FastQCFastQC Report
Thu 26 May 2016
SRR937703_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937703_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614186
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35370.5758841784084952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27110.4413972314575715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17890.2912798403089618No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7860.12797426186855448No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6770.11022719501909845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21100.041.9137271
GTATCAA33750.036.0655021
GGACCGA1452.395791E-829.4832136
ATCAACG42300.028.8786333
TCAACGC42400.028.812884
TATCAAC42900.028.4724142
CAACGCA42850.028.3993595
AACGCAG44150.027.5428966
AGGACCG1754.9112714E-927.1632275
GTACATG32450.026.0814111
CCCTATA1858.894858E-925.6907482
TAAGGTA1701.1129305E-725.165934
CCGAGTG1701.12087946E-725.1474489
TACATGG33400.024.7600352
ACATGGG33050.024.736653
ACGCAGA50700.023.9845927
CGCAGAG50400.023.9388628
TAGCGCG500.00165316723.75133528-29
ACCGAGT2002.0498192E-823.7503668
GCAGCGT1000.001652619323.7503666
Produced by FastQC (version 0.11.5)