##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937700_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 415100 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09249337509034 33.0 31.0 34.0 31.0 34.0 2 32.2108431703204 33.0 31.0 34.0 31.0 34.0 3 32.2520741989882 34.0 31.0 34.0 30.0 34.0 4 35.83138761744158 37.0 35.0 37.0 35.0 37.0 5 35.836668272705374 37.0 35.0 37.0 35.0 37.0 6 35.91137798120935 37.0 36.0 37.0 35.0 37.0 7 35.828238978559384 37.0 36.0 37.0 35.0 37.0 8 35.84607564442303 37.0 36.0 37.0 35.0 37.0 9 37.451279209828954 39.0 37.0 39.0 35.0 39.0 10-11 37.39945555287882 39.0 37.0 39.0 35.0 39.0 12-13 37.31568296795953 39.0 37.0 39.0 35.0 39.0 14-15 38.51290773307637 40.0 38.0 41.0 34.5 41.0 16-17 38.44622139243556 40.0 38.0 41.0 34.0 41.0 18-19 38.402065767284995 40.0 38.0 41.0 34.0 41.0 20-21 38.42341363526861 40.0 38.0 41.0 34.0 41.0 22-23 38.420801011804386 40.0 38.0 41.0 34.0 41.0 24-25 38.34784630209588 40.0 38.0 41.0 34.0 41.0 26-27 38.2283305227656 40.0 38.0 41.0 34.0 41.0 28-29 38.08353529270056 40.0 38.0 41.0 34.0 41.0 30-31 37.80506865815467 40.0 37.5 41.0 33.0 41.0 32-33 37.64585280655264 40.0 37.0 41.0 33.0 41.0 34-35 37.64466032281378 40.0 37.0 41.0 33.0 41.0 36-37 37.490679354372446 40.0 37.0 41.0 33.0 41.0 38-39 37.29938569019514 40.0 37.0 41.0 32.5 41.0 40-41 37.04385087930619 40.0 36.5 41.0 31.5 41.0 42-43 36.74541074439894 40.0 36.0 41.0 31.0 41.0 44-45 36.42823656950132 39.0 36.0 41.0 30.5 41.0 46-47 35.97611298482293 39.0 35.5 41.0 29.5 41.0 48-49 35.54558419657914 39.0 35.0 40.5 27.5 41.0 50-51 34.65120814261624 38.0 34.0 39.5 25.5 40.5 52-53 34.72076005781739 38.0 34.5 40.0 26.0 40.5 54-55 34.9541279209829 39.0 35.0 40.0 26.0 41.0 56-57 34.69225367381354 38.0 34.5 40.0 24.5 41.0 58-59 34.41218140207179 38.0 34.0 40.0 23.5 41.0 60-61 34.05989520597446 38.0 34.0 40.0 22.5 41.0 62-63 33.69222235605878 37.0 34.0 40.0 21.5 41.0 64-65 33.2461238255842 37.0 33.0 39.5 20.0 41.0 66-67 32.70161527342809 36.0 32.5 39.0 16.5 41.0 68-69 32.27933509997591 35.5 32.0 39.0 14.0 40.0 70-71 31.814425439653096 35.0 31.5 38.5 9.0 40.0 72-73 31.350309563960494 35.0 31.0 37.5 7.0 40.0 74-75 30.82997350036136 35.0 31.0 37.0 4.0 39.0 76-77 30.313930378222118 34.5 30.0 36.5 2.0 39.0 78-79 29.805667309082146 34.0 29.5 36.0 2.0 38.5 80-81 29.29356661045531 34.0 29.0 35.5 2.0 37.0 82-83 28.7857684895206 34.0 29.0 35.0 2.0 37.0 84-85 28.26091785112021 34.0 27.5 35.0 2.0 36.0 86-87 27.70852445193929 33.5 26.5 35.0 2.0 36.0 88-89 27.19760660081908 33.0 26.0 35.0 2.0 35.5 90-91 26.659878342568057 33.0 25.0 35.0 2.0 35.0 92-93 26.037557215128885 32.0 24.0 34.5 2.0 35.0 94-95 25.299424235124064 32.0 20.5 34.0 2.0 35.0 96-97 24.47160081907974 31.5 12.5 34.0 2.0 35.0 98-99 23.327920982895687 31.0 2.0 34.0 2.0 35.0 100-101 20.12868465430017 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.19512155071606685 1101 2 -0.2644884593192067 1101 3 -0.23648826521764477 1101 4 -0.27843147207120467 1101 5 -0.20832755361101363 1101 6 -0.1835681933625608 1101 7 -0.3020736011694467 1101 8 -0.28873258347328346 1101 9 0.1034020119641923 1101 10-11 -0.028019530340216647 1101 12-13 0.09196302551330149 1101 14-15 -0.03434796346360969 1101 16-17 0.045838909926182225 1101 18-19 -0.15414328609883654 1101 20-21 -0.09878863722551046 1101 22-23 -0.29349734484155476 1101 24-25 -0.1715689629561581 1101 26-27 -0.08113643658843728 1101 28-29 -0.0822046824761884 1101 30-31 -0.03558940991611337 1101 32-33 0.12469155106833085 1101 34-35 0.040978848827933234 1101 36-37 0.2744375820025695 1101 38-39 0.0884035127166598 1101 40-41 0.12937914403561734 1101 42-43 0.2558468966368679 1101 44-45 0.3893077108119698 1101 46-47 0.22856117301309098 1101 48-49 0.7769771619181043 1101 50-51 0.5327690904462443 1101 52-53 0.5765433693648063 1101 54-55 0.7902167464716072 1101 56-57 0.8044242439549976 1101 58-59 0.780121898444385 1101 60-61 0.8378250744859699 1101 62-63 0.5952994903825513 1101 64-65 0.751572104705545 1101 66-67 0.8200794276448278 1101 68-69 0.9774834671639354 1101 70-71 1.0389966897413636 1101 72-73 1.1273139528527878 1101 74-75 0.8226032518057877 1101 76-77 0.7351494005192087 1101 78-79 0.8012379173828492 1101 80-81 0.9280229228601122 1101 82-83 1.1199728854944873 1101 84-85 1.2839311113268543 1101 86-87 1.386288507870315 1101 88-89 1.6336974610010984 1101 90-91 1.8353992737600215 1101 92-93 1.9762682545290247 1101 94-95 1.840979398391358 1101 96-97 1.3414262689128513 1101 98-99 1.984867072601542 1101 100-101 2.7292910591253268 1102 1 0.17862338139462253 1102 2 0.2394910242322581 1102 3 0.16603819664734232 1102 4 0.22807855486684048 1102 5 0.1831107745977434 1102 6 0.20298591400866428 1102 7 0.13744505138422625 1102 8 0.20960079521028518 1102 9 0.5116766167368496 1102 10-11 0.38824465294731425 1102 12-13 0.3484254346994504 1102 14-15 0.5052700061154525 1102 16-17 0.6288502565561984 1102 18-19 0.38540325685237775 1102 20-21 0.39322858679560824 1102 22-23 0.339692908484885 1102 24-25 0.35329090480253456 1102 26-27 0.5577067241418803 1102 28-29 0.5328193985012035 1102 30-31 0.5331672225564716 1102 32-33 0.8101014718868171 1102 34-35 0.8873367710613493 1102 36-37 0.9495858772870491 1102 38-39 1.178224197868083 1102 40-41 0.8762227673383833 1102 42-43 1.1468124485946376 1102 44-45 1.0096309673219963 1102 46-47 1.3049536521293135 1102 48-49 1.3141734286863525 1102 50-51 1.1272393089436932 1102 52-53 1.2644192094780067 1102 54-55 1.249438944507034 1102 56-57 1.382479943119641 1102 58-59 1.1729207338502263 1102 60-61 1.334712914272636 1102 62-63 1.4418818963034852 1102 64-65 1.6055012494907288 1102 66-67 1.688538789294853 1102 68-69 1.7332928328954864 1102 70-71 1.5788459455439288 1102 72-73 1.751535062482457 1102 74-75 1.7626933173822366 1102 76-77 1.6481075513178247 1102 78-79 1.6819350412706378 1102 80-81 1.8402361959683269 1102 82-83 2.154039985709449 1102 84-85 2.2137935640492294 1102 86-87 2.1257003606090166 1102 88-89 2.2804587736184416 1102 90-91 2.100463489699724 1102 92-93 2.3218608914640733 1102 94-95 2.184830586114142 1102 96-97 2.343490838584426 1102 98-99 2.405685553731306 1102 100-101 3.0592276594899452 1103 1 -0.028621384215895773 1103 2 0.1328859054464573 1103 3 0.0530446656196375 1103 4 -0.018318396740738763 1103 5 0.07437887039976232 1103 6 0.15909276886361567 1103 7 0.2484447210880063 1103 8 0.17777554607832258 1103 9 0.019370101845325394 1103 10-11 0.1396081318526683 1103 12-13 0.2281674008481076 1103 14-15 0.35168629213585945 1103 16-17 0.4452619506535669 1103 