##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937703_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 614186 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3506901818016 33.0 31.0 34.0 31.0 34.0 2 32.64303484612153 34.0 31.0 34.0 31.0 34.0 3 32.72674564382777 34.0 31.0 34.0 31.0 34.0 4 36.312696805202336 37.0 37.0 37.0 35.0 37.0 5 36.24642209363287 37.0 37.0 37.0 35.0 37.0 6 36.30151289674463 37.0 37.0 37.0 35.0 37.0 7 36.25457271901346 37.0 37.0 37.0 35.0 37.0 8 36.22982288752919 37.0 37.0 37.0 35.0 37.0 9 37.967630326969356 39.0 38.0 39.0 35.0 39.0 10-11 37.892732006265206 39.0 38.0 39.0 35.0 39.0 12-13 37.80562809963105 39.0 38.0 39.0 35.0 39.0 14-15 39.07186585171267 40.0 38.0 41.0 36.0 41.0 16-17 39.02969377354743 40.0 38.0 41.0 36.0 41.0 18-19 39.04035341085599 40.0 38.0 41.0 36.0 41.0 20-21 38.955594070851504 40.0 38.0 41.0 35.5 41.0 22-23 38.89108266876809 40.0 38.0 41.0 35.0 41.0 24-25 38.89608034048318 40.0 38.0 41.0 35.0 41.0 26-27 38.75871315855457 40.0 38.0 41.0 35.0 41.0 28-29 38.596919337138914 40.0 38.0 41.0 35.0 41.0 30-31 38.611701504104616 40.0 38.0 41.0 35.0 41.0 32-33 38.50417788748034 40.0 38.0 41.0 35.0 41.0 34-35 38.35352808432624 40.0 38.0 41.0 34.5 41.0 36-37 38.190859120852636 40.0 38.0 41.0 34.0 41.0 38-39 38.01901541226925 40.0 37.5 41.0 34.0 41.0 40-41 37.80233756549319 40.0 37.0 41.0 33.0 41.0 42-43 37.54391340733915 40.0 37.0 41.0 33.0 41.0 44-45 37.18065862784238 40.0 37.0 41.0 32.5 41.0 46-47 36.71033693376273 40.0 36.0 41.0 31.0 41.0 48-49 36.28002347822972 40.0 36.0 41.0 30.0 41.0 50-51 35.95960751303351 39.0 35.5 41.0 29.5 41.0 52-53 35.70219933375232 39.0 35.0 41.0 28.5 41.0 54-55 35.458188561771195 39.0 35.0 40.0 28.0 41.0 56-57 35.16178730873058 38.5 35.0 40.0 27.0 41.0 58-59 34.88617780281544 38.0 35.0 40.0 26.5 41.0 60-61 34.54455816316229 38.0 34.0 40.0 26.0 41.0 62-63 34.16382089464755 37.0 34.0 40.0 25.5 41.0 64-65 33.743491385345806 37.0 34.0 39.5 24.5 41.0 66-67 33.32750420882273 36.0 33.0 39.0 23.0 40.5 68-69 32.89008704203613 35.5 33.0 39.0 22.0 40.0 70-71 32.4871691963021 35.0 32.5 38.0 21.0 40.0 72-73 32.013390894614986 35.0 32.0 37.5 19.5 39.5 74-75 31.55015174556242 35.0 31.0 37.0 18.0 39.0 76-77 30.360525150361617 34.0 30.0 35.5 17.5 38.0 78-79 30.90857899724188 34.0 31.0 36.0 17.0 38.0 80-81 30.72221362909607 34.0 31.0 35.5 13.5 37.0 82-83 30.364627327877876 34.0 31.0 35.0 7.5 37.0 84-85 29.996348825925697 34.0 31.0 35.0 6.0 36.5 86-87 29.667191209177673 34.0 30.0 35.0 2.0 36.0 88-89 29.25154920496397 34.0 30.0 35.0 2.0 36.0 90-91 28.752425975193184 34.0 29.0 35.0 2.0 35.0 92-93 28.350662502890003 34.0 29.0 35.0 2.0 35.0 94-95 27.920071444155354 33.5 28.5 35.0 2.0 35.0 96-97 27.392841907825968 33.0 27.0 35.0 2.0 35.0 98-99 26.56845320472951 33.0 25.5 35.0 2.0 35.0 100-101 23.89711829966818 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2645371017483882 1101 2 0.2577181178862986 1101 3 0.1642713491419272 1101 4 0.1763820744350113 1101 5 0.04830495358302045 1101 6 0.07208254579675355 1101 7 0.15654533028138218 1101 8 0.05004589341658061 1101 9 0.1111118480281732 1101 10-11 0.22009527015883634 1101 12-13 0.14137098251456592 1101 14-15 0.17538709285032184 1101 16-17 0.2285580351307459 1101 18-19 0.13934173003573846 1101 20-21 0.29062151620974674 1101 22-23 0.06414110960052 1101 24-25 0.0499292018639963 1101 26-27 0.029565566235476126 1101 28-29 