##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937704_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 74773 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04876091637356 33.0 31.0 34.0 31.0 34.0 2 32.111751568079384 33.0 31.0 34.0 31.0 34.0 3 32.11346341593891 34.0 31.0 34.0 30.0 34.0 4 35.73679001778717 37.0 35.0 37.0 35.0 37.0 5 35.75107324836505 37.0 35.0 37.0 35.0 37.0 6 35.85669961082209 37.0 36.0 37.0 35.0 37.0 7 35.80949005657122 37.0 36.0 37.0 35.0 37.0 8 35.82700974950851 37.0 36.0 37.0 35.0 37.0 9 37.38556698273441 39.0 38.0 39.0 35.0 39.0 10-11 37.3717117141214 39.0 37.0 39.0 35.0 39.0 12-13 37.285443943669506 39.0 37.0 39.0 35.0 39.0 14-15 38.32785229962687 40.0 38.0 41.0 34.0 41.0 16-17 38.298169125219 40.0 38.0 41.0 34.0 41.0 18-19 38.397790646356306 40.0 38.0 41.0 34.5 41.0 20-21 38.50866622978883 40.0 38.0 41.0 35.0 41.0 22-23 38.45230898853865 40.0 38.0 41.0 35.0 41.0 24-25 38.38904417369906 40.0 38.0 41.0 34.0 41.0 26-27 38.246278736977246 40.0 38.0 41.0 34.0 41.0 28-29 38.055220467280975 40.0 38.0 41.0 34.0 41.0 30-31 37.64463108341246 39.5 37.0 41.0 34.0 41.0 32-33 37.30654113115697 39.0 35.5 41.0 33.0 41.0 34-35 37.145881534778596 40.0 35.0 41.0 33.0 41.0 36-37 36.90992069329838 40.0 35.0 41.0 33.0 41.0 38-39 36.66097388094633 39.0 35.0 41.0 32.5 41.0 40-41 36.31155631043291 39.0 35.0 41.0 31.0 41.0 42-43 35.87263450710818 39.0 35.0 41.0 30.0 41.0 44-45 35.336284487716156 39.0 35.0 41.0 27.5 41.0 46-47 34.55927941904163 39.0 35.0 40.5 21.0 41.0 48-49 33.86501812151445 38.5 35.0 40.0 7.5 41.0 50-51 32.88353416340122 37.5 33.5 39.5 2.0 40.5 52-53 32.92727321359314 38.0 33.5 39.5 2.0 40.5 54-55 33.107625747261714 38.0 34.0 40.0 2.0 41.0 56-57 32.81837026734249 38.0 34.0 40.0 2.0 41.0 58-59 32.54547764567424 38.0 33.0 40.0 2.0 41.0 60-61 32.151445040322045 38.0 33.0 40.0 2.0 41.0 62-63 31.753754697551255 37.0 32.5 40.0 2.0 41.0 64-65 31.279539405935296 36.5 31.5 40.0 2.0 41.0 66-67 30.754670803632326 36.0 31.0 39.0 2.0 41.0 68-69 30.266707233894586 35.5 30.0 39.0 2.0 40.0 70-71 29.777071937731534 35.0 29.0 38.5 2.0 40.0 72-73 29.339741617963703 35.0 29.0 38.0 2.0 40.0 74-75 28.81506024902037 35.0 28.5 37.0 2.0 39.5 76-77 28.297473687026066 34.5 27.0 37.0 2.0 39.0 78-79 27.840450429968037 34.0 26.0 36.0 2.0 39.0 80-81 27.357956749093923 34.0 25.5 36.0 2.0 38.0 82-83 26.845692964037823 34.0 24.5 35.0 2.0 37.0 84-85 26.35240661736188 34.0 23.0 35.0 2.0 37.0 86-87 25.83952095007556 33.0 20.0 35.0 2.0 36.0 88-89 25.36800716836291 33.0 18.0 35.0 2.0 36.0 90-91 24.861373757907266 32.5 9.5 35.0 2.0 35.0 92-93 24.28135824428604 32.0 2.0 35.0 2.0 35.0 94-95 23.662110654915544 31.5 2.0 34.0 2.0 35.0 96-97 22.91995773875597 31.0 2.0 34.0 2.0 35.0 98-99 21.942980755085394 31.0 2.0 34.0 2.0 35.0 100-101 19.100751608200824 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.5440267780556525 1101 2 -0.408011296052738 1101 3 -0.4265262243004493 1101 4 -0.16113860200378127 1101 5 -0.39511151466343364 1101 6 -0.39613909557346716 1101 7 -0.4130077529045977 1101 8 -0.060642385649053665 1101 9 -0.3942344746684938 1101 10-11 0.11449555835984881 1101 12-13 -0.033126696477786766 1101 14-15 0.10758631225597526 1101 16-17 -0.301332636639998 1101 18-19 -0.033298668215294924 1101 20-21 0.08841356141915213 1101 22-23 0.08593927468695028 1101 24-25 -0.08548871252612855 1101 26-27 0.47974400699190056 1101 28-29 0.2627484159529061 1101 30-31 -0.015453181155905327 1101 32-33 0.2953564641583384 1101 34-35 0.7822284783445852 1101 36-37 0.4164869786046026 1101 38-39 0.15095349898432886 1101 40-41 0.807720957585083 1101 42-43 1.3470130489183347 1101 44-45 1.6515715874706842 1101 46-47 1.1858071934548988 1101 48-49 0.45617257546084744 1101 50-51 0.23828481738200225 1101 52-53 0.48343777857698456 1101 54-55 0.6519572303356682 1101 56-57 1.147205204733794 1101 58-59 1.1584217777125332 1101 60-61 1.0546699971408167 1101 62-63 1.0145211306838675 1101 64-65 0.8509864476302198 1101 66-67 1.2228371996933483 1101 68-69 1.361793671926531 1101 70-71 1.4976778040805598 1101 72-73 0.9717783950224401 1101 74-75 0.9241117377626828 1101 76-77 0.600282273339058 1101 78-79 1.0566330328596756 1101 80-81 1.723554746618774 1101 82-83 1.8167755087518351 1101 84-85 1.6975694244532562 1101 86-87 2.0232984794712507 1101 88-89 1.2584100815422552 1101 90-91 2.055686280176257 1101 92-93 1.4713337298697766 