##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937701_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 544575 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51242895836202 34.0 31.0 34.0 31.0 34.0 2 32.715701234907954 34.0 31.0 34.0 31.0 34.0 3 32.76705504292338 34.0 31.0 34.0 31.0 34.0 4 36.303324610935135 37.0 37.0 37.0 35.0 37.0 5 36.25209199834733 37.0 37.0 37.0 35.0 37.0 6 36.31547904329064 37.0 37.0 37.0 35.0 37.0 7 36.279752100261675 37.0 37.0 37.0 35.0 37.0 8 36.2480374604049 37.0 37.0 37.0 35.0 37.0 9 37.983471514483774 39.0 38.0 39.0 35.0 39.0 10-11 37.918454758297756 39.0 38.0 39.0 35.0 39.0 12-13 37.83104806500482 39.0 38.0 39.0 35.0 39.0 14-15 39.10834595785704 40.0 38.0 41.0 36.0 41.0 16-17 39.08065831152734 40.0 38.0 41.0 36.0 41.0 18-19 39.07902033696001 40.0 38.5 41.0 36.0 41.0 20-21 38.986426112105775 40.0 38.0 41.0 35.5 41.0 22-23 38.92890143690033 40.0 38.0 41.0 35.0 41.0 24-25 38.92442730569711 40.0 38.0 41.0 35.0 41.0 26-27 38.748806867740896 40.0 38.0 41.0 35.0 41.0 28-29 38.709119955928934 40.0 38.0 41.0 35.0 41.0 30-31 38.68909608410228 40.0 38.0 41.0 35.0 41.0 32-33 38.51730799247119 40.0 38.0 41.0 35.0 41.0 34-35 38.35950970940642 40.0 38.0 41.0 34.5 41.0 36-37 38.17725290364045 40.0 38.0 41.0 34.0 41.0 38-39 37.99833815360602 40.0 37.5 41.0 34.0 41.0 40-41 37.77865491438278 40.0 37.0 41.0 33.0 41.0 42-43 37.509722260478355 40.0 37.0 41.0 33.0 41.0 44-45 37.12496350364964 40.0 37.0 41.0 32.5 41.0 46-47 36.66331175687463 40.0 36.0 41.0 31.0 41.0 48-49 36.219015746224116 40.0 36.0 41.0 30.0 41.0 50-51 35.91141991461231 39.0 35.5 41.0 29.0 41.0 52-53 35.61962172336226 39.0 35.0 41.0 28.0 41.0 54-55 35.37114355231144 39.0 35.0 40.0 27.5 41.0 56-57 35.07862736996741 38.5 35.0 40.0 26.5 41.0 58-59 34.79783133636322 38.0 35.0 40.0 26.0 41.0 60-61 34.43952256346692 38.0 34.0 40.0 26.0 41.0 62-63 34.01609144745903 37.0 34.0 40.0 24.5 41.0 64-65 33.61536335674609 37.0 33.5 39.5 23.0 41.0 66-67 33.19887067896984 36.0 33.0 39.0 22.0 40.5 68-69 32.73018776109811 35.5 33.0 39.0 20.5 40.0 70-71 32.29578478630124 35.0 32.0 38.0 19.0 40.0 72-73 31.837133544507182 35.0 32.0 37.5 17.5 39.5 74-75 31.361335904145434 35.0 31.0 37.0 14.0 39.0 76-77 30.1394537024285 33.5 29.5 35.5 12.0 38.0 78-79 30.703891107744575 34.0 31.0 36.0 9.0 38.0 80-81 30.537516411880823 34.0 31.0 35.5 7.0 37.0 82-83 30.159998163705644 34.0 31.0 35.0 3.5 37.0 84-85 29.777688105403296 34.0 30.0 35.0 2.0 36.5 86-87 29.472995455171464 34.0 30.0 35.0 2.0 36.0 88-89 29.088909700224946 34.0 29.5 35.0 2.0 36.0 90-91 28.617394298306017 34.0 29.0 35.0 2.0 35.0 92-93 28.20335399164486 34.0 29.0 35.0 2.0 35.0 94-95 27.75459486755727 34.0 28.0 35.0 2.0 35.0 96-97 27.290242849928845 33.0 27.0 35.0 2.0 35.0 98-99 26.53449019877886 33.0 25.5 35.0 2.0 35.0 100-101 24.189084148188954 31.0 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2083486846158209 1101 2 0.19160081809967977 1101 3 0.15016582619515617 1101 4 0.09305865034092164 1101 5 0.06059551252655382 1101 6 0.11278905122439653 1101 7 0.11589481372467958 1101 8 0.12285758479791298 1101 9 0.07582725820715552 1101 10-11 0.17711016013004155 1101 12-13 0.16090157558580387 1101 14-15 0.22719682101617167 1101 16-17 0.25017010716721444 1101 18-19 0.2121926290554086 1101 20-21 0.3364634110279141 1101 22-23 0.36466756926023436 1101 24-25 0.17222136654051212 1101 26-27 0.2272284982665056 1101 28-29 