##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937702_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 601659 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46698212775011 33.0 31.0 34.0 31.0 34.0 2 32.67813661891537 34.0 31.0 34.0 31.0 34.0 3 32.74437014986895 34.0 31.0 34.0 31.0 34.0 4 36.30675016911573 37.0 37.0 37.0 35.0 37.0 5 36.24751395724156 37.0 37.0 37.0 35.0 37.0 6 36.299603263642695 37.0 37.0 37.0 35.0 37.0 7 36.25684648613251 37.0 37.0 37.0 35.0 37.0 8 36.237436820524586 37.0 37.0 37.0 35.0 37.0 9 37.96290423645287 39.0 38.0 39.0 35.0 39.0 10-11 37.90027324447901 39.0 38.0 39.0 35.0 39.0 12-13 37.82166891877293 39.0 38.0 39.0 35.0 39.0 14-15 39.08722548819182 40.0 38.0 41.0 36.0 41.0 16-17 39.05385858102347 40.0 38.0 41.0 36.0 41.0 18-19 39.07042278101051 40.0 38.5 41.0 36.0 41.0 20-21 38.97552683496798 40.0 38.0 41.0 35.5 41.0 22-23 38.8239933251227 40.0 38.0 41.0 35.0 41.0 24-25 38.87103492177462 40.0 38.0 41.0 35.0 41.0 26-27 38.74286846868409 40.0 38.0 41.0 35.0 41.0 28-29 38.597535314854426 40.0 38.0 41.0 35.0 41.0 30-31 38.604806875655484 40.0 38.0 41.0 35.0 41.0 32-33 38.496766440791205 40.0 38.0 41.0 35.0 41.0 34-35 38.358012595174344 40.0 38.0 41.0 34.5 41.0 36-37 38.17509170476964 40.0 38.0 41.0 34.0 41.0 38-39 38.012089073711195 40.0 37.5 41.0 34.0 41.0 40-41 37.80108416893955 40.0 37.0 41.0 33.0 41.0 42-43 37.53816779936808 40.0 37.0 41.0 33.0 41.0 44-45 37.16059678322771 40.0 37.0 41.0 32.5 41.0 46-47 36.702593994272505 40.0 36.0 41.0 31.0 41.0 48-49 36.27610490327578 40.0 36.0 41.0 30.0 41.0 50-51 35.97562988337248 39.0 35.5 41.0 29.5 41.0 52-53 35.71462406446176 39.0 35.0 41.0 28.5 41.0 54-55 35.47325478385597 39.0 35.0 40.0 28.0 41.0 56-57 35.18934063979762 38.5 35.0 40.0 27.0 41.0 58-59 34.904274680508394 38.0 35.0 40.0 26.5 41.0 60-61 34.5414769828092 38.0 34.0 40.0 26.0 41.0 62-63 34.17030244706719 37.0 34.0 40.0 26.0 41.0 64-65 33.777196883949216 37.0 34.0 39.5 25.0 41.0 66-67 33.34677367080023 36.0 33.0 39.0 23.0 40.5 68-69 32.90388575588498 36.0 33.0 39.0 22.0 40.0 70-71 32.47754957542395 35.0 32.5 38.5 20.5 40.0 72-73 31.9999958448224 35.0 32.0 37.5 19.5 39.5 74-75 31.512822878075454 35.0 31.0 37.0 18.0 39.0 76-77 30.31201561017121 34.0 30.0 35.5 17.0 38.0 78-79 30.876981811956604 34.0 31.0 36.0 16.5 38.0 80-81 30.685640869662052 34.0 31.0 35.5 12.0 37.0 82-83 30.34829862097966 34.0 31.0 35.0 7.5 37.0 84-85 29.983631924395713 34.0 31.0 35.0 4.5 36.5 86-87 29.596668544806942 34.0 30.0 35.0 2.0 36.0 88-89 29.243164317329253 34.0 30.0 35.0 2.0 36.0 90-91 28.75906202682915 34.0 29.0 35.0 2.0 35.0 92-93 28.317992417631913 34.0 29.0 35.0 2.0 35.0 94-95 27.890241814715644 33.5 28.5 35.0 2.0 35.0 96-97 27.38562790550794 33.0 27.0 35.0 2.0 35.0 98-99 26.557613199503372 33.0 25.5 35.0 2.0 35.0 100-101 23.974333467961088 30.