FastQCFastQC Report
Thu 26 May 2016
SRR937704_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937704_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74773
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11361.519264975325318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10511.4055875784039693No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8551.143460874914742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7210.9642518021210865No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.3182967113797761No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.2527650355074693No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA1260.1685100236716462No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.16048573683013923No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1190.15914835568988805No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA1120.14978668770812995No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1050.14042501972637184No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA990.13240073288486484No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.12437644604335789No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG920.12303906490310675No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT910.12170168376285558No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.11635215920185094No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG860.11501477806159979No Hit
CAATAGATGCACAAGAAGTTGAAACCATTTGAACTATTCTACCAGCTGTA820.10966525350059513No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.10966525350059513No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG800.10699049122009281No Hit
GCTAAGGGTAGTTTATTTTGGACACAGACATATTAGGGCATTGTCACCAC790.10565311007984166No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATACA156.580239E-495.0481951
GTCATAG352.7641276E-454.313251
ACGCTCA205.359631E-447.52409440-41
CGTAGCT554.903991E-543.203728
AGGCTGC459.5434504E-442.243648
CTAGATC459.5749705E-442.2153854
ATGGGTA459.5749705E-442.2153855
TAGATCA459.5749705E-442.2153855
TCGTAGC704.241403E-640.734947
ATATTCG704.260988E-640.7076873
AACGCTC250.001607309138.0192838-39
CCCTGCA500.001607445437.9938472
TTCCGAG250.001612604737.99384770-71
TATTCGT756.843511E-637.9938434
CATGGGT651.3175943E-436.5325434
TATATTC801.0653854E-535.6192322
CCTAGAT550.002566327834.539863
GTAGCTT702.02902E-433.945789
GGTATCA8500.032.9873121
AACTTGC600.003917813331.6827327
Produced by FastQC (version 0.11.5)