FastQCFastQC Report
Thu 26 May 2016
SRR937700_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937700_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415100
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31520.7593350999759094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26010.6265960009636232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17500.42158516020236086No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC8650.20838352204288124No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.16261141893519634No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA6150.14815707058540112No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA5130.12358467839074921No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.11250301132257287No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4160.10021681522524692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG654.4072294E-851.1581271
GGTATCA17250.042.4093481
AGGTAAG1900.039.9985282
GTATCAA27800.036.5678061
GGTCGGA550.002575155634.5525057
GGTAAGG2350.032.3392373
GTAAGGT2350.032.3392374
GGTGTGC3400.032.1389128
TGTATCG600.003948780231.6655035
TTGCGTA300.003951036431.66550360-61
TAAGGTG2450.031.0192665
GTGCTAT953.483538E-530.002511
TATCGTG804.483385E-429.6935627
GTAAGAC804.4897172E-429.6864093
TATCAAC36700.027.696532
ATCAACG36750.027.5295983
TCAACGC37450.027.0150284
CAACGCA37500.026.9790085
GTGTGCG908.927231E-426.3942789
AACGCAG38750.026.1087176
Produced by FastQC (version 0.11.5)