0.11953386322070969 1101 30-31 -0.05182283461471826 1101 32-33 0.06712089256046028 1101 34-35 0.07692502141764379 1101 36-37 -0.10979388677807833 1101 38-39 -0.08609748260841599 1101 40-41 -0.09999770353967818 1101 42-43 -0.10139124967179924 1101 44-45 -0.1519927065426998 1101 46-47 -0.23400451531758648 1101 48-49 -0.07892182336567544 1101 50-51 -0.0685199353849626 1101 52-53 -0.3185965745612833 1101 54-55 -0.3320231029691385 1101 56-57 -0.48559846888561964 1101 58-59 -0.7059445797080173 1101 60-61 -0.4873751211797881 1101 62-63 -0.45828154156502876 1101 64-65 -0.40953286867056704 1101 66-67 -0.46775079705915346 1101 68-69 -0.39414262474540607 1101 70-71 -0.48801849062794034 1101 72-73 -0.7706648086289825 1101 74-75 -0.7184838751986327 1101 76-77 -0.830090016342929 1101 78-79 -0.7544914171729822 1101 80-81 -0.5688766310816895 1101 82-83 -0.4516398641598691 1101 84-85 -0.44638555963137705 1101 86-87 -0.5550415448353725 1101 88-89 -0.5301196211322043 1101 90-91 -0.46821474568771393 1101 92-93 -0.39801920958477055 1101 94-95 -0.5435059340094348 1101 96-97 -0.450451781088244 1101 98-99 -0.5721345643775884 1101 100-101 -0.40362868631240545 1102 1 0.13978875547286407 1102 2 0.03873144355019065 1102 3 0.03250066560248399 1102 4 -0.03850623924536478 1102 5 -0.038479630828831546 1102 6 -0.1299594444247063 1102 7 -0.002427922522755921 1102 8 -0.04047754633032952 1102 9 0.12048295338719583 1102 10-11 -0.005134813246392866 1102 12-13 0.04812728584118986 1102 14-15 0.1736614673110708 1102 16-17 0.09048402496873109 1102 18-19 8.602805102810862E-4 1102 20-21 -0.028199785678857836 1102 22-23 0.02336122272485852 1102 24-25 -0.1040828775147773 1102 26-27 0.022291575247074036 1102 28-29 -0.11890204896413081 1102 30-31 -0.049689769712024656 1102 32-33 -0.013396315760878963 1102 34-35 0.006162390797378237 1102 36-37 -0.20247435844906647 1102 38-39 -0.014627824857477378 1102 40-41 0.09957568347979162 1102 42-43 -0.02434925945033939 1102 44-45 -0.1383674548889715 1102 46-47 -0.2947249639802223 1102 48-49 -0.32713628027297403 1102 50-51 -0.18097319969327685 1102 52-53 0.015503703456168694 1102 54-55 -0.346415299306976 1102 56-57 -0.40461390364738037 1102 58-59 -0.3414900691145988 1102 60-61 -0.3846045335084298 1102 62-63 -0.3622337033903591 1102 64-65 -0.5043823557763076 1102 66-67 -0.8546504231736165 1102 68-69 -0.6283004236141636 1102 70-71 -0.7109956740235859 1102 72-73 -0.7624561038068194 1102 74-75 -0.7932609818998095 1102 76-77 -0.7190987403387119 1102 78-79 -0.48080001654025395 1102 80-81 -0.7678148920346182 1102 82-83 -0.7398313127266398 1102 84-85 -0.5863769315036791 1102 86-87 -0.4626846279530028 1102 88-89 -0.3962158726900604 1102 90-91 -0.6618083608732199 1102 92-93 -0.5890483534827702 1102 94-95 -0.9471105740247729 1102 96-97 -1.005623864301743 1102 98-99 -0.7713364625656816 1102 100-101 -0.24496700478618294 1103 1 0.23749624190023155 1103 2 0.19035528887969377 1103 3 0.21818009453323128 1103 4 0.1107750530999354 1103 5 0.02606235356596187 1103 6 0.1109448961621311 1103 7 0.11284206888640824 1103 8 0.15298456099313995 1103 9 0.2971397893022072 1103 10-11 0.16092739504154707 1103 12-13 0.17761976221638776 1103 14-15 0.25597297814703523 1103 16-17 0.1456535832934307 1103 18-19 0.05113549727393263 1103 20-21 0.04989933663871682 1103 22-23 0.06284143280182519 1103 24-25 0.13378145811240216 1103 26-27 0.012691093451678626 1103 28-29 