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3776886620802884 1101 2 0.19839361042337345 1101 3 0.17137187321117864 1101 4 0.14549066316047288 1101 5 0.1585157988482706 1101 6 0.11722453817365874 1101 7 0.0675033488101775 1101 8 0.1141462294968889 1101 9 0.370284182748847 1101 10-11 0.30544186536310036 1101 12-13 0.2545096273997416 1101 14-15 0.23909858563371955 1101 16-17 0.25888109796174064 1101 18-19 0.2735056429685656 1101 20-21 0.32582213722595554 1101 22-23 0.33999036008607675 1101 24-25 0.25692942195058066 1101 26-27 0.2694485945056897 1101 28-29 0.3472840887713531 1101 30-31 0.3422672516736256 1101 32-33 0.37291955128991106 1101 34-35 0.11263210642121635 1101 36-37 0.10755708531058872 1101 38-39 -0.03619602501148478 1101 40-41 0.1583874647120993 1101 42-43 0.2617813755302336 1101 44-45 0.34663191698660967 1101 46-47 0.44321633521143866 1101 48-49 0.14190719985266043 1101 50-51 -0.030643426344560964 1101 52-53 -0.025264508303330047 1101 54-55 0.18839280405109804 1101 56-57 0.07683791497103698 1101 58-59 0.2086358264046524 1101 60-61 0.18389190782714593 1101 62-63 0.3181141344072884 1101 64-65 0.2843268655396045 1101 66-67 0.20741436689969106 1101 68-69 0.1458776895299181 1101 70-71 0.23024721388875236 1101 72-73 0.19631727291079315 1101 74-75 0.05601014627509926 1101 76-77 -0.16208164942053216 1101 78-79 0.062223263421412156 1101 80-81 0.2240987680298936 1101 82-83 0.27023800751202387 1101 84-85 0.007178577661900221 1101 86-87 -0.005541303086928906 1101 88-89 0.06315929428178535 1101 90-91 -0.04497208271598652 1101 92-93 0.12520446623674175 1101 94-95 0.15711508528493212 1101 96-97 0.04538061922642456 1101 98-99 0.21957221916293435 1101 100-101 0.2914403755083441 1102 1 -0.017766017662154354 1102 2 0.025244772642729174 1102 3 4.356759363162155E-4 1102 4 -0.01775493682584539 1102 5 -0.10931010692714693 1102 6 -0.028814399945488844 1102 7 0.09333200056108382 1102 8 0.008349982122744848 1102 9 -0.021261446936300388 1102 10-11 0.03247930610150007 1102 12-13 -0.0015098276656146936 1102 14-15 -0.05855520687564564 1102 16-17 -0.077500123048857 1102 18-19 0.022355396437191644 1102 20-21 0.08421438878592369 1102 22-23 -0.020276649490632792 1102 24-25 -0.2484676793815126 1102 26-27 -0.10161728247274482 1102 28-29 -0.20902551296890692 1102 30-31 -0.11297692401283399 1102 32-33 -0.06001551711846531 1102 34-35 -0.061289522878311686 1102 36-37 -0.08311717140235686 1102 38-39 -0.1947753325467474 1102 40-41 -0.24068471899148847 1102 42-43 -0.15749067662775218 1102 44-45 -0.21826779893087433 1102 46-47 -0.18780603531488538 1102 48-49 -0.3514455491549242 1102 50-51 -0.3100697569220685 1102 52-53 -0.3226461911229421 1102 54-55 -0.5614761945696998 1102 56-57 -0.4506296145684132 1102 58-59 -0.38412014718048937 1102 60-61 -0.45170035057444125 1102 62-63 -0.16107491652076789 1102 64-65 -0.27042860114406153 1102 66-67 -0.3830139026537154 1102 68-69 -0.5049962490479274 1102 70-71 -0.5471406263844116 1102 72-73 -0.5449483947764122 1102 74-75 -0.4245822131957979 1102 76-77 -0.7093579869174 1102 78-79 -0.5301641958981875 1102 80-81 -0.4279063694010503 1102 82-83 -0.21785214009581821 1102 84-85 -0.2749253122627344 1102 86-87 -0.3591493998273627 1102 88-89 -0.32172897747960505 1102 90-91 -0.5567178918380336 1102 92-93 -0.6490113525102146 1102 94-95 -0.4399005711291615 1102 96-97 -0.42901774624954214 1102 98-99 -0.6481241624001548 1102 100-101 -0.551959720585252 1103 1 0.2479959888158092 1103 2 0.13721944902021477 1103 3 0.28547532681738375 1103 4 0.10234844590717529 1103 5 -0.012138793797319636 1103 6 0.07486061356559048 1103 7 0.20317431615783477 1103 8 0.18030649666875576 1103 9 0.19276875170413632 1103 10-11 0.1525496004775846 