18-19 0.5025496586375837 1103 20-21 0.48291408034677374 1103 22-23 0.3228880813264823 1103 24-25 0.38068132298977275 1103 26-27 0.39471560991577803 1103 28-29 0.2842332274399837 1103 30-31 0.03529803597133707 1103 32-33 0.6199354376165687 1103 34-35 0.5908999422272885 1103 36-37 0.5187023751804034 1103 38-39 0.6953834892618502 1103 40-41 0.6180136978875623 1103 42-43 0.8064683766061762 1103 44-45 0.8191930428712126 1103 46-47 1.3984637342200017 1103 48-49 1.5274440862980683 1103 50-51 1.3062799998267138 1103 52-53 1.3392715831799862 1103 54-55 1.5289037840716801 1103 56-57 1.5681044043742887 1103 58-59 1.6012283282464708 1103 60-61 1.3874656513004524 1103 62-63 1.5126357772840606 1103 64-65 1.3233698721554958 1103 66-67 1.4873722797202902 1103 68-69 1.9085270612510072 1103 70-71 1.923945074689751 1103 72-73 1.8796920095786724 1103 74-75 2.0510197628309896 1103 76-77 1.8835281956371368 1103 78-79 1.9828389250708156 1103 80-81 2.0051667934822426 1103 82-83 1.823993502558583 1103 84-85 1.98873898461299 1103 86-87 2.182056146029261 1103 88-89 2.297499238933309 1103 90-91 2.370796847201074 1103 92-93 2.825467967859172 1103 94-95 2.853056089815876 1103 96-97 2.5274920147612043 1103 98-99 2.5846658245742056 1103 100-101 3.2051702523958276 1104 1 0.017177827188405104 1104 2 0.0627167134273634 1104 3 0.11982474236924645 1104 4 0.03648768520464074 1104 5 0.09111345124400572 1104 6 0.23965348941326425 1104 7 0.11849786558036612 1104 8 0.11460876732029135 1104 9 0.4074567682482453 1104 10-11 0.055018715914592065 1104 12-13 0.08583115313939516 1104 14-15 0.22766709154507936 1104 16-17 0.4098278933419124 1104 18-19 0.3221550970466751 1104 20-21 -0.23219583512315722 1104 22-23 -0.0188455104763392 1104 24-25 -0.014124231466844606 1104 26-27 0.09735041626146312 1104 28-29 -0.22058010747581847 1104 30-31 0.07609269778509997 1104 32-33 0.29287262689272353 1104 34-35 0.46355096885228164 1104 36-37 0.23117484686085277 1104 38-39 0.5357258294657257 1104 40-41 0.7337842930267513 1104 42-43 0.8727143709884189 1104 44-45 0.9972204986610649 1104 46-47 0.9186863344758365 1104 48-49 0.9378237463315671 1104 50-51 1.1627503088032611 1104 52-53 1.1193681136226772 1104 54-55 1.0091954853548017 1104 56-57 1.2681545158062875 1104 58-59 1.4076663285365854 1104 60-61 1.4922446994469922 1104 62-63 1.5055410534903615 1104 64-65 1.2069267288499788 1104 66-67 1.6834002893733313 1104 68-69 1.4583417808247958 1104 70-71 1.488397607295152 1104 72-73 1.5097839244947373 1104 74-75 1.352156501104261 1104 76-77 1.3940120347003138 1104 78-79 1.3317859256378632 1104 80-81 1.7718123605985916 1104 82-83 1.7637081311405645 1104 84-85 2.0321513093878174 1104 86-87 2.0539555011742507 1104 88-89 2.208848159559441 1104 90-91 1.6506691949216652 1104 92-93 1.5366349048638277 1104 94-95 2.0398662334246858 1104 96-97 2.467833036243185 1104 98-99 2.4800120550076734 1104 100-101 3.2158321189147934 1105 1 0.3143845681650532 1105 2 0.20733919219594554 1105 3 0.31213498653060867 1105 4 0.28887622023648873 1105 5 0.1701507389297774 1105 6 0.21583448729011678 1105 7 0.17958796642962227 1105 8 0.24821744566424542 1105 9 0.3911351121972899 1105 10-11 0.3165135407553521 1105 12-13 0.3708547067487302 1105 14-15 0.43818991532426566 1105 16-17 