##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937703_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 614186 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.936768340535277 33.0 31.0 34.0 30.0 34.0 2 32.075679028828404 33.0 31.0 34.0 30.0 34.0 3 32.09708785286542 34.0 31.0 34.0 30.0 34.0 4 35.68829475110146 37.0 35.0 37.0 35.0 37.0 5 35.67755533340063 37.0 35.0 37.0 35.0 37.0 6 35.75246586538931 37.0 35.0 37.0 35.0 37.0 7 35.69847733422774 37.0 35.0 37.0 35.0 37.0 8 35.68455158535036 37.0 35.0 37.0 35.0 37.0 9 37.34850680412774 39.0 37.0 39.0 35.0 39.0 10-11 37.228055507614954 39.0 37.0 39.0 34.0 39.0 12-13 37.12587880544331 39.0 37.0 39.0 34.0 39.0 14-15 38.29863266176696 40.0 38.0 41.0 33.5 41.0 16-17 38.22738470105148 40.0 38.0 41.0 33.0 41.0 18-19 38.184158381988524 40.0 38.0 41.0 33.5 41.0 20-21 38.088853376664396 40.0 38.0 41.0 33.5 41.0 22-23 38.00117228331483 40.0 38.0 41.0 33.0 41.0 24-25 38.030299453260085 40.0 38.0 41.0 33.0 41.0 26-27 37.93294376622066 40.0 38.0 41.0 33.0 41.0 28-29 37.79865057165093 40.0 38.0 41.0 33.0 41.0 30-31 37.52884549631545 40.0 37.0 41.0 32.5 41.0 32-33 37.35474677052228 40.0 37.0 41.0 32.5 41.0 34-35 37.362773654886304 40.0 37.0 41.0 32.5 41.0 36-37 37.21090597962181 40.0 36.5 41.0 32.0 41.0 38-39 37.045800457841764 40.0 36.0 41.0 31.0 41.0 40-41 36.786550002767896 39.5 36.0 41.0 31.0 41.0 42-43 36.49186402816085 39.0 35.5 41.0 30.0 41.0 44-45 36.196963949031726 39.0 35.0 41.0 30.0 41.0 46-47 35.768399963528964 39.0 35.0 41.0 28.5 41.0 48-49 35.37969523890157 39.0 35.0 40.0 26.5 41.0 50-51 34.46562849039216 38.0 34.0 39.5 25.0 40.5 52-53 34.540858143949876 38.0 34.0 39.5 25.5 40.5 54-55 34.80546202616146 38.0 35.0 40.0 25.5 41.0 56-57 34.554615377100745 38.0 34.0 40.0 24.0 41.0 58-59 34.29652173771463 38.0 34.0 40.0 23.0 41.0 60-61 33.95362642587099 38.0 34.0 40.0 22.5 41.0 62-63 33.5571536961116 37.0 33.0 40.0 21.5 41.0 64-65 33.15731472225026 36.5 33.0 39.5 19.5 41.0 66-67 32.71297050079292 36.0 32.5 39.0 18.0 41.0 68-69 32.20572758089569 35.5 32.0 39.0 14.5 40.0 70-71 31.743325311876205 35.0 31.0 38.0 9.5 40.0 72-73 31.284636901524948 35.0 31.0 37.5 7.5 40.0 74-75 30.75481450244714 35.0 30.5 37.0 4.5 39.0 76-77 30.278230861660802 34.5 30.0 36.0 2.0 39.0 78-79 29.725597783081998 34.0 29.0 36.0 2.0 38.5 80-81 29.181313152693157 34.0 29.0 35.0 2.0 37.0 82-83 28.69596262370031 34.0 29.0 35.0 2.0 37.0 84-85 28.19663750069197 34.0 27.5 35.0 2.0 36.0 86-87 27.68430166106033 33.0 26.5 35.0 2.0 36.0 88-89 27.203930079812956 33.0 26.0 35.0 2.0 35.5 90-91 26.670327067044838 33.0 25.0 35.0 2.0 35.0 92-93 26.067192186080437 32.0 24.0 35.0 2.0 35.0 94-95 25.339168427805255 32.0 21.0 34.0 2.0 35.0 96-97 24.50973809236941 31.5 12.5 34.0 2.0 35.0 98-99 23.47293083854077 31.0 2.0 34.0 2.0 35.0 100-101 20.69532275239097 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.05796429760609456 1101 2 0.22912327531084742 1101 3 0.21034117036188604 1101 4 0.004743086307385624 1101 5 0.038844243507945464 1101 6 0.20684163552726176 1101 7 0.2568761879146493 1101 8 0.14652433300557988 1101 9 0.20329688361246667 1101 10-11 0.3440065470053 1101 12-13 0.26164706390748194 1101 14-15 0.4617182607525052 1101 16-17 0.5057712655451354 1101 18-19 0.5414321593009035 1101 20-21 0.588789671862223 1101 22-23 0.3791490758166205 1101 24-25 0.2517368354265912 1101 26-27 0.13291219478436744 1101 28-29 0.09735427444105227 1101 30-31 