1103 12-13 0.16308360833966162 1103 14-15 0.09252163531863289 1103 16-17 0.24646706065402668 1103 18-19 0.2699610861639883 1103 20-21 0.26760476889761975 1103 22-23 0.290470816492693 1103 24-25 0.14606226374328202 1103 26-27 0.1334828465718445 1103 28-29 0.17748418008143574 1103 30-31 0.24977624787383235 1103 32-33 0.25701506019983356 1103 34-35 0.31695819126769464 1103 36-37 0.32746430132364424 1103 38-39 0.26238075993888543 1103 40-41 0.3148880956144495 1103 42-43 0.5167342286108649 1103 44-45 0.4206775648388401 1103 46-47 0.37566754363209753 1103 48-49 0.17923379157794983 1103 50-51 0.3047440843052911 1103 52-53 0.07993800955426167 1103 54-55 0.09276885780182198 1103 56-57 -0.04218693647033689 1103 58-59 -0.1949870988972222 1103 60-61 -0.2640415724171703 1103 62-63 -0.3092032184630469 1103 64-65 -0.3598370180867718 1103 66-67 -0.20060330555593708 1103 68-69 -0.2541773122135993 1103 70-71 -0.1850078611904209 1103 72-73 -0.16713910135988996 1103 74-75 -0.2132413626779659 1103 76-77 -0.16789368006333305 1103 78-79 -0.10787771690377923 1103 80-81 -0.16596514902891712 1103 82-83 -0.29009045714845527 1103 84-85 -0.19083309133545256 1103 86-87 -0.22257261094211245 1103 88-89 -0.1391042251827237 1103 90-91 -0.17084698790088737 1103 92-93 -0.1022933498705001 1103 94-95 0.23796405337313686 1103 96-97 0.3095336822483574 1103 98-99 0.07259264170573942 1103 100-101 -0.12101787888730087 1104 1 0.172442052955752 1104 2 0.15045074153722027 1104 3 0.02817211982030443 1104 4 0.10792181811320489 1104 5 0.09440153662056616 1104 6 0.04605108975606953 1104 7 0.019607746672896553 1104 8 0.06172534992434464 1104 9 0.06282220165554975 1104 10-11 0.1548479483881735 1104 12-13 0.14025562000146863 1104 14-15 0.22267955562961106 1104 16-17 0.16584266803983638 1104 18-19 0.2619581707120915 1104 20-21 0.06998572127857017 1104 22-23 -0.042413051340147945 1104 24-25 -0.010298280474408728 1104 26-27 0.11446195249992996 1104 28-29 -0.017520679005052386 1104 30-31 0.2455245471935612 1104 32-33 0.30167006505892147 1104 34-35 0.3951238861170623 1104 36-37 0.3042062838309363 1104 38-39 0.2953812458475369 1104 40-41 0.3441786689866575 1104 42-43 0.16776678449036098 1104 44-45 0.1444360682402106 1104 46-47 0.19075743673219137 1104 48-49 0.38489219779757633 1104 50-51 0.5407499152090764 1104 52-53 0.49355074036086677 1104 54-55 0.5831770210671223 1104 56-57 0.6855462996812633 1104 58-59 0.8242184404613724 1104 60-61 0.7866508474079055 1104 62-63 0.8293317669596902 1104 64-65 1.017998258881157 1104 66-67 0.7362820200028608 1104 68-69 0.9068795390983624 1104 70-71 0.8368337325899446 1104 72-73 0.5684658549083288 1104 74-75 0.7001624274094951 1104 76-77 0.7076481080804982 1104 78-79 0.7182374434460712 1104 80-81 0.7174362115153023 1104 82-83 0.9099872882354134 1104 84-85 0.8581999504526898 1104 86-87 0.9728598477556538 1104 88-89 1.0418481557696602 1104 90-91 1.1958293969387626 1104 92-93 1.1545458143666245 1104 94-95 1.2350826150835346 1104 96-97 1.2372086093620602 1104 98-99 1.4083628069146812 1104 100-101 1.2315112756316466 1105 1 0.1302148615425125 1105 2 0.15850381249727263 1105 3 0.13249233448935627 1105 4 0.048258729264070155 1105 5 -0.06760800422618729 1105 6 0.12737786375726046 1105 7 0.04916648251069944 1105 8 0.011194640323864746 