1101 94-95 2.067018310382341 1101 96-97 2.544299653013347 1101 98-99 2.8553737543931206 1101 100-101 2.997133466034338 1102 1 0.033335514823882306 1102 2 -0.44887560415842387 1102 3 -0.12155533312266087 1102 4 -0.3367991483539825 1102 5 0.09055798824744699 1102 6 -0.3125072102174471 1102 7 0.0902390003421587 1102 8 0.019966660477237497 1102 9 0.23742696733777535 1102 10-11 0.15519193095277473 1102 12-13 0.09937442309230704 1102 14-15 -0.04293988568400664 1102 16-17 0.06359123259422006 1102 18-19 -0.330992353840017 1102 20-21 -0.4011744367895318 1102 22-23 0.5223476938539875 1102 24-25 0.19412391622443437 1102 26-27 -0.04595012646085905 1102 28-29 -0.054817638682116865 1102 30-31 0.7631518345180837 1102 32-33 0.42808149774544546 1102 34-35 0.42245463150177187 1102 36-37 1.086381739016609 1102 38-39 0.7189114031491286 1102 40-41 0.7207863404780568 1102 42-43 1.0241850148834075 1102 44-45 1.7227762448822403 1102 46-47 2.1078851155328238 1102 48-49 1.5474735158997177 1102 50-51 1.7724876834814225 1102 52-53 2.330504504954064 1102 54-55 2.7191314354409 1102 56-57 2.721152808853809 1102 58-59 2.33285079696914 1102 60-61 2.525337260911531 1102 62-63 2.5415027697344676 1102 64-65 2.633174087576478 1102 66-67 2.9218967608218804 1102 68-69 2.2403762961988107 1102 70-71 2.030761100094889 1102 72-73 2.5959946959628795 1102 74-75 2.9528287104451714 1102 76-77 3.006294364695975 1102 78-79 1.8263934448614663 1102 80-81 2.6218418043885876 1102 82-83 2.4186563864947814 1102 84-85 2.1628067733112957 1102 86-87 1.9485671762916716 1102 88-89 2.258857909576289 1102 90-91 2.805966172697527 1102 92-93 0.9895827222566993 1102 94-95 0.9902717810496071 1102 96-97 1.5330479736582205 1102 98-99 2.8741265533183338 1102 100-101 2.5806533943818515 1103 1 0.08528356677193472 1103 2 -0.4953831506659725 1103 3 -0.23966695123427684 1103 4 -0.04301185456668577 1103 5 0.06388196157141834 1103 6 0.014625116914878333 1103 7 0.26942467952783744 1103 8 0.33218147269204934 1103 9 -0.0647843348735293 1103 10-11 0.2136334893943328 1103 12-13 -0.00575018203230826 1103 14-15 0.04130019855607259 1103 16-17 0.7931454621484448 1103 18-19 0.19340217055451348 1103 20-21 0.12918709357199987 1103 22-23 -0.1973785258722245 1103 24-25 0.10777782987834428 1103 26-27 0.33400775349702627 1103 28-29 0.21510163123715387 1103 30-31 0.5862476576139031 1103 32-33 0.771008840672792 1103 34-35 0.9643114233585592 1103 36-37 1.2518784045132705 1103 38-39 1.2229479071856346 1103 40-41 1.5044823741740956 1103 42-43 2.400018640717029 1103 44-45 1.8046470767530707 1103 46-47 1.3141855718332778 1103 48-49 1.2845742530004571 1103 50-51 1.5536389646327038 1103 52-53 1.236699661149217 1103 54-55 1.5198509861604563 1103 56-57 1.6531464908474902 1103 58-59 1.9058588699772159 1103 60-61 1.8778293634036238 1103 62-63 1.7682489964807004 1103 64-65 1.8345605389629327 1103 66-67 2.679267768192883 1103 68-69 2.003802059624576 1103 70-71 2.024355343689134 1103 72-73 1.35889395886214 1103 74-75 1.229680237296698 1103 76-77 1.75067835907997 1103 78-79 2.0527886712566925 1103 80-81 1.5570822396290254 1103 82-83 2.1809413987797903 1103 84-85 1.7682823787868998 1103 86-87 2.184878162602658 1103 88-89 1.8441509948693735 1103 90-91 2.2477878645192213 1103 92-93 1.7152759479499267 1103 94-95 0.6130341538119808 1103 96-97 1.1231673137775608 1103 98-99 1.732936662128445 1103 100-101 1.8188948826233364 1104 1 0.02179150327987145 1104 2 0.1745010192182015 1104 3 0.5537693422020169 1104 4 0.8450190334642045 1104 5 0.827931725621184 1104 6 0.8404509427407092 1104 7 0.5909605010636554 1104 8 0.9694616099721856 1104 9 0.5173692472800511 1104 10-11 0.5152207909816369 1104 12-13 0.359114682832562 1104 14-15 0.7918364490923295 1104 16-17 0.5996662686692531 1104 18-19 0.6545776317299712 1104 20-21 0.3737173381022387 1104 22-23 0.3232134947197949 1104 24-25 0.35574007784059347 1104 26-27 0.621438040927309 1104 28-29 0.4617768779123992 1104 30-31 0.4363120076782536 1104 32-33 0.02476259442654083 1104 34-35 0.3979236069707426 1104 36-37 0.15492430755917752 1104 38-39 -0.3092271249893983 1104 40-41 0.34993496962668047 1104 42-43 0.8726698633682517 1104 44-45 1.7032957254017163 1104 46-47 1.6540611617088672 1104 48-49 0.9291473975736011 1104 50-51 1.1310735420672842 1104 52-53 0.5094366838862356 1104 54-55 0.6426523589618256 1104 56-57 1.0811816688826639 1104 58-59 1.6272230913414347 1104 