0.18747168586720164 1101 30-31 0.19208182020587117 1101 32-33 0.28065708493409147 1101 34-35 0.20232067532309372 1101 36-37 0.0033635941233640665 1101 38-39 0.24305536676504857 1101 40-41 0.3667484636264007 1101 42-43 0.2573478712031658 1101 44-45 0.10047325177800559 1101 46-47 0.49205180024773654 1101 48-49 0.7767443727813514 1101 50-51 0.5090610895044492 1101 52-53 0.7275886124107203 1101 54-55 0.658133523110827 1101 56-57 0.6998895687375395 1101 58-59 0.6721046704713984 1101 60-61 0.7822388499878272 1101 62-63 0.7476894947411381 1101 64-65 0.6824614314784156 1101 66-67 0.8739320514025763 1101 68-69 0.7687118613049719 1101 70-71 0.7650611093751536 1101 72-73 0.4425605741434424 1101 74-75 0.656889277479447 1101 76-77 0.5592798421430167 1101 78-79 0.7126096641664503 1101 80-81 0.8104102923546854 1101 82-83 0.7616619349417135 1101 84-85 0.7682914627166504 1101 86-87 0.7471839592139986 1101 88-89 0.8351215256433377 1101 90-91 0.949810298466172 1101 92-93 1.15259940870056 1101 94-95 1.2665413136137609 1101 96-97 1.2730903554973843 1101 98-99 0.8501210477439791 1101 100-101 1.185641176184145 1102 1 -0.0840056642866358 1102 2 0.06702947335998743 1102 3 0.056084019516390526 1102 4 0.07125912346629804 1102 5 0.0257048081565614 1102 6 0.04574461778286576 1102 7 0.03324457807890013 1102 8 0.09479327275521854 1102 9 0.06413207565044843 1102 10-11 0.03789806059032941 1102 12-13 0.15987578291741045 1102 14-15 -0.012404277441589784 1102 16-17 -0.051010643231634845 1102 18-19 0.06149623617795896 1102 20-21 0.048332577190279835 1102 22-23 0.042145746810618334 1102 24-25 0.05828388144029617 1102 26-27 0.0550981962364645 1102 28-29 0.19250093528496137 1102 30-31 0.2385347552336512 1102 32-33 0.27443699911135155 1102 34-35 0.22693053026799248 1102 36-37 0.3153822165583122 1102 38-39 0.3501802131826892 1102 40-41 0.4499912521320155 1102 42-43 0.4555974906856619 1102 44-45 0.28731422296871045 1102 46-47 0.11012569399624539 1102 48-49 0.23859520075075835 1102 50-51 0.1812933977327944 1102 52-53 0.4234038367785118 1102 54-55 0.2819014257717001 1102 56-57 0.320857458561683 1102 58-59 0.23357887780300501 1102 60-61 0.08958008708232512 1102 62-63 0.006573203090511015 1102 64-65 0.1335752474729901 1102 66-67 0.19754041178474324 1102 68-69 0.11076042083991666 1102 70-71 0.1236369653103111 1102 72-73 -0.14403351912461204 1102 74-75 -0.009393433272233409 1102 76-77 0.06430450701254031 1102 78-79 -0.13295065931927397 1102 80-81 0.003685676998280485 1102 82-83 0.20864392683623478 1102 84-85 0.3176303708606376 1102 86-87 -0.010259054851395888 1102 88-89 -0.02820621454114658 1102 90-91 -0.22348791722765426 1102 92-93 -0.313572355983748 1102 94-95 0.027368870416310642 1102 96-97 0.09536232006799494 1102 98-99 0.43107041599322926 1102 100-101 0.7997563125286113 1103 1 0.16719759376593402 1103 2 0.18539355662133516 1103 3 0.1597107626979266 1103 4 0.08552852985899051 1103 5 0.13046281959265826 1103 6 0.08806176369590446 1103 7 0.015825618022958565 1103 8 0.08459643207892498 1103 9 0.21138977855883212 1103 10-11 0.163647525808976 1103 12-13 0.26514286506805007 1103 14-15 0.21016246738543032 1103 16-17 0.23302381931309668 1103 18-19 0.25779056132502376 1103 20-21 0.3501600896342296 1103 22-23 0.2542495458151066 1103 24-25 0.15636741017451072 1103 26-27 0.008610784166862118 1103 28-29 0.06896387082324651 1103 30-31 0.1361961152237825 1103 32-33 0.1728542519806595 1103 34-35 