0.1346051381124198 1103 30-31 0.03375205615081711 1103 32-33 0.07976223389326975 1103 34-35 0.24538066891022225 1103 36-37 0.4395855382837581 1103 38-39 0.35875807199933973 1103 40-41 0.40314663759408376 1103 42-43 0.5510619050951817 1103 44-45 0.6939440559469929 1103 46-47 0.5041597770990336 1103 48-49 0.578693311175158 1103 50-51 0.5724564927193256 1103 52-53 0.484901060666715 1103 54-55 0.37064946851900515 1103 56-57 0.5641446761189783 1103 58-59 0.6387742541134358 1103 60-61 0.6946116190365004 1103 62-63 0.6174859430568418 1103 64-65 0.7090279851116463 1103 66-67 0.7178956529394256 1103 68-69 0.8118283060933109 1103 70-71 0.7543232497305183 1103 72-73 0.757796573080288 1103 74-75 0.5027265658147613 1103 76-77 0.5233521723270158 1103 78-79 0.5435764835350234 1103 80-81 0.7931313399534083 1103 82-83 0.6645325225064163 1103 84-85 0.888255743789248 1103 86-87 0.8438964403220091 1103 88-89 0.9119328019297726 1103 90-91 1.1926478037165893 1103 92-93 1.1363315917838541 1103 94-95 0.9225389729635616 1103 96-97 0.957431108198719 1103 98-99 0.8292416476273026 1103 100-101 1.230440617146133 1104 1 0.29094426549108476 1104 2 0.1890046675939061 1104 3 0.11136564738754373 1104 4 0.10072417969816883 1104 5 -0.026896069808792333 1104 6 0.16976163936400468 1104 7 -0.012588441648439641 1104 8 0.03414677060955995 1104 9 0.17416009364220741 1104 10-11 0.27318372045123596 1104 12-13 0.2506546082455614 1104 14-15 0.12129352559886541 1104 16-17 0.30608910238220943 1104 18-19 0.1698607358836881 1104 20-21 0.10307582907524449 1104 22-23 0.23391450141338765 1104 24-25 -0.033130008662318744 1104 26-27 -0.1386544630042934 1104 28-29 -0.13900963093134067 1104 30-31 0.2697689051513592 1104 32-33 0.26110846565988055 1104 34-35 0.2304337933474656 1104 36-37 0.37787058690612696 1104 38-39 0.21301618698222313 1104 40-41 0.10929505961821206 1104 42-43 0.3321796567609496 1104 44-45 0.25804932562104455 1104 46-47 -0.04888024631173238 1104 48-49 0.07157159768695465 1104 50-51 0.023859450579948316 1104 52-53 0.15316877631591552 1104 54-55 0.3174195139899254 1104 56-57 0.3822286509974262 1104 58-59 0.3736880957305786 1104 60-61 0.3637305074751822 1104 62-63 0.23561465726537278 1104 64-65 0.5329013418557551 1104 66-67 0.40112712691745145 1104 68-69 0.3379023313949503 1104 70-71 0.2305030736995448 1104 72-73 0.4569484954645837 1104 74-75 0.524845803163938 1104 76-77 0.47624222049917364 1104 78-79 0.6753788313650269 1104 80-81 0.9580787782750342 1104 82-83 0.9876419340857439 1104 84-85 0.9126696304271036 1104 86-87 0.9766653265096572 1104 88-89 0.7749333759438208 1104 90-91 1.042156233844448 1104 92-93 1.055973845970783 1104 94-95 0.7338149724233141 1104 96-97 0.6126878387947983 1104 98-99 0.5462864882539904 1104 100-101 0.2694341791846746 1105 1 0.24165380655388446 1105 2 0.09875433821314061 1105 3 0.15130992714160385 1105 4 0.08823515905697832 1105 5 0.11523859202695519 1105 6 0.09848501441889823 1105 7 0.09493379155313875 1105 8 0.022741961650403653 1105 9 0.179004735715111 1105 10-11 0.22584209770200658 1105 12-13 0.20890559638785788 1105 14-15 0.011884215102597295 1105 16-17 0.17274822073969887 1105 18-19 0.16946273412699497 1105 20-21 0.07768263078801851 1105 22-23 0.1479049493712168 1105 24-25 0.2066797739463766 1105 26-27 0.10008483212087782 1105 28-29 0.11979101136114423 1105 30-31 0.18582827603410124 1105 32-33 0.13085895973102168 1105 34-35 