60-61 2.0368812724555347 1104 62-63 1.4404926687243709 1104 64-65 2.0148479692503614 1104 66-67 1.9702373091624246 1104 68-69 2.1393661951887104 1104 70-71 2.5062300755638667 1104 72-73 2.214320814288996 1104 74-75 2.1873164449329074 1104 76-77 2.2321240905257014 1104 78-79 2.6229302413982616 1104 80-81 2.798609481156266 1104 82-83 2.694991162829556 1104 84-85 2.553138663643182 1104 86-87 3.159245386969882 1104 88-89 3.1708348215532 1104 90-91 2.931507298238653 1104 92-93 2.962165694839676 1104 94-95 3.5970538878317164 1104 96-97 3.341850282460527 1104 98-99 2.9441121233039063 1104 100-101 3.7516490653775207 1105 1 -0.6123908518327177 1105 2 -0.7125421701505736 1105 3 -0.6654997607880198 1105 4 -0.8161105346421067 1105 5 -1.0731387801004857 1105 6 -0.9244069383962454 1105 7 -1.0351007761604123 1105 8 -0.6813320408214665 1105 9 -0.5638415316051422 1105 10-11 -1.204971161768455 1105 12-13 -0.6060015962372063 1105 14-15 -1.446443358954923 1105 16-17 -2.0289789878829794 1105 18-19 -1.4869875214630923 1105 20-21 -1.2469914105134876 1105 22-23 -2.2127776459225146 1105 24-25 -2.1643780469286966 1105 26-27 -1.6546786072823565 1105 28-29 -1.1934464517295353 1105 30-31 -2.198793197194391 1105 32-33 -1.5269169921367691 1105 34-35 -1.2495477846866905 1105 36-37 -1.003621281218976 1105 38-39 -0.605461897968361 1105 40-41 -0.5871266767372916 1105 42-43 -0.21924597273362423 1105 44-45 -0.1804252048308399 1105 46-47 0.4416211469432696 1105 48-49 -0.30876327056962793 1105 50-51 -0.02304236786258329 1105 52-53 -0.014557409875294525 1105 54-55 -0.20249345130026342 1105 56-57 0.8792621413817443 1105 58-59 0.22578344571574505 1105 60-61 0.42355532192028633 1105 62-63 1.2003671611570041 1105 64-65 0.8051765037649439 1105 66-67 0.47263671853857403 1105 68-69 0.723160953402072 1105 70-71 0.6228782852353341 1105 72-73 1.4406035754554765 1105 74-75 1.2323515132237866 1105 76-77 1.613814751286128 1105 78-79 1.741977860445882 1105 80-81 1.1959885878376966 1105 82-83 1.166013680363701 1105 84-85 1.9486720708044984 1105 86-87 2.6001830023261014 1105 88-89 2.2479850614941377 1105 90-91 2.589868317064788 1105 92-93 1.7325606745369768 1105 94-95 0.7838386792676708 1105 96-97 1.1550654910245761 1105 98-99 1.4420818538317768 1105 100-101 1.8203090875259171 1106 1 -0.24804976656139743 1106 2 -0.9313190866019063 1106 3 -0.9859131974805244 1106 4 0.7868179752631477 1106 5 -0.8440259463364868 1106 6 -0.8262157239259622 1106 7 -2.6259707158675596 1106 8 -1.7183690778585046 1106 9 -1.9376572077463976 1106 10-11 -1.0085887328278886 1106 12-13 -1.177922354204476 1106 14-15 -1.5653064080505317 1106 16-17 -1.8765242075212214 1106 18-19 -1.69198321483087 1106 20-21 -2.0945366301517296 1106 22-23 -2.2799611084548133 1106 24-25 -2.2130429909424763 1106 26-27 -1.3626889431996716 1106 28-29 -0.6572707411352141 1106 30-31 -2.2806192092529614 1106 32-33 -2.5122744426104973 1106 34-35 -1.832235123187985 1106 36-37 -1.3847582321233673 1106 38-39 -1.473248289010563 1106 40-41 -2.240012120320408 1106 42-43 -1.7093407186423306 1106 44-45 -0.6855931634871695 1106 46-47 -1.6660446583969524 1106 48-49 -2.060270591844386 1106 50-51 -2.069984658990922 1106 52-53 -1.4297167552672008 1106 54-55 -1.3282471119376424 1106 56-57 -1.1754320877310889 1106 58-59 -1.7457927816744352 1106 60-61 -0.8361346005603316 1106 62-63 -0.6256448974131956 1106 64-65 0.5360119904143872 1106 66-67 -6.62161737047029E-4 1106 68-69 0.5018000576225745 1106 70-71 -0.3297487604149687 1106 72-73 -0.907372307404124 1106 74-75 -1.5058052481887891 1106 76-77 -1.2811034227018148 1106 78-79 -3.4802443617763394 1106 80-81 -3.8363111537643704 1106 82-83 -2.3843739165355196 1106 84-85 -1.994480383975862 1106 86-87 -1.639696411971915 1106 88-89 -2.1783715276531517 1106 90-91 -3.1663762996449414 1106 92-93 -3.4478872152132354 1106 94-95 -4.1304593396815115 1106 96-97 -4.0973031566929095 1106 98-99 -3.346893167701385 1106 100-101 -1.7589329452044886 1107 1 -1.018164709090133 1107 2 -0.8304250891561864 1107 3 -0.3920434656797589 1107 4 -0.8594144640727421 1107 5 -0.44683565259446567 1107 6 -0.7151046128148479 1107 7 -0.42162844255977205 1107 8 -0.7158147994421569 1107 9 -0.13561378501332655 1107 10-11 -0.2863665106056672 1107 12-13 -0.2960577309605412 1107 14-15 -0.48293092912332725 1107 16-17 -0.47805677457103 1107 18-19 0.04428753868126023 1107 20-21 0.06255149245363611 1107 22-23 