0.24462592184935517 1101 32-33 0.06534697512222465 1101 34-35 0.3754956497072399 1101 36-37 0.41856021618524153 1101 38-39 0.4682555617365409 1101 40-41 0.4479779729806168 1101 42-43 0.6357179390428982 1101 44-45 0.7570677717762777 1101 46-47 0.9119937230059278 1101 48-49 1.1083012953074345 1101 50-51 1.2188347930930448 1101 52-53 0.9612215406803202 1101 54-55 1.0072183717746626 1101 56-57 0.9492331329422683 1101 58-59 1.120695909926681 1101 60-61 1.3127560273759613 1101 62-63 1.3104587055749803 1101 64-65 1.2136496565176387 1101 66-67 1.2381355071709024 1101 68-69 1.111774039084061 1101 70-71 1.2620397941535906 1101 72-73 1.318379471717197 1101 74-75 1.3976680167424966 1101 76-77 1.5257666229326112 1101 78-79 1.6681120735213177 1101 80-81 1.479023223850941 1101 82-83 1.7097232822654647 1101 84-85 1.552751148003889 1101 86-87 1.4731281583881994 1101 88-89 1.4313024359868223 1101 90-91 1.0714237086993883 1101 92-93 1.26253073578399 1101 94-95 1.356063655959293 1101 96-97 1.3323113091187402 1101 98-99 1.4761117098851813 1101 100-101 1.8827594561376877 1102 1 -0.0057161910007828 1102 2 0.11336256205228779 1102 3 0.15802116269243527 1102 4 -0.04184880325257723 1102 5 0.023193778547728527 1102 6 0.0194674623894997 1102 7 0.06499621475771278 1102 8 -0.05105979123946014 1102 9 0.14901158018997762 1102 10-11 -0.016876877402715706 1102 12-13 0.23210771005837927 1102 14-15 0.2154451325768747 1102 16-17 0.27638290393071685 1102 18-19 0.13381304320464693 1102 20-21 0.18180568183250756 1102 22-23 0.09182044001948242 1102 24-25 0.2570694976833252 1102 26-27 0.028667635298219807 1102 28-29 0.09961915796132814 1102 30-31 0.1633657358652627 1102 32-33 0.2058416544434749 1102 34-35 0.2339224544239471 1102 36-37 0.4246238725940188 1102 38-39 0.3365807462825927 1102 40-41 0.2583628833439633 1102 42-43 0.20963223297444955 1102 44-45 0.29783567514124343 1102 46-47 0.5316850277705711 1102 48-49 0.6700523258893512 1102 50-51 0.4899497389275709 1102 52-53 0.5832855805614443 1102 54-55 0.5195374207632497 1102 56-57 0.5003428060320942 1102 58-59 0.6364182759510371 1102 60-61 0.5195794383624346 1102 62-63 0.5957501445549482 1102 64-65 0.5970500975108308 1102 66-67 0.5810196985235976 1102 68-69 0.553318473941701 1102 70-71 0.7078743258380022 1102 72-73 0.8244790786580474 1102 74-75 0.6046232463465593 1102 76-77 0.6272909254922858 1102 78-79 0.9998276329115967 1102 80-81 0.8726695664834772 1102 82-83 0.8658804100576241 1102 84-85 0.8918245829573905 1102 86-87 0.8716637733819681 1102 88-89 0.872607200630668 1102 90-91 1.1998869432885932 1102 92-93 1.0671875280378522 1102 94-95 1.2604831747015055 1102 96-97 1.9038049338910525 1102 98-99 1.8842940509838293 1102 100-101 2.2090464851857163 1103 1 0.19208783163651333 1103 2 0.3694315013053284 1103 3 0.27736515929152716 1103 4 0.3217694447480568 1103 5 0.29183456642491024 1103 6 0.1773838744308449 1103 7 0.2128648900860668 1103 8 0.19630539018661608 1103 9 0.025533796803060227 1103 10-11 0.1868167396939313 1103 12-13 0.28383871943744765 1103 14-15 0.2799151576408647 1103 16-17 0.3837805445503193 1103 18-19 0.23459706779038925 1103 20-21 0.1737816629149549 1103 22-23 0.2598299785055431 1103 24-25 0.3374325712545385 1103 26-27 0.33665578525703665 1103 28-29 0.20933499609932937 1103 30-31 0.18694846916848462 1103 32-33 0.25664826318841705 1103 34-35 0.40521998079427846 1103 36-37 0.45724248371251264 1103 38-39 0.36411792157996103 1103 40-41 