0.17685452237689248 1105 36-37 0.2084659426373463 1105 38-39 0.09670017358740779 1105 40-41 0.3744088332238391 1105 42-43 0.23864238183779918 1105 44-45 -0.15785221697886698 1105 46-47 0.12224678486963825 1105 48-49 -0.007267060635399503 1105 50-51 0.3022067825957606 1105 52-53 0.446887210659348 1105 54-55 0.41032890683138135 1105 56-57 0.15727188222272304 1105 58-59 0.13850679906830266 1105 60-61 0.10145078086121373 1105 62-63 -0.26706156144344817 1105 64-65 -0.21933570250086376 1105 66-67 -0.062057710588035775 1105 68-69 0.0810642494889251 1105 70-71 -0.15180163095570265 1105 72-73 -0.049179359168704195 1105 74-75 0.04992677593513051 1105 76-77 -0.008438142917601255 1105 78-79 -0.08806745212201506 1105 80-81 0.24452338740986335 1105 82-83 0.18493758076997935 1105 84-85 0.19122625647014146 1105 86-87 0.03097884210034252 1105 88-89 -0.009579552251175016 1105 90-91 -0.07816724551435783 1105 92-93 0.10549418523296694 1105 94-95 -0.035103835218318835 1105 96-97 0.06077605794279606 1105 98-99 0.049909676659787294 1105 100-101 0.31108712165283237 1106 1 0.16372738514920115 1106 2 0.16592774451697778 1106 3 0.11566586106275878 1106 4 0.1499763164503065 1106 5 0.17900670796898055 1106 6 0.12235245132981021 1106 7 0.0171391224541253 1106 8 -0.026269896057101505 1106 9 0.22998274321485468 1106 10-11 -0.016105809463290655 1106 12-13 -0.2733838532929056 1106 14-15 -0.02849279918746106 1106 16-17 -0.045980876250276026 1106 18-19 0.17493552220847164 1106 20-21 0.20952614958806492 1106 22-23 0.2087542450031279 1106 24-25 0.2531680682607558 1106 26-27 0.31398976776494436 1106 28-29 0.27264902291481263 1106 30-31 0.3034628154077694 1106 32-33 0.43650910668552 1106 34-35 0.027442340356010675 1106 36-37 0.48099558690612554 1106 38-39 0.36791736219590376 1106 40-41 0.393816961327623 1106 42-43 0.40795422941052095 1106 44-45 0.5530680222022397 1106 46-47 0.45715607847458983 1106 48-49 0.43853206777242093 1106 50-51 0.24733701468251468 1106 52-53 0.4065608703330028 1106 54-55 -0.007113071480169708 1106 56-57 0.12794179202307276 1106 58-59 0.3242623478673252 1106 60-61 0.25103018696236035 1106 62-63 0.3117364521371684 1106 64-65 -0.4106349829305813 1106 66-67 -0.4336752235098942 1106 68-69 0.01187401942913624 1106 70-71 0.5832140779730466 1106 72-73 0.3928192219603126 1106 74-75 0.13219088863402462 1106 76-77 0.18143719913165413 1106 78-79 0.03612936555306234 1106 80-81 0.004526427847686421 1106 82-83 -0.30152740352109575 1106 84-85 -0.5283693652993939 1106 86-87 -0.15220753673820653 1106 88-89 -0.25892079072285057 1106 90-91 -0.08990840290769242 1106 92-93 -0.07066878223434614 1106 94-95 -0.05873310449976188 1106 96-97 0.6511226678546223 1106 98-99 -0.04217505020754686 1106 100-101 -0.5866021456016526 1107 1 0.31278271578347727 1107 2 0.2789169482956595 1107 3 0.12406667077935651 1107 4 0.11205458905489252 1107 5 0.1645330237584588 1107 6 0.1883106159721919 1107 7 0.2990891899305055 1107 8 0.22109852499552574 1107 9 0.3274861170340202 1107 10-11 0.3823759719132269 1107 12-13 0.22872478368415727 1107 14-15 0.30148358407839027 1107 16-17 0.48586797665121395 1107 18-19 0.4606136598603001 1107 20-21 0.2317764869699772 1107 22-23 0.304638185623908 1107 24-25 0.3207625351973249 1107 26-27 0.29966790541676147 1107 28-29 0.3666098866125225 1107 30-31 0.24715377357241408 1107 32-33 0.14022030776514072 1107 34-35 -0.04222702536598888 1107 36-37 -0.005627220111414033 