1105 9 0.1288895838857229 1105 10-11 0.09626416782764835 1105 12-13 0.057142918749235605 1105 14-15 0.09052541132430747 1105 16-17 0.17479466565462332 1105 18-19 0.14511559468347457 1105 20-21 0.28229937661548377 1105 22-23 0.28688778348394095 1105 24-25 0.28838090855362 1105 26-27 0.009031123638870042 1105 28-29 0.04558605921796044 1105 30-31 -0.004102763480268834 1105 32-33 0.1789211085890301 1105 34-35 0.09406693918802489 1105 36-37 0.12658106021734739 1105 38-39 0.10593729832218912 1105 40-41 0.24067837083519805 1105 42-43 0.28278616433532733 1105 44-45 0.2822014230404477 1105 46-47 0.39471837889080774 1105 48-49 0.336536503104675 1105 50-51 0.2999627953521937 1105 52-53 0.4603679735153108 1105 54-55 0.2867011713354586 1105 56-57 0.2799783211481781 1105 58-59 0.5033105692628013 1105 60-61 0.6412349261198926 1105 62-63 0.40763677590422986 1105 64-65 0.5895233036038832 1105 66-67 0.4239892352682162 1105 68-69 0.37127578238995085 1105 70-71 0.24892377433115342 1105 72-73 0.32416800159885284 1105 74-75 0.41014901059375575 1105 76-77 0.3193818588258779 1105 78-79 0.4429288566839986 1105 80-81 0.2928625681044501 1105 82-83 0.4334279560946861 1105 84-85 0.3239632181927057 1105 86-87 0.037827051094946995 1105 88-89 -0.15692942324048076 1105 90-91 0.1680834219834857 1105 92-93 0.26591283881504424 1105 94-95 0.01656442784441836 1105 96-97 0.5314796290400565 1105 98-99 0.9777739577793199 1105 100-101 0.8917483060430982 1106 1 0.16662040966917857 1106 2 0.20576470279306847 1106 3 0.1889236228263158 1106 4 0.09699663441246997 1106 5 0.15980567823713443 1106 6 0.19867968027043048 1106 7 0.1016184347156468 1106 8 0.1257600860632877 1106 9 0.16268526110778936 1106 10-11 0.11246651886245473 1106 12-13 0.11608728332812746 1106 14-15 0.3683225082014232 1106 16-17 0.2835864890151214 1106 18-19 0.1643329202110948 1106 20-21 0.2537198562151133 1106 22-23 0.24982976763112674 1106 24-25 0.23651868679345966 1106 26-27 0.20346829852531556 1106 28-29 0.20367504577784246 1106 30-31 0.425801768302442 1106 32-33 0.37658656472491714 1106 34-35 0.3385290297376429 1106 36-37 0.4407944601836391 1106 38-39 0.584260671365243 1106 40-41 0.525027032380109 1106 42-43 0.6658546268417282 1106 44-45 0.7700088805314493 1106 46-47 0.849803862917895 1106 48-49 0.7759125718790756 1106 50-51 0.8491667488764065 1106 52-53 0.7300253562593397 1106 54-55 0.7534575821893625 1106 56-57 0.5513529129077241 1106 58-59 0.5002955947699874 1106 60-61 0.6482991440010935 1106 62-63 0.612142388202173 1106 64-65 0.46676078393126375 1106 66-67 0.5591844916127471 1106 68-69 0.6502613026254096 1106 70-71 0.772421574941319 1106 72-73 0.6219478188361798 1106 74-75 0.5505281588724245 1106 76-77 0.7046604661299334 1106 78-79 0.8720500686965735 1106 80-81 0.8376032121833035 1106 82-83 0.7793326456168437 1106 84-85 0.7460990820492164 1106 86-87 0.9654784182299352 1106 88-89 0.9093498257763386 1106 90-91 1.0433043468385605 1106 92-93 1.1725902365443126 1106 94-95 1.255620357234502 1106 96-97 1.3841975873179706 1106 98-99 1.2226931342359926 1106 100-101 1.3225383297398636 1107 1 0.3107753862890874 1107 2 0.2282712543577361 1107 3 0.2541977608459405 1107 4 0.19035771554909786 1107 5 0.11927333149235864 1107 6 0.1510510897560664 1107 7 0.19883851590366675 