0.2737134692036207 1103 42-43 0.2855338280360442 1103 44-45 0.47950088047996076 1103 46-47 0.4292094053777191 1103 48-49 0.3926402491209231 1103 50-51 0.3759606236066517 1103 52-53 0.3411678949506509 1103 54-55 0.34858197798088497 1103 56-57 0.25267982368316666 1103 58-59 0.40401952826480425 1103 60-61 0.6678699060759428 1103 62-63 0.4360214644440603 1103 64-65 0.6950620272608106 1103 66-67 0.6047983826199257 1103 68-69 0.6627564383561477 1103 70-71 0.4329099733834063 1103 72-73 0.7297905629244248 1103 74-75 0.9822536366539723 1103 76-77 0.7912596707872517 1103 78-79 0.6160740570863901 1103 80-81 0.31058416293554814 1103 82-83 0.5079380926571915 1103 84-85 0.5827005826385552 1103 86-87 0.7181213815866592 1103 88-89 0.7153262448336264 1103 90-91 0.6732487060929273 1103 92-93 0.484806272004338 1103 94-95 0.8655061574046954 1103 96-97 1.1689086438927347 1103 98-99 1.1931601047784142 1103 100-101 0.7547738722427759 1104 1 0.2422915503471188 1104 2 0.2658557314511967 1104 3 0.2891971645139293 1104 4 0.20741121496235593 1104 5 0.23941076397578342 1104 6 0.18792716184304936 1104 7 0.27515104171582294 1104 8 0.1281581049354017 1104 9 0.20183489530837306 1104 10-11 0.15824265811640004 1104 12-13 0.29467886215309846 1104 14-15 0.4169905560741398 1104 16-17 0.4685362509252542 1104 18-19 0.40944202772195837 1104 20-21 0.32672826835345603 1104 22-23 0.3810679354657438 1104 24-25 0.4667404903973491 1104 26-27 0.3869326626206302 1104 28-29 0.48606041479193607 1104 30-31 0.29186641892537324 1104 32-33 0.4874047236602337 1104 34-35 0.4786571994148403 1104 36-37 0.2827323653080427 1104 38-39 0.5296590705084654 1104 40-41 0.60409091157711 1104 42-43 0.4807928659434779 1104 44-45 0.5228755203142867 1104 46-47 0.5185361206667523 1104 48-49 0.6342059005705849 1104 50-51 0.6719141059585354 1104 52-53 0.5692259266452382 1104 54-55 0.5483824799617949 1104 56-57 0.7737945364510423 1104 58-59 0.6260869917980258 1104 60-61 0.7081324893642602 1104 62-63 0.44587537224164464 1104 64-65 0.7775202705527349 1104 66-67 0.6138208141884505 1104 68-69 0.7776183373296774 1104 70-71 0.6912247356740657 1104 72-73 0.7334470886762681 1104 74-75 0.7733533237600412 1104 76-77 0.8786997369676968 1104 78-79 0.7458715764452926 1104 80-81 0.9890743934415802 1104 82-83 0.8168139255403197 1104 84-85 0.7505307532670464 1104 86-87 0.9691990648209483 1104 88-89 1.0509570412499798 1104 90-91 1.2787519250736565 1104 92-93 1.1029912620997813 1104 94-95 1.2248502056668968 1104 96-97 0.9087001980076295 1104 98-99 1.0021076894173433 1104 100-101 1.579990815786811 1105 1 0.33895108043056155 1105 2 0.3371254942970481 1105 3 0.2434063761959706 1105 4 0.29224048593293617 1105 5 0.19057320358930951 1105 6 0.21845664142144727 1105 7 0.32263676854937273 1105 8 0.26248369782077674 1105 9 0.30240582196625354 1105 10-11 0.4562704907909776 1105 12-13 0.3761011008443589 1105 14-15 0.3843715672730852 1105 16-17 0.5047166692388174 1105 18-19 0.3276698219687759 1105 20-21 0.42584442996961513 1105 22-23 0.6560253904503739 1105 24-25 0.5553507780565496 1105 26-27 0.47841816986700536 1105 28-29 0.44476430148495183 1105 30-31 0.25462580627715425 1105 32-33 0.21250216546962974 1105 34-35 0.5334510619532651 1105 36-37 0.5112780119922817 1105 38-39 0.4228299093314831 1105 40-41 0.80268349502024 1105 42-43 0.9804669162289485 1105 44-45 0.8618975339286905 1105 46-47 1.27318862396055 1105 48-49 0.9275983851994809 1105 50-51 1.108443118914174 1105 