1107 38-39 0.0454814647600017 1107 40-41 0.16060171166500226 1107 42-43 0.4183455924334609 1107 44-45 0.2021739601239645 1107 46-47 0.17389022152452327 1107 48-49 0.058505077219116686 1107 50-51 -0.10470414591128474 1107 52-53 -0.2630410190057262 1107 54-55 -0.08604357080539415 1107 56-57 -0.181504901634149 1107 58-59 0.08499109280659667 1107 60-61 0.04259563905412733 1107 62-63 -0.05988972869953102 1107 64-65 0.16649052314230062 1107 66-67 0.36887200995838754 1107 68-69 0.4571000653130781 1107 70-71 0.23383823451825947 1107 72-73 -0.12300398991553507 1107 74-75 -0.13844001554950935 1107 76-77 0.23423746903718978 1107 78-79 0.10186823194982608 1107 80-81 0.24106488938614845 1107 82-83 0.2208747557231696 1107 84-85 0.3946232122984483 1107 86-87 0.5107479288488399 1107 88-89 0.4483160513824167 1107 90-91 0.6414343771192996 1107 92-93 0.5182819600058686 1107 94-95 0.554812779440855 1107 96-97 0.6237441253445013 1107 98-99 0.5857601724645178 1107 100-101 0.2659619569624496 1108 1 0.09158699598753373 1108 2 0.21382736262936675 1108 3 0.08118686488163718 1108 4 0.029063423196987515 1108 5 0.10028330371366678 1108 6 0.09880271501411642 1108 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46-47 -0.28792104492284665 2316 48-49 -0.4087886453686025 2316 50-51 -0.3267264869200517 2316 52-53 -0.26360639729519875 2316 54-55 -0.5261134721211889 2316 56-57 -0.2886697865025738 2316 58-59 -0.43186745982497854 2316 60-61 -0.4598372008581535 2316 62-63 -0.6045546135679558 2316 64-65 -0.33580525536647343 2316 66-67 -0.24166801197143428 2316 68-69 -0.43217145332727824 2316 70-71 -0.13418876657823375 2316 72-73 -0.2838284142576377 2316 74-75 -0.4402149607249477 2316 76-77 -0.4183975364452692 2316 78-79 -0.3202808908571946 2316 80-81 -0.41030229811385155 2316 82-83 -0.6336688298031454 2316 84-85 -0.40547273068400713 2316 86-87 -0.5115074165458999 2316 88-89 -0.5955093323895149 2316 90-91 -0.27663716893333046 2316 92-93 -0.18686469569588482 2316 94-95 -0.23526054841002164 2316 96-97 -0.23876615426075887 2316 98-99 -0.42376559507934175 2316 100-101 0.06544112362911747 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 5.0 5 11.0 6 17.0 7 41.0 8 112.0 9 231.0 10 421.0 11 790.0 12 1327.0 13 1925.0 14 2498.0 15 3856.0 16 5452.0 17 8383.0 18 7692.0 19 4211.0 20 3163.0 21 3019.0 22 3048.0 23 3134.0 24 3388.0 25 4075.0 26 4829.0 27 5902.0 28 7315.0 29 9198.0 30 11435.0 31 14799.0 32 19472.0 33 26885.0 34 38764.0 35 60014.0 36 95775.0 37 143293.0 38 110169.0 39 9534.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.62971648183432 19.665673294575537 15.533408243359878 21.17120198023027 2 15.77274636673581 20.968403708322885 39.84867776211115 23.410172162830154 3 19.417244938829604 25.347207523453807 30.544330219184417 24.69121731853217 4 13.074215302856137 17.36867984617038 36.618711595510156 32.938393255463325 5 13.364018521866456 38.15680391936505 33.7377458764742 14.741431682294287 6 31.557541200874002 36.71933258003276 16.861830129634996 14.861296089458243 7 28.09149020003712 34.30801092828557 20.199092782968027 17.401406088709283 8 27.11328490066527 34.555981412796775 19.128081721172414 19.20265196536554 9 27.32983167965405 16.544825183250676 20.229051134346925 35.89629200274835 10-11 25.136684978166222 27.187041059223098 26.27453247061965 21.401741491991025 12-13 26.30501751963632 24.67362350366675 27.51624962550963 21.505109351187297 