0.20102834383497736 1103 36-37 0.0814835062041297 1103 38-39 -0.007443249320914447 1103 40-41 -0.07902449306522641 1103 42-43 0.05295597931127105 1103 44-45 -0.24597334542159643 1103 46-47 -0.3704319184106808 1103 48-49 -0.48643333806202094 1103 50-51 -0.47940968548741125 1103 52-53 -0.7510930059546297 1103 54-55 -0.4996326768240493 1103 56-57 -0.3834200209726504 1103 58-59 -0.431424310316622 1103 60-61 -0.38982440302422106 1103 62-63 -0.6461968614946159 1103 64-65 -0.494303058915591 1103 66-67 -0.2888406610689316 1103 68-69 -0.2681450257025162 1103 70-71 -0.4015478942067432 1103 72-73 -0.09960810055535774 1103 74-75 -0.02562456166089433 1103 76-77 0.08671390270960799 1103 78-79 0.1304702958825068 1103 80-81 0.29416965412609386 1103 82-83 0.2708405412588739 1103 84-85 0.10381938521746292 1103 86-87 0.3265554994289097 1103 88-89 0.3110251401011688 1103 90-91 0.42888877453259866 1103 92-93 0.4964002878930067 1103 94-95 0.5853605093154854 1103 96-97 0.4555838429607526 1103 98-99 0.5650652841490604 1103 100-101 0.8946427229115947 1104 1 0.08388847819875167 1104 2 -0.07142979899234803 1104 3 0.006273050792472645 1104 4 0.06895146892708937 1104 5 0.0345180845350157 1104 6 0.01726717878841555 1104 7 0.09773615759478815 1104 8 0.06334534227944744 1104 9 -0.07329611701415928 1104 10-11 0.1594427161606191 1104 12-13 0.03790151081057047 1104 14-15 0.1600438003312803 1104 16-17 0.07932273920072674 1104 18-19 0.17934618953388082 1104 20-21 0.040378533884933177 1104 22-23 0.07676056489869865 1104 24-25 -0.09168911409169311 1104 26-27 -0.10047208902891214 1104 28-29 1.309249741652252E-4 1104 30-31 0.021210161528152582 1104 32-33 -0.0025429193842541054 1104 34-35 -0.024284670792553698 1104 36-37 -0.09056858621087116 1104 38-39 -0.03308683645644095 1104 40-41 0.0817239569977346 1104 42-43 0.26899661672288033 1104 44-45 0.18828147391386807 1104 46-47 0.2459949923710667 1104 48-49 0.027567557995325842 1104 50-51 0.2720161354948587 1104 52-53 0.03415897990219463 1104 54-55 0.09129898063986985 1104 56-57 0.4971107113726063 1104 58-59 0.28364761644885306 1104 60-61 0.13268903783599484 1104 62-63 0.11511888974048645 1104 64-65 0.33299096898328173 1104 66-67 0.15292166577403776 1104 68-69 0.06234877454934917 1104 70-71 0.17595198470443307 1104 72-73 0.026511971129231426 1104 74-75 0.06772887241834624 1104 76-77 -0.16270487090271857 1104 78-79 0.14066597543301995 1104 80-81 0.21186557585427224 1104 82-83 0.08583497117266248 1104 84-85 0.16206008559526097 1104 86-87 0.29842656404191104 1104 88-89 0.058784996731994 1104 90-91 0.4484446207013484 1104 92-93 0.1217151188529968 1104 94-95 0.13483437834580414 1104 96-97 0.28670125069106334 1104 98-99 0.6623268602412793 1104 100-101 0.7852843726258278 1105 1 -0.04369338195554917 1105 2 0.07479461021730316 1105 3 0.07333307144735102 1105 4 0.007138699350512923 1105 5 0.05416300282674058 1105 6 -0.008050238136434018 1105 7 -0.05354566003476435 1105 8 0.028080170054188613 1105 9 -0.100709396887801 1105 10-11 -0.06177333997205636 1105 12-13 -0.023994789535699113 1105 14-15 -0.10414124310472062 1105 16-17 0.0717062517455318 1105 18-19 0.07903712568599985 1105 20-21 0.11673704794848305 1105 22-23 0.07163101626860424 1105 24-25 0.18416745598302953 1105 26-27 -0.07141097488032244 1105 28-29 0.018860801652380133 1105 30-31 0.11180307811887502 1105 32-33 -0.16217781398361097 1105 34-35 0.0722920863911014 1105 36-37 0.010408723794597563 