52-53 1.0479122463641701 1105 54-55 0.95414183986518 1105 56-57 1.1727844354409385 1105 58-59 1.0605608060272758 1105 60-61 1.2227830712702783 1105 62-63 0.8981837824304932 1105 64-65 0.7634872775795216 1105 66-67 0.989805525431315 1105 68-69 1.0539070395694665 1105 70-71 1.0918135037900036 1105 72-73 1.259183391926161 1105 74-75 1.0427833577960506 1105 76-77 1.0886696809729663 1105 78-79 1.0073655926947467 1105 80-81 0.8962850873370591 1105 82-83 0.9582555153514747 1105 84-85 0.9430297925731495 1105 86-87 0.8065192778205095 1105 88-89 1.46843000457892 1105 90-91 1.3443879044324625 1105 92-93 1.4174812708832683 1105 94-95 1.6981978121666756 1105 96-97 1.528896728530249 1105 98-99 1.3862514366725023 1105 100-101 1.949875120793397 1106 1 0.0490757383813083 1106 2 0.18620829555376162 1106 3 -0.13657740813564345 1106 4 -0.023731947430803757 1106 5 -0.05078688559686384 1106 6 -0.10638373559284275 1106 7 -0.008395966831187707 1106 8 0.05131515621745564 1106 9 0.08986908334255617 1106 10-11 -0.2160361880374424 1106 12-13 -0.1495652617785268 1106 14-15 0.0507645945356856 1106 16-17 -0.07926022343372097 1106 18-19 0.14943935678179088 1106 20-21 0.024912029037217565 1106 22-23 0.2937014824742974 1106 24-25 0.13225865279051163 1106 26-27 0.15968907287703615 1106 28-29 0.31698990197141796 1106 30-31 0.20813244456639524 1106 32-33 0.560454937335443 1106 34-35 0.40785057548321646 1106 36-37 0.15045405334223716 1106 38-39 0.4172659081152972 1106 40-41 0.6363158047395032 1106 42-43 0.7763724599605766 1106 44-45 0.7617911186048829 1106 46-47 0.9885681719487991 1106 48-49 1.1459981014252847 1106 50-51 1.0206454093773374 1106 52-53 0.9121212257905356 1106 54-55 0.7418787406455536 1106 56-57 0.6361876988442035 1106 58-59 0.7495645559005908 1106 60-61 0.6368450962018599 1106 62-63 0.7331082782245559 1106 64-65 0.6967243303817909 1106 66-67 0.5701642970944292 1106 68-69 0.3738523116886512 1106 70-71 0.27832409464841845 1106 72-73 0.45931514423182307 1106 74-75 0.3918762938455096 1106 76-77 0.754140442095899 1106 78-79 0.7157166619153834 1106 80-81 0.5012004618176498 1106 82-83 0.7887156349420295 1106 84-85 0.9013453900191806 1106 86-87 0.9301232101200938 1106 88-89 0.9224180455234823 1106 90-91 0.7945906002873322 1106 92-93 0.7221686125271312 1106 94-95 1.0241023424190345 1106 96-97 1.5171403689477998 1106 98-99 1.8941616424087968 1106 100-101 2.012482802966293 1107 1 0.2651351175985823 1107 2 0.22693029285470345 1107 3 0.20522421129755486 1107 4 0.24816413893896794 1107 5 0.2946921967243199 1107 6 0.2046486530711249 1107 7 0.280268000780147 1107 8 0.33950678914592913 1107 9 0.34145477834930915 1107 10-11 0.42441590373044136 1107 12-13 0.3040447247261966 1107 14-15 0.4631802490565988 1107 16-17 0.30145840004805535 1107 18-19 0.5692099370786821 1107 20-21 0.5478539993476019 1107 22-23 0.49354966061194006 1107 24-25 0.33909063659618255 1107 26-27 0.48232739946272574 1107 28-29 0.45700339724807293 1107 30-31 0.49608791015344167 1107 32-33 0.33946978213976564 1107 34-35 0.43141670233881513 1107 36-37 0.6689257132612667 1107 38-39 0.7255655032570019 1107 40-41 0.750975049004019 1107 42-43 0.7793582899200899 1107 44-45 0.9785589998464488 1107 46-47 1.0870668224211357 1107 48-49 1.45077205554135 1107 50-51 1.1197850854906974 1107 52-53 1.4334437629025487 1107 54-55 1.3990312372717355 1107 56-57 1.748940735281451 1107 58-59 1.5117777812717108 1107 60-61 1.7535455010601666 1107 62-63 