FastQCFastQC Report
Thu 26 May 2016
SRR937700_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937700_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415100
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26280.6331004577210311No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20420.49192965550469764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13240.31895928691881476No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5360.12912551192483737No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5330.12840279450734765No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCG250.00500869757.0296365
GGTATCA14900.045.3193631
GTATCAA24850.038.2723661
TATCAAC31800.029.8861392
AGGATAG804.475085E-429.7029385
ATCAACG32250.029.469123
TCAACGC32600.029.1562584
CAACGCA33200.028.4861895
AGCCGCG856.394314E-427.9455787
AACGCAG34000.027.8024966
TAGTACA1056.8709865E-527.1569714
TGGACCG908.9107576E-426.4026095
GTACATG21900.026.2738041
CATGGGG7950.025.7051814
GTGTAAG1701.1070915E-725.1753371
CGCAGAG37100.025.0982938
ACGCAGA37500.025.0839527
TACATGG22950.025.0535682
GCCGCGC950.001224105125.003948
GGATAGT1151.2794998E-424.7835226
Produced by FastQC (version 0.11.5)