1105 38-39 -0.01885243392924707 1105 40-41 0.3433157497199275 1105 42-43 0.15035634577943569 1105 44-45 0.2930784182064343 1105 46-47 0.6048887412320099 1105 48-49 0.4102827636328925 1105 50-51 0.26407691552036994 1105 52-53 0.25510545945993357 1105 54-55 0.07114148717045765 1105 56-57 0.026122466430003044 1105 58-59 0.11220830534989545 1105 60-61 -0.15836253861743899 1105 62-63 0.0697616817944322 1105 64-65 -0.09695709006538067 1105 66-67 -0.11433882872811552 1105 68-69 0.09494475899743549 1105 70-71 -0.022410812209486153 1105 72-73 0.01585800113955571 1105 74-75 -0.0767869528656604 1105 76-77 -0.038228836639913055 1105 78-79 0.11443731374782473 1105 80-81 0.13109132155623726 1105 82-83 0.17830733008159783 1105 84-85 0.4604244012504459 1105 86-87 0.37282210501754776 1105 88-89 0.34280480111811684 1105 90-91 0.33675638790868945 1105 92-93 0.3448599763950675 1105 94-95 0.7802614483901529 1105 96-97 0.45761659605282645 1105 98-99 0.8010221096185965 1105 100-101 0.9425842025268061 1106 1 -0.2409963144279743 1106 2 -0.11875625645705412 1106 3 -0.16017417325844008 1106 4 -0.06271410052649884 1106 5 -0.15899372879246698 1106 6 -0.14789996333725242 1106 7 -0.08597388556226093 1106 8 -0.1569384701702603 1106 9 -0.22785686604495226 1106 10-11 -0.3588807422550033 1106 12-13 -0.42422635108687956 1106 14-15 -0.37296402645534954 1106 16-17 -0.41837311333383553 1106 18-19 -0.3536250634834559 1106 20-21 -0.34488955184398407 1106 22-23 -0.3858976166534873 1106 24-25 -0.4876733142233576 1106 26-27 -0.37334898172147746 1106 28-29 -0.7471443292135262 1106 30-31 -0.6043658753382104 1106 32-33 -0.6230717760882243 1106 34-35 -0.6766509936657883 1106 36-37 -0.4586835741415243 1106 38-39 -0.46897301888639475 1106 40-41 -0.6570661827140682 1106 42-43 -0.5640807002254036 1106 44-45 -0.6297640238551381 1106 46-47 -0.6852646082433722 1106 48-49 -0.9397354922570358 1106 50-51 -0.8160352140772247 1106 52-53 -0.609118761301815 1106 54-55 -0.49351559034982273 1106 56-57 -0.8488222854820577 1106 58-59 -0.8267137323917595 1106 60-61 -0.8065619313688899 1106 62-63 -0.8735249548941368 1106 64-65 -1.3131212393409157 1106 66-67 -1.5375728115867986 1106 68-69 -1.602562351191267 1106 70-71 -1.5440392375909404 1106 72-73 -1.5070627490814346 1106 74-75 -2.074159065552543 1106 76-77 -1.8272868327197003 1106 78-79 -1.7656503145238212 1106 80-81 -1.6904093125957296 1106 82-83 -1.9005412997658233 1106 84-85 -1.6641047889567098 1106 86-87 -1.7271385006936626 1106 88-89 -1.8746324562707137 1106 90-91 -2.1953451532727577 1106 92-93 -1.9011069131856217 1106 94-95 -1.8285004179382973 1106 96-97 -2.670580586775106 1106 98-99 -2.7305485695005096 1106 100-101 -2.013541609379878 1107 1 0.1418393056957825 1107 2 0.22007306058049636 1107 3 0.15474953633839306 1107 4 0.018034583127757742 1107 5 0.07867874132552544 1107 6 0.05429843014443492 1107 7 -0.03478052686285338 1107 8 0.10556201024789402 1107 9 0.20982329076353068 1107 10-11 0.14630777382938476 1107 12-13 0.21480666324880104 1107 14-15 0.21547134769431153 1107 16-17 0.3050597626414131 1107 18-19 0.311331314547985 1107 20-21 0.23432179371118877 1107 22-23 0.32087435767296313 1107 24-25 0.2159471169999776 1107 26-27 0.26666408565236566 1107 28-29 0.4248838040736871 1107 30-31 0.3164035623803798 1107 32-33 0.16590935308660448 1107 34-35 0.2472315537034433 1107 36-37 0.35256974074375336 1107 38-39 0.49734006001747844 1107 40-41 