FastQCFastQC Report
Thu 26 May 2016
SRR937699_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937699_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548923
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58371.0633549696405507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46550.8480242219764886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29170.5314042224501433No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20870.3801990443104042No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA20840.37965251957014007No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18820.34285318705902285No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14500.26415362446099No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12860.23427693865988491No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11410.20786157621378593No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10180.1854540618629571No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8580.1563060757155375No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG8530.15539520114843064No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8460.15411997675448105No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT7420.13517378575865832No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA7390.1346272610183942No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6900.12570069026074696No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6390.11640976967625695No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC5880.10711884909176696No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT5880.10711884909176696No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT5570.10147142677570443No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC550.002577698634.5475126
TGCACCG804.4603102E-429.7218275
GACTCGT650.00583973729.2325127
GTGTAGG1503.2840035E-828.5407771
CTTCGCG350.008392727.14199614-15
TCGCGGT350.00839456827.14075916-17
GGCGGAA1301.00039615E-525.5784478
GTACATG53700.024.714081
GAGTACT41350.024.63948212-13
CATGGGG12450.024.4459044
TACATGG54900.023.9052072
AGGCGGA1601.8556348E-623.7514157
ATGGGAT13500.023.601335
ACATGGG53750.023.4435393
AGGACCG1452.3107199E-522.9575465
CTAGGTC1252.2362403E-422.826364
GTACTTT45500.022.60093114-15
AGTACTT42750.021.94374812-13
CTCCTAA2405.81349E-921.8019831
CATGGGA40000.021.51864
Produced by FastQC (version 0.11.5)