1.8513943780896014 1107 64-65 1.7940590132427872 1107 66-67 1.5718343375802633 1107 68-69 1.635896846101602 1107 70-71 1.6772444725161613 1107 72-73 1.7474730389686677 1107 74-75 1.5877264962746587 1107 76-77 1.7373894299501522 1107 78-79 2.055173477030092 1107 80-81 2.0594325805760825 1107 82-83 1.843860709166048 1107 84-85 2.0169324345535955 1107 86-87 1.8188883923063308 1107 88-89 2.0449720266300986 1107 90-91 2.021716106360209 1107 92-93 2.3345336597605986 1107 94-95 2.6528472816902884 1107 96-97 2.746053999177221 1107 98-99 2.8982608326921984 1107 100-101 2.6316629649096157 1108 1 0.27309828793577395 1108 2 0.17033268180580308 1108 3 0.27697050966759207 1108 4 0.2847605057424971 1108 5 0.4392112958906793 1108 6 0.3685935330014445 1108 7 0.28431179821700425 1108 8 0.05889835316235548 1108 9 0.44533371327527504 1108 10-11 0.407540825965107 1108 12-13 0.4909148255100675 1108 14-15 0.5325158220315913 1108 16-17 0.38481506298198553 1108 18-19 0.3361005679110818 1108 20-21 0.283731814452743 1108 22-23 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-1.3642117550270996 2316 100-101 -2.0668190800465247 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2072.0 3 376.0 4 354.0 5 395.0 6 463.0 7 564.0 8 667.0 9 801.0 10 1097.0 11 1561.0 12 2166.0 13 2929.0 14 3396.0 15 4412.0 16 5936.0 17 8028.0 18 6667.0 19 4289.0 20 4115.0 21 4262.0 22 4638.0 23 5201.0 24 5825.0 25 6498.0 26 7368.0 27 8740.0 28 10466.0 29 12447.0 30 15354.0 31 19555.0 32 24779.0 33 32818.0 34 44688.0 35 63822.0 36 91412.0 37 117309.0 38 80644.0 39 8071.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.839381064195976 18.955725450109895 15.072190670894797 22.13270281479933 2 15.080704664024394 20.14286553793315 41.08461583665794 23.69181396138452 3 18.846543728401873 25.260075316962965 30.25247669401953 25.640904260615628 4 12.366938570959837 16.640575390185653 37.52067033087221 33.4718157079823 5 13.182877115994627 37.87297746911615 34.339639891141275 14.604505523747955 6 31.18189766543566 36.41802925708917 17.61509702339784 14.784976054077331 7 27.877941639439456 33.67941655751316 20.864658894610727 17.577982908436653 8 26.53199048653728 34.38124973492152 19.920525393702714 19.16623438483849 9 26.755166524477474 16.55555247433662 20.76397192320805 35.92530907797786 10-11 24.678815047548667 26.91420195806386 27.166973761161234 21.240009233226235 12-13 25.8424602064526 24.355437098995957 28.066674224253163 21.735428470298277 14-15 22.81585862556621 25.576323631837955 26.654019870585106 24.95379787201073 16-17 21.256736068460558 28.957407085687443 27.55681053530487 22.229046310547133 18-19 21.41628531558416 26.86247627684988 30.77048550594027 20.950752901625695 20-21 22.171115781369906 25.86627456065451 30.843851609730557 21.118758048245024 22-23 21.867346913810806 25.74503291005455 31.067482941492603 21.320137234642043 24-25 21.53950920345466 26.0203723790339 31.17389921462766 21.266219202883775 26-27 21.134783583763177 26.277069868906604 31.006218271524393 21.581928275805826 28-29 21.184820516835824 26.448605143563693 31.023117653391797 21.343456686208686 30-31 21.248133191628543 26.29966974518422 30.779520449453234 21.672676613733998 32-33 20.909974037795685 26.34058529558822 30.82569271288314 21.923747953732953 34-35 21.635430243730557 26.401805508202926 30.585729829546842 21.37703441851967 36-37 21.611593465424207 26.00220015542981 30.645278350649974 21.740928028496008 38-39 