0.27536780783130865 1107 42-43 0.15746893657846783 1107 44-45 0.19486413249214962 1107 46-47 0.15694685261215824 1107 48-49 0.12366048760383563 1107 50-51 0.04600790825644907 1107 52-53 -0.12443449211391311 1107 54-55 -0.016479578204851464 1107 56-57 -0.03153491855788815 1107 58-59 0.15654849905449453 1107 60-61 0.36585053644309085 1107 62-63 0.2885873476540013 1107 64-65 0.5238056217990277 1107 66-67 0.6934698879789067 1107 68-69 0.6335343473751678 1107 70-71 0.43097901829898433 1107 72-73 0.3073228921807214 1107 74-75 0.27218399144426897 1107 76-77 0.36128690185887535 1107 78-79 0.29361406919576893 1107 80-81 0.4001591181664068 1107 82-83 0.4193787586530249 1107 84-85 0.336029728126956 1107 86-87 0.36041290404902426 1107 88-89 0.14062562436267 1107 90-91 0.5468048140588095 1107 92-93 0.5114761991553571 1107 94-95 0.468820770424049 1107 96-97 0.40086720137970744 1107 98-99 0.6846480176089109 1107 100-101 0.9509074470788654 1108 1 0.13975045751050885 1108 2 0.15923300487787628 1108 3 -0.021435085222535122 1108 4 0.008455990998889718 1108 5 0.11364969818109216 1108 6 0.17814893636079887 1108 7 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0.6436822559812185 2316 60-61 0.857449086908666 2316 62-63 1.1938516601814158 2316 64-65 0.831421365172929 2316 66-67 0.8262396037596531 2316 68-69 0.8145798972069755 2316 70-71 1.0952947957987007 2316 72-73 1.0987979397130943 2316 74-75 0.8617439309928265 2316 76-77 1.1377750309519392 2316 78-79 1.33335438802834 2316 80-81 1.1313046078717868 2316 82-83 1.1323156909051662 2316 84-85 1.3004738836756538 2316 86-87 1.307940407827239 2316 88-89 1.2917298380877433 2316 90-91 1.5576072581168567 2316 92-93 1.6424868106573811 2316 94-95 1.59210026081195 2316 96-97 1.8060055734160407 2316 98-99 1.8114091531858811 2316 100-101 1.8605471760224255 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 2.0 5 5.0 6 20.0 7 41.0 8 92.0 9 201.0 10 370.0 11 670.0 12 1199.0 13 1807.0 14 2385.0 15 3518.0 16 5095.0 17 7948.0 18 7031.0 19 4131.0 20 3008.0 21 2971.0 22 2932.0 23 3034.0 24 3396.0 25 3709.0 26 4520.0 27 5427.0 28 6488.0 29 8082.0 30 10030.0 31 12470.0 32 16882.0 33 22646.0 34 32723.0 35 51218.0 36 82312.0 37 127134.0 38 101992.0 39 9082.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.091096137029986 19.515273317804052 15.335961554005895 21.057668991160067 2 15.757058256438508 20.93485745765046 40.04774365330763 23.26034063260341 3 19.501813340678513 25.47472799889822 30.372675939953176 24.65078272047009 4 13.144562273332417 17.200752880686775 36.821190836891155 32.83349400908966 5 13.399899728016837 38.203074640187026 33.66787935774509 14.729146274051045 6 31.647431483266768 36.725152641968506 16.766469265023183 14.860946609741541 7 28.015057613735483 34.31721985034201 20.28756369646054 17.380158839461966 8 27.212229720424187 34.319239774135795 19.15695726024882 19.311573245191205 9 27.31487857503558 16.656842491851446 20.2038286737364 35.824450259376576 10-11 25.12821925354634 27.241426800716155 26.201625120506815 21.428728825230685 12-13 26.43094427906926 24.645994053838653 27.450863368671186 21.472198298420906 14-15 23.304136253041364 25.770187761098107 26.21879447275398 24.706881513106552 16-17 21.522563466923746 29.05596107055961 27.275030987467293 22.14644447504935 18-19 21.366294817059174 26.66345315154019 31.181655419363725 20.788596612036912 20-21 22.324606022322037 25.586605309838518 31.540800699262178 