FastQCFastQC Report
Thu 26 May 2016
SRR937698_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937698_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552016
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56521.023883365699545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44970.8146502999913047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29190.5287890206080983No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21320.3862206892553839No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA20920.3789745224776093No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18570.3364032926581838No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15190.27517318338598884No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT13150.23821773281933858No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11380.20615344482768616No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9720.17608185269992174No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9040.163763369177705No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8500.15398104402770935No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG8400.1521695023332657No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA7610.13785832294716097No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT7330.13278600620271877No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6970.12626445610272166No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6670.12082983101939075No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT6090.11032288919161763No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC6080.11014173502217327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGCG250.001617331437.9926194-95
GTAAGCG452.4160827E-531.66050594-95
TACCTGG3250.030.7128332
CATTCCG1004.9443246E-528.5035179
TAAGGCG950.001220974525.0167185
GACAGTG3050.024.923377
GTACATG49500.024.206791
GAGTACT42200.023.80489212-13
GTACTAG1000.001651368823.752936
TACATGG50850.023.275142
GTGTAAG1652.4043948E-623.0540851
GTATAGA1252.2381305E-422.8235441
ATGGGAT13700.022.5515655
AGTACTT42800.022.19496712-13
GTACTTT45450.021.99716814-15
ACATGGG52100.021.9848633
GTACCTG4850.021.568641
GTATAGC2457.408744E-921.3485531
CATGGGA37600.021.1111834
GTATCAA93250.020.9062841
Produced by FastQC (version 0.11.5)