0.36339245013596866 1105 18-19 0.38381745108064536 1105 20-21 -0.03756340412527237 1105 22-23 0.1968690595873568 1105 24-25 0.24659100207880158 1105 26-27 0.3801015441807891 1105 28-29 0.4978962062184564 1105 30-31 0.42499297644752687 1105 32-33 0.33345284186088264 1105 34-35 0.5541908971962286 1105 36-37 0.6387932783895138 1105 38-39 0.6976237195931105 1105 40-41 0.4047470053409725 1105 42-43 0.2961568471030631 1105 44-45 0.6415112364529989 1105 46-47 0.8521225075119361 1105 48-49 0.8080632633167042 1105 50-51 1.0934662058308575 1105 52-53 0.7920973466375401 1105 54-55 0.9600065920427028 1105 56-57 1.1080646140016128 1105 58-59 1.040984733525974 1105 60-61 1.0691588448822316 1105 62-63 1.0840724361867515 1105 64-65 1.1359507468966967 1105 66-67 1.0081166508382964 1105 68-69 0.9064243179797842 1105 70-71 1.0217873365945103 1105 72-73 1.3829593491231869 1105 74-75 1.5725486189386544 1105 76-77 1.6113604290739865 1105 78-79 1.7944816528140883 1105 80-81 1.8622449508321388 1105 82-83 1.984465164049265 1105 84-85 2.1428781538676454 1105 86-87 2.3206835399215997 1105 88-89 1.8851270210244095 1105 90-91 2.0993488658346173 1105 92-93 2.098363607093127 1105 94-95 2.3775287865032446 1105 96-97 2.194627754926838 1105 98-99 2.435815863394083 1105 100-101 3.5965878248595935 1106 1 -0.03457376516828248 1106 2 -0.09782179265253887 1106 3 0.019047865318484014 1106 4 0.1525125838728556 1106 5 -0.11654433682779342 1106 6 0.08041782062345249 1106 7 0.09507092097507552 1106 8 -0.0020192967599967915 1106 9 0.029392687954867824 1106 10-11 -0.3470754743961564 1106 12-13 -0.4275591190088406 1106 14-15 -0.6157873574030006 1106 16-17 -0.4650876635003911 1106 18-19 -0.14991834437389429 1106 20-21 -0.3865217374586152 1106 22-23 -0.2773260161702211 1106 24-25 -0.22736733125452702 1106 26-27 -0.4185490240010239 1106 28-29 -0.2730554983269968 1106 30-31 -0.1511197129464108 1106 32-33 0.2033628797396645 1106 34-35 -0.03186497401588895 1106 36-37 -0.09925122918624396 1106 38-39 -0.1403260910129447 1106 40-41 0.13651783867431533 1106 42-43 0.21767673346670335 1106 44-45 -0.11535429385003937 1106 46-47 0.4929842499361712 1106 48-49 0.5639818239227665 1106 50-51 0.07429006946722438 1106 52-53 0.4553312481526959 1106 54-55 0.5373029935578515 1106 56-57 0.03621328824404202 1106 58-59 -0.48517530435281486 1106 60-61 0.15178195094284064 1106 62-63 0.27027035285341583 1106 64-65 0.047172337805783116 1106 66-67 0.4940304765958743 1106 68-69 0.07640537858585006 1106 70-71 0.25024377598844794 1106 72-73 0.5633570763959135 1106 74-75 0.37913005833259206 1106 76-77 0.38112842149823223 1106 78-79 0.3984115770565104 1106 80-81 1.1207676781048654 1106 82-83 0.44966402768563185 1106 84-85 -0.05420991431416766 1106 86-87 0.8179610020428107 1106 88-89 1.0043268316304683 1106 90-91 0.4767162900770394 1106 92-93 0.8496656904264626 1106 94-95 1.0169700743820336 1106 96-97 0.615319042805627 1106 98-99 -0.0557797805453113 1106 100-101 1.2052525975868669 1107 1 0.19856872847039853 1107 2 0.07086893138169614 1107 3 0.10113784912602597 1107 4 0.17150980802274773 1107 5 -0.0767282368971891 1107 6 0.019349118333373383 1107 7 0.018069995691703866 1107 8 -0.2093406014095507 1107 9 -0.020283462866323987 1107 10-11 -0.0393030941046959 1107 12-13 