20.54798796857727 22-23 22.179425615467743 25.47214246954682 31.471604425913867 20.876827489071562 24-25 21.86769499150714 25.81040260753799 31.566542716797503 20.75535968415737 26-27 21.286650543824564 26.069423709392197 31.42531065852078 21.21861508826246 28-29 21.22207072585125 26.366520220174728 31.47479421742943 20.9366148365446 30-31 21.32865078272047 26.258550245604372 31.26373777716568 21.149061194509482 32-33 21.259422485424416 26.22815957397971 31.182757195978517 21.329660744617364 34-35 21.75586466510582 26.245879814534266 31.012073635403752 20.98618188495616 36-37 22.08704993366356 25.974686566067877 30.894417180448887 21.043846319819675 38-39 21.53119405040628 26.372492310517377 30.922370656016156 21.173942983060183 40-41 21.59143181119135 26.31513710295691 31.04885024491689 21.044580840934863 42-43 21.76476422423768 26.557053069290927 30.431181101205233 21.247001605266156 44-45 21.733002800348896 26.577790019740167 30.34136712114952 21.34784005876142 46-47 22.228894091722903 27.07882293531653 29.55203599136942 21.140246981591147 48-49 22.222283432034153 27.16466969655236 29.077262085112242 21.535784786301242 50-51 22.223936096956344 27.535784786301242 28.845154478262863 21.395124638479547 52-53 22.476702015333057 27.25657622916954 28.849469770004134 21.417251985493273 54-55 22.194188128356977 27.721801404765184 28.578524537483357 21.50548592939448 56-57 22.278290409952714 27.874397465913788 28.334297387871278 21.513014736262225 58-59 22.088417573337004 27.30496258550246 28.488637928659966 22.11798191250057 60-61 21.849056603773583 27.835743469678185 28.813662030023412 21.501537896524813 62-63 22.037460404902905 27.6174998852316 29.087361704081165 21.257678005784328 64-65 21.96823210760685 27.701510352109445 28.745994582931644 21.584262957352063 66-67 21.851719230592664 27.806087315796724 28.665840334205573 21.67635311940504 68-69 22.199605196713033 27.580131295046595 28.633062479915534 21.58720102832484 70-71 22.315291741266126 27.740256163062938 28.293623467841893 21.650828627829043 72-73 22.142404627461783 27.711977229949962 28.16811274847358 21.977505394114676 74-75 22.27755589220952 28.06711655878437 27.772207684891885 21.883119864114217 76-77 22.194096313639076 27.672496901253268 28.342193453610616 21.79121333149704 78-79 22.093559197539367 27.70472386723592 28.165174677500804 22.036542257723912 80-81 22.282697516411883 28.332277464077492 27.67359867786806 21.711426341642564 82-83 22.315465511510855 28.249380707453366 27.500904378292717 21.934249402743067 84-85 21.849699306798883 28.308589266859478 27.80480190974613 22.03690951659551 86-87 22.07785888077859 28.26938438231649 27.604645824725704 22.048110912179222 88-89 21.948124684386908 28.051875315613096 27.53192856814947 22.468071431850525 90-91 21.733552981968977 28.16148949690178 27.760024682923607 22.34493283820564 92-93 21.944454136282754 27.729777673309965 27.844641260175667 22.481126930231618 94-95 21.6947160629849 27.47904329063949 28.079878804572374 22.746361841803242 96-97 21.862828811458478 27.470596336592756 27.962264150943394 22.70431070100537 98-99 21.524361467509166 27.8897623625289 27.598981947191266 22.98689422277067 100-101 21.515775670416634 28.133210038320016 27.743306393923895 22.607707897339456 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 228.0 1 169.5 2 147.0 3 178.0 4 151.5 5 158.5 6 278.0 7 581.0 8 959.0 9 1169.5 10 1107.0 11 999.0 