0.1721461712386798 1107 24-25 0.44468370126697465 1107 26-27 -0.39525599300809944 1107 28-29 -0.2932860668057202 1107 30-31 -0.21803938805245338 1107 32-33 -0.6227469841175193 1107 34-35 -1.972081866483002 1107 36-37 -2.9671337110505718 1107 38-39 -2.599046501015671 1107 40-41 -3.4552100768976715 1107 42-43 -4.286607640736836 1107 44-45 -5.727738757356903 1107 46-47 -6.607296254820966 1107 48-49 -6.220551562470185 1107 50-51 -6.97292207916972 1107 52-53 -6.7924242903885315 1107 54-55 -6.813560011043645 1107 56-57 -6.7622775538869 1107 58-59 -6.496750636080563 1107 60-61 -5.721192071824699 1107 62-63 -6.063065076212684 1107 64-65 -5.799875621335296 1107 66-67 -5.604749007203203 1107 68-69 -7.176999431521743 1107 70-71 -6.368701506264269 1107 72-73 -6.18770436359825 1107 74-75 -6.4853710208580075 1107 76-77 -5.240234968040252 1107 78-79 -5.654573863692047 1107 80-81 -5.901445253381226 1107 82-83 -5.097017594696442 1107 84-85 -5.444671954857089 1107 86-87 -4.489632555011507 1107 88-89 -4.310555435699126 1107 90-91 -4.293451650858227 1107 92-93 -4.778666270130223 1107 94-95 -3.876947206859036 1107 96-97 -3.9255279331935498 1107 98-99 -3.786867624917221 1107 100-101 -3.5545906718966993 1108 1 0.8852835667719319 1108 2 0.8649772096943913 1108 3 0.5251979136305849 1108 4 0.5831142715594382 1108 5 0.648566646256107 1108 6 0.45156205385181636 1108 7 0.003658913762066618 1108 8 0.6520012925118692 1108 9 0.8253057552165615 1108 10-11 0.43030015606100136 1108 12-13 0.9424480161658977 1108 14-15 0.7791380363939098 1108 16-17 0.21792023692322005 1108 18-19 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32-33 -0.7912096277956735 2311 34-35 -0.29751290096576355 2311 36-37 -0.3292026765678102 2311 38-39 -0.5473223630846391 2311 40-41 -0.8291324906907747 2311 42-43 -0.7672110890126973 2311 44-45 -1.182120941264948 2311 46-47 -1.7829428065451012 2311 48-49 -2.1864279992517943 2311 50-51 -1.4125772515835138 2311 52-53 -1.0118926811931281 2311 54-55 -1.0215341489746805 2311 56-57 -0.9971913469903484 2311 58-59 -1.9575983372299888 2311 60-61 -1.9923846005603316 2311 62-63 -1.9161541566724551 2311 64-65 -1.438525046622651 2311 66-67 -1.1557547543296423 2311 68-69 -1.1741258683033564 2311 70-71 -1.5820635752297854 2311 72-73 -1.6909371222189407 2311 74-75 -1.5949256185591558 2311 76-77 -1.0530941634425552 2311 78-79 -1.195522139554118 2311 80-81 -1.7911722648754775 2311 82-83 -1.6656239165355196 2311 84-85 -1.0743414950869763 2311 86-87 -1.672103819379327 2311 88-89 -1.8033715276531517 2311 90-91 -1.0714688922375366 2311 92-93 -1.7337668448428687 2311 94-95 -1.1003667470889233 2311 96-97 -0.8982290826188333 2311 98-99 -2.3619394639976825 2311 100-101 -2.441803315574859 2312 1 0.7431783036140374 2312 2 0.5053280868873706 2312 3 0.9673031767884837 2312 4 0.9690791838401438 2312 5 0.12575962871224533 2312 6 0.5480532819219945 2312 7 0.46857119446382 2312 8 0.7923521697048486 2312 9 0.7972355797779684 2312 10-11 0.5820545420259151 2312 12-13 0.4643778407273018 2312 14-15 0.28703277323602094 2312 16-17 -0.7829569560592375 2312 18-19 -0.5028993941681037 2312 20-21 -0.27728516816342363 2312 22-23 0.3940154997323191 2312 24-25 0.11346773030508217 2312 26-27 -0.37438484963242047 2312 28-29 -0.3400192791469152 2312 30-31 -1.4390012755297619 2312 32-33 -0.6009683997255095 2312 34-35 -0.7152760588605034 2312 36-37 -1.960781623936235 2312 38-39 -1.2052170999267489 2312 40-41 -0.6893298591118295 2312 42-43 -0.36918477322322474 2312 44-45 -0.48091480091407846 2312 46-47 -0.2484033328608959 2312 48-49 -0.3711867711816197 2312 50-51 -0.544156198951935 2312 52-53 -0.3468707513685665 2312 54-55 -0.5494946752904681 2312 56-57 -0.10574397856929352 2312 58-59 -0.9899448284580608 2312 60-61 -2.3010468812620886 2312 62-63 -1.4287638057952599 2312 64-65 -2.0185689062717778 2312 66-67 -1.9178161578384092 2312 68-69 -1.1302662191805481 2312 70-71 -1.065616206808734 2312 72-73 -0.8537660695873619 2312 74-75 -1.3805615834714366 2312 76-77 -1.425352935372377 2312 78-79 -0.49486424481727553 2312 80-81 -1.122860861366707 2312 82-83 -0.6782335656583278 2312 84-85 -1.061731845964168 2312 86-87 -1.1742968018354638 2312 88-89 -0.6345118785303434 2312 90-91 -0.548442576448064 2312 92-93 0.5584480674378369 2312 94-95 0.4138876388759911 2312 96-97 0.7223849524688859 2312 98-99 -0.08178595522575094 2312 100-101 -0.6117594559257391 2313 