0.0028422226520490312 1107 14-15 0.25538139115123926 1107 16-17 0.19291892914360176 1107 18-19 0.38424485248014406 1107 20-21 0.20142355516225763 1107 22-23 0.2791885570428221 1107 24-25 -0.08153399792119842 1107 26-27 -0.10704659513218928 1107 28-29 0.17417443458995763 1107 30-31 0.12272079133302327 1107 32-33 0.39767238702626884 1107 34-35 0.2979985466873316 1107 36-37 0.39480671668467693 1107 38-39 0.48166673804094984 1107 40-41 0.4321603323129892 1107 42-43 0.039964496594159016 1107 44-45 -0.3954838335215598 1107 46-47 -0.14559139197916693 1107 48-49 -0.2781127863016124 1107 50-51 0.0021613394186417167 1107 52-53 0.38492296686654726 1107 54-55 -0.021018773733381124 1107 56-57 0.16461081311103243 1107 58-59 0.3066793104979766 1107 60-61 0.47813228635806126 1107 62-63 -0.05240776419493187 1107 64-65 0.24045535885598213 1107 66-67 0.09161895681794618 1107 68-69 0.5140499541121031 1107 70-71 0.26981930224336637 1107 72-73 0.35246475624786555 1107 74-75 0.5160719968012515 1107 76-77 0.22585430630045167 1107 78-79 0.615549559938767 1107 80-81 0.8872926607558043 1107 82-83 1.044838096619241 1107 84-85 1.2867136341475458 1107 86-87 1.4740013675297412 1107 88-89 1.3867869287852237 1107 90-91 1.8908962576921198 1107 92-93 1.6052318481872767 1107 94-95 1.808289759785687 1107 96-97 1.3398448350795071 1107 98-99 1.7654814963457355 1107 100-101 2.6534222714244784 1108 1 -0.2825896381841524 1108 2 -0.3034494982802478 1108 3 -0.6712479499773281 1108 4 -0.28850473214446026 1108 5 -0.25705660234006444 1108 6 -0.4195821793765475 1108 7 -0.32574437484021956 1108 8 -0.3627696575103627 1108 9 -0.3201744116122285 1108 10-11 -0.30017792749860917 1108 12-13 -0.3101348765845984 1108 14-15 -0.2785759576916078 1108 16-17 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233.0 4 196.0 5 238.0 6 273.0 7 328.0 8 372.0 9 495.0 10 682.0 11 940.0 12 1271.0 13 1872.0 14 2159.0 15 2898.0 16 4029.0 17 5667.0 18 4734.0 19 2962.0 20 2717.0 21 2817.0 22 3030.0 23 3395.0 24 3755.0 25 4182.0 26 4782.0 27 5404.0 28 6546.0 29 7946.0 30 9726.0 31 12313.0 32 16041.0 33 21685.0 34 29897.0 35 43684.0 36 63489.0 37 82461.0 38 55561.0 39 4937.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.591583667985944 18.889871503061407 15.180664086096332 22.337880742856314 2 14.871845649659116 20.03472834368985 41.452552823066576 23.640873183584457 3 18.70319070057264 25.361130499677575 30.43953947257195 25.49613932717783 4 12.289192707389939 16.640462924724016 37.650550761599085 33.419793606286966 5 13.009372969813773 37.77202986019036 34.56478845006677 14.653808719929092 6 30.909841367924074 36.46027439583675 17.789143888851342 14.840740347387833 7 27.514384460920603 33.83268651529194 21.07082174853259 17.582107275254867 8 26.361152544663764 34.22081589503714 20.248215139096057 19.169816421203038 9 26.625327962037264 16.59349120566931 20.798163653961733 35.98301717833169 10-11 24.557522390905813 26.854724930694097 27.28981031242872 21.297942365971377 12-13 25.64323525790964 24.531528726226426 28.16138150446686 21.663854511397073 14-15 22.649115163335427 25.587668407414693 26.793962134808 24.969254294441875 16-17 21.00923797283517 28.991546440272977 27.79851444225211 22.20070114463974 18-19 21.09169104231226 26.786179732978077 