12 995.0 13 1052.5 14 1120.0 15 1152.5 16 1234.0 17 1312.5 18 1374.5 19 1475.5 20 1568.5 21 1672.5 22 1829.0 23 2099.5 24 2457.0 25 2902.0 26 3399.0 27 4122.5 28 4801.5 29 5626.0 30 6677.0 31 7752.0 32 9337.0 33 10409.5 34 11166.0 35 12339.5 36 14147.5 37 15589.0 38 16591.5 39 17212.5 40 17260.5 41 17596.0 42 17400.5 43 17379.5 44 17847.5 45 18353.0 46 18655.0 47 18807.5 48 19626.5 49 19403.5 50 18434.5 51 17741.0 52 16930.5 53 16856.0 54 17297.5 55 16429.0 56 14525.5 57 13348.0 58 12912.5 59 11684.0 60 9180.0 61 7281.0 62 5964.5 63 4379.5 64 3003.5 65 2164.5 66 1618.0 67 1293.0 68 1000.5 69 783.0 70 584.5 71 375.5 72 265.5 73 206.0 74 160.5 75 125.0 76 96.5 77 61.0 78 42.0 79 29.5 80 15.0 81 9.5 82 6.5 83 6.5 84 4.0 85 2.0 86 1.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04168388192627278 2 0.0 3 0.0 4 0.0 5 0.010283248404719277 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004039847587568287 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.8362943579855852E-4 22-23 0.0017444796400863057 24-25 0.0 26-27 0.001101776614791351 28-29 0.001377220768489189 30-31 0.0 32-33 0.0 34-35 0.0 36-37 4.5907358949639625E-4 38-39 0.0 40-41 4.5907358949639625E-4 42-43 0.021484643988431345 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.6725887159711704E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012670431070100538 92-93 0.0029380709727769364 94-95 0.0 96-97 0.0 98-99 0.001101776614791351 100-101 0.014965799017582519 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 544575.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.48174729558035 #Duplication Level Percentage of deduplicated Percentage of total 1 79.97631530363401 47.57130976522412 2 11.511671285249415 13.694686446779885 3 3.6490093156709884 6.511483499818809 4 1.5820508258331294 3.764125897238816 5 0.854040945524296 2.5399923850877335 6 0.5128486788972147 1.8303081311421818 7 0.35459923121102166 1.4764527303470738 8 0.24967684795106798 1.188097214030597 9 0.17920823178694906 0.9593656880795183 >10 1.0152358862691504 11.064841616648744 >50 0.07814596898202367 3.134041960425635 >100 0.0346968081342725 3.7773607863528667 >500 0.001562919285327577 0.6827362082835037 >1k 9.377515711965462E-4 1.8051976705404513 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4062 0.7459027682137447 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3378 0.6203002341275307 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2269 0.41665518982692923 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 973 0.17867144103199742 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 868 0.15939035027314877 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 741 0.13606941192673186 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 577 0.10595418445576826 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.8362943579855852E-4 3 0.0 0.0 0.0 0.0 1.8362943579855852E-4 4 0.0 0.0 0.0 0.0 1.8362943579855852E-4 5 0.0 0.0 0.0 0.0 1.8362943579855852E-4 6 0.0 0.0 0.0 0.0 1.8362943579855852E-4 7 0.0 0.0 0.0 0.0 1.8362943579855852E-4 8 0.0 0.0 0.0 0.0 1.8362943579855852E-4 9 0.0 0.0 0.0 0.0 1.8362943579855852E-4 10-11 1.8362943579855852E-4 0.0 0.0 0.0 1.8362943579855852E-4 12-13 1.8362943579855852E-4 0.0 0.0 0.0 1.8362943579855852E-4 14-15 1.8362943579855852E-4 0.0 0.0 0.0 1.8362943579855852E-4 16-17 1.8362943579855852E-4 0.0 0.0 0.0 1.8362943579855852E-4 18-19 2.754441536978378E-4 