1 0.29956928105765 2313 2 0.3649772096943913 2313 3 -0.3509925625598882 2313 4 -0.3930762046310363 2313 5 -0.32286192517246803 2313 6 -0.572247469957702 2313 7 0.05127796138111762 2313 8 -0.6099034693928971 2313 9 -1.9746942447834357 2313 10-11 -0.9173188915580468 2313 12-13 -0.3551710314531533 2313 14-15 -0.382766725510848 2313 16-17 -0.25350833450534793 2313 18-19 -0.2779620508097054 2313 20-21 0.07609829048318772 2313 22-23 -0.7125007909944969 2313 24-25 -0.11648214438162796 2313 26-27 -0.13253021304094403 2313 28-29 -0.26044534430982225 2313 30-31 -0.5636879923217464 2313 32-33 0.1358737055376551 2313 34-35 0.723320432367565 2313 36-37 -0.043488390853525516 2313 38-39 0.09553477977249969 2313 40-41 0.46104608073779474 2313 42-43 0.7179079586063537 2313 44-45 0.23901001111600806 2313 46-47 1.0310452886929937 2313 48-49 -1.5629160944898892 2313 50-51 -2.2578153468216087 2313 52-53 -1.974690300240745 2313 54-55 -1.162903196593728 2313 56-57 -2.7680246803236805 2313 58-59 -1.8727769086585617 2313 60-61 -2.0385155529412877 2313 62-63 -1.4444279661962618 2313 64-65 -1.751025046622651 2313 66-67 -0.5694452305201168 2313 68-69 -0.6741258683033564 2313 70-71 0.1530554723892621 2313 72-73 -0.5555204555522764 2313 74-75 -0.6261756185591594 2313 76-77 0.4186320270336381 2313 78-79 -0.07945071098269096 2313 80-81 0.24305392560071226 2313 82-83 0.24657846441686004 2313 84-85 -0.3317819712774508 2313 86-87 0.548134275858768 2313 88-89 0.4406760913944687 2313 90-91 0.22912634585770064 2313 92-93 1.303435536109518 2313 94-95 1.0097523005301277 2313 96-97 0.4053423459525938 2313 98-99 0.6425248217166057 2313 100-101 0.4302204939489478 2314 1 -0.6803414332280653 2314 2 -0.2808561236389444 2314 3 -0.2341770863694137 2314 4 -0.3023023951072261 2314 5 -0.22539168707722723 2314 6 0.17552038718515206 2314 7 -0.7098827529045977 2314 8 -0.4157070408214665 2314 9 -0.7142775781167714 2314 10-11 -0.9504290106056672 2314 12-13 -0.711197817167438 2314 14-15 -0.12086196360608881 2314 16-17 -0.27062142974344283 2314 18-19 -0.5082447889049462 2314 20-21 -0.5745713523739511 2314 22-23 -0.6072180528992561 2314 24-25 -0.6838184539054382 2314 26-27 -0.545848665421893 2314 28-29 -0.5904304633574426 2314 30-31 -0.19352430184555658 2314 32-33 -0.2313137944623449 2314 34-35 -0.25063790096576355 2314 36-37 0.3270473234321898 2314 38-39 0.08549013691536089 2314 40-41 -0.24319499069077466 2314 42-43 -0.3843985890126973 2314 44-45 0.30485822540171625 2314 46-47 1.0608071934548988 2314 48-49 1.4828428340815378 2314 50-51 1.4155477484164862 2314 52-53 1.6938364854735397 2314 54-55 1.0644033510253195 2314 56-57 1.0731211530096516 2314 58-59 1.1778183294366755 2314 60-61 1.2914695661063327 2314 62-63 1.1723875099942127 2314 64-65 1.0953291200440134 2314 66-67 0.9432035790036934 2314 68-69 0.8779574650299793 2314 70-71 0.7773114247702146 2314 72-73 1.0200003777810593 2314 74-75 1.3139285481075085 2314 76-77 1.1864891698907805 2314 78-79 1.101352860445882 2314 80-81 -0.46044309820881324 2314 82-83 1.1416677501311447 2314 84-85 1.4230543382463594 2314 86-87 1.5831045139540088 2314 88-89 2.240899305680184 2314 90-91 2.1629061077624634 2314 92-93 1.107378988490467 2314 94-95 1.7720290862444124 2314 96-97 2.018437584047831 2314 98-99 2.7969147026689853 2314 100-101 3.0087175177584733 2315 1 0.675447501198164 2315 2 0.1955783025905653 2315 3 0.27273889723714717 2315 4 0.36726727702392026 2315 5 0.5791677391522825 2315 6 -0.6167439570771407 2315 7 0.43535290283310957 2315 8 0.20391386081787743 2315 9 -0.09491282401840806 2315 10-11 0.09887939103367671 2315 12-13 0.34272124020960604 2315 14-15 0.2397937740988283 2315 16-17 -0.22169724941556979 2315 18-19 0.5618115635540732 2315 20-21 0.6258640984457244 2315 22-23 -0.052274405358275544 2315 24-25 -0.01207152767592845 2315 26-27 -0.0111406736186197 2315 28-29 -0.20736181581646207 2315 30-31 0.2661324604495263 2315 32-33 -0.011667278068905773 2315 34-35 -0.3149309337526489 2315 36-37 -1.1734649716497785 2315 38-39 -1.0145354778387414 2315 40-41 -0.5857923267563478 2315 42-43 -0.6534815808159706 2315 44-45 -0.9852288647622203 2315 46-47 -1.445340347528706 2315 48-49 -1.8228692560823987 2315 50-51 -3.0642165958458065 2315 52-53 -1.8856973260018712 2315 54-55 -0.988234968646811 2315 56-57 -1.098625773219858 2315 58-59 -1.3068128181043086 2315 60-61 -0.9727466224182599 2315 62-63 -1.2838112604975898 2315 64-65 -0.6376370684805792 2315 66-67 -0.8992656832913895 2315 68-69 -0.4022679448060842 2315 70-71 -1.2839078375248683 2315 72-73 -2.4844822041861505 2315 74-75 -2.283279443695772 2315 76-77 -1.9671993546993853 2315 78-79 -1.65146476250494 2315 80-81 -1.6578047785366792 2315 82-83 -1.5783629875737724 2315 84-85 -1.6083237355241309 2315 86-87 -2.8285245844066473 2315 88-89 -3.3368414730083416 2315 90-91 -2.185198400434256 2315 92-93 -1.4245113803619915 2315 94-95 -1.9996153809687023 2315 96-97 -2.2008247110341372 2315 98-99 -2.0257031251998683 2315 100-101 -1.0664617855202145 2316 1 -0.24284143322806528 2316 2 -0.4058561236389444 2316 3 0.2814479136305863 2316 4 -0.3491773951072261 2316 5 -0.03789168707722723 2316 6 -0.43385461281484794 2316 7 -0.4442577529045977 2316 8 -0.3532070408214665 2316 9 -0.1205275781167714 2316 10-11 -0.0988665106056672 2316 12-13 -0.258072817167438 2316 14-15 0.05882553639391119 2316 16-17 -0.22374642974344283 2316 18-19 -0.3129322889049462 2316 20-21 -0.5589463523739511 2316 22-23 -0.16190555289925612 2316 24-25 -0.34788095390543816 2316 26-27 -0.33491116542189303 2316 28-29 -0.5513679633574426 2316 30-31 -0.7403993018455566 2316 32-33 -0.3719387944623449 2316 34-35 -0.8678254009657635 2316 36-37 -1.0792026765678102 2316 38-39 -1.805134863084639 2316 40-41 -1.4306949906907747 2316 42-43 -2.0797110890126973 2316 44-45 -2.4685792745982837 2316 46-47 -2.845442806545101 2316 48-49 -2.587469665918462 2316 50-51 -2.9360147515835138 2316 52-53 -3.0327260145264603 2316 54-55 -2.8965341489746805 2316 56-57 -3.0440663469903484 2316 58-59 -2.9315566705633245 2316 60-61 -2.5991554338936673 2316 62-63 -2.3432374900057873 2316 64-65 -2.3031083799559866 2316 66-67 -2.9083589209963066 2316 68-69 -3.4736050349700207 2316 70-71 -2.4258135752297854 2316 72-73 -2.4331246222189407 2316 74-75 -2.3345089518924915 2316 76-77 -2.5400733301092195 2316 78-79 -1.547084639554118 2316 80-81 -1.3041930982088132 2316 82-83 -1.1708322498688553 2316 84-85 -1.1550706617536406 2316 86-87 -1.2450204860459912 2316 88-89 -0.899725694319816 2316 90-91 -2.0480313922375366 2316 92-93 -2.525433511509533 2316 94-95 -2.2904709137555876 2316 96-97 -1.083124915952169 2316 98-99 -1.8749602973310147 2316 100-101 -2.6865949822415267 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 344.0 3 69.0 4 65.0 5 48.0 6 50.0 7 61.0 8 89.0 9 106.0 10 131.0 11 204.0 12 342.0 13 538.0 14 606.0 15 856.0 16 1374.0 17 1944.0 18 1693.0 19 898.0 20 833.0 21 750.0 22 832.0 23 851.0 24 860.0 25 874.0 26 937.0 27 963.0 28 1077.0 29 1164.0 30 1523.0 31 1809.0 32 2393.0 33 3096.0 34 4422.0 35 7064.0 36 9537.0 37 13978.0 38 11251.0 39 1141.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.02203782892532 20.46274078741002 16.348744621241572 19.166476762423088 2 14.489180679305955 22.118309104307627 42.24157566825216 21.150934548134252 3 17.07983812612226 29.80998579583523 32.30509473909897 20.80508133894353 4 10.612556606554302 19.041239047134166 39.62994721187599 30.716257134435544 5 10.356008736081893 39.65002076829285 34.73262497822679 15.26134551739847 6 25.865948726191434 40.0934929947226 18.65171850304053 15.388839776045435 7 23.733944093971417 37.14656915926655 22.304817776349097 16.81466897041293 8 24.961159327118825 34.44765884495875 20.621718632808314 19.969463195114113 9 27.216715778194484 16.572461826948835 22.118939190999196 34.091883203857485 10-11 22.769405933962897 29.130667738262677 27.115397495144332 20.984528832630097 12-13 25.288164654034972 27.825219179274917 28.53851460413778 18.348101562552323 14-15 22.255858655539853 27.583735742654497 29.74502541708805 20.4153801847176 16-17 17.407667838607065 31.503766951280767 30.291645739159552 20.79691947095262 18-19 17.248699215846464 27.387114703381034 37.1009756718207 18.263210408951807 20-21 18.375287113861155 26.33411682771829 37.79724236762628 17.493353690794276 22-23 18.11710185303001 26.67439041540821 37.55750955981329 17.65099817174849 24-25 17.511786135219115 27.053198328511733 37.81139504982321 17.623620486445944 26-27 17.184224801269732 27.136475961506264 38.074509620085315 17.604789617138685 28-29 16.749263327082776 27.074069113313687 38.545405839807124 17.63126171979641 30-31 16.90474755389468 27.194128007822176 38.40368606808243 17.49743837020071 32-33 16.77794370743402 27.882432513878936 37.50828718182244 17.8313365968646 