FastQCFastQC Report
Thu 26 May 2016
SRR937702_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937702_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601659
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34120.5670986389300251No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27100.45042125190514887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17730.29468519543462324No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7770.12914291982667922No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6590.1095304815518425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18800.044.24371
GTATCAA33500.042.1388741
TCAACGC42150.033.370054
ATCAACG42050.033.3364033
TATCAAC42600.033.0175482
CAACGCA43350.032.3313065
GTACGTG600.0039371231.6869161
AACGCAG44700.031.3496326
AGCCCGT650.005840275429.2324096
CGCAGAG49100.028.4435528
ACGCAGA49450.028.2422317
AGGTAAG2253.6379788E-1227.4551322
TACATGG32900.027.2978612
ACTAGTA700.00838613727.144388
GTACATG33650.027.1198581
TGTAGCG908.92224E-426.3991662
ACATGGG31950.025.878623
GCAGAGT53450.025.7731959
TAAGGTG2701.8189894E-1224.6351175
GAGTACT28900.024.16225412-13
Produced by FastQC (version 0.11.5)