0.0 0.0 0.0 1.8362943579855852E-4 20-21 3.6725887159711704E-4 0.0 0.0 9.181471789927926E-5 1.8362943579855852E-4 22-23 3.6725887159711704E-4 0.0 0.0 1.8362943579855852E-4 1.8362943579855852E-4 24-25 7.345177431942341E-4 0.0 0.0 1.8362943579855852E-4 1.8362943579855852E-4 26-27 7.345177431942341E-4 0.0 0.0 2.754441536978378E-4 1.8362943579855852E-4 28-29 7.345177431942341E-4 0.0 0.0 7.345177431942341E-4 1.8362943579855852E-4 30-31 7.345177431942341E-4 0.0 0.0 0.003121700408575495 1.8362943579855852E-4 32-33 7.345177431942341E-4 0.0 0.0 0.005600697791856035 1.8362943579855852E-4 34-35 7.345177431942341E-4 0.0 0.0 0.00743699214984162 1.8362943579855852E-4 36-37 7.345177431942341E-4 0.0 0.0 0.009089657072028646 1.8362943579855852E-4 38-39 7.345177431942341E-4 0.0 0.0 0.011385025019510627 1.8362943579855852E-4 40-41 7.345177431942341E-4 0.0 0.0 0.017903869990359457 1.8362943579855852E-4 42-43 7.345177431942341E-4 0.0 0.0 0.026350824037093146 1.8362943579855852E-4 44-45 9.181471789927925E-4 0.0 0.0 0.043336546848459806 1.8362943579855852E-4 46-47 0.0010099618968920719 0.0 0.0 0.08749942615801312 1.8362943579855852E-4 48-49 0.0011017766147913511 0.0 0.0 0.1168801358857825 1.8362943579855852E-4 50-51 0.0011017766147913511 0.0 0.0 0.15342239360969562 1.8362943579855852E-4 52-53 0.0011017766147913511 0.0 0.0 0.19905430840563743 2.754441536978378E-4 54-55 0.0011017766147913511 0.0 0.0 0.26093742826975164 3.6725887159711704E-4 56-57 0.0011017766147913511 0.0 0.0 0.32906394895101687 3.6725887159711704E-4 58-59 0.0011017766147913511 0.0 0.0 0.42317403479777804 3.6725887159711704E-4 60-61 0.0011017766147913511 0.0 0.0 0.5407886884267548 5.508883073956756E-4 62-63 0.0011017766147913511 0.0 0.0 0.6816324656842492 5.508883073956756E-4 64-65 0.0011017766147913511 0.0 0.0 0.7964926777762475 5.508883073956756E-4 66-67 0.0011017766147913511 0.0 0.0 0.9654317587109214 5.508883073956756E-4 68-69 0.0011017766147913511 0.0 0.0 1.1687095441399258 5.508883073956756E-4 70-71 0.0011017766147913511 0.0 0.0 1.333516962769132 7.345177431942341E-4 72-73 0.0011017766147913511 0.0 0.0 1.5840793279162648 7.345177431942341E-4 74-75 0.0011017766147913511 0.0 0.0 1.7986503236468807 7.345177431942341E-4 76-77 0.0011017766147913511 0.0 0.0 2.0031217004085757 7.345177431942341E-4 78-79 0.0011017766147913511 0.0 0.0 2.2688334940090895 7.345177431942341E-4 80-81 0.0016526649221870265 0.0 0.0 2.484598081072396 7.345177431942341E-4 82-83 0.0016526649221870265 0.0 0.0 2.7448009915989533 7.345177431942341E-4 84-85 0.0016526649221870265 0.0 0.0 3.150438415277969 7.345177431942341E-4 86-87 0.0016526649221870265 0.0 0.0 3.598494238626452 7.345177431942341E-4 88-89 0.0016526649221870265 0.0 0.0 4.140660147821696 7.345177431942341E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2540 0.0 42.095894 1 GTATCAA 3960 0.0 33.601124 1 AGGTAAG 250 0.0 30.399855 2 TAAGGTG 360 0.0 30.347073 5 GTAAGGT 330 0.0 28.787739 4 ATCAACG 4910 0.0 27.184187 3 CTTACGC 35 0.0083953375 27.140234 68-69 TATCAAC 4950 0.0 27.060474 2 TCAACGC 4965 0.0 26.883053 4 CAACGCA 5040 0.0 26.483007 5 AGCCGCG 180 6.670234E-9 26.393608 7 GCCGCGC 180 6.670234E-9 26.393608 8 AACGCAG 5170 0.0 25.81472 6 GGTAAGG 345 0.0 24.782488 3 GGGCTAT 100 0.0016481662 23.760796 1 GAGTACT 3545 0.0 23.385706 12-13 ACTGTAC 305 0.0 23.364834 8 ACGCAGA 5730 0.0 23.21532 7 CGCAGAG 5795 0.0 23.036907 8 GTGTGCA 480 0.0 22.764486 9 >>END_MODULE