1107 8 0.010956119155110855 1107 9 0.20295040678375642 1107 10-11 0.19420692274714924 1107 12-13 0.21820433795019056 1107 14-15 0.2288975043475645 1107 16-17 0.38090677060393574 1107 18-19 0.3038812476351751 1107 20-21 0.3943446956375425 1107 22-23 0.4797023332752346 1107 24-25 0.41521454003841285 1107 26-27 0.4092696448076296 1107 28-29 0.4878639363795614 1107 30-31 0.3384732651422766 1107 32-33 0.30423416762302224 1107 34-35 0.3808290143221953 1107 36-37 0.3197832069078572 1107 38-39 0.3099325278988232 1107 40-41 0.4534094382174274 1107 42-43 0.3744975537211346 1107 44-45 0.43616683747097795 1107 46-47 0.6214625649373176 1107 48-49 0.3849563003616865 1107 50-51 0.31895504341420633 1107 52-53 0.22220458651471375 1107 54-55 0.27599753388764015 1107 56-57 -0.21349216185718944 1107 58-59 0.0924876712306073 1107 60-61 0.08568930894637106 1107 62-63 -0.12650156637364063 1107 64-65 0.14831877170166763 1107 66-67 -0.1676923389714986 1107 68-69 -0.2572871275683113 1107 70-71 -0.1458585751023591 1107 72-73 0.11814534208781424 1107 74-75 -0.14644013669306588 1107 76-77 -0.3040826611502716 1107 78-79 0.13753231524094645 1107 80-81 0.00794903202812236 1107 82-83 -0.11898707073894244 1107 84-85 0.13884097609371437 1107 86-87 -0.07201194711614178 1107 88-89 -0.040203126281625146 1107 90-91 -0.1775039363945723 1107 92-93 0.2452509425717544 1107 94-95 0.0662364612373807 1107 96-97 -0.07824010858665886 1107 98-99 -0.18477821872634337 1107 100-101 -0.06150154488117465 1108 1 0.1725747963185853 1108 2 0.13654218696495235 1108 3 0.15166769504153876 1108 4 0.08466223109255822 1108 5 0.20036023868546238 1108 6 0.19559386261742873 1108 7 0.22754284991773233 1108 8 0.18877549741696953 1108 9 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-1.393408601732503 2316 72-73 -1.110873907702949 2316 74-75 -1.2695779286735842 2316 76-77 -1.054980477478356 2316 78-79 -1.1002862954263293 2316 80-81 -0.9961444017388992 2316 82-83 -1.137658606917899 2316 84-85 -1.0778485164118017 2316 86-87 -0.737476452936729 2316 88-89 -1.3246701429444876 2316 90-91 -1.1615296535063422 2316 92-93 -0.9309759462664111 2316 94-95 -0.8183941406039033 2316 96-97 -0.4804694321809677 2316 98-99 -0.6934596461021343 2316 100-101 -0.36383738905677987 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 1.0 5 11.0 6 11.0 7 50.0 8 113.0 9 191.0 10 439.0 11 698.0 12 1275.0 13 2034.0 14 2417.0 15 3720.0 16 5452.0 17 8140.0 18 7413.0 19 4145.0 20 3088.0 21 3090.0 22 3038.0 23 3072.0 24 3471.0 25 4017.0 26 4639.0 27 5781.0 28 7162.0 29 8972.0 30 11337.0 31 14483.0 32 19236.0 33 26321.0 34 38376.0 35 58801.0 36 93361.0 37 138664.0 38 108571.0 39 10064.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74140714227528 19.55605577232231 15.428159137646983 21.274377947755426 2 15.769397615592887 21.021874516960604 39.93873606145674 23.269991805989772 3 19.515871947398775 25.380157198678987 30.494848410810775 24.609122443111463 4 13.071567354821767 17.291912169225988 36.75133838715414 32.8851820887981 5 13.42697030802453 38.187295006342026 33.73108821813237 14.654646467501076 6 31.615084291932803 36.67442853842459 16.827472039809926 14.88301512983268 7 28.11974889430724 34.214397191764775 20.222085932396922 17.443767981531067 8 27.09491589089501 34.42913677016383 