21.18630454441303 26.51930953559427 30.538266402362108 21.756119517630587 40-41 21.395690950768284 26.457898251900225 30.76295173378847 21.38345906354302 42-43 21.314652008677356 26.49415257140061 30.26814986380466 21.923045556117373 44-45 21.291124993883244 26.575513399774902 30.20911152070726 21.924250085634593 46-47 21.685326086513264 26.88261407502318 29.566406030172065 21.865653808291484 48-49 21.5807523702722 27.133285757977365 29.134386787644 22.151575084106433 50-51 21.855326034739956 27.31116459679254 28.873051617307503 21.960457751159996 52-53 21.90444027333544 27.165176732390183 28.930502091128673 21.99988090314571 54-55 21.72274228917716 27.34378758977713 28.93190597856358 22.001564142482128 56-57 21.76410679587109 27.550826540289 28.57506297003287 22.11000369380704 58-59 21.65569934032959 27.09459624746202 28.914030838980082 22.335673573228306 60-61 21.610188839242035 27.36155050389746 29.05539610580216 21.972864551058347 62-63 21.709062294868083 27.254646938667342 29.284255246280615 21.75203552018396 64-65 21.570231086612633 27.272052707969795 29.114385427029145 22.04333077838843 66-67 21.485339767272528 27.50566081735924 28.80033072056477 22.208668694803464 68-69 21.836504697925278 27.165848749802247 28.96037302880405 22.03727352346842 70-71 21.761675353734354 27.221399542053454 28.760527225639787 22.256397878572407 72-73 21.586746858274164 27.487695654584655 28.54599982387418 22.379557663267 74-75 21.544105535595758 27.766615512323522 28.453855901313972 22.235423050766745 76-77 21.529745042492916 27.339475276477142 28.747017504374874 22.38376217665507 78-79 21.460392587947382 27.452614548310205 28.760316829939175 22.32667603380324 80-81 21.702681105058875 27.669118366548158 28.38008415147265 22.24811637692032 82-83 21.775242400595573 27.448447828933798 28.32998608097497 22.446323689495664 84-85 21.403708749880753 27.604771817218953 28.389964213816942 22.601555219083355 86-87 21.671138767200464 27.583117066302048 28.301914483001532 22.443829683495952 88-89 21.7230886144483 27.30334128605838 28.076978899458094 22.896591200035225 90-91 21.37203273803942 27.329837296477898 28.470547751400304 22.82758221408238 92-93 21.62131530367964 27.15667664690189 28.579594006640445 22.64241404277803 94-95 21.305910575695258 26.72528372165841 28.9172669437971 23.05153875884923 96-97 21.575660520588805 26.56237387059398 28.718243928053955 23.143721680763264 98-99 21.223633439990085 27.089510129129685 28.473927806099987 23.212928624780243 100-101 21.655633458340127 26.23984999184738 29.128322191423443 22.976194358389044 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 754.0 1 438.0 2 150.0 3 175.0 4 157.5 5 171.0 6 279.0 7 545.5 8 938.5 9 1137.0 10 1003.0 11 845.5 12 864.0 13 920.0 14 965.0 15 1009.0 16 1087.5 17 1143.0 18 1212.5 19 1329.0 20 1471.0 21 1659.5 22 1917.0 23 2256.0 24 2671.5 25 3263.0 26 3918.0 27 4698.0 28 5358.0 29 6207.5 30 7505.0 31 9204.0 32 11344.0 33 12691.5 34 13309.0 35 14394.0 36 16445.0 37 18313.0 38 19277.5 39 19717.5 40 19695.0 41 19923.0 42 19887.0 43 20146.0 44 20779.0 45 21543.5 46 21804.5 47 21854.0 48 22551.5 49 22091.0 50 20885.0 51 19844.0 52 18848.5 53 18475.0 54 18631.5 55 17722.0 56 16198.0 57 14808.5 58 13629.0 59 12151.0 60 9940.5 61 8225.0 62 6782.5 63 4935.0 64 3270.5 65 2334.5 66 1760.0 67 1330.5 68 1010.0 69 761.0 70 553.5 71 368.5 72 277.0 73 210.5 74 150.5 75 113.0 76 91.5 77 68.0 78 46.5 79 36.0 80 22.0 81 14.0 82 14.5 83 10.5 84 7.0 85 4.5 86 3.5 87 2.5 88 2.0 89 2.