGCACT250.00497774957.0725824
AGTTTGA250.0050043156.995976
GTATCGT250.0050043156.995976
GGTATCA4600.049.6283261
GGGATCT459.570463E-442.219247
TGGGATC704.2581214E-640.711416
GTATCAA8000.038.6428951
GTACATG5150.036.940181
ATGGGGA550.002548260434.5894435
ATGGGAC550.002548260434.5894435
TACATGG5500.033.7247052
CATGGGG851.5978838E-533.5721054
ACATGGG5350.032.8922963
ATGGGAG1051.8517749E-631.706995
GCCAAAA300.003941382731.6644336-37
CATGGGA4500.030.6500914