34-35 16.97317135241565 27.566669791987568 37.47237439558526 17.987784460011518 36-37 17.14804224229721 27.472895418840277 37.09393352932116 18.285128809541348 38-39 16.747468795200085 28.004633845824184 37.23415653291905 18.01374082605668 40-41 16.617784920316055 28.0520958885764 37.608142493638674 17.721976697468865 42-43 16.82012683064025 27.83443713044002 36.93624316125705 18.409192877662672 44-45 16.906284528074465 28.184953292931997 36.403388355040676 18.50537382395286 46-47 17.286868943903894 28.657430038906334 35.52463286748408 18.53106814970569 48-49 17.34806629834254 28.893353423740166 34.635861376192864 19.122718901724426 50-51 17.585213955668653 29.08189914953459 34.05142971941338 19.28145717538338 52-53 17.53876033888089 29.439105247295984 33.80839165522553 19.213742758597597 54-55 17.475273051141425 29.00871218969939 33.76994729828368 19.746067460875505 56-57 17.543389757874436 29.170907435183203 33.477073066209556 19.808629740732805 58-59 17.61228206432952 28.40662578001553 33.96448753314229 20.016604622512656 60-61 17.99073243246863 28.44553964831456 33.87081653698322 19.69291138223359 62-63 17.9263981144204 28.013177630169274 34.80755838868652 19.252865866723806 64-65 18.00936125191678 27.30833874607437 35.02768868145628 19.65461132055257 66-67 17.993237135491647 27.79470353878603 34.17523184572634 20.036827479995985 68-69 17.630909334404716 27.49229945091737 34.50381679389313 20.372974420784786 70-71 17.841214994575914 27.563046593541994 34.51725661939009 20.078481792492 72-73 17.677745113139444 27.160832792022983 34.19027784288383 20.971144251953742 74-75 17.565477107288032 27.402947892879386 33.982468006455626 21.049106993376952 76-77 17.33062793391861 26.922072443096205 34.71014056023197 21.037159062753215 78-79 17.32736915412225 26.242834949375904 34.69826431670863 21.731531579793216 80-81 17.267887635182642 26.598587069340745 34.650952556333074 21.482572739143535 82-83 17.366965535251538 26.748180338955812 34.348236586068126 21.536617539724524 84-85 17.021447559611893 26.353111335801955 33.655861417828994 22.969579686757154 86-87 17.1210863218791 26.312371106409195 33.91541902134612 22.65112355036559 88-89 17.37881435946885 25.724368374883653 34.224606092261126 22.672211173386366 90-91 17.077449464007124 26.01758297678592 34.3149267822349 22.59004077697206 92-93 17.27208811811577 26.039706719341122 34.116307877732766 22.571897284810337 94-95 16.81888793657596 25.921362759802875 34.12524105421041 23.134508249410757 96-97 17.309726928176353 26.524394326962998 33.209230068703214 22.956648676157442 98-99 17.67955431158013 26.731261132166438 32.11621646957989 23.47296808667354 100-101 18.047232284545387 25.999852692052738 32.44792329273437 23.504991730667506 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 150.0 1 109.0 2 97.5 3 126.0 4 95.0 5 76.0 6 155.5 7 404.5 8 685.5 9 733.5 10 561.0 11 394.5 12 355.5 13 363.0 14 357.5 15 349.5 16 352.5 17 354.0 18 341.0 19 356.0 20 401.0 21 443.0 22 485.0 23 550.0 24 628.0 25 685.0 26 790.0 27 929.0 28 1000.5 29 1112.0 30 1320.0 31 1462.5 32 1672.5 33 2024.0 34 2336.0 35 2544.5 36 2940.5 37 3347.0 38 3243.0 39 2980.0 40 2882.5 41 2772.0 42 2546.0 43 2454.0 44 2497.5 45 2452.5 46 2243.5 47 2135.0 48 2356.5 49 2247.5 50 1820.5 51 1521.5 52 1291.0 53 1205.5 54 1133.0 55 1013.0 56 899.5 57 767.0 58 674.0 59 571.5 60 474.0 61 404.5 62 313.5 63 231.0 64 160.5 65 115.0 66 84.0 67 67.0 68 49.5 69 29.5 70 21.5 71 19.5 72 17.5 73 13.0 74 11.5 75 10.0 76 8.0 77 8.0 78 4.0 79 3.0 80 4.0 81 2.5 82 1.5 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23270431840370187 2 0.1845585973546601 3 0.19659502761692052 4 0.18054645393390661 5 0.1872333596351624 6 0.15379883112888343 7 0.14978668770812995 8 0.14577454428737646 9 0.15112406884838112 10-11 0.15781097454963688 12-13 0.15981704626001364 14-15 0.15847966511976247 16-17 0.1477806159977532 18-19 0.14243109143674856 20-21 0.1437684725769997 22-23 0.14911799713800436 24-25 0.14577454428737646 26-27 0.14911799713800436 28-29 0.15112406884838112 30-31 0.1517927594185067 32-33 0.14644323485750202 34-35 0.15246144998863226 36-37 0.14510585371725088 38-39 0.14042501972637184 40-41 0.1377502574458695 42-43 0.14243109143674856 44-45 0.1410937102964974 46-47 0.14243109143674856 48-49 0.1477806159977532 50-51 0.1444371631471253 52-53 0.154467521699009 54-55 0.1437684725769997 56-57 0.13507549516536715 58-59 0.12705120832386022 60-61 0.13908763858612067 62-63 0.13507549516536715 64-65 0.13841894801599508 66-67 0.1344068045952416 68-69 0.1377502574458695 70-71 0.14176240086662298 72-73 0.14510585371725088 74-75 0.14644323485750202 76-77 0.1437684725769997 78-79 0.14042501972637184 80-81 0.1410937102964974 82-83 0.1370815668757439 84-85 0.1370815668757439 86-87 0.13240073288486484 88-89 0.13975632915624625 90-91 0.13173204231473928 92-93 0.1344068045952416 94-95 0.13507549516536715 96-97 0.13908763858612067 98-99 0.13641287630561832 100-101 0.13306942345499043 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 74773.