31.14724820682132 20.974881017888343 20-21 21.85383713128641 25.77592157279182 31.351575736220454 21.018665559701315 22-23 21.593141632434364 25.60182078846126 31.492592789252903 21.31244478985147 24-25 21.388661612687564 25.90924559586993 31.529564812334222 21.17252797910829 26-27 20.863876304286435 26.328543809300832 31.30685699252004 21.500722893892693 28-29 20.857063891775375 26.331777646628602 31.450457593959737 21.360700867636282 30-31 20.909970841540186 26.216907079133357 31.275223998764147 21.597898080562313 32-33 20.537873704434972 26.379321164958558 31.188540615692084 21.894264514914386 34-35 21.31290980910876 26.384917938611153 30.93881511537111 21.363357136908977 36-37 21.324643008419606 26.065544293746175 30.961152992598905 21.648659705235314 38-39 20.883585504207 26.41525598591592 30.929353944616928 21.77180456526015 40-41 21.081698747031684 26.399453636310984 31.13331853196131 21.385529084696024 42-43 20.93513251290616 26.417143532921266 30.605642267043493 22.042081687129077 44-45 21.092072824162734 26.517924267601163 30.657514773477367 21.732488134758732 46-47 21.44217378396839 26.769831941088874 30.005985725974433 21.782008548968307 48-49 21.2679267325705 27.135910671376728 29.497659251177616 22.098503344875155 50-51 21.489981028881477 27.208590585884135 29.353220021529562 21.948208363704826 52-53 21.580107269927247 27.07141512303214 29.3962807942416 21.95219681279901 54-55 21.40965920061788 27.28953465920062 29.249855184398534 22.05095095578297 56-57 21.350902636843415 27.51757372760903 28.89210105285511 22.23942258269244 58-59 21.396436882978374 26.946570134916836 29.175478747854033 22.481514234250753 60-61 21.16698661988231 27.364627211266857 29.48428207204196 21.984104096808874 62-63 21.38099949325547 27.077169952462537 29.70065876788687 21.841171786395115 64-65 21.251046721800048 27.125599376431325 29.473272697875668 22.15008120389296 66-67 21.233474222985325 27.274032585206463 29.221591711199945 22.270901480608266 68-69 21.52487361092677 27.042435357569467 29.360513519709457 22.0721775117943 70-71 21.453376128191515 27.07490709011053 29.168999469086348 22.302717312611612 72-73 21.229549146005642 27.299461562410247 28.99815854826993 22.47283074331418 74-75 21.239060863353107 27.542006767421768 28.915281726513136 22.30365064271199 76-77 21.291639112406898 27.144887940646917 29.16873826429885 22.394734682647332 78-79 21.055172022652375 27.151354110479197 29.209037298222878 22.584436568645554 80-81 21.37693357515467 27.407262604213052 28.78446165189071 22.431342168741576 82-83 21.448712642873776 27.259465896709784 28.787112794419823 22.504708665996613 84-85 21.09281494922976 27.288280375275086 28.81790278367631 22.80100189181885 86-87 21.307137651606926 27.34040550780175 28.80905611567735 22.54340072491397 88-89 21.364479936673884 27.02569968843743 28.498321520019886 23.1114988548688 90-91 21.16301883423006 26.973527825406002 28.917764797160082 22.945688543203858 92-93 21.117133335424757 26.955701257990643 28.98880525880147 22.938360147783133 94-95 20.84691772144262 26.52967650856088 29.266262050991227 23.357143719005272 96-97 21.054790917011175 26.446397671573063 29.206819240413463 23.2919921710023 98-99 20.923955014962832 26.950719181388166 28.732141133314027 