FastQCFastQC Report
Thu 26 May 2016
SRR937701_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937701_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544575
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40620.7459027682137447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33780.6203002341275307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22690.41665518982692923No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC9730.17867144103199742No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8680.15939035027314877No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA7410.13606941192673186No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA5770.10595418445576826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25400.042.0958941
GTATCAA39600.033.6011241
AGGTAAG2500.030.3998552
TAAGGTG3600.030.3470735
GTAAGGT3300.028.7877394
ATCAACG49100.027.1841873
CTTACGC350.008395337527.14023468-69
TATCAAC49500.027.0604742
TCAACGC49650.026.8830534
CAACGCA50400.026.4830075
AGCCGCG1806.670234E-926.3936087
GCCGCGC1806.670234E-926.3936088
AACGCAG51700.025.814726
GGTAAGG3450.024.7824883
GGGCTAT1000.001648166223.7607961
GAGTACT35450.023.38570612-13
ACTGTAC3050.023.3648348
ACGCAGA57300.023.215327
CGCAGAG57950.023.0369078
GTGTGCA4800.022.7644869
Produced by FastQC (version 0.11.5)