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.188223021678944 #Duplication Level Percentage of deduplicated Percentage of total 1 85.58924731182796 53.22643200085593 2 6.989247311827956 8.69297741163254 3 2.5827956989247314 4.81858424832493 4 1.3053763440860215 3.247161408529817 5 0.821505376344086 2.554397977879716 6 0.5118279569892473 1.9097802682786567 7 0.42365591397849467 1.8442485924063496 8 0.30752688172043013 1.5299640244473274 9 0.23225806451612901 1.2999344683241276 >10 1.1827956989247312 12.520562235031363 >50 0.03225806451612903 1.2317280301713185 >100 0.015053763440860216 1.9819988498522192 >500 0.002150537634408602 1.2838858946411138 >1k 0.004301075268817204 3.8583445896245974 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1659 2.2187153116766747 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1226 1.6396292779479225 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 960 1.2838858946411138 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 360 0.4814572104904177 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 289 0.3865031495325853 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 281 0.375804100410576 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 162 0.21665574472068796 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 136 0.18188383507415778 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 133 0.1778716916534043 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 121 0.16182311797039037 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.686905701255801E-4 0.0 20-21 0.0 0.0 0.0 0.0013373811402511602 0.0 22-23 0.0 0.0 0.0 0.0013373811402511602 0.0 24-25 0.0 0.0 0.0 0.0026747622805023204 0.0 26-27 0.0 0.0 0.0 0.003343452850627901 0.0 28-29 0.0 0.0 0.0 0.010699049122009282 0.0 30-31 0.0 0.0 0.0 0.021398098244018563 0.0 32-33 0.0 0.0 0.0 0.03343452850627901 0.0 34-35 0.0 0.0 0.0 0.042796196488037126 0.0 36-37 0.0 0.0 0.0 0.06486298530218126 0.0 38-39 0.0 0.0 0.0 0.07823679670469287 0.0 40-41 0.0 0.0 0.0 0.09161060810720448 0.0 42-43 0.0 0.0 0.0 0.1377502574458695 0.0 44-45 0.0 0.0 0.0 0.2113062201596833 0.0 46-47 0.0 0.0 0.0 0.2741631337514878 0.0 48-49 0.0 0.0 0.0 0.46942078022815725 0.0 50-51 0.0 0.0 0.0 0.6038275848233988 0.0 52-53 0.0 0.0 0.0 0.7556203442419055 0.0 54-55 0.0 0.0 0.0 1.0404825271154026 0.0 56-57 0.0 0.0 0.0 1.3788399555989461 0.0 58-59 0.0 0.0 0.0 1.7767108448236664 0.0 60-61 0.0 0.0 0.0 2.1692322094873817 0.0 62-63 0.0 0.0 0.0 2.759017292338143 0.0 64-65 0.0 0.0 0.0 3.1615690155537424 0.0 66-67 0.0 0.0 0.0 3.6891658753828254 0.0 68-69 0.0 0.0 0.0 4.319072392441122 0.0 70-71 0.0 0.0 0.0 4.839982346568949 0.0 72-73 0.0 0.0 0.0 5.72800342369572 0.0 74-75 0.0 0.0 0.0 6.781191071643508 0.0 76-77 0.0 0.0 0.0 7.613042140879729 0.0 78-79 0.0 0.0 0.0 8.357963435999626 0.0 80-81 0.0 0.0 0.0 9.237960226284889 0.0 82-83 0.0 0.0 0.0 10.083185106923622 0.0 84-85 0.0 0.0 0.0 10.908349270458588 0.0 86-87 0.0 0.0 0.0 12.39217364556725 0.0 88-89 0.0 0.0 0.0 14.069918286012332 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGTTC 15 6.595298E-4 94.993286 8 ATCGTAT 20 0.002067027 71.244965 8 TCGTATG 20 0.002067027 71.244965 9 AGTATCG 25 0.004977749 57.072582 5 CTGCACT 25 0.004977749 57.072582 4 AGTTTGA 25 0.00500431 56.99597 6 GTATCGT 25 0.00500431 56.99597 6 GGTATCA 460 0.0 49.628326 1 GGGATCT 45 9.570463E-4 42.21924 7 TGGGATC 70 4.2581214E-6 40.71141 6 GTATCAA 800 0.0 38.642895 1 GTACATG 515 0.0 36.94018 1 ATGGGGA 55 0.0025482604 34.589443 5 ATGGGAC 55 0.0025482604 34.589443 5 TACATGG 550 0.0 33.724705 2 CATGGGG 85 1.5978838E-5 33.572105 4 ACATGGG 535 0.0 32.892296 3 ATGGGAG 105 1.8517749E-6 31.70699 5 GCCAAAA 30 0.0039413827 31.66443 36-37 CATGGGA 450 0.0 30.650091 4 >>END_MODULE