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2898144861654287 2 0.25757669500770125 3 0.2564369751182866 4 0.25936768340535277 5 0.26848544252066964 6 0.1852858905934033 7 0.1847974392122256 8 0.18886786738870634 9 0.18772814749929173 10-11 0.19253125274753902 12-13 0.18162250523457063 14-15 0.18219236517927792 16-17 0.1891120930792952 18-19 0.1830878593781037 20-21 0.176900808549853 22-23 0.17510982015220145 24-25 0.1792616568922118 26-27 0.174784185898083 28-29 0.1869140618639956 30-31 0.19122871573106517 32-33 0.19204280136636132 34-35 0.19098449004047632 36-37 0.17055094059454304 38-39 0.16354980413099615 40-41 0.16843431794277303 42-43 0.17844757125691565 44-45 0.18170391379810025 46-47 0.18487884777575522 48-49 0.18292504225104447 50-51 0.18650701904634753 52-53 0.2018118289899151 54-55 0.1742957345169053 56-57 0.16241008424158154 58-59 0.16297994418628883 60-61 0.1572813447392158 62-63 0.16501515827452923 64-65 0.1621658585509927 66-67 0.15947937595451542 68-69 0.16981826352277649 70-71 0.16509656683805882 72-73 0.16086332153451888 74-75 0.16428248120276268 76-77 0.16688755523571036 78-79 0.17087657484866148 80-81 0.16379402982158497 82-83 0.1622472671145223 84-85 0.15638585054039003 86-87 0.15296669087214623 88-89 0.1593979673909858 90-91 0.16534079252864767 92-93 0.15858388175568966 94-95 0.16232867567805193 96-97 0.15899092457333772 98-99 0.16346839556746653 100-101 0.14425597457447745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 614186.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.36434558698475 #Duplication Level Percentage of deduplicated Percentage of total 1 80.76539704585038 49.561157357915995 2 11.231286556881596 13.784010993258763 3 3.4564738844770613 6.363127738783139 4 1.5023988358344436 3.6877488548651347 5 0.7787689172873629 2.389432248641184 6 0.4666970887603607 1.7183136863478283 7 0.31572033139616207 1.3561780067242009 8 0.23142892641524623 1.1361187695496024 9 0.1757882638474587 0.9708418595584362 >10 0.9756663905529714 10.963970843593092 >50 0.06380142601477962 2.6701528036149487 >100 0.035237576015027014 3.84272391849805 >500 5.339026668931939E-4 0.23965832947205698 >1k 8.008540003397908E-4 1.3165645891776636 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3537 0.5758841784084952 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2711 0.4413972314575715 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1789 0.2912798403089618 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 786 0.12797426186855448 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 677 0.11022719501909845 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.6281712705922962E-4 5 0.0 0.0 0.0 0.0 1.6281712705922962E-4 6 0.0 0.0 0.0 0.0 1.6281712705922962E-4 7 1.6281712705922962E-4 0.0 0.0 0.0 1.6281712705922962E-4 8 1.6281712705922962E-4 0.0 0.0 0.0 1.6281712705922962E-4 9 1.6281712705922962E-4 0.0 0.0 0.0 1.6281712705922962E-4 10-11 1.6281712705922962E-4 0.0 0.0 0.0 1.6281712705922962E-4 12-13 1.6281712705922962E-4 0.0 0.0 0.0 1.6281712705922962E-4 14-15 1.6281712705922962E-4 0.0 0.0 0.0 1.6281712705922962E-4 16-17 1.6281712705922962E-4 0.0 0.0 0.0 3.2563425411845923E-4 18-19 1.6281712705922962E-4 0.0 0.0 0.0 3.2563425411845923E-4 20-21 1.6281712705922962E-4 0.0 0.0 0.0 3.2563425411845923E-4 22-23 1.6281712705922962E-4 0.0 0.0 1.6281712705922962E-4 3.2563425411845923E-4 