FastQCFastQC Report
Thu 26 May 2016
SRR937702_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937702_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601659
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43760.7273222872091999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35740.5940241897819196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24350.4047142982985379No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9860.16388020456770364No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC9740.16188571931941514No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA8060.13396292584337638No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.10736978919952997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24200.045.9627231
GGTAAGG2650.037.6591343
GTATCAA40900.037.074271
TAAGGTG3000.034.8496445
TAGGCCG550.002572738834.561634
GTAAGGT3200.032.6715434
TATCAAC51350.029.2444572
ATCAACG51550.029.2231833
TCAACGC51650.029.1666034
CAACGCA52350.028.77665
AGGTAAG3350.028.3714872
AACGCAG53450.028.259196
AAGGTGT4050.026.9744836
GGTGTGC4250.025.7050978
ACGCAGA59450.025.2473347
CGCAGAG60750.024.5506888
GGCGACA1751.4841316E-724.4278246
CGATTCG1000.001653027723.7492719
GTGCTAT2252.7830538E-923.2388921
GAGTACT38850.023.10740312-13
Produced by FastQC (version 0.11.5)