23.393184670334975 100-101 21.270237303840368 26.104025683685766 29.4222256540062 23.203511358467665 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 548.0 1 317.0 2 107.0 3 133.5 4 114.5 5 93.5 6 162.5 7 387.5 8 675.5 9 797.5 10 737.0 11 655.5 12 628.5 13 669.5 14 729.0 15 785.5 16 810.5 17 791.5 18 876.0 19 995.0 20 1031.0 21 1167.0 22 1417.0 23 1636.5 24 1883.5 25 2220.0 26 2706.0 27 3406.5 28 3870.0 29 4371.5 30 5232.5 31 6282.5 32 7690.0 33 8738.0 34 9301.5 35 9973.0 36 11175.0 37 12442.5 38 13189.5 39 13462.5 40 13434.5 41 13534.0 42 13435.5 43 13731.5 44 14108.0 45 14431.5 46 14662.0 47 14888.5 48 15475.5 49 14954.5 50 13891.5 51 13114.5 52 12544.0 53 12327.5 54 12319.0 55 11729.0 56 10623.5 57 9665.0 58 9030.5 59 8027.0 60 6469.5 61 5265.5 62 4287.5 63 3130.0 64 2151.0 65 1567.0 66 1089.5 67 804.5 68 631.5 69 496.0 70 381.5 71 265.5 72 192.5 73 133.0 74 94.5 75 73.5 76 54.0 77 41.5 78 30.0 79 20.5 80 15.0 81 7.5 82 6.0 83 7.0 84 6.5 85 4.5 86 1.5 87 1.5 88 2.5 89 2.0 90 1.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29824138761744157 2 0.24789207419898818 3 0.2536738135389063 4 0.2498193206456276 5 0.2498193206456276 6 0.19465189111057576 7 0.18405203565405925 8 0.18838834015899783 9 0.1924837388581065 10-11 0.1959768730426403 12-13 0.19922910142134426 14-15 0.19621777884847022 16-17 0.1873042640327632 18-19 0.18116116598410023 20-21 0.18164297759576006 22-23 0.18646109371235847 24-25 0.18622018790652853 26-27 0.19103830402312696 28-29 0.1959768730426403 30-31 0.19561551433389546 32-33 0.19320645627559624 34-35 0.1942905324018309 36-37 0.18537701758612385 38-39 0.17622259696458684 40-41 0.17441580342086244 42-43 0.18381112984822934 44-45 0.18296795952782463 46-47 0.1881474343531679 48-49 0.19501324981932067 50-51 0.18935196338231752 52-53 0.19706094916887495 54-55 0.18790652854733797 56-57 0.1693567814984341 58-59 0.15887737894483256 60-61 0.17260900987713804 62-63 0.16622500602264514 64-65 0.17128402794507347 66-67 0.1709226692363286 68-69 0.16983859311009397 70-71 0.17345218019754277 72-73 0.18140207178993015 74-75 0.18357022404239942 76-77 0.18453384726571911 78-79 0.18079980727535533 80-81 0.17935437244037583 82-83 0.169718140207179 84-85 0.16477957118766562 86-87 0.17007949891592386 88-89 0.17754757889665143 90-91 0.17381353890628765 92-93 0.17080221633341364 94-95 0.17224765116839316 96-97 0.17899301373163093 98-99 0.17827029631414115 100-101 0.16225006022645144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 415100.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.62998178684822 #Duplication Level Percentage of deduplicated Percentage of total 1 83.27167448351742 54.65118479713601 2 10.072107873987498 13.2206451264994 3 2.86916532802514 5.64909804665239 4 1.244834360079428 3.2679382551862286 5 0.6481510149541069 2.1269069653282613 6 0.39007011847868994 1.5360176862810084 7 0.27837174336668713 1.2788672712990203 8 0.18694003921775088 0.9815097095274944 9 0.1458272728892363 0.8613577137371691 >10 0.8131187656891519 9.670469384805179 >50 0.05168268506241157 2.3249282559097457 >100 0.026210504552771626 2.7198462473632437 >500 7.383240719090599E-4 0.25899836437121926 >1k 0.00110748610786359 1.4522321759037309 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2628 