19.202405349209435 19.273541989731726 9 27.34671965349143 16.655946308457118 20.255161146097706 35.74217289195375 10-11 25.132425510131156 27.15923804015231 26.244849657364057 21.46348679235248 12-13 26.33215683960598 24.660327950050405 27.52654590361616 21.480969306727456 14-15 23.161126152854024 25.872462640798194 26.2154309999518 24.75098020639598 16-17 21.57052416734396 29.097877701488716 27.290957170091364 22.040640961075958 18-19 21.428417093403407 26.753692706333652 31.016406303238213 20.801483897024728 20-21 22.341309612255447 25.62232094924201 31.433336823682517 20.603032614820023 22-23 22.23567590926656 25.480572257241512 31.31890917099156 20.964842662500363 24-25 21.847757616856057 25.79708771912329 31.517437618318684 20.837717045701968 26-27 21.28811361993169 26.15186444058472 31.32401459308075 21.23600734640284 28-29 21.242595387366784 26.298840525752425 31.428466954319106 21.030097132561682 30-31 21.30018831264886 26.246761039060328 31.167904078556123 21.285146569734685 32-33 21.26860896288429 26.13872642144471 31.172059256156725 21.420605359514276 34-35 21.768393724684582 26.189419588172036 31.002029388740134 21.040157298403248 36-37 21.977648468650848 25.99113451307136 30.847622989101804 21.183594029175996 38-39 21.41422418199028 26.345094434799808 31.0323879742412 21.20829340896871 40-41 21.575096567450952 26.39532487218525 30.952111852034065 21.07746670832973 42-43 21.64015416288837 26.529265432288597 30.4593884517468 21.371191953076234 44-45 21.666591873469855 26.575601794371895 30.240967059414054 21.516839272744196 46-47 22.166459738822155 27.063502748234463 29.51264752958071 21.257389983362668 48-49 22.15906352269309 27.09292140564672 29.06796042276439 21.6800546488958 50-51 22.230532577423425 27.397412820218765 28.872002247120047 21.500052355237766 52-53 22.444607327406388 27.300846492780796 28.733967247228083 21.520578932584737 54-55 22.26867710779694 27.57425717890034 28.595433626024043 21.561632087278674 56-57 22.23984017524877 27.911242082309084 28.277894953786113 21.571022788656034 58-59 22.021277833457155 27.34181654392272 28.510834210075807 22.126071412544317 60-61 21.91473907977775 27.857390980605295 28.633411949293535 21.594457990323424 62-63 22.152082824324076 27.61206929506581 28.98036927894372 21.255478601666393 64-65 21.961858793768563 27.659355216160648 28.794715943748866 21.58407004632192 66-67 21.88232869449306 27.841518202170995 28.573328081188848 21.702825022147096 68-69 22.24783660498439 27.528489998894724 28.493323039564807 21.730350356556087 70-71 22.28961920290397 27.73348358455537 28.179583451755896 21.797313760784764 72-73 22.21673738778943 27.76871949060847 28.076285736604955 21.93825738499715 74-75 22.21175117466871 28.160386531241116 27.658191766432484 21.96967052765769 76-77 22.166709049478193 27.69833078205429 28.295596010364676 21.839364158102846 78-79 22.010889889455658 27.712209075240295 28.223960748530313 22.052940286773737 80-81 22.2843005755752 28.232271103731517 27.677139376291223 21.80628894440206 82-83 22.281817396102728 28.201684694806968 27.569176201525135 21.94732170756517 84-85 21.85917913499031 28.28666784119882 27.779070501846565 22.075082521964305 86-87 22.10620966361344 28.254459752118727 27.569935794195715 