FastQCFastQC Report
Thu 26 May 2016
SRR937701_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937701_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544575
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31820.5843088647110132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25600.47009135564430976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16300.29931598035165036No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7360.13515126474773906No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6670.12248083367763851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16200.043.7358741
GTATCAA29450.041.1738431
AGTTGCG600.00393700531.6865675
TATCAAC38100.031.683652
ATCAACG37950.031.5526033
TCAACGC38100.031.4341724
CAACGCA38400.031.1914655
GTAAGGT1701.07320375E-1030.7517744
AACGCAG40100.029.855386
TGTACCG804.4740597E-429.7061585
GTACATG28950.027.7590561
ACATGGG27800.027.1768263
CGTAATA350.00839204627.1424178-79
ACGCAGA44400.026.8594557
GGTAAGG1955.311449E-1026.8043023
CGCAGAG44400.026.7524458
TACATGG28700.026.6606312
GCAGAGT47950.024.47239
CATGGGG11150.023.8692954
AGGTAAG2002.0372681E-823.7627352
Produced by FastQC (version 0.11.5)