FastQCFastQC Report
Thu 26 May 2016
SRR937703_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937703_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614186
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45740.7447255391689163No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38120.6206588883497833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25660.4177887480339832No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC9800.159560784518045No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9220.1501173911486097No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA8620.14034836352505592No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.10306324142849235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27450.041.3730051
GTATCAA45100.037.298231
GCCGCGC1107.004928E-834.5447468
AGGTAAG2900.034.4014432
TAAGGTG3550.033.4554635
TATCAAC57300.029.3497242
TCAACGC56750.029.2993224
ATCAACG57100.029.202933
CAACGCA57350.028.9927925
GTAAGGT3800.028.7540914
AACGCAG58700.028.4832296
GGTAAGG4000.027.3163853
CGCGCTA350.00839263327.14229846-47
GTACTAA908.922618E-426.3990921
ACGCAGA65850.025.3183867
CGCAGAG65800.025.2654368
AGCCGCG1551.409102E-624.5156237
CGGGACT1000.001651403823.753384
GCGCGCT500.001653908323.7495146-47
GAGTACT40300.023.7495112-13
Produced by FastQC (version 0.11.5)