14-15 23.19883553190727 25.81595803225733 26.246120881947814 24.739085553887584 16-17 21.43056012348051 29.099572442224343 27.34326409263643 22.126603341658715 18-19 21.365677498347406 26.670585132191228 31.137065970243544 20.826671399217826 20-21 22.297805550761495 25.613820569013296 31.470759672151434 20.617614208073775 22-23 22.081936491241212 25.498100728935842 31.47368129694244 20.94628148288051 24-25 21.73202308430624 25.787570001473505 31.60415413308889 20.87625278113137 26-27 21.22686131196707 26.127891471025457 31.365469563334347 21.279777653673126 28-29 21.128097501646494 26.285098370277986 31.544837096996098 21.041967031079423 30-31 21.264893696697744 26.250109901560766 31.272529819956823 21.21246658178467 32-33 21.168424548915148 26.242538905152514 31.16775699869421 21.421279547238132 34-35 21.758392408814267 26.216813799077155 30.991181824398474 21.033611967710108 36-37 21.935130400237064 25.949630893572955 30.842204153139274 21.273034553050703 38-39 21.383505973760393 26.383864171439924 31.074299967762208 21.15832988703748 40-41 21.58893029003689 26.34323297467113 31.00928798618647 21.058548749105515 42-43 21.618050201144907 26.53785684800512 30.456701939963477 21.387391010886496 44-45 21.705802476774135 26.506302650988463 30.36360320814867 21.42429166408873 46-47 22.226410240546024 27.009488982165013 29.510441462358312 21.253659314930655 48-49 22.012142901336077 27.173934280494834 29.081174106866648 21.73274871130244 50-51 22.150700276463482 27.517722644280397 28.841914338653112 21.48966274060301 52-53 22.47845115323371 27.266333000100946 28.78118355026002 21.474032296405323 54-55 22.175077541783015 27.67049016175908 28.60750425360437 21.546928042853537 56-57 22.22242122093307 27.819585597848207 28.336855610515382 21.62113757070334 58-59 21.994640060177208 27.386817674124774 28.532887431494693 22.085654834203318 60-61 21.860560156043935 27.89806345309076 28.726639812695176 21.514736578170133 62-63 22.059197051056195 27.631287590404206 29.01971064140179 21.289804717137805 64-65 21.98185891570306 27.589280771622924 28.83019150550485 21.59866880716916 66-67 21.78533864334257 27.888701468284854 28.61388895220666 21.712070936165915 68-69 22.280384118166157 27.524398146489826 28.61421458646078 21.581003148883237 70-71 22.177402285301195 27.833018010830596 28.262692409139902 21.726887294728307 72-73 22.145327311270528 27.85141634618829 28.08782681467829 21.915429527862894 74-75 22.224700660711903 28.1766435574891 27.682574985427866 21.916080796371133 76-77 22.05984831956443 27.828703356963523 28.34605477819423 21.765393545277814 78-79 21.95499408973829 27.78523118403871 28.217999107762143 22.041775618460857 80-81 22.126440524531656 28.299000628474108 27.830168711107056 21.744390135887173 82-83 22.23223553259821 28.26545855669935 27.619722150989322 21.882583759713114 84-85 21.840487800906892 28.378990043716467 27.746363066502767 22.03415908887387 86-87 22.073524958237407 28.38065341769431 27.632101676039504 21.913719948028774 88-89 21.895484429798138 28.18909906770913 27.42605660174605 22.48935990074668 90-91 21.768112071674807 28.07222519854032 27.83130627073132 22.328356459053552 92-93 22.012980034973804 27.751581799294662 27.857333589503952 22.378104576227585 94-95 21.721188695281235 27.46016678986496 28.125356162465444 22.693288352388365 96-97 21.92283770714409 27.463911583787322 27.945117602810875 