0.6331004577210311 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2042 0.49192965550469764 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1324 0.31895928691881476 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 536 0.12912551192483737 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 533 0.12840279450734765 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.2045290291496025E-4 0.0 18-19 0.0 0.0 0.0 2.409058058299205E-4 0.0 20-21 0.0 0.0 0.0 2.409058058299205E-4 0.0 22-23 0.0 0.0 0.0 4.81811611659841E-4 0.0 24-25 0.0 0.0 0.0 8.431703204047217E-4 0.0 26-27 0.0 0.0 0.0 9.63623223319682E-4 0.0 28-29 0.0 0.0 0.0 0.002890869669959046 0.0 30-31 0.0 0.0 0.0 0.006624909660322814 0.0 32-33 1.2045290291496025E-4 0.0 0.0 0.00951577933028186 0.0 34-35 2.409058058299205E-4 0.0 0.0 0.011804384485666106 0.0 36-37 2.409058058299205E-4 0.0 0.0 0.01589978318477475 0.0 38-39 3.6135870874488076E-4 0.0 0.0 0.017465670922669235 0.0 40-41 4.81811611659841E-4 0.0 0.0 0.02421103348590701 0.0 42-43 4.81811611659841E-4 0.0 0.0 0.03204047217537943 0.0 44-45 7.227174174897615E-4 0.0 0.0 0.055408335340881715 0.0 46-47 9.63623223319682E-4 0.0 0.0 0.10142134425439653 0.0 48-49 9.63623223319682E-4 0.0 0.0 0.1291255119248374 0.0 50-51 9.63623223319682E-4 0.0 0.0 0.16646591182847506 0.0 52-53 9.63623223319682E-4 0.0 0.0 0.21380390267405447 0.0 54-55 9.63623223319682E-4 0.0 0.0 0.2715008431703204 0.0 56-57 9.63623223319682E-4 0.0 0.0 0.3400385449289328 0.0 58-59 9.63623223319682E-4 0.0 0.0 0.4384485666104553 0.0 60-61 9.63623223319682E-4 0.0 0.0 0.569742230787762 0.0 62-63 9.63623223319682E-4 0.0 0.0 0.7229583232955914 0.0 64-65 9.63623223319682E-4 0.0 0.0 0.8522042881233438 0.0 66-67 9.63623223319682E-4 0.0 0.0 1.0319200192724645 0.0 68-69 9.63623223319682E-4 0.0 0.0 1.245483016140689 0.0 70-71 9.63623223319682E-4 0.0 0.0 1.4115875692604192 0.0 72-73 9.63623223319682E-4 0.0 0.0 1.6909178511202119 2.409058058299205E-4 74-75 9.63623223319682E-4 0.0 0.0 1.93905083112503 2.409058058299205E-4 76-77 9.63623223319682E-4 0.0 0.0 2.1709226692363286 2.409058058299205E-4 78-79 9.63623223319682E-4 0.0 0.0 2.4694049626596 2.409058058299205E-4 80-81 9.63623223319682E-4 0.0 0.0 2.7015177065767286 2.409058058299205E-4 82-83 9.63623223319682E-4 0.0 0.0 2.966875451698386 2.409058058299205E-4 84-85 0.0010840761262346423 0.0 0.0 3.403276318959287 2.409058058299205E-4 86-87 0.0012045290291496024 0.0 0.0 3.8985786557456032 2.409058058299205E-4 88-89 0.0012045290291496024 0.0 0.0 4.478077571669477 2.409058058299205E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGACCG 25 0.005008697 57.029636 5 GGTATCA 1490 0.0 45.319363 1 GTATCAA 2485 0.0 38.272366 1 TATCAAC 3180 0.0 29.886139 2 AGGATAG 80 4.475085E-4 29.702938 5 ATCAACG 3225 0.0 29.46912 3 TCAACGC 3260 0.0 29.156258 4 CAACGCA 3320 0.0 28.486189 5 AGCCGCG 85 6.394314E-4 27.945578 7 AACGCAG 3400 0.0 27.802496 6 TAGTACA 105 6.8709865E-5 27.156971 4 TGGACCG 90 8.9107576E-4 26.402609 5 GTACATG 2190 0.0 26.273804 1 CATGGGG 795 0.0 25.705181 4 GTGTAAG 170 1.1070915E-7 25.175337 1 CGCAGAG 3710 0.0 25.098293 8 ACGCAGA 3750 0.0 25.083952 7 TACATGG 2295 0.0 25.053568 2 GCCGCGC 95 0.0012241051 25.00394 8 GGATAGT 115 1.2794998E-4 24.783522 6 >>END_MODULE