22.069394790072117 88-89 21.926539784163452 28.088668165854745 27.47220601702958 22.51258603295222 90-91 21.75252976499678 27.997922164273692 27.83211087331436 22.417437197415172 92-93 21.966314218351744 27.68593289935901 27.93233923129535 22.415413650993894 94-95 21.671661190142586 27.506610887562555 28.13961064323811 22.68211727905674 96-97 21.891885602974444 27.3582710472211 28.013043933523807 22.73679941628065 98-99 21.605923734412592 27.803738900269675 27.589493848141977 23.000843517175753 100-101 21.565089170534492 27.98365539345418 27.807691540435947 22.643563895575387 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 281.0 1 199.5 2 140.0 3 171.5 4 161.5 5 181.0 6 324.5 7 682.5 8 1073.5 9 1241.5 10 1189.0 11 1053.5 12 1067.5 13 1127.0 14 1174.5 15 1265.5 16 1351.5 17 1381.0 18 1400.5 19 1504.5 20 1596.5 21 1749.0 22 2025.5 23 2345.5 24 2682.5 25 3161.5 26 3799.0 27 4591.0 28 5268.5 29 6205.5 30 7354.5 31 8657.5 32 10485.5 33 11526.0 34 12279.0 35 13623.0 36 15644.0 37 17258.0 38 18291.5 39 19084.5 40 19139.0 41 19313.5 42 19003.0 43 19301.0 44 19988.5 45 20289.0 46 20545.0 47 20754.0 48 21886.5 49 21367.5 50 20113.5 51 19518.5 52 18795.5 53 18813.0 54 19210.0 55 18264.5 56 16072.0 57 14759.5 58 14241.5 59 12871.0 60 10312.5 61 8255.5 62 6698.5 63 4861.0 64 3304.0 65 2358.0 66 1768.5 67 1409.5 68 1086.5 69 859.0 70 610.0 71 368.0 72 273.5 73 217.5 74 153.0 75 123.0 76 96.5 77 58.0 78 42.5 79 31.0 80 22.5 81 16.0 82 8.5 83 6.0 84 6.0 85 4.5 86 1.5 87 0.5 88 0.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023601408771413708 2 0.0 3 0.0 4 3.324142080480804E-4 5 0.019280024066788665 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0020775888003005025 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010803461761562613 24-25 0.0 26-27 6.648284160961608E-4 28-29 0.0011634497281682815 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.31035520120201E-5 40-41 0.0011634497281682815 42-43 0.014543121602103515 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.31035520120201E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0011634497281682815 84-85 1.662071040240402E-4 86-87 0.0 88-89 0.0 90-91 0.011883807937718874 92-93 0.001745174592252422 94-95 0.0 96-97 0.0 98-99 0.001911381696276462 100-101 0.019113816962764624 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 601659.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.82090442989738 #Duplication Level Percentage of deduplicated Percentage of total 1 79.58107722812773 46.81030938063984 2 11.651957453581087 13.70757351596647 3 3.7304600070363514 6.582870946603187 4 1.646405419774915 3.873722233977814 5 0.8780102447531773 2.582267834754872 6 0.5554533240098283 1.9603360132110563 7 0.3445096732297338 1.4185059394954944 8 0.2591245586620099 1.219355272039794 9 0.19269454750417023 1.0201020806614607 >10 1.0414578769128662 11.200224399418994 >50 0.08008815318297706 3.232218089047777 >100 0.03648142421197266 3.983607161060377 >500 0.001425055633279695 0.6679021517406567 >1k 8.55033379967817E-4 1.741004981382209 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4376 0.7273222872091999 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3574 0.5940241897819196 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2435 