22.668133106257713 98-99 21.550232098459894 27.820391123760334 27.684681501646914 22.944695276132855 100-101 21.511735368956746 27.871348600508906 27.970035623409668 22.64688040712468 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 263.0 1 195.5 2 156.5 3 198.5 4 195.0 5 192.5 6 311.5 7 647.0 8 1089.5 9 1317.0 10 1208.5 11 1082.0 12 1140.0 13 1199.0 14 1254.5 15 1324.5 16 1357.5 17 1438.0 18 1554.0 19 1579.5 20 1672.0 21 1872.5 22 2046.0 23 2424.0 24 2843.5 25 3277.0 26 3869.0 27 4570.0 28 5359.0 29 6330.5 30 7411.5 31 8735.0 32 10712.5 33 11911.0 34 12640.0 35 13978.5 36 15998.5 37 17703.5 38 18785.5 39 19514.5 40 19628.5 41 19921.0 42 19390.0 43 19597.5 44 20423.0 45 20587.5 46 20816.0 47 21216.0 48 22355.0 49 22043.0 50 20834.0 51 19938.0 52 18952.0 53 19028.0 54 19612.5 55 18594.5 56 16420.0 57 15008.5 58 14455.0 59 13052.0 60 10290.5 61 8167.0 62 6732.5 63 4933.0 64 3332.5 65 2390.5 66 1759.0 67 1395.5 68 1098.0 69 837.5 70 579.0 71 384.5 72 312.0 73 242.0 74 160.5 75 121.5 76 109.5 77 75.0 78 50.5 79 38.0 80 22.0 81 16.0 82 14.5 83 10.5 84 6.0 85 4.0 86 3.0 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018886786738870637 2 0.0 3 0.0 4 0.0 5 0.03402877955537899 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002930708287066133 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.884513811776888E-4 24-25 4.07042817648074E-4 26-27 0.0014653541435330666 28-29 2.442256905888444E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.954941988257629E-4 42-43 0.012455510220031066 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.6281712705922962E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.698599447073036E-4 84-85 1.6281712705922962E-4 86-87 0.0 88-89 0.0 90-91 0.01286255303767914 92-93 0.001953805524710755 94-95 0.0 96-97 0.0 98-99 0.0011397198894146073 100-101 0.02010791519181486 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 614186.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.171234567574125 #Duplication Level Percentage of deduplicated Percentage of total 1 79.040220775438 45.978672230008506 2 12.092168174425542 14.068327026101258 3 3.750189514718688 6.544594618006734 4 1.6677832899031297 3.880680518593418 5 0.8851387686399318 2.57448074677036 6 0.5596432294307457 1.9533082544022369 7 0.3685450333510604 1.5007103708645277 8 0.2589609333882689 1.2051261759973544 9 0.18196000734383433 0.9526354442204128 >10 1.0730777689499809 11.457476634879777 >50 0.08362762994503163 3.3447446157755705 >100 0.036409293614934855 4.06468910035447 >500 0.0014222380318326463 0.6623631373382586 >1k 8.533428190995877E-4 1.8121911266871367 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4574 0.7447255391689163 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3812 0.6206588883497833 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2566 0.4177887480339832 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 980 0.159560784518045 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 922 0.1501173911486097 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 862 0.14034836352505592 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 633 0.10306324142849235 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 1.6281712705922962E-4 0.0 0.0 0.0 0.0 14-15 1.6281712705922962E-4 0.0 0.0 0.0 8.140856352961481E-5 16-17 1.6281712705922962E-4 0.0 0.0 0.0 1.6281712705922962E-4 18-19 1.6281712705922962E-4 