24-25 3.2563425411845923E-4 0.0 0.0 3.2563425411845923E-4 3.2563425411845923E-4 26-27 3.2563425411845923E-4 0.0 0.0 4.884513811776888E-4 3.2563425411845923E-4 28-29 3.2563425411845923E-4 0.0 0.0 0.001628171270592296 3.2563425411845923E-4 30-31 3.2563425411845923E-4 0.0 0.0 0.00390761104942151 3.2563425411845923E-4 32-33 3.2563425411845923E-4 0.0 0.0 0.00643127651883957 3.2563425411845923E-4 34-35 3.2563425411845923E-4 0.0 0.0 0.008222264916491097 3.2563425411845923E-4 36-37 3.2563425411845923E-4 0.0 0.0 0.01033888756826108 3.2563425411845923E-4 38-39 3.2563425411845923E-4 0.0 0.0 0.013513821545916058 3.2563425411845923E-4 40-41 3.2563425411845923E-4 0.0 0.0 0.020026506628285243 3.2563425411845923E-4 42-43 3.2563425411845923E-4 0.0 0.0 0.02727186878242096 3.2563425411845923E-4 44-45 3.2563425411845923E-4 0.0 0.0 0.044204849996580836 3.2563425411845923E-4 46-47 4.07042817648074E-4 0.0 0.0 0.08165278922020365 3.2563425411845923E-4 48-49 4.884513811776888E-4 0.0 0.0 0.1072150781685027 3.2563425411845923E-4 50-51 4.884513811776888E-4 0.0 0.0 0.13953427788975978 3.2563425411845923E-4 52-53 4.884513811776888E-4 0.0 0.0 0.1764937657322049 3.2563425411845923E-4 54-55 4.884513811776888E-4 0.0 0.0 0.2222453784358484 3.2563425411845923E-4 56-57 4.884513811776888E-4 0.0 0.0 0.2798012328512861 3.2563425411845923E-4 58-59 4.884513811776888E-4 0.0 0.0 0.35095231737616944 4.07042817648074E-4 60-61 4.884513811776888E-4 0.0 0.0 0.44790991653994067 4.884513811776888E-4 62-63 4.884513811776888E-4 0.0 0.0 0.5637543024425825 4.884513811776888E-4 64-65 4.884513811776888E-4 0.0 0.0 0.6527338623804515 4.884513811776888E-4 66-67 6.512685082369185E-4 0.0 0.0 0.7843715096078387 4.884513811776888E-4 68-69 6.512685082369185E-4 8.140856352961481E-5 0.0 0.9470258195400091 4.884513811776888E-4 70-71 6.512685082369185E-4 1.6281712705922962E-4 0.0 1.0819198093085807 4.884513811776888E-4 72-73 6.512685082369185E-4 1.6281712705922962E-4 0.0 1.2864181208949732 4.884513811776888E-4 74-75 6.512685082369185E-4 1.6281712705922962E-4 0.0 1.465679777787185 4.884513811776888E-4 76-77 6.512685082369185E-4 1.6281712705922962E-4 0.0 1.6521867968335324 4.884513811776888E-4 78-79 7.326770717665332E-4 1.6281712705922962E-4 0.0 1.8744321752693809 4.884513811776888E-4 80-81 9.769027623553776E-4 1.6281712705922962E-4 0.0 2.060369334371021 5.698599447073036E-4 82-83 9.769027623553776E-4 1.6281712705922962E-4 0.0 2.2777301989950924 6.512685082369185E-4 84-85 0.0010583113258849923 1.6281712705922962E-4 0.0 2.632508718857154 6.512685082369185E-4 86-87 0.0011397198894146073 1.6281712705922962E-4 0.0 3.0152103760098736 6.512685082369185E-4 88-89 0.0011397198894146073 1.6281712705922962E-4 0.0 3.476389888405141 6.512685082369185E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2110 0.0 41.913727 1 GTATCAA 3375 0.0 36.065502 1 GGACCGA 145 2.395791E-8 29.483213 6 ATCAACG 4230 0.0 28.878633 3 TCAACGC 4240 0.0 28.81288 4 TATCAAC 4290 0.0 28.472414 2 CAACGCA 4285 0.0 28.399359 5 AACGCAG 4415 0.0 27.542896 6 AGGACCG 175 4.9112714E-9 27.163227 5 GTACATG 3245 0.0 26.081411 1 CCCTATA 185 8.894858E-9 25.690748 2 TAAGGTA 170 1.1129305E-7 25.16593 4 CCGAGTG 170 1.12087946E-7 25.147448 9 TACATGG 3340 0.0 24.760035 2 ACATGGG 3305 0.0 24.73665 3 ACGCAGA 5070 0.0 23.984592 7 CGCAGAG 5040 0.0 23.938862 8 TAGCGCG 50 0.001653167 23.751335 28-29 ACCGAGT 200 2.0498192E-8 23.750366 8 GCAGCGT 100 0.0016526193 23.750366 6 >>END_MODULE