0.4047142982985379 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 986 0.16388020456770364 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 974 0.16188571931941514 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 806 0.13396292584337638 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 646 0.10736978919952997 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 1.662071040240402E-4 0.0 0.0 0.0 0.0 14-15 1.662071040240402E-4 0.0 0.0 0.0 0.0 16-17 1.662071040240402E-4 0.0 0.0 0.0 0.0 18-19 1.662071040240402E-4 0.0 0.0 0.0 0.0 20-21 1.662071040240402E-4 0.0 0.0 0.0 0.0 22-23 1.662071040240402E-4 0.0 0.0 1.662071040240402E-4 0.0 24-25 3.324142080480804E-4 0.0 0.0 2.493106560360603E-4 0.0 26-27 3.324142080480804E-4 0.0 0.0 4.986213120721206E-4 0.0 28-29 4.986213120721206E-4 0.0 0.0 8.31035520120201E-4 0.0 30-31 4.986213120721206E-4 0.0 0.0 0.002576210112372623 0.0 32-33 4.986213120721206E-4 0.0 0.0 0.004487591808649085 0.0 34-35 5.817248640841407E-4 0.0 0.0 0.006565180608949587 0.0 36-37 6.648284160961608E-4 0.0 0.0 0.010304840449490493 0.0 38-39 8.31035520120201E-4 0.0 0.0 0.013296568321923215 0.0 40-41 8.31035520120201E-4 0.0 0.0 0.020775888003005026 0.0 42-43 8.31035520120201E-4 0.0 0.0 0.0318286604206037 0.0 44-45 8.31035520120201E-4 0.0 0.0 0.05260454842360872 0.0 46-47 9.972426241442412E-4 0.0 0.0 0.09623391322991928 0.0 48-49 9.972426241442412E-4 0.0 0.0 0.12847809141058308 0.0 50-51 9.972426241442412E-4 0.0 0.0 0.16678882888812432 0.0 52-53 9.972426241442412E-4 0.0 0.0 0.210916815006507 0.0 54-55 0.0011634497281682813 0.0 0.0 0.2692555085189451 0.0 56-57 0.0011634497281682813 0.0 0.0 0.3355721430245372 0.0 58-59 0.0011634497281682813 0.0 0.0 0.425905704061603 0.0 60-61 0.0011634497281682813 0.0 0.0 0.5390927419019744 0.0 62-63 0.0011634497281682813 0.0 0.0 0.6691498008007859 0.0 64-65 0.0011634497281682813 0.0 0.0 0.7831678741612774 0.0 66-67 0.0011634497281682813 0.0 0.0 0.9311753002946852 0.0 68-69 0.0011634497281682813 0.0 0.0 1.1046124133437711 0.0 70-71 0.0012465532801803013 0.0 0.0 1.260847091126369 0.0 72-73 0.0013296568321923216 0.0 0.0 1.4842294389346788 0.0 74-75 0.0013296568321923216 0.0 0.0 1.6771127831545776 0.0 76-77 0.0013296568321923216 0.0 0.0 1.8739850978710533 0.0 78-79 0.0013296568321923216 0.0 0.0 2.1357612867089166 0.0 80-81 0.0014958639362163619 0.0 0.0 2.3421074063547627 0.0 82-83 0.0014958639362163619 0.0 0.0 2.581694946805416 0.0 84-85 0.0014958639362163619 0.0 0.0 2.9589019693879752 0.0 86-87 0.0014958639362163619 0.0 0.0 3.3795721496728213 0.0 88-89 0.0014958639362163619 0.0 0.0 3.8982214177798387 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2420 0.0 45.962723 1 GGTAAGG 265 0.0 37.659134 3 GTATCAA 4090 0.0 37.07427 1 TAAGGTG 300 0.0 34.849644 5 TAGGCCG 55 0.0025727388 34.56163 4 GTAAGGT 320 0.0 32.671543 4 TATCAAC 5135 0.0 29.244457 2 ATCAACG 5155 0.0 29.223183 3 TCAACGC 5165 0.0 29.166603 4 CAACGCA 5235 0.0 28.7766 5 AGGTAAG 335 0.0 28.371487 2 AACGCAG 5345 0.0 28.25919 6 AAGGTGT 405 0.0 26.974483 6 GGTGTGC 425 0.0 25.705097 8 ACGCAGA 5945 0.0 25.247334 7 CGCAGAG 6075 0.0 24.550688 8 GGCGACA 175 1.4841316E-7 24.427824 6 CGATTCG 100 0.0016530277 23.749271 9 GTGCTAT 225 2.7830538E-9 23.238892 1 GAGTACT 3885 0.0 23.107403 12-13 >>END_MODULE