0.0 0.0 0.0 1.6281712705922962E-4 20-21 1.6281712705922962E-4 0.0 0.0 0.0 1.6281712705922962E-4 22-23 1.6281712705922962E-4 0.0 0.0 1.6281712705922962E-4 1.6281712705922962E-4 24-25 1.6281712705922962E-4 0.0 0.0 3.2563425411845923E-4 1.6281712705922962E-4 26-27 1.6281712705922962E-4 0.0 0.0 4.884513811776888E-4 1.6281712705922962E-4 28-29 2.442256905888444E-4 0.0 0.0 0.0017909883976515257 1.6281712705922962E-4 30-31 3.2563425411845923E-4 0.0 0.0 0.004151836740010356 1.6281712705922962E-4 32-33 4.07042817648074E-4 0.0 0.0 0.006756910772958029 1.6281712705922962E-4 34-35 4.884513811776888E-4 0.0 0.0 0.008629307734139169 1.6281712705922962E-4 36-37 4.884513811776888E-4 0.0 0.0 0.010420296131790695 1.6281712705922962E-4 38-39 6.512685082369185E-4 0.0 0.0 0.013513821545916058 1.6281712705922962E-4 40-41 7.326770717665332E-4 0.0 0.0 0.02018932375534447 1.6281712705922962E-4 42-43 8.14085635296148E-4 0.0 0.0 0.027109051655361732 1.6281712705922962E-4 44-45 8.14085635296148E-4 0.0 0.0 0.04404203286952161 1.6281712705922962E-4 46-47 8.14085635296148E-4 0.0 0.0 0.08148997209314442 1.6281712705922962E-4 48-49 8.14085635296148E-4 0.0 0.0 0.10713366960497309 1.6281712705922962E-4 50-51 8.14085635296148E-4 0.0 0.0 0.13872019225446364 1.6281712705922962E-4 52-53 8.14085635296148E-4 0.0 0.0 0.17559827153337915 1.6281712705922962E-4 54-55 8.14085635296148E-4 0.0 0.0 0.22175692705467076 1.6281712705922962E-4 56-57 8.14085635296148E-4 0.0 0.0 0.2805339099230526 1.6281712705922962E-4 58-59 8.14085635296148E-4 0.0 0.0 0.3529875314644098 1.6281712705922962E-4 60-61 8.14085635296148E-4 0.0 0.0 0.45051499057288835 1.6281712705922962E-4 62-63 8.14085635296148E-4 0.0 0.0 0.5662779679120007 1.6281712705922962E-4 64-65 8.14085635296148E-4 0.0 0.0 0.656234430612225 1.6281712705922962E-4 66-67 8.14085635296148E-4 0.0 0.0 0.7892560234196155 2.442256905888444E-4 68-69 8.14085635296148E-4 0.0 0.0 0.9526430104235524 3.2563425411845923E-4 70-71 8.14085635296148E-4 0.0 0.0 1.0902234827886015 3.2563425411845923E-4 72-73 8.14085635296148E-4 0.0 0.0 1.297896728352649 3.2563425411845923E-4 74-75 8.14085635296148E-4 0.0 0.0 1.4806589534766341 3.2563425411845923E-4 76-77 8.954941988257629E-4 0.0 0.0 1.66960822942887 3.2563425411845923E-4 78-79 9.769027623553776E-4 0.0 0.0 1.8951913589694327 3.2563425411845923E-4 80-81 0.0011397198894146073 0.0 0.0 2.0856873976287313 3.2563425411845923E-4 82-83 0.0011397198894146073 0.0 0.0 2.306223196230458 3.2563425411845923E-4 84-85 0.0011397198894146073 0.0 0.0 2.664176650070174 3.2563425411845923E-4 86-87 0.0011397198894146073 0.0 0.0 3.0544493036311477 3.2563425411845923E-4 88-89 0.0011397198894146073 0.0 0.0 3.526293337848795 3.2563425411845923E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2745 0.0 41.373005 1 GTATCAA 4510 0.0 37.29823 1 GCCGCGC 110 7.004928E-8 34.544746 8 AGGTAAG 290 0.0 34.401443 2 TAAGGTG 355 0.0 33.455463 5 TATCAAC 5730 0.0 29.349724 2 TCAACGC 5675 0.0 29.299322 4 ATCAACG 5710 0.0 29.20293 3 CAACGCA 5735 0.0 28.992792 5 GTAAGGT 380 0.0 28.754091 4 AACGCAG 5870 0.0 28.483229 6 GGTAAGG 400 0.0 27.316385 3 CGCGCTA 35 0.008392633 27.142298 46-47 GTACTAA 90 8.922618E-4 26.399092 1 ACGCAGA 6585 0.0 25.318386 7 CGCAGAG 6580 0.0 25.265436 8 AGCCGCG 155 1.409102E-6 24.515623 7 CGGGACT 100 0.0016514038 23.75338 4 GCGCGCT 50 0.0016539083 